FastQCFastQC Report
Thu 26 May 2016
SRR938670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938670_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017408
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72730.7148557904007046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62000.6093917091275084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42280.4155658300308234No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32790.3222895829401774No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT24080.23667987670629675No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT15470.15205306032584764RNA PCR Primer, Index 33 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15340.15077530351638674No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.11293404415927534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32000.054.1109541
GTATCAA54300.038.546651
TCAACGC69300.030.316634
TATCAAC68950.030.2170642
ATCAACG69250.030.201243
CAACGCA71400.029.3583935
AACGCAG71500.029.294286
CATAACG650.005803212529.2724482
CGCAGAG82100.025.3963688
ACGCAGA82550.025.2579277
GTGGTAT13450.024.7576561
CCTGTAC3850.023.4576633
GCAGAGT87750.023.382289
GAGTACT58500.022.48920812-13
GTACATG45300.022.3673841
CGTCAGA1302.9120134E-421.9554161
GTGTAGC7000.021.7463171
GTACTTT60700.021.5176214-15
CAGAGTA86350.021.47874610-11
ATGGGCG2457.456038E-921.3411835
Produced by FastQC (version 0.11.5)