FastQCFastQC Report
Thu 26 May 2016
SRR938668_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938668_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010874
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71800.7102764538409337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59200.5856318393785971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40250.39817029619913064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30170.29845460462926143No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15430.1526401905677661No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.1024855718912545No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.051.8422361
GTATCAA57400.043.1056061
TATCAAC72100.034.2015882
ATCAACG72850.033.6538163
TCAACGC73950.033.153224
CAACGCA76000.032.1323285
GTGGTAT13850.032.0118981
AACGCAG76450.031.938456
CCGCGAC300.003951549531.6668490-91
ACGCAGA85050.028.594397
TAGGACG3350.028.3660834
CGCAGAG85650.028.2831678
GCAGAGT89250.026.6633539
TGGTATC16800.026.3019472
GTACATG44600.026.0815411
AGGACGT4700.025.2717235
TACATGG46800.024.7718332
CGTATAG1351.3050871E-524.7196641
ACATGGG46600.023.8585533
GACGCTC1650.023.05152994-95
Produced by FastQC (version 0.11.5)