FastQCFastQC Report
Thu 26 May 2016
SRR938669_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938669_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018460
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78520.770967931975728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62980.6183846199163443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44240.43438132081770514No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31520.30948687233666516No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16340.1604383088191976No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11030.10830076782593329No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.10604245625748679No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31650.053.0084341
GTATCAA58300.042.511931
TAGCGCG459.6213474E-442.2250829
TATCAAC72550.034.064522
ATCAACG73650.033.684813
TCAACGC73400.033.6700444
CAACGCA74950.032.9737325
AACGCAG75800.032.6618586
GTGGTAT12950.031.2842081
ACGCAGA85700.028.7153827
CGCAGAG86300.028.295548
ACGGGAG700.00836827627.1580353
GCAGAGT91250.025.9250459
TGGTATC15600.025.286572
CGACGAG951.1823431E-825.0195194-95
GAGTACT61550.023.84574112-13
GTATAGC6050.022.8464451
GTACTTT64750.022.41161314-15
GTACATG47150.022.3401761
AGTACTT62300.022.30068612-13
Produced by FastQC (version 0.11.5)