##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938670_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1017408 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.103853124803422 31.0 31.0 34.0 28.0 34.0 2 31.198329480405107 31.0 31.0 34.0 28.0 34.0 3 31.229681700949865 31.0 31.0 34.0 27.0 34.0 4 34.879871202113605 37.0 35.0 37.0 32.0 37.0 5 34.92023062527521 37.0 35.0 37.0 32.0 37.0 6 34.968880724350505 37.0 35.0 37.0 32.0 37.0 7 34.905810648235516 37.0 35.0 37.0 32.0 37.0 8 34.89016402465874 37.0 35.0 37.0 32.0 37.0 9 36.397929837390706 39.0 37.0 39.0 32.0 39.0 10-11 36.27688596905077 39.0 35.0 39.0 32.0 39.0 12-13 36.15872491665094 39.0 35.0 39.0 32.0 39.0 14-15 37.138748663269794 39.0 36.5 41.0 31.5 41.0 16-17 37.017590779706865 39.0 36.0 41.0 31.0 41.0 18-19 37.09005777426559 39.0 36.5 41.0 31.0 41.0 20-21 37.19886712115493 40.0 37.0 41.0 31.0 41.0 22-23 37.11706857032773 39.0 36.0 41.0 31.0 41.0 24-25 37.02522930820281 39.0 36.0 41.0 31.0 41.0 26-27 36.850567815468324 39.0 36.0 41.0 30.5 41.0 28-29 36.67472783779958 39.0 36.0 41.0 30.0 41.0 30-31 36.427336918915515 39.0 35.5 40.5 30.0 41.0 32-33 36.103622637132794 38.5 35.0 40.0 30.0 41.0 34-35 35.85282403912688 38.0 35.0 40.0 29.0 41.0 36-37 35.579230259640184 38.0 35.0 40.0 27.5 41.0 38-39 35.32209546219413 38.0 35.0 40.0 27.0 41.0 40-41 35.17660220875008 38.0 35.0 40.0 26.0 41.0 42-43 34.93923725781594 38.0 34.0 40.0 25.0 41.0 44-45 34.552035663175445 38.0 34.0 40.0 24.0 41.0 46-47 34.059919914134746 38.0 33.0 40.0 21.5 41.0 48-49 33.63026288372019 38.0 33.0 40.0 16.5 41.0 50-51 32.88304495345034 37.5 32.0 39.5 14.0 40.5 52-53 32.9520305521482 37.5 33.0 39.5 11.0 40.5 54-55 33.11199391001447 38.0 33.0 40.0 8.5 41.0 56-57 32.854172564005786 38.0 32.5 40.0 7.5 41.0 58-59 32.58658129285399 38.0 32.0 40.0 2.0 41.0 60-61 32.223017707743594 37.0 31.0 40.0 2.0 41.0 62-63 31.762158838931875 37.0 31.0 39.5 2.0 41.0 64-65 31.345984600081778 36.0 30.5 39.0 2.0 41.0 66-67 30.866080766025036 36.0 29.5 39.0 2.0 40.0 68-69 30.34509950776876 35.0 29.0 38.5 2.0 40.0 70-71 29.78320005346921 35.0 28.0 38.0 2.0 40.0 72-73 29.265616645436246 34.5 28.0 37.5 2.0 39.5 74-75 28.695889947788892 34.0 26.5 37.0 2.0 39.0 76-77 27.993272118953264 34.0 26.0 36.0 2.0 39.0 78-79 27.240771155721205 33.0 25.0 36.0 2.0 38.0 80-81 26.58713760851104 32.5 23.0 35.0 2.0 37.0 82-83 25.94363372413034 32.0 21.0 35.0 2.0 36.5 84-85 25.289185852676606 32.0 19.0 35.0 2.0 36.0 86-87 24.633394370793233 31.0 15.0 34.5 2.0 36.0 88-89 23.8628259262754 31.0 6.5 34.0 2.0 35.0 90-91 23.054123812669058 30.5 2.0 34.0 2.0 35.0 92-93 22.237121685695413 29.5 2.0 34.0 2.0 35.0 94-95 21.310342065326793 29.0 2.0 34.0 2.0 35.0 96-97 20.122027249638297 28.0 2.0 34.0 2.0 35.0 98-99 18.699920779077814 25.5 2.0 33.5 2.0 35.0 100-101 15.25060595159464 10.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4900084351813305 1101 2 0.5436910862783222 1101 3 0.5459667955132339 1101 4 0.5330855806544434 1101 5 0.4750499338335885 1101 6 0.5281709229454847 1101 7 0.4950392621352009 1101 8 0.6617881757722017 1101 9 0.5100716051917118 1101 10-11 0.6333659958267432 1101 12-13 0.657188433589738 1101 14-15 0.8385668222289766 1101 16-17 0.8206911351647008 1101 18-19 0.7691284963761262 1101 20-21 0.8823354313558269 1101 22-23 0.9350345393226434 1101 24-25 1.0017773872959452 1101 26-27 1.0554686393512398 1101 28-29 1.0494126822794954 1101 30-31 0.8559180157872177 1101 32-33 0.9142134377854845 1101 34-35 0.8377240886132284 1101 36-37 0.7781427680697348 1101 38-39 1.0216938256085015 1101 40-41 0.7330787854015313 1101 42-43 0.9006390059762452 1101 44-45 1.0989753028558553 1101 46-47 1.1208241932834682 1101 48-49 0.9296738064631924 1101 50-51 0.6597714464523037 1101 52-53 0.6943222963427047 1101 54-55 0.6429039369366052 1101 56-57 0.6186526028791377 1101 58-59 0.40748213979906467 1101 60-61 0.5343977715445121 1101 62-63 0.5264724612320073 1101 64-65 0.5852841315708694 1101 66-67 0.45325735070734297 1101 68-69 0.500261323885848 1101 70-71 0.8067275778373997 1101 72-73 0.696223951483546 1101 74-75 0.525695673552999 1101 76-77 0.8641076727495864 1101 78-79 0.8145073106916492 1101 80-81 0.9199814872956047 1101 82-83 0.9020987723348277 1101 84-85 1.382489516387647 1101 86-87 1.4246795267969823 1101 88-89 1.5854559639402943 1101 90-91 1.6511401941920987 1101 92-93 1.3761598535654471 1101 94-95 1.447097769411151 1101 96-97 1.4285012844704674 1101 98-99 1.4300292683378792 1101 100-101 1.3614185383148385 1102 1 0.5274071267153602 1102 2 0.6195137598453577 1102 3 0.7515538150100305 1102 4 0.647958802670523 1102 5 0.3881931815633024 1102 6 0.5543809284787997 1102 7 0.48430626080069317 1102 8 0.41790736986132515 1102 9 0.5524502791493333 1102 10-11 0.7094897467625216 1102 12-13 0.717981650397995 1102 14-15 0.7069349828903739 1102 16-17 0.7641903051676309 1102 18-19 0.6953319921284873 1102 20-21 0.7122511296925751 1102 22-23 0.6883667334443118 1102 24-25 0.6339991755327716 1102 26-27 0.7315297336454165 1102 28-29 0.6366478155672581 1102 30-31 0.6116954463766788 1102 32-33 0.5389489779345666 1102 34-35 0.7092820419380956 1102 36-37 0.7241685468022752 1102 38-39 0.7894573903645394 1102 40-41 0.7805635687885726 1102 42-43 0.8589194473393889 1102 44-45 0.8455495306330931 1102 46-47 0.5619594990804231 1102 48-49 0.38944336021770454 1102 50-51 0.3080520505599793 1102 52-53 0.30840620741908964 1102 54-55 0.20578435226062908 1102 56-57 0.5634130429403257 1102 58-59 0.5212486990543397 1102 60-61 0.44849307473756284 1102 62-63 0.2802806830853406 1102 64-65 0.20864854910822217 1102 66-67 0.08977966298153106 1102 68-69 0.20789078715440823 1102 70-71 -0.03531665612413093 1102 72-73 0.15539948772675416 1102 74-75 0.04151444125146497 1102 76-77 0.1371075425539665 1102 78-79 0.050604859759122434 1102 80-81 0.3842427899027747 1102 82-83 0.5597371865521872 1102 84-85 0.5440175247201644 1102 86-87 0.6795293136016554 1102 88-89 0.7896149002420856 1102 90-91 0.6836077266596305 1102 92-93 1.0076940461898687 1102 94-95 0.8697981892920232 1102 96-97 1.1480273724176087 1102 98-99 1.3578886896183526 1102 100-101 0.9704329574796322 1103 1 0.6531837531554068 1103 2 0.6182290354292732 1103 3 0.6398880960908819 1103 4 0.6882696638570991 1103 5 0.6882500464145096 1103 6 0.7726155907647652 1103 7 0.6372095273704801 1103 8 0.6297394711228819 1103 9 0.843324523583945 1103 10-11 0.769987630126387 1103 12-13 0.8166948198763109 1103 14-15 0.8893237278620418 1103 16-17 0.9757696737571777 1103 18-19 1.0127958197648042 1103 20-21 0.915922071753613 1103 22-23 0.853853980974904 1103 24-25 1.0011776258602723 1103 26-27 1.109291364613597 1103 28-29 0.8120864120584912 1103 30-31 0.9093302897877251 1103 32-33 1.1682006786148378 1103 34-35 1.203406007313724 1103 36-37 1.0168645725810137 1103 38-39 0.9742892178323714 1103 40-41 1.0827581308239118 1103 42-43 0.8823077500288932 1103 44-45 0.922759361934034 1103 46-47 0.8942081880296939 1103 48-49 0.9630728119984227 1103 50-51 0.9738973360425902 1103 52-53 0.9709027849974916 1103 54-55 0.9552841521805959 1103 56-57 1.0141617634811766 1103 58-59 0.8540765670436485 1103 60-61 0.9023798926226121 1103 62-63 1.0085298774999423 1103 64-65 1.0038087184391138 1103 66-67 0.8365099340373234 1103 68-69 1.0466473424080895 1103 70-71 1.1273905320142852 1103 72-73 1.1201011578684934 1103 74-75 0.9610822683823166 1103 76-77 1.2103894869106604 1103 78-79 1.2431239726674441 1103 80-81 1.1883328469782342 1103 82-83 1.4544003149614504 1103 84-85 1.29362262992014 1103 86-87 1.1729372030732357 1103 88-89 1.266826837648626 1103 90-91 1.3450407209099602 1103 92-93 1.2415770730848337 1103 94-95 1.4018425859980752 1103 96-97 1.2580082068566476 1103 98-99 1.3018662806547674 1103 100-101 1.3023973221728777 1104 1 0.7441209892938829 1104 2 0.7255092680965021 1104 3 0.8360822356286768 1104 4 0.7636050611739194 1104 5 0.6477772065608605 1104 6 0.7100612548358143 1104 7 0.6837117008076419 1104 8 0.7231878871719175 1104 9 0.7578349529550579 1104 10-11 0.8262952887560289 1104 12-13 0.6499734263747357 1104 14-15 0.8719362305983864 1104 16-17 0.9282308177043888 1104 18-19 0.9888254660730951 1104 20-21 1.015163714184105 1104 22-23 0.9975525768406897 1104 24-25 1.1252261607447167 1104 26-27 1.0492998081824112 1104 28-29 0.9162597241265402 1104 30-31 1.025254235123434 1104 32-33 0.9399710135430652 1104 34-35 1.063589889479033 1104 36-37 1.0186189585459218 1104 38-39 1.0549550088696762 1104 40-41 1.0678911952139387 1104 42-43 0.9958049511421905 1104 44-45 1.001428405308964 1104 46-47 1.0632484357077132 1104 48-49 0.9281586549480423 1104 50-51 0.6880903497712083 1104 52-53 0.784365586385988 1104 54-55 0.9407033597360268 1104 56-57 1.0564953157218469 1104 58-59 0.9413205236374438 1104 60-61 1.1946430817898275 1104 62-63 1.2456643804239214 1104 64-65 1.5424630637498034 1104 66-67 1.4701043925543793 1104 68-69 1.4748644984890227 1104 70-71 1.3576294537392748 1104 72-73 1.5181575734171702 1104 74-75 1.30758600544333 1104 76-77 1.135355868997781 1104 78-79 1.4551638763482124 1104 80-81 1.380298947613067 1104 82-83 1.6293354245714795 1104 84-85 1.5678791267772567 1104 86-87 1.265444317561773 1104 88-89 1.40814716163149 1104 90-91 1.3739396169915175 1104 92-93 1.386729839135434 1104 94-95 1.4614917088050916 1104 96-97 1.54863115460034 1104 98-99 1.3071216204302267 1104 100-101 1.007666734563033 1105 1 0.535251444886331 1105 2 0.6732342972627947 1105 3 0.4233796919290924 1105 4 0.44639545275009596 1105 5 0.4647744245452472 1105 6 0.36454588395025667 1105 7 0.47821148642292144 1105 8 0.534906255454942 1105 9 0.6658260185966682 1105 10-11 0.5980448674651342 1105 12-13 0.7010842383443787 1105 14-15 0.7150465041133955 1105 16-17 0.606884581402575 1105 18-19 0.5846404769917513 1105 20-21 0.7384184730262291 1105 22-23 0.7990667312176214 1105 24-25 0.8131248913877727 1105 26-27 1.041400977825532 1105 28-29 0.695332285874322 1105 30-31 0.8113491427385782 1105 32-33 0.8952403904313968 1105 34-35 0.9146413717249828 1105 36-37 0.7885432757555293 1105 38-39 0.69495869828107 1105 40-41 0.8452691205681688 1105 42-43 0.9750515135081983 1105 44-45 0.871136343526274 1105 46-47 0.905203026033476 1105 48-49 0.9073279391025082 1105 50-51 0.9331295871599039 1105 52-53 0.790342432836681 1105 54-55 0.8529178042801888 1105 56-57 1.0313454957052954 1105 58-59 1.0566265952120233 1105 60-61 1.1765530003236293 1105 62-63 1.180582664947341 1105 64-65 1.1401920814689497 1105 66-67 0.9943802209269528 1105 68-69 1.0391091164355153 1105 70-71 1.1864422609824885 1105 72-73 1.2525384004436049 1105 74-75 1.0208624021846795 1105 76-77 1.2235063770397048 1105 78-79 1.0058494088698744 1105 80-81 1.1906382737470587 1105 82-83 1.3491106696181951 1105 84-85 1.1130704277434837 1105 86-87 1.4091807938747536 1105 88-89 1.3456315813469075 1105 90-91 0.9997126483914052 1105 92-93 1.295605864941372 1105 94-95 1.3929494251591557 1105 96-97 1.5985354293318608 1105 98-99 1.6051108912230525 1105 100-101 1.1949153310909981 1106 1 0.3298607397395159 1106 2 0.35821871551183193 1106 3 0.01481069671007873 1106 4 0.3636050611739208 1106 5 0.2855668678799361 1106 6 -0.045702262692408624 1106 7 0.3371579277832808 1106 8 0.38572502323846436 1106 9 0.33775176816599384 1106 10-11 0.09227865179821748 1106 12-13 0.21999719345732416 1106 14-15 0.16241454313551884 1106 16-17 0.10678102566635772 1106 18-19 -0.06037239488946966 1106 20-21 0.32043703563390125 1106 22-23 0.4483844247313584 1106 24-25 0.343437687779776 1106 26-27 0.15856897039274997 1106 28-29 0.1063072582917215 1106 30-31 0.2588400937092956 1106 32-33 0.2592223504414619 1106 34-35 0.2836433654148607 1106 36-37 -0.006381041454076808 1106 38-39 0.08473813424104293 1106 40-41 0.18784069016343352 1106 42-43 -0.3532443655541826 1106 44-45 -0.07380926551694955 1106 46-47 -0.18773195644914864 1106 48-49 0.10850624853092228 1106 50-51 0.08424305327685744 1106 52-53 0.3835188840686996 1106 54-55 -0.05828653925386362 1106 56-57 -0.5473965440523685 1106 58-59 -0.5410472719656383 1106 60-61 0.10010951078566421 1106 62-63 0.19795196212327326 1106 64-65 0.18182729429050326 1106 66-67 -0.130801727469386 1106 68-69 -0.394193730863325 1106 70-71 -0.18179419450790135 1106 72-73 0.30655626622762355 1106 74-75 0.14415760377963593 1106 76-77 0.05355254160621925 1106 78-79 0.39895472602141524 1106 80-81 0.2385134455334459 1106 82-83 0.6124756503587641 1106 84-85 0.5554905349769008 1106 86-87 0.47180201215476103 1106 88-89 0.24636760132251823 1106 90-91 0.3191119283403019 1106 92-93 0.212784503425393 1106 94-95 0.3889426891972505 1106 96-97 0.3389163595914262 1106 98-99 0.4262986851955297 1106 100-101 -0.7519886427394038 1107 1 0.5789354257937127 1107 2 0.4910012667345711 1107 3 0.35292481876339465 1107 4 0.4986237877656805 1107 5 0.48075303223331645 1107 6 0.465412520299445 1107 7 0.40275607715538797 1107 8 0.38989995163678515 1107 9 0.4879280184413801 1107 10-11 0.5042801939881301 1107 12-13 0.5430474247858115 1107 14-15 0.6063449242767476 1107 16-17 0.5097993116324275 1107 18-19 0.3837011896005009 1107 20-21 0.5331270554620531 1107 22-23 0.490910311490552 1107 24-25 0.5726952334946915 1107 26-27 0.5521854057309241 1107 28-29 0.47993581083633785 1107 30-31 0.6515367229227778 1107 32-33 0.6018198388750378 1107 34-35 0.6364193072522681 1107 36-37 0.7982069735271935 1107 38-39 0.8627123576382658 1107 40-41 0.761436195781414 1107 42-43 0.6694203182968792 1107 44-45 0.562823252658859 1107 46-47 0.5425555518125762 1107 48-49 0.7614749641070446 1107 50-51 0.7496043663414085 1107 52-53 0.7263642244520412 1107 54-55 0.44058986524051136 1107 56-57 0.47885373133868825 1107 58-59 0.3976506791249079 1107 60-61 0.34435934555103387 1107 62-63 0.6302773641942103 1107 64-65 0.6985435313471235 1107 66-67 0.38714275369386897 1107 68-69 0.4166787079998606 1107 70-71 0.3823939526610758 1107 72-73 0.21640975818620944 1107 74-75 0.321806865731606 1107 76-77 0.4501101534149896 1107 78-79 0.31867823340189716 1107 80-81 0.25013494806704273 1107 82-83 0.3855974833616287 1107 84-85 0.6474160692355468 1107 86-87 0.5783287801653358 1107 88-89 0.7981851822874866 1107 90-91 0.719723296483064 1107 92-93 0.6999292489640574 1107 94-95 1.1229149297788723 1107 96-97 1.2762869257954357 1107 98-99 1.0904152306901302 1107 100-101 0.903073044856983 1108 1 0.3075591088067675 1108 2 0.25227222476600275 1108 3 0.2882393204960394 1108 4 0.2819851234792168 1108 5 0.3827814546492263 1108 6 0.2337278271005232 1108 7 0.3778304584777956 1108 8 0.25941936078656624 1108 9 0.16753003461275995 1108 10-11 0.26957386895049495 1108 12-13 0.22813353210493403 1108 14-15 0.37078452664294304 1108 16-17 0.3835173276637889 1108 18-19 0.4023910282367851 1108 20-21 0.39944106715304883 1108 22-23 0.35792263253784284 1108 24-25 0.47058630423570946 1108 26-27 0.4350120709974732 1108 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-0.8962553688640895 2316 86-87 -1.3138278562309473 2316 88-89 -1.3492072039420542 2316 90-91 -1.0607497423553731 2316 92-93 -1.3189640864215058 2316 94-95 -1.2535767843455936 2316 96-97 -1.348110231867075 2316 98-99 -1.3956526980975141 2316 100-101 -1.114680020660435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6203.0 3 1311.0 4 1048.0 5 1027.0 6 1191.0 7 1402.0 8 1799.0 9 2332.0 10 3127.0 11 4220.0 12 5831.0 13 7767.0 14 9447.0 15 12099.0 16 14415.0 17 17643.0 18 14917.0 19 11323.0 20 11191.0 21 11763.0 22 12525.0 23 14138.0 24 15870.0 25 17624.0 26 20235.0 27 23005.0 28 26502.0 29 30883.0 30 35787.0 31 42302.0 32 50272.0 33 61248.0 34 75737.0 35 96014.0 36 121493.0 37 144749.0 38 84094.0 39 4874.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.53167117362866 21.25506910127961 15.504802721115233 21.7084570039765 2 15.251562277212633 22.106701935699572 40.86396019450413 21.77777559258367 3 18.064379729284568 26.54427274112898 31.734476638133213 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48-49 19.676682741930094 28.806750824242254 31.1477069649208 20.368859468906848 50-51 19.90865272849965 29.051194478861998 30.388326658157432 20.651826134480917 52-53 19.849958272593497 29.254745570947893 30.500227559354652 20.39506859710395 54-55 20.129341931881687 28.97611184862728 30.225984674190148 20.668561545300886 56-57 20.124493462004427 29.141545576746804 30.006119003560816 20.72784195768795 58-59 19.880801595996445 28.967050380150113 30.178153741956 20.973994281897443 60-61 20.105515064207008 28.874599366654035 30.130330862566478 20.889554706572476 62-63 19.92783377982619 28.738462324800086 30.313400142622154 21.02030375275157 64-65 19.97177963740127 28.67384446166783 30.117692968156007 21.23668293277489 66-67 19.978375562419675 28.821591017994976 29.823244796005376 21.376788623579976 68-69 20.158277992065457 28.43204328217952 30.00061927173644 21.409059454018582 70-71 19.971647019152027 28.274872514813055 30.17036366335456 21.58311680268036 72-73 20.140256328837044 27.96814617251715 30.24044222966962 21.65115526897619 74-75 20.13396394465668 28.160495235024293 29.76119086052689 21.944349959792138 76-77 19.890345649582837 28.149859307226325 30.103240234447 21.85655480874384 78-79 20.035788461784193 28.103771795232202 29.741725454039692 22.11871428894391 80-81 20.13576395237056 27.892405200156485 29.621989985786655 22.349840861686296 82-83 20.27804276061164 27.943312185491585 29.736676687686725 22.041968366210043 84-85 20.378106450132154 27.59635487571251 29.656443792211007 22.369094881944328 86-87 20.199444621925146 27.631287936500136 29.69754011746492 22.471727324109796 88-89 20.801937249781734 27.350134498462314 29.631250049158798 22.216678202597155 90-91 20.90298183505786 27.217522588802918 29.45184636025177 22.427649215887456 92-93 20.977967058976198 26.95305606718172 29.569501608120085 22.499475265721998 94-95 21.020897752913935 26.89920803858047 29.57889872627434 22.500995482231257 96-97 21.36781087627581 26.630981708345274 29.268810681103215 22.7323967342757 98-99 21.415928159548013 26.428814041703756 29.122241135825504 23.033016662922723 100-101 22.120913607972074 26.021087500135316 29.305159588130458 22.552839303762156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2239.0 1 1329.5 2 442.0 3 436.5 4 339.0 5 322.0 6 523.5 7 897.5 8 1287.0 9 1402.5 10 1140.5 11 892.5 12 890.5 13 1005.5 14 1156.5 15 1288.0 16 1420.0 17 1586.5 18 1832.5 19 2120.5 20 2485.0 21 2901.0 22 3467.5 23 4063.0 24 4775.5 25 6095.5 26 8106.5 27 10498.0 28 12215.5 29 14215.5 30 17259.0 31 20271.5 32 23521.5 33 27198.0 34 31418.5 35 35446.0 36 39227.0 37 42393.0 38 42437.0 39 40654.5 40 39248.5 41 38097.5 42 37526.0 43 38126.0 44 38565.0 45 38213.5 46 37459.0 47 36896.5 48 36678.5 49 35334.5 50 33430.0 51 31172.0 52 28363.0 53 25506.5 54 22477.0 55 19321.0 56 16282.5 57 13353.0 58 10624.5 59 8135.0 60 6004.5 61 4400.0 62 3167.0 63 2215.0 64 1519.0 65 1126.5 66 837.5 67 600.5 68 487.0 69 408.0 70 335.0 71 259.5 72 231.5 73 204.5 74 155.5 75 127.0 76 111.0 77 89.0 78 58.5 79 44.0 80 33.0 81 25.5 82 24.0 83 16.5 84 9.0 85 6.5 86 7.0 87 6.0 88 4.0 89 2.0 90 2.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1908772095363905 2 0.045507800213876834 3 0.04649069006730829 4 0.05032396049569102 5 0.0574990564257407 6 0.06418270742907467 7 0.03449943385544443 8 0.03282852110461094 9 0.03882414921054287 10-11 0.041821963263508836 12-13 0.03695665848902309 14-15 0.03371312197269925 16-17 0.0315999087878216 18-19 0.040101906020003776 20-21 0.04968508209096056 22-23 0.047129568472038745 24-25 0.06570618670189345 26-27 0.05248631817324023 28-29 0.043836887463043345 30-31 0.05204401773919608 32-33 0.04894791470088696 34-35 0.04860390325218594 36-37 0.045606089199219976 38-39 0.025751714159904383 40-41 0.023982512423727745 42-43 0.02132870981946279 44-45 0.0035875479650248473 46-47 0.010172909983015663 48-49 0.010811788387746116 50-51 0.008010552305466441 52-53 0.008944297666226333 54-55 0.010271198968358809 56-57 0.008403708246839026 58-59 0.011057510851103982 60-61 0.011057510851103982 62-63 0.0033909699943385544 64-65 0.005651616657230924 66-67 0.003980703906397433 68-69 0.008256274768824307 70-71 0.022704755614266842 72-73 0.019559508083286156 74-75 0.019068063156570423 76-77 0.011843822733849154 78-79 0.004029848399069007 80-81 0.005307605208529911 82-83 0.004570437818456313 84-85 0.004816160281814179 86-87 0.00815798578348116 88-89 0.029093539661571365 90-91 0.036022913128263195 92-93 0.022262455180222684 94-95 0.030764452412404858 96-97 0.03533489023086117 98-99 0.020591542429389195 100-101 0.12394241051770774 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1017408.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.6619511488705 #Duplication Level Percentage of deduplicated Percentage of total 1 77.0978806597542 46.76907870263456 2 13.409233158262479 16.268604935806657 3 4.618367378796433 8.404775289602588 4 1.9293274873192834 4.681470791437416 5 1.00428406001752 3.0460915294186064 6 0.5603206627458418 2.0394086802714595 7 0.31902672686998135 1.35469486043996 8 0.20541678593366952 0.9968786426772998 9 0.14137437922494392 0.7718441117620896 >10 0.622194597973922 6.812912694683688 >50 0.05555399951277581 2.3291678528172173 >100 0.034909308762616734 3.7502397217956625 >500 9.742129968517396E-4 0.3405010247500615 >1k 8.118441640431163E-4 1.0759714186779497 >5k 3.2473766561724656E-4 1.3583597432247896 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7730 0.7597738567025225 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6061 0.595729540164811 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4214 0.4141897842360194 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3019 0.2967344467509593 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1487 0.14615572120525885 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1110 0.10910077373089261 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1094 0.10752814996540228 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 9.82889853431465E-5 0.0 8 0.0 0.0 0.0 9.82889853431465E-5 0.0 9 0.0 0.0 0.0 9.82889853431465E-5 0.0 10-11 0.0 0.0 0.0 1.96577970686293E-4 0.0 12-13 0.0 0.0 0.0 2.948669560294395E-4 0.0 14-15 0.0 0.0 0.0 3.4401144870101273E-4 0.0 16-17 0.0 0.0 0.0 5.89733912058879E-4 0.0 18-19 0.0 0.0 0.0 6.880228974020256E-4 0.0 20-21 0.0 0.0 0.0 9.337453607598917E-4 0.0 22-23 0.0 0.0 0.0 0.002309791155563943 0.0 24-25 0.0 0.0 0.0 0.0058481946279172175 0.0 26-27 0.0 0.0 0.0 0.012433556645908034 0.0 28-29 0.0 0.0 0.0 0.02693118198402214 0.0 30-31 0.0 0.0 0.0 0.07071892495439391 0.0 32-33 0.0 0.0 0.0 0.14669631062464616 0.0 34-35 0.0 0.0 0.0 0.27255535635654526 0.0 36-37 0.0 0.0 0.0 0.45409511228533683 0.0 38-39 0.0 0.0 0.0 0.6876788859533245 0.0 40-41 0.0 0.0 0.0 0.9262262533811412 0.0 42-43 0.0 0.0 0.0 1.163250141536139 0.0 44-45 0.0 0.0 0.0 1.4620486569793043 0.0 46-47 0.0 0.0 0.0 1.788318943825879 0.0 48-49 0.0 0.0 0.0 2.1254010190602 0.0 50-51 0.0 0.0 0.0 2.4708868025413597 0.0 52-53 0.0 0.0 0.0 2.796763933446562 0.0 54-55 0.0 0.0 0.0 3.1717364125306657 0.0 56-57 0.0 0.0 0.0 3.60823779643958 0.0 58-59 0.0 0.0 0.0 4.0685742592942065 0.0 60-61 0.0 0.0 0.0 4.538493898219789 0.0 62-63 0.0 0.0 0.0 4.991409542681009 0.0 64-65 0.0 0.0 0.0 5.482412168962697 0.0 66-67 0.0 0.0 0.0 6.051849405548216 0.0 68-69 0.0 0.0 0.0 6.674510127697049 0.0 70-71 0.0 0.0 0.0 7.32926220356042 0.0 72-73 0.0 0.0 0.0 7.965830817135309 0.0 74-75 0.0 0.0 0.0 8.616749622570296 4.914449267157325E-5 76-77 0.0 0.0 0.0 9.332195146883059 9.82889853431465E-5 78-79 0.0 0.0 0.0 10.094475372711832 9.82889853431465E-5 80-81 0.0 0.0 0.0 10.901870242813109 9.82889853431465E-5 82-83 0.0 0.0 0.0 11.72779258665157 9.82889853431465E-5 84-85 0.0 0.0 0.0 12.539315594137259 9.82889853431465E-5 86-87 0.0 0.0 0.0 13.394822922564005 9.82889853431465E-5 88-89 0.0 0.0 0.0 14.278244322828208 9.82889853431465E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3145 0.0 51.071842 1 GTATCAA 5535 0.0 39.696808 1 GTGGTAT 1320 0.0 32.135746 1 TATCAAC 7000 0.0 31.442778 2 ATCAACG 7115 0.0 31.135008 3 TCAACGC 7170 0.0 30.763573 4 CAACGCA 7330 0.0 29.959408 5 AACGCAG 7400 0.0 29.670164 6 TACACCG 85 6.380702E-4 27.960587 5 ACGCAGA 8485 0.0 25.925793 7 CGCAGAG 8515 0.0 25.722853 8 TGGTATC 1645 0.0 25.719471 2 CATTCCG 250 1.4551915E-11 24.70257 9 GCAGAGT 8930 0.0 23.885464 9 GTACATG 4645 0.0 22.676647 1 GAGTACT 5935 0.0 22.091599 12-13 TACATGG 4965 0.0 21.447008 2 TCGCCGG 100 5.3493386E-7 21.391954 94-95 GGACGTG 400 0.0 21.38564 6 CATGGGT 1385 0.0 21.280403 4 >>END_MODULE