FastQCFastQC Report
Thu 26 May 2016
SRR938671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938671_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019116
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73930.7254326298478289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63300.6211265449664218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43740.42919549884409625No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31650.3105632724832109No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT24980.24511439325847106No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15360.15071885830464835No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT15330.15042448553452206RNA PCR Primer, Index 33 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.10253984826064944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30600.056.5508881
GTATCAA56000.039.8147771
GTGGTAT11450.031.5549561
TATCAAC72650.030.624552
ATCAACG73250.030.293923
TCAACGC73300.030.2732544
CAACGCA75750.029.1686635
AACGCAG77700.028.3559866
CGCAGAG88100.024.9008338
ACGCAGA88200.024.8725997
GCAGAGT93350.023.195219
TGGTATC15300.022.993222
GTAGCCC5200.022.8445643
GTGTAGC5650.022.7182461
GAGTACT62150.022.69149612-13
CGTCAGA1050.002185325122.6381112
ACAGCGC1703.1575928E-622.3454388
GTACATG47800.021.6814371
GTACTTT64300.021.60044514-15
AGTACTT62600.021.39058112-13
Produced by FastQC (version 0.11.5)