FastQCFastQC Report
Thu 26 May 2016
SRR938669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938669_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018460
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73240.7191249533609567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63780.6262396166761581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43770.4297665102213145No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32760.3216621173143766No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT24890.244388586689708No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15550.15268149951888144No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT15240.1496376882744536RNA PCR Primer, Index 33 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.10486420674351471No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32200.051.2461051
GTATCAA55600.038.8300821
CGCTACG250.001616640737.99745618-19
CATTCCG2600.032.8816079
ATCAACG70100.030.6549723
TATCAAC71250.030.3010882
TCAACGC71100.030.1569544
CAACGCA72750.029.472985
AACGCAG73700.029.128956
CGCAGAG84250.025.3685998
ACGCAGA84150.025.2858647
TAGGTCG950.001220105225.0222825
TAGGACG4350.024.04444
GTGGTAT14700.023.6152611
GCAGAGT89900.023.4572539
GTACATG46000.023.2601181
CGTCAGA1452.3135855E-522.9571132
TTAGGCA5450.022.6807484
CTCCGAC2200.022.6683894-95
GGTCGTT1050.00219721922.616987
Produced by FastQC (version 0.11.5)