##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938671_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1019116 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77059628148317 31.0 31.0 34.0 30.0 34.0 2 31.99725153956959 31.0 31.0 34.0 30.0 34.0 3 32.0345750630939 33.0 31.0 34.0 30.0 34.0 4 35.72866876783409 37.0 35.0 37.0 33.0 37.0 5 35.63438018832007 37.0 35.0 37.0 33.0 37.0 6 35.63519952586359 37.0 35.0 37.0 33.0 37.0 7 35.57540456631041 37.0 35.0 37.0 33.0 37.0 8 35.57420843162113 37.0 35.0 37.0 33.0 37.0 9 37.13530844378854 39.0 37.0 39.0 33.0 39.0 10-11 37.05440155978319 39.0 37.0 39.0 33.0 39.0 12-13 36.96689631013545 39.0 37.0 39.0 33.0 39.0 14-15 37.973530981752816 40.0 37.0 41.0 33.0 41.0 16-17 37.90133360677293 40.0 37.0 41.0 32.5 41.0 18-19 38.17349791387831 40.0 37.5 41.0 33.0 41.0 20-21 38.188404460336216 40.0 37.0 41.0 33.0 41.0 22-23 38.05961637340597 40.0 37.0 41.0 33.0 41.0 24-25 37.94954548844292 40.0 37.0 41.0 33.0 41.0 26-27 37.759282554684646 40.0 37.0 41.0 32.0 41.0 28-29 37.63646189442615 39.5 37.0 41.0 32.0 41.0 30-31 37.35188290636198 39.0 36.0 41.0 32.0 41.0 32-33 37.084448679051256 39.0 35.5 41.0 31.0 41.0 34-35 36.90626287880869 39.0 35.0 41.0 31.0 41.0 36-37 36.82670422209052 39.0 35.0 41.0 31.0 41.0 38-39 36.63345880154958 39.0 35.0 41.0 30.0 41.0 40-41 36.44492727030092 39.0 35.0 41.0 30.0 41.0 42-43 36.18543865467719 39.0 35.0 40.5 30.0 41.0 44-45 35.77785257026678 39.0 35.0 40.0 28.5 41.0 46-47 35.249327848841546 38.5 35.0 40.0 26.5 41.0 48-49 34.80986511839673 38.0 34.5 40.0 24.0 41.0 50-51 34.41838662134634 38.0 34.0 40.0 23.5 41.0 52-53 33.98242054878934 38.0 33.0 40.0 22.0 41.0 54-55 33.67684149792565 38.0 33.0 40.0 19.5 41.0 56-57 33.35380221682321 38.0 33.0 40.0 17.5 41.0 58-59 32.910973333752 37.5 32.0 40.0 13.5 41.0 60-61 32.5537303898673 37.0 31.5 40.0 8.5 41.0 62-63 32.09387891074225 36.5 31.0 39.0 7.5 40.5 64-65 31.61902472338772 36.0 31.0 39.0 4.0 40.0 66-67 31.15116483305139 35.5 30.0 39.0 2.0 40.0 68-69 30.678616565729513 35.0 29.0 38.0 2.0 40.0 70-71 30.195460085014858 35.0 29.0 38.0 2.0 40.0 72-73 29.726420741112886 34.0 28.0 37.5 2.0 39.0 74-75 29.17004197755702 34.0 28.0 37.0 2.0 39.0 76-77 27.879464653680248 32.5 26.0 35.0 2.0 37.5 78-79 28.538810596634733 34.0 28.0 36.0 2.0 38.0 80-81 28.162188602671336 34.0 26.5 35.5 2.0 37.0 82-83 27.748116504892476 33.0 26.0 35.0 2.0 37.0 84-85 27.228336617225125 33.0 26.0 35.0 2.0 36.0 86-87 26.66295053752468 32.5 25.0 35.0 2.0 36.0 88-89 25.988211842420295 32.0 23.5 34.5 2.0 35.5 90-91 25.448931721217214 32.0 21.0 34.0 2.0 35.0 92-93 24.725462557746127 31.0 18.5 34.0 2.0 35.0 94-95 24.01415736775794 31.0 10.5 34.0 2.0 35.0 96-97 23.064358718732706 31.0 2.0 34.0 2.0 35.0 98-99 21.847889739735223 30.0 2.0 34.0 2.0 35.0 100-101 18.047517652553783 23.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18771650210074853 1101 2 0.2567119316045918 1101 3 0.2506907945369292 1101 4 0.1663689616573052 1101 5 -0.012069801554773107 1101 6 0.1345218893617144 1101 7 0.015224992643105395 1101 8 0.01307803766356841 1101 9 0.12900348586643418 1101 10-11 0.09083360648666883 1101 12-13 0.12017085621460666 1101 14-15 0.2396767463353484 1101 16-17 0.27159414646269653 1101 18-19 0.21505199960306243 1101 20-21 0.3386069258472375 1101 22-23 0.33787400030088577 1101 24-25 0.3806729131469879 1101 26-27 0.24489120382447993 1101 28-29 0.24752697328284512 1101 30-31 0.367797825562171 1101 32-33 0.4596618592663546 1101 34-35 0.2626763886697532 1101 36-37 0.2739418075057287 1101 38-39 0.5156025661389307 1101 40-41 0.6134207560124523 1101 42-43 0.5142528498830004 1101 44-45 0.5623694185706043 1101 46-47 0.38842843702945373 1101 48-49 0.680706163834742 1101 50-51 0.6506682068882199 1101 52-53 0.4468022908006972 1101 54-55 0.8072272812708903 1101 56-57 0.6521787832714878 1101 58-59 0.844654859052028 1101 60-61 1.041117670709795 1101 62-63 0.5540624295963923 1101 64-65 0.6401512809355836 1101 66-67 0.8093648684890447 1101 68-69 0.7526840201524116 1101 70-71 0.62136297082046 1101 72-73 0.8429801072619618 1101 74-75 0.6900846025068397 1101 76-77 0.7568166303118744 1101 78-79 0.8260949353713052 1101 80-81 0.8355265862366359 1101 82-83 0.8614617026330187 1101 84-85 1.0434484511194775 1101 86-87 1.069761464685584 1101 88-89 0.9223586913980419 1101 90-91 0.9851861343463426 1101 92-93 1.1484535556983317 1101 94-95 1.0724162597199083 1101 96-97 0.6156416159195857 1101 98-99 0.8253185942535346 1101 100-101 1.5247373603821508 1102 1 0.27406618448675246 1102 2 0.2964939704739322 1102 3 0.33585385213250873 1102 4 0.21584354073020506 1102 5 0.08411528829810777 1102 6 0.21056859869177202 1102 7 0.28383788139555577 1102 8 0.20282937778211618 1102 9 0.05814722686343998 1102 10-11 0.2338342913049516 1102 12-13 0.4732200686735837 1102 14-15 0.6713324716523701 1102 16-17 0.461177824173042 1102 18-19 0.38511009108534466 1102 20-21 0.45103696811705873 1102 22-23 0.5199589177684771 1102 24-25 0.5617996753733578 1102 26-27 0.45213551865198554 1102 28-29 0.4439021592464272 1102 30-31 0.3063477818754947 1102 32-33 0.5542624212896428 1102 34-35 0.570478594256933 1102 36-37 0.5429714908363152 1102 38-39 0.4942899583981273 1102 40-41 0.47264336918457417 1102 42-43 0.376506374335726 1102 44-45 0.46036101183576505 1102 46-47 0.5467188708264175 1102 48-49 0.6276599031787242 1102 50-51 0.6734490414219536 1102 52-53 0.744833438225541 1102 54-55 0.5844564828672176 1102 56-57 0.6686592895647365 1102 58-59 0.802570413872914 1102 60-61 0.7003219827035565 1102 62-63 0.8958658631336007 1102 64-65 0.7453865986676576 1102 66-67 0.40932118180521826 1102 68-69 0.5178976126146893 1102 70-71 0.5682399632874606 1102 72-73 0.7325035681918983 1102 74-75 0.8934484771614777 1102 76-77 0.6860199977286925 1102 78-79 1.030453567623887 1102 80-81 0.5295007756715435 1102 82-83 0.5795798692091623 1102 84-85 0.8155739853958117 1102 86-87 0.5251119027331477 1102 88-89 0.44284774611266187 1102 90-91 0.7972820325209504 1102 92-93 1.2670587697630857 1102 94-95 1.1035672267305578 1102 96-97 0.9636001489915849 1102 98-99 0.9942957354914022 1102 100-101 1.7207358962878843 1103 1 0.24554698285313137 1103 2 0.2066700677885578 1103 3 0.21681031086382063 1103 4 0.156165873470691 1103 5 0.11706546724986566 1103 6 0.15907550425355055 1103 7 0.14011546521743412 1103 8 0.18314248846871806 1103 9 0.25956017726196023 1103 10-11 0.3751765392903721 1103 12-13 0.2644355134181069 1103 14-15 0.40763827056768065 1103 16-17 0.40927742235498243 1103 18-19 0.44399839444700007 1103 20-21 0.3762356814209724 1103 22-23 0.37658342647453225 1103 24-25 0.29674082988666584 1103 26-27 0.36091238886255184 1103 28-29 0.6064167059385639 1103 30-31 0.5492726001790729 1103 32-33 0.5995064763584779 1103 34-35 0.6995983272565169 1103 36-37 0.638059649279981 1103 38-39 0.636588994473918 1103 40-41 0.7236588929752727 1103 42-43 0.9604237710816577 1103 44-45 0.9407696366518721 1103 46-47 0.9585357252277475 1103 48-49 1.1189132242159872 1103 50-51 0.9944577584586014 1103 52-53 0.726092358366941 1103 54-55 0.5612822299633748 1103 56-57 0.8129163652953579 1103 58-59 0.9583978736362155 1103 60-61 0.7836844094211699 1103 62-63 1.2976806315941687 1103 64-65 1.1425365448723426 1103 66-67 0.9905378341996602 1103 68-69 1.0792023136664426 1103 70-71 0.8461619271457899 1103 72-73 0.9023683114016094 1103 74-75 0.8161336318504517 1103 76-77 0.7889057320896242 1103 78-79 0.9860828630150564 1103 80-81 0.8765239186353391 1103 82-83 1.221471048973342 1103 84-85 1.114120414045658 1103 86-87 1.3102155410529726 1103 88-89 1.2788631601170088 1103 90-91 1.4859844675823197 1103 92-93 1.5585047658546927 1103 94-95 1.378684149199433 1103 96-97 1.706787905617194 1103 98-99 2.173245848571348 1103 100-101 2.386138921999848 1104 1 0.24868320576334568 1104 2 0.2087918428143567 1104 3 0.1923644904303785 1104 4 0.0612690726450964 1104 5 0.0205195436172545 1104 6 0.1020579603938998 1104 7 0.14356128565087545 1104 8 0.028084696931053088 1104 9 0.21008339707620394 1104 10-11 0.1901321636453801 1104 12-13 0.2476775154820885 1104 14-15 0.4154814078225826 1104 16-17 0.39531612204538646 1104 18-19 0.3355675378938514 1104 20-21 0.31572845747876244 1104 22-23 0.34989508798123836 1104 24-25 0.3905230796286716 1104 26-27 0.372869561205178 1104 28-29 0.3161468400974954 1104 30-31 0.44528395208825344 1104 32-33 0.4501241234173037 1104 34-35 0.43325519000160995 1104 36-37 0.5795034057299304 1104 38-39 0.38924740520663903 1104 40-41 0.5406965606739362 1104 42-43 0.612453182846366 1104 44-45 0.6733078203464018 1104 46-47 0.5691337644434356 1104 48-49 0.7142450095617079 1104 50-51 0.8947575520602484 1104 52-53 0.951950459502136 1104 54-55 1.016892653079985 1104 56-57 0.9185422682881423 1104 58-59 1.0285810521707859 1104 60-61 1.2028113444056885 1104 62-63 1.0610041610059326 1104 64-65 0.8007589390931855 1104 66-67 0.9719913945711802 1104 68-69 1.014216761550859 1104 70-71 1.0167186866917177 1104 72-73 0.6746737809578534 1104 74-75 0.9528420941827562 1104 76-77 0.8851157424095426 1104 78-79 0.8201876101770793 1104 80-81 0.8075326905651607 1104 82-83 0.6982713585708638 1104 84-85 0.7859356875234695 1104 86-87 0.877143817626763 1104 88-89 1.0225179588786197 1104 90-91 1.1288565006060551 1104 92-93 1.328271535720532 1104 94-95 1.4964395671560915 1104 96-97 1.3987703617575455 1104 98-99 1.9258702035765083 1104 100-101 2.2521507899049027 1105 1 0.1690923408459497 1105 2 0.30840700316421277 1105 3 0.13969976739831225 1105 4 0.12215828547308405 1105 5 0.08735433467654019 1105 6 0.11007545310526723 1105 7 0.23957780654494343 1105 8 0.2175803237532108 1105 9 0.348822950040244 1105 10-11 0.1403051471244865 1105 12-13 0.31495496932076605 1105 14-15 0.4979886964523246 1105 16-17 0.3094254514914425 1105 18-19 0.22429397132436435 1105 20-21 0.19759580441753855 1105 22-23 0.2648620461931017 1105 24-25 0.16129567438085957 1105 26-27 0.17160960007787196 1105 28-29 0.35713955146776044 1105 30-31 0.41697005510089014 1105 32-33 0.39070867152226185 1105 34-35 0.16804284792192448 1105 36-37 0.4372108887231221 1105 38-39 0.25215702619789226 1105 40-41 0.26203815445430934 1105 42-43 0.49435162793869125 1105 44-45 0.5808301721442675 1105 46-47 0.48837623285938037 1105 48-49 0.5767459813790055 1105 50-51 0.6543683392322635 1105 52-53 0.5698644536712294 1105 54-55 0.6135918756261489 1105 56-57 0.6027390612910537 1105 58-59 0.5027720142699152 1105 60-61 0.7797851053386324 1105 62-63 0.7530056187318834 1105 64-65 0.8175057806869717 1105 66-67 0.7153164674574732 1105 68-69 0.7970262853603849 1105 70-71 0.6861069277024043 1105 72-73 0.7453987566624214 1105 74-75 0.7685461758154091 1105 76-77 0.7062260436729062 1105 78-79 0.8149422263092454 1105 80-81 0.9258052853173488 1105 82-83 0.9573097453541486 1105 84-85 0.999396328922888 1105 86-87 1.1561102899299662 1105 88-89 1.2137604272945595 1105 90-91 1.5119828368548411 1105 92-93 1.495070369539775 1105 94-95 1.617134416524408 1105 96-97 1.4950084569956381 1105 98-99 1.4908721472111033 1105 100-101 1.3671794585152028 1106 1 -0.06084677489429424 1106 2 0.03701621418766621 1106 3 -0.02758908790617909 1106 4 0.02222651945361065 1106 5 -0.07381507920672448 1106 6 -0.14020509569894557 1106 7 0.06149745586363764 1106 8 0.14402860408772256 1106 9 -0.2841158292294068 1106 10-11 0.11120373037652342 1106 12-13 0.050927999041086025 1106 14-15 -0.04593058444047671 1106 16-17 0.10276389767400929 1106 18-19 0.04574838895768352 1106 20-21 0.08822265477082425 1106 22-23 0.19210591970271196 1106 24-25 0.12915944326503137 1106 26-27 0.04657459021871091 1106 28-29 0.027751288840242694 1106 30-31 -0.13035434578408456 1106 32-33 -0.061404890122346956 1106 34-35 0.010517278976472255 1106 36-37 -0.024436632954788706 1106 38-39 0.030605238862342787 1106 40-41 0.10610565158302876 1106 42-43 0.20174815383283118 1106 44-45 0.13589292672937603 1106 46-47 -0.34230434000529897 1106 48-49 -0.019632166453760647 1106 50-51 0.2939519427759194 1106 52-53 0.42041370901857533 1106 54-55 0.19262862986915508 1106 56-57 0.12194749072526889 1106 58-59 -0.03176227471509918 1106 60-61 0.1353800097828639 1106 62-63 -0.18072988154725422 1106 64-65 -0.0953290686437569 1106 66-67 0.09481441197929996 1106 68-69 0.02756879249863431 1106 70-71 -0.026827734971725192 1106 72-73 0.11035656625766066 1106 74-75 0.06830340946321911 1106 76-77 0.12272212538826466 1106 78-79 0.5227649796161487 1106 80-81 0.016493038727634968 1106 82-83 0.36072106843547047 1106 84-85 0.5666378151830465 1106 86-87 0.96713317932889 1106 88-89 0.7795982296716595 1106 90-91 0.6196708139522826 1106 92-93 0.8143024834961601 1106 94-95 1.127938600038103 1106 96-97 1.3088612708484497 1106 98-99 1.452854729688692 1106 100-101 1.642496051026761 1107 1 0.04013030787926297 1107 2 0.13934566066164678 1107 3 0.13203698353065363 1107 4 0.043757573105075664 1107 5 0.041052202310908115 1107 6 0.053069919915522235 1107 7 0.10529357635924441 1107 8 0.11453639886297395 1107 9 0.10363353507068496 1107 10-11 0.038507508631582255 1107 12-13 0.05281596994390725 1107 14-15 -0.0038378194083250605 1107 16-17 0.11045457650720181 1107 18-19 0.24632972740626968 1107 20-21 0.08511622196817825 1107 22-23 0.1580855203639473 1107 24-25 0.17973651109141286 1107 26-27 0.20516992919045407 1107 28-29 0.3212475760680533 1107 30-31 0.06036674877638859 1107 32-33 0.2289507103538284 1107 34-35 0.46388030499701216 1107 36-37 0.41091370931778926 1107 38-39 0.3099484171661615 1107 40-41 0.30498496913759965 1107 42-43 0.32351160050966143 1107 44-45 0.31178206137676767 1107 46-47 0.08265860344987885 1107 48-49 0.06432826623144194 1107 50-51 0.11731366981553748 1107 52-53 0.19804107587747666 1107 54-55 -0.025415994574103706 1107 56-57 0.0782621394932832 1107 58-59 0.11259899145321128 1107 60-61 0.05618576942868003 1107 62-63 0.1623919254953563 1107 64-65 0.0014926097463643373 1107 66-67 0.25777290331082625 1107 68-69 0.45122964103567753 1107 70-71 0.4490107750081833 1107 72-73 0.4192257588787349 1107 74-75 0.2887008798313282 1107 76-77 0.09971325850889912 1107 78-79 0.4736443719066088 1107 80-81 0.4069876123682903 1107 82-83 0.6399930697024168 1107 84-85 0.7596734980110504 1107 86-87 0.7406852159708279 1107 88-89 0.5679324023008832 1107 90-91 0.7284066386005392 1107 92-93 0.7969081502559519 1107 94-95 0.9472997327494674 1107 96-97 0.9600279514539558 1107 98-99 0.6362299091882839 1107 100-101 0.8538927402268897 1108 1 0.1308106188907594 1108 2 0.11510072312324127 1108 3 0.13548418155006914 1108 4 0.11630768268370417 1108 5 -0.09421790812019282 1108 6 0.11943247776842014 1108 7 0.10850723159681763 1108 8 0.06635110619745888 1108 9 0.24704478703759492 1108 10-11 0.14269200920522707 1108 12-13 0.19226631857088705 1108 14-15 0.2215624889036647 1108 16-17 0.24942229965156315 1108 18-19 0.25811386994018193 1108 20-21 0.31542536867566895 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-1.3825799220474515 2316 82-83 -1.3302917044922005 2316 84-85 -1.4256949431071604 2316 86-87 -1.6752570832741398 2316 88-89 -1.9070180771574137 2316 90-91 -1.661837193087635 2316 92-93 -2.0625032390542444 2316 94-95 -1.7868487211321984 2316 96-97 -2.0655449535577723 2316 98-99 -2.0891478144415103 2316 100-101 -2.644646507392393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 11.0 5 46.0 6 102.0 7 285.0 8 608.0 9 1101.0 10 1926.0 11 3134.0 12 4240.0 13 6006.0 14 7351.0 15 10185.0 16 13261.0 17 16916.0 18 14858.0 19 10930.0 20 9619.0 21 9782.0 22 10522.0 23 11310.0 24 12765.0 25 14373.0 26 16335.0 27 19357.0 28 23317.0 29 27460.0 30 33002.0 31 39534.0 32 47284.0 33 58456.0 34 74470.0 35 97614.0 36 132918.0 37 180091.0 38 105471.0 39 4469.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.92363947864466 22.170848571257554 16.058965001918335 21.846546948179444 2 15.173128786532583 22.68291940619119 40.50042363022138 21.64352817705485 3 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18.979537167505956 22-23 19.82237547050581 28.905247292751756 31.772290887396526 19.500086349345906 24-25 19.470076889976827 29.026493702244288 32.30301451297699 19.20041489480189 26-27 19.34398481023837 29.2159561184664 32.224667172010804 19.215391899284423 28-29 19.05578906414435 29.219455498419954 32.367921006009624 19.356834431426076 30-31 19.940506416681753 28.855908856968615 32.25247926727612 18.95110545907351 32-33 19.193614067107244 29.157651491735475 32.32814746127768 19.3205869798796 34-35 19.527571109424287 29.04018162717656 32.2720423391123 19.160204924286845 36-37 19.42035059273738 28.986641632507244 31.73418315994757 19.85882461480781 38-39 19.059067231421928 29.199050716599068 32.126236833157606 19.615645218821403 40-41 19.625379558566458 28.65290009974375 32.27296522510049 19.448755116589304 42-43 19.390231756481278 28.62261476574869 32.05365277593112 19.93350070183891 44-45 19.690895200823913 28.827489022751323 31.71677839769705 19.764837378727716 46-47 19.82973478975897 28.69153266164009 30.978367526365986 20.500365022234956 48-49 19.705215107995556 28.742753523642055 31.075265229865884 20.4767661384965 50-51 19.724720454475207 28.93938286317613 30.5439700051859 20.79192667716276 52-53 19.781359531201552 29.225132369622298 30.53298152516495 20.4605265740112 54-55 20.075124962957585 28.948966835508124 30.09109981140259 20.884808390131703 56-57 20.148295189163942 29.274047311591616 29.669929625283086 20.907727873961353 58-59 19.730055737836853 29.35530981659219 29.93815740270906 20.976477042861895 60-61 20.13303686724573 29.466518041125838 29.41608217317754 20.984362918450895 62-63 19.702010150849635 29.43265543243169 29.751684938981064 21.11364947773761 64-65 19.698395703031842 29.308033457938603 29.52399084941278 21.469579989616772 66-67 19.687494940167635 29.54480889411813 29.237661736774882 21.530034428939345 68-69 19.838597844536338 28.986633730655857 29.375074525575705 21.799693899232096 70-71 19.656152979641178 28.90254887569227 29.527011645386786 21.91428649927977 72-73 19.69977887687902 28.644250823385576 29.49096544994164 22.165004849793768 74-75 19.701208641128936 28.930537364132768 28.894222676755604 22.47403131798269 76-77 19.281060830671176 28.762475481004284 29.64383397571957 22.312629712604974 78-79 19.254473961114428 28.80026580707981 29.24687666865597 22.698383563149793 80-81 19.219825229701332 28.78527477294878 29.09641812169533 22.898481875654557 82-83 19.419104936484082 28.811470461490433 29.04941588521293 22.72000871681255 84-85 19.37630466081027 28.509494642978066 29.081508975477526 23.03269172073413 86-87 19.08468074520508 28.60165364518806 29.06027988614568 23.253385723461186 88-89 19.470054738781545 28.491273075659535 28.809825579368287 23.228846606190633 90-91 19.242868149718554 28.363342804842738 28.976929758697352 23.41685928674136 92-93 19.23743211689292 28.359459271117256 28.801429458986483 23.601679153003346 94-95 19.197471215743164 28.30861297204292 28.761534682692247 23.73238112952167 96-97 19.2556613654814 28.24326619240348 28.485731366256893 24.015341075858224 98-99 19.124326234867898 28.08194162174899 28.41497059487693 24.37876154850618 100-101 18.982051001476492 28.319710923917818 28.683215068542218 24.015023006063473 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2114.0 1 1262.0 2 433.0 3 443.5 4 372.0 5 347.0 6 503.0 7 895.0 8 1246.0 9 1296.5 10 1060.0 11 844.5 12 873.5 13 923.5 14 1023.0 15 1170.0 16 1351.0 17 1536.0 18 1770.5 19 2088.5 20 2454.0 21 2848.0 22 3376.5 23 4131.0 24 5020.0 25 6402.5 26 8487.0 27 10900.0 28 12644.5 29 14678.5 30 17625.5 31 20727.0 32 24112.0 33 27596.0 34 31603.5 35 35707.0 36 39397.5 37 42231.5 38 42293.0 39 40703.0 40 39292.0 41 38263.0 42 38100.0 43 38752.0 44 39480.0 45 39565.0 46 38669.5 47 38129.5 48 37605.5 49 35930.0 50 34097.0 51 31600.0 52 28031.0 53 24631.5 54 21487.5 55 18216.0 56 15305.5 57 12565.0 58 9908.5 59 7466.5 60 5508.5 61 3940.5 62 2725.5 63 1829.5 64 1212.5 65 872.5 66 620.0 67 404.5 68 286.5 69 241.5 70 189.5 71 146.0 72 122.5 73 114.0 74 95.5 75 81.5 76 63.0 77 39.0 78 23.5 79 16.0 80 15.0 81 12.5 82 8.0 83 4.0 84 3.5 85 2.0 86 3.5 87 4.5 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.943727701262663E-4 2 0.05288897436601917 3 0.0 4 0.0 5 0.04386154274881368 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006132766044297214 26-27 8.340561820244212E-4 28-29 4.906212835437772E-5 30-31 0.0014228017222769538 32-33 9.812425670875543E-5 34-35 0.010352109082773698 36-37 0.023697007995164436 38-39 0.003090914086325796 40-41 4.415591551893994E-4 42-43 7.359319253156658E-4 44-45 0.0075065056382197905 46-47 0.0 48-49 0.0 50-51 6.378076686069103E-4 52-53 0.0 54-55 0.0013737395939225762 56-57 0.0 58-59 0.02345169735339255 60-61 0.0 62-63 0.008634934590370479 64-65 0.016190502356944646 66-67 0.005936517530879704 68-69 0.0167301857688428 70-71 0.0 72-73 4.906212835437772E-5 74-75 0.023991380765290702 76-77 0.05082836497513531 78-79 0.0325772532273068 80-81 0.028750407215665342 82-83 0.038759081399958394 84-85 0.01697549641061469 86-87 0.009272742258977389 88-89 0.08993088127357435 90-91 0.02659167356807272 92-93 0.02639542505465521 94-95 0.02811259954705843 96-97 0.04052531802071599 98-99 0.057500814431330684 100-101 0.014277079351123916 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1019116.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.12827018873126 #Duplication Level Percentage of deduplicated Percentage of total 1 75.22660607536152 43.72792483329866 2 14.318547936234971 16.64624846295533 3 4.997344730482602 8.714610141591752 4 2.198027875075381 5.110702328189784 5 1.099302488007619 3.1950276021025688 6 0.6044590004023346 2.1081693656038394 7 0.37214907620976995 1.514266743668577 8 0.23897517480519043 1.111297082358031 9 0.156437219217197 0.8184082451607908 >10 0.6853983965346578 7.177556475293937 >50 0.05990570124559632 2.4068849322341928 >100 0.040147345877137725 4.196435607584931 >500 0.0013494902730003696 0.5392993045414576 >1k 0.0010121177047502773 1.3875680833756665 >5k 3.373725682500924E-4 1.3456007920404403 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7393 0.7254326298478289 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6330 0.6211265449664218 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4374 0.42919549884409625 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3165 0.3105632724832109 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 2498 0.24511439325847106 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1536 0.15071885830464835 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1533 0.15042448553452206 RNA PCR Primer, Index 33 (95% over 22bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1045 0.10253984826064944 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.906212835437772E-5 0.0 18-19 0.0 0.0 0.0 2.4531064177188856E-4 0.0 20-21 0.0 0.0 0.0 8.831183103787988E-4 0.0 22-23 0.0 0.0 0.0 0.003139976214680174 0.0 24-25 0.0 0.0 0.0 0.006279952429360348 0.0 26-27 0.0 0.0 0.0 0.012118345703531295 0.0 28-29 0.0 0.0 0.0 0.026935108466553365 0.0 30-31 0.0 0.0 0.0 0.06755855074397811 0.0 32-33 0.0 0.0 0.0 0.14051393560693778 0.0 34-35 0.0 0.0 0.0 0.25831210578579866 0.0 36-37 0.0 0.0 0.0 0.4463672437681285 0.0 38-39 0.0 0.0 0.0 0.6822088947676221 0.0 40-41 0.0 0.0 0.0 0.9154502529643338 0.0 42-43 0.0 0.0 0.0 1.1404982357258644 0.0 44-45 0.0 0.0 0.0 1.429081674706314 0.0 46-47 0.0 0.0 0.0 1.7671197390679767 0.0 48-49 0.0 0.0 0.0 2.097651297791419 0.0 50-51 0.0 0.0 0.0 2.426220371380687 0.0 52-53 0.0 0.0 0.0 2.738795191126427 0.0 54-55 0.0 0.0 0.0 3.104798668650085 0.0 56-57 0.0 0.0 0.0 3.532620427900259 0.0 58-59 0.0 0.0 0.0 3.9962084787207734 0.0 60-61 0.0 0.0 0.0 4.441937914820295 0.0 62-63 0.0 0.0 0.0 4.8956644778415805 0.0 64-65 0.0 0.0 0.0 5.389965421011936 0.0 66-67 0.0 0.0 0.0 5.961146719313602 0.0 68-69 0.0 0.0 0.0 6.571921155197249 0.0 70-71 0.0 0.0 0.0 7.236320497372232 0.0 72-73 0.0 0.0 0.0 7.87790594986243 0.0 74-75 0.0 0.0 0.0 8.547015256359433 0.0 76-77 0.0 0.0 0.0 9.27769753394118 0.0 78-79 0.0 0.0 0.0 10.061661282915782 0.0 80-81 0.0 0.0 0.0 10.897238390919188 0.0 82-83 0.0 0.0 0.0 11.734827046185124 0.0 84-85 0.0 0.0 0.0 12.56535075496803 0.0 86-87 0.0 0.0 0.0 13.448616251731892 0.0 88-89 0.0 0.0 0.0 14.382415740700765 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3060 0.0 56.550888 1 GTATCAA 5600 0.0 39.814777 1 GTGGTAT 1145 0.0 31.554956 1 TATCAAC 7265 0.0 30.62455 2 ATCAACG 7325 0.0 30.29392 3 TCAACGC 7330 0.0 30.273254 4 CAACGCA 7575 0.0 29.168663 5 AACGCAG 7770 0.0 28.355986 6 CGCAGAG 8810 0.0 24.900833 8 ACGCAGA 8820 0.0 24.872599 7 GCAGAGT 9335 0.0 23.19521 9 TGGTATC 1530 0.0 22.99322 2 GTAGCCC 520 0.0 22.844564 3 GTGTAGC 565 0.0 22.718246 1 GAGTACT 6215 0.0 22.691496 12-13 CGTCAGA 105 0.0021853251 22.638111 2 ACAGCGC 170 3.1575928E-6 22.345438 8 GTACATG 4780 0.0 21.681437 1 GTACTTT 6430 0.0 21.600445 14-15 AGTACTT 6260 0.0 21.390581 12-13 >>END_MODULE