FastQCFastQC Report
Thu 26 May 2016
SRR938670_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938670_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017408
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77300.7597738567025225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60610.595729540164811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42140.4141897842360194No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30190.2967344467509593No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.14615572120525885No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11100.10910077373089261No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10940.10752814996540228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.051.0718421
GTATCAA55350.039.6968081
GTGGTAT13200.032.1357461
TATCAAC70000.031.4427782
ATCAACG71150.031.1350083
TCAACGC71700.030.7635734
CAACGCA73300.029.9594085
AACGCAG74000.029.6701646
TACACCG856.380702E-427.9605875
ACGCAGA84850.025.9257937
CGCAGAG85150.025.7228538
TGGTATC16450.025.7194712
CATTCCG2501.4551915E-1124.702579
GCAGAGT89300.023.8854649
GTACATG46450.022.6766471
GAGTACT59350.022.09159912-13
TACATGG49650.021.4470082
TCGCCGG1005.3493386E-721.39195494-95
GGACGTG4000.021.385646
CATGGGT13850.021.2804034
Produced by FastQC (version 0.11.5)