##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938668_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1010874 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.108482362787054 31.0 31.0 34.0 28.0 34.0 2 31.271416615720653 31.0 31.0 34.0 28.0 34.0 3 31.29585190637013 31.0 31.0 34.0 28.0 34.0 4 34.978987490033376 37.0 35.0 37.0 32.0 37.0 5 34.987797687941324 37.0 35.0 37.0 32.0 37.0 6 34.980734493121794 37.0 35.0 37.0 32.0 37.0 7 34.91962895474609 37.0 35.0 37.0 32.0 37.0 8 34.89139892805632 37.0 35.0 37.0 32.0 37.0 9 36.38754681592365 39.0 37.0 39.0 32.0 39.0 10-11 36.2484147381375 39.0 35.0 39.0 32.0 39.0 12-13 36.10543945140542 38.5 35.0 39.0 31.5 39.0 14-15 37.22572348284751 39.0 37.0 41.0 32.0 41.0 16-17 37.17390149514183 39.0 36.5 41.0 32.0 41.0 18-19 37.23618423265412 39.5 37.0 41.0 32.0 41.0 20-21 37.22625272783749 40.0 37.0 41.0 31.5 41.0 22-23 37.07627854707906 39.0 36.0 41.0 31.0 41.0 24-25 36.96626087919958 39.0 36.0 41.0 31.0 41.0 26-27 36.77487303066455 39.0 36.0 41.0 30.5 41.0 28-29 36.55692252446892 39.0 36.0 41.0 30.0 41.0 30-31 36.273864497454674 39.0 35.0 40.0 30.0 41.0 32-33 35.93440329853176 38.0 35.0 40.0 29.5 41.0 34-35 35.63509398797476 38.0 35.0 40.0 28.0 41.0 36-37 35.388459392565245 38.0 35.0 40.0 27.0 41.0 38-39 35.152114407928195 38.0 34.0 40.0 26.0 41.0 40-41 34.886173252057134 38.0 34.0 40.0 25.0 41.0 42-43 34.461940855141194 38.0 33.5 40.0 24.0 41.0 44-45 33.92178451518191 38.0 33.0 40.0 22.0 41.0 46-47 33.34282610889191 38.0 33.0 40.0 17.0 41.0 48-49 32.8123564361137 37.5 32.0 40.0 11.5 41.0 50-51 32.09993629275261 36.5 30.5 39.5 9.0 40.5 52-53 32.14386362692086 37.0 31.0 39.0 7.5 40.0 54-55 32.19267089666961 37.0 31.0 40.0 4.0 41.0 56-57 31.85090129927172 37.0 31.0 40.0 2.0 41.0 58-59 31.482223798416022 37.0 30.5 39.5 2.0 41.0 60-61 31.037739619378875 36.0 29.5 39.0 2.0 40.5 62-63 30.405744929635148 36.0 28.0 39.0 2.0 40.0 64-65 29.846805833367956 35.0 28.0 39.0 2.0 40.0 66-67 29.246154317946647 34.5 26.5 38.0 2.0 40.0 68-69 28.469384413883432 34.0 26.0 38.0 2.0 40.0 70-71 27.722687001545196 34.0 24.5 37.0 2.0 39.0 72-73 26.895199104932956 33.0 22.0 36.5 2.0 39.0 74-75 26.022680373617284 32.0 19.0 36.0 2.0 38.5 76-77 25.11871113511674 31.0 14.5 35.0 2.0 38.0 78-79 24.158873410533854 30.5 7.5 35.0 2.0 37.0 80-81 23.20296594827842 30.0 2.0 34.0 2.0 36.0 82-83 22.208157495395074 29.0 2.0 34.0 2.0 36.0 84-85 21.214533660970606 28.5 2.0 34.0 2.0 35.0 86-87 20.198492591559386 26.5 2.0 33.5 2.0 35.0 88-89 19.093673395497362 25.5 2.0 32.5 2.0 35.0 90-91 17.90888775455695 23.5 2.0 32.0 2.0 34.0 92-93 16.70037512093495 19.0 2.0 31.5 2.0 34.0 94-95 15.383873756768896 4.5 2.0 31.0 2.0 34.0 96-97 13.868369846291426 2.0 2.0 30.5 2.0 34.0 98-99 12.07975623074686 2.0 2.0 29.5 2.0 34.0 100-101 8.505647093505225 2.0 2.0 13.0 2.0 29.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7504490880096775 1101 2 0.5614301214388888 1101 3 0.5639450686935135 1101 4 0.4725802102162575 1101 5 0.645179654453834 1101 6 0.7021016001012441 1101 7 0.6098879400243291 1101 8 0.6202317360360468 1101 9 0.5970788534647085 1101 10-11 0.7515041688725717 1101 12-13 0.5921602059737765 1101 14-15 0.9702349444075438 1101 16-17 1.035799079648946 1101 18-19 0.9356305212778935 1101 20-21 0.9973233140382334 1101 22-23 0.9800720001230587 1101 24-25 1.0514660563744727 1101 26-27 0.9445182819645837 1101 28-29 1.049272191634543 1101 30-31 1.0629165872572912 1101 32-33 1.1154249521737256 1101 34-35 1.0005884167337982 1101 36-37 1.0941058047359604 1101 38-39 1.0070919996895924 1101 40-41 0.9163076688064109 1101 42-43 1.0234270006927275 1101 44-45 1.3950003085439064 1101 46-47 1.6058981106648957 1101 48-49 1.2660908907049873 1101 50-51 1.4242517820584126 1101 52-53 1.410162541344235 1101 54-55 1.470583680319919 1101 56-57 1.4168137732793262 1101 58-59 1.687302900148996 1101 60-61 1.7357329461663262 1101 62-63 1.511996006032284 1101 64-65 2.0324342240884654 1101 66-67 2.457025284609365 1101 68-69 2.407459915793222 1101 70-71 2.6144346525751807 1101 72-73 2.811727717311438 1101 74-75 2.768560740987443 1101 76-77 2.815917047222772 1101 78-79 3.0443334297585274 1101 80-81 3.283609610744225 1101 82-83 3.7518702904512793 1101 84-85 3.50064280039998 1101 86-87 3.6664836279114468 1101 88-89 4.0159255594028345 1101 90-91 4.037808060283531 1101 92-93 4.363907219748363 1101 94-95 4.532798500705699 1101 96-97 4.226248346923791 1101 98-99 4.253311944941734 1101 100-101 3.5926621228944224 1102 1 0.6133400829915274 1102 2 0.4722059048995888 1102 3 0.4173939429927067 1102 4 0.26825742499655547 1102 5 0.4111186012896937 1102 6 0.4398953794791822 1102 7 0.5528504715716025 1102 8 0.594364443424432 1102 9 0.6371087388061838 1102 10-11 0.6245667692502579 1102 12-13 0.6942009982883093 1102 14-15 0.7765226319998391 1102 16-17 0.7673428222584988 1102 18-19 0.8347539630338332 1102 20-21 0.7824494737130223 1102 22-23 0.7855543130602314 1102 24-25 0.6886533045110568 1102 26-27 0.7929754806256284 1102 28-29 0.5847004403417628 1102 30-31 0.6970388230102742 1102 32-33 0.6906407090435351 1102 34-35 0.7982464178280182 1102 36-37 1.0106074549009776 1102 38-39 1.0180272108577668 1102 40-41 1.3665050414848494 1102 42-43 1.4621749931390227 1102 44-45 1.145135910339384 1102 46-47 1.3125458342607814 1102 48-49 1.1783727071219232 1102 50-51 1.3691103855658255 1102 52-53 1.3115667994171005 1102 54-55 1.3365061549203148 1102 56-57 1.7171879283418967 1102 58-59 1.7261214878901292 1102 60-61 1.6887729560496645 1102 62-63 1.7532677802685335 1102 64-65 1.741025083174378 1102 66-67 2.0253621182927333 1102 68-69 2.0449430758739346 1102 70-71 1.6966869954565276 1102 72-73 2.1095216143482 1102 74-75 2.084977676798669 1102 76-77 2.3110606792330337 1102 78-79 2.6330976002932296 1102 80-81 2.459974306037285 1102 82-83 2.882383341756409 1102 84-85 2.920667744070819 1102 86-87 3.1376725115056843 1102 88-89 3.3959541504972393 1102 90-91 3.497642278999521 1102 92-93 3.5169395818080993 1102 94-95 3.8300064567954237 1102 96-97 3.743207101622792 1102 98-99 2.8725856370168206 1102 100-101 2.5803002984766845 1103 1 0.41759939437158167 1103 2 0.3831543991298183 1103 3 0.40075073068386047 1103 4 0.32831934450120315 1103 5 0.3305481913075212 1103 6 0.2584337038998612 1103 7 0.47049753039513575 1103 8 0.19048040176949144 1103 9 0.4976772724397307 1103 10-11 0.4036361003319797 1103 12-13 0.5494313200820997 1103 14-15 0.5382169748087264 1103 16-17 0.525622210568848 1103 18-19 0.7007263807090354 1103 20-21 0.7018790637308499 1103 22-23 0.7153882562068716 1103 24-25 0.7074636994824388 1103 26-27 0.6728047329007012 1103 28-29 0.7096089683462736 1103 30-31 0.5815026563905121 1103 32-33 0.8962697549202545 1103 34-35 0.7234833068419988 1103 36-37 0.42484894096908477 1103 38-39 0.6188434225098902 1103 40-41 0.6459684057779427 1103 42-43 0.824957618150755 1103 44-45 0.49742974653414507 1103 46-47 0.5411976777733045 1103 48-49 0.6297096055176397 1103 50-51 0.703785308166438 1103 52-53 0.7458289253200974 1103 54-55 0.8346485697810024 1103 56-57 0.9732156983015479 1103 58-59 1.0070108771386437 1103 60-61 1.1129872350627075 1103 62-63 1.3325622731853137 1103 64-65 1.2044175214894146 1103 66-67 1.3763988009083619 1103 68-69 1.4164225767652034 1103 70-71 1.5199818636430358 1103 72-73 1.8713942102765238 1103 74-75 1.8889461541417596 1103 76-77 1.9258463064025584 1103 78-79 2.1851475111666687 1103 80-81 2.3290989894034553 1103 82-83 2.5577281208163605 1103 84-85 2.7410730353739403 1103 86-87 2.860366948132011 1103 88-89 3.1331996707148946 1103 90-91 3.247290463632222 1103 92-93 3.0724138289232137 1103 94-95 3.376267832158966 1103 96-97 3.273735293760888 1103 98-99 3.5674444417827456 1103 100-101 2.8445277119301053 1104 1 0.6135046243325419 1104 2 0.5510917249618785 1104 3 0.6014334329145505 1104 4 0.4569863431372383 1104 5 0.5047073654666931 1104 6 0.5379972775810771 1104 7 0.42836319414315227 1104 8 0.5466474545309694 1104 9 0.674976753144783 1104 10-11 0.7700565735635934 1104 12-13 0.7751905969249648 1104 14-15 0.7592869265288371 1104 16-17 0.9186620912248245 1104 18-19 0.7172949514571698 1104 20-21 0.8018477225230356 1104 22-23 0.922229402676507 1104 24-25 0.8383271600672586 1104 26-27 0.8771736354120208 1104 28-29 0.748401445219244 1104 30-31 0.6416530323303675 1104 32-33 0.8787408442024969 1104 34-35 0.8861467527871767 1104 36-37 0.6415764858700044 1104 38-39 0.6780612944212621 1104 40-41 0.4994544450224936 1104 42-43 0.7877435422369885 1104 44-45 0.6195321023826352 1104 46-47 0.7405648740445301 1104 48-49 0.8443772907735649 1104 50-51 0.7242493642342112 1104 52-53 0.7026698140495284 1104 54-55 1.0128357077204555 1104 56-57 1.020678555361929 1104 58-59 1.1984139013590074 1104 60-61 1.4485778570310295 1104 62-63 1.46847022488274 1104 64-65 1.4759901181394106 1104 66-67 1.7354520283826425 1104 68-69 1.8685488818326839 1104 70-71 1.6789988012977446 1104 72-73 1.9901351184911142 1104 74-75 2.0312666819112053 1104 76-77 2.0456202373220016 1104 78-79 2.24342256944173 1104 80-81 2.420660678488364 1104 82-83 2.5532125125855814 1104 84-85 2.7090323206706906 1104 86-87 2.9201018468762 1104 88-89 3.038482210672356 1104 90-91 2.8903378187538813 1104 92-93 2.875927652560872 1104 94-95 3.1931315669793605 1104 96-97 3.475222145402542 1104 98-99 3.111258728631089 1104 100-101 2.80170830223763 1105 1 0.5710154679193344 1105 2 0.31530219803311255 1105 3 0.4602455069164968 1105 4 0.28402006890514286 1105 5 0.4451213818300772 1105 6 0.4602016554816615 1105 7 0.4433482091281675 1105 8 0.5493030268067969 1105 9 0.3861769453576329 1105 10-11 0.6072826386377557 1105 12-13 0.6210030123576331 1105 14-15 0.579504683455454 1105 16-17 0.6251265128791275 1105 18-19 0.8164742531681171 1105 20-21 0.709516003609032 1105 22-23 0.6404668614202933 1105 24-25 0.7164300698157362 1105 26-27 0.565643393502036 1105 28-29 0.6889545629875471 1105 30-31 0.6648829669527032 1105 32-33 0.7039409605418498 1105 34-35 0.5402711094431822 1105 36-37 0.7926911939467374 1105 38-39 0.7640196398732826 1105 40-41 0.865348045093306 1105 42-43 0.9799121331397558 1105 44-45 0.9530656574351823 1105 46-47 0.9447657364732365 1105 48-49 0.637413368619768 1105 50-51 0.353891873623553 1105 52-53 0.42049629136967326 1105 54-55 0.5192027583913088 1105 56-57 0.6147869785842737 1105 58-59 0.5567454432348562 1105 60-61 0.8843699130762559 1105 62-63 0.9492110030918717 1105 64-65 1.352815824079041 1105 66-67 1.2468014384662318 1105 68-69 1.4070673254144204 1105 70-71 1.3944099724051178 1105 72-73 1.553717106123738 1105 74-75 1.7128825344131329 1105 76-77 1.6897166472411946 1105 78-79 1.924842415418535 1105 80-81 2.158087808320559 1105 82-83 2.536362274216355 1105 84-85 2.5730101150282287 1105 86-87 2.6863798726478976 1105 88-89 2.85030330097446 1105 90-91 3.1354977221416327 1105 92-93 3.3533110286784265 1105 94-95 3.446024243922672 1105 96-97 3.5627422202643846 1105 98-99 3.4873193768436366 1105 100-101 2.2798061537532046 1106 1 0.11235034069510164 1106 2 0.19076292959939778 1106 3 -0.003067936745626554 1106 4 0.08739347210067194 1106 5 0.10693574344097811 1106 6 -0.3949283146322884 1106 7 -0.056762844399635526 1106 8 -0.24110569703353946 1106 9 0.12464354448626125 1106 10-11 0.020096716289010885 1106 12-13 0.20625135522345062 1106 14-15 0.2925592365095184 1106 16-17 -0.20388619782971773 1106 18-19 0.17952088721535375 1106 20-21 0.36880050025541067 1106 22-23 0.32235285958532955 1106 24-25 0.2698013643999886 1106 26-27 0.1996072172296195 1106 28-29 0.21301334919591852 1106 30-31 -0.07662003242970172 1106 32-33 0.34846986397963065 1106 34-35 0.09929020221552065 1106 36-37 0.03168541447749362 1106 38-39 0.09816236755409591 1106 40-41 0.3101216411564849 1106 42-43 -0.1458252950854586 1106 44-45 0.3982572271135325 1106 46-47 0.7950974444577597 1106 48-49 0.6129797181825367 1106 50-51 0.35756648022697135 1106 52-53 0.5488198141937488 1106 54-55 0.4630644972177791 1106 56-57 1.1182085518332165 1106 58-59 1.0703413002354019 1106 60-61 1.087958207190983 1106 62-63 1.2203170306275233 1106 64-65 1.3834084937964768 1106 66-67 1.3472800463704289 1106 68-69 1.5111497842988406 1106 70-71 1.491749092392599 1106 72-73 1.716623259801203 1106 74-75 2.1802538833526874 1106 76-77 2.1025354106953067 1106 78-79 2.5212078255715475 1106 80-81 2.2210542214228113 1106 82-83 2.380020930346838 1106 84-85 2.545990838572422 1106 86-87 2.8491670069326958 1106 88-89 2.833667295077845 1106 90-91 2.9364846459401726 1106 92-93 2.635251101008482 1106 94-95 2.597713991292295 1106 96-97 2.705826959260479 1106 98-99 2.6783403476857597 1106 100-101 1.8200042507549448 1107 1 0.07492653575445019 1107 2 0.07764757651773024 1107 3 0.08113428761540575 1107 4 0.027026488177384067 1107 5 0.09906670982604027 1107 6 0.08779100237480719 1107 7 -0.029230138450181187 1107 8 0.06585879874231182 1107 9 0.22137294954098508 1107 10-11 0.18112004712706664 1107 12-13 0.19190351363788238 1107 14-15 0.23434843909034697 1107 16-17 0.2154241254868623 1107 18-19 0.2544286510908691 1107 20-21 0.3675653382406523 1107 22-23 0.3406147210618258 1107 24-25 0.5097168814569883 1107 26-27 0.369307427545813 1107 28-29 0.43547039478819016 1107 30-31 0.41585938153671265 1107 32-33 0.44736481282646423 1107 34-35 0.5526557067961306 1107 36-37 0.40181115860467287 1107 38-39 0.5475256263855925 1107 40-41 0.49940264497069364 1107 42-43 0.4862020281954713 1107 44-45 0.11682832467885618 1107 46-47 0.22765278613244533 1107 48-49 0.3014960978923753 1107 50-51 0.5502923027771445 1107 52-53 0.3279339018136156 1107 54-55 0.30439831178306775 1107 56-57 0.3161307883141653 1107 58-59 0.18438302482813285 1107 60-61 0.3061674896206661 1107 62-63 0.4016412205537385 1107 64-65 0.4743163289656245 1107 66-67 0.5655557209863353 1107 68-69 0.6716513349351345 1107 70-71 0.8575406298395727 1107 72-73 1.0308971342531343 1107 74-75 1.0947296078741324 1107 76-77 1.2482190274207916 1107 78-79 1.4985734370925954 1107 80-81 1.630763076090762 1107 82-83 1.8701779545510213 1107 84-85 2.0021096137479795 1107 86-87 1.990291842066192 1107 88-89 2.2742543089444567 1107 90-91 2.1681710965871623 1107 92-93 2.449344513477733 1107 94-95 2.390013388861181 1107 96-97 2.6301995753799705 1107 98-99 2.222848065220427 1107 100-101 1.4269580524873806 1108 1 0.31866502562553833 1108 2 0.29931219693925826 1108 3 0.019056325675563812 1108 4 0.1263768053453802 1108 5 0.18137692058928678 1108 6 0.1342868075722663 1108 7 0.23184516337097705 1108 8 0.3101344279626943 1108 9 0.27489249118207226 1108 10-11 0.03449379380191431 1108 12-13 0.064473597533933 1108 14-15 0.05387217553396795 1108 16-17 0.20394724805694864 1108 18-19 0.14621041904667464 1108 20-21 0.3406776770988458 1108 22-23 0.40625826515839947 1108 24-25 0.38537679551468784 1108 26-27 0.30579197839499983 1108 28-29 0.23475165118270525 1108 30-31 0.206175526410874 1108 32-33 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-1.2416126944225496 2315 90-91 -1.1628390044229384 2315 92-93 -1.09658982995661 2315 94-95 -1.2404748266270325 2315 96-97 -1.147140491960096 2315 98-99 -1.176478534106172 2315 100-101 -0.18758740205807278 2316 1 -0.8115075727726797 2316 2 -0.7698933364882983 2316 3 -0.5253644203949293 2316 4 -0.52544982534544 2316 5 -0.3488316536537184 2316 6 -0.24484034990771164 2316 7 -0.5849881057197805 2316 8 -0.5427329325238333 2316 9 -0.4732954397785676 2316 10-11 -0.5976891690658732 2316 12-13 -0.6563996732699522 2316 14-15 -0.8665839467839049 2316 16-17 -0.7970844877775676 2316 18-19 -0.7995054574362612 2316 20-21 -0.697473421449267 2316 22-23 -0.9405394563714253 2316 24-25 -1.0088619299125199 2316 26-27 -1.1759811168125012 2316 28-29 -1.155707469122234 2316 30-31 -0.7106393264736255 2316 32-33 -1.002419855562863 2316 34-35 -0.8803955421272107 2316 36-37 -0.9121832427734446 2316 38-39 -1.0944015915300014 2316 40-41 -1.0676946849173348 2316 42-43 -1.0345667056546546 2316 44-45 -1.249989074115284 2316 46-47 -1.1073472138675555 2316 48-49 -0.790536632512449 2316 50-51 -0.7346926240101013 2316 52-53 -0.8606083031820759 2316 54-55 -0.9001559517828248 2316 56-57 -1.1040780833481953 2316 58-59 -0.7172039691425773 2316 60-61 -0.9983157145136978 2316 62-63 -0.8929130430121539 2316 64-65 -1.0433300965296404 2316 66-67 -0.685208706231581 2316 68-69 -0.6244985358659001 2316 70-71 -0.6515980437177049 2316 72-73 -0.5453173360311077 2316 74-75 -0.8258194877331029 2316 76-77 -0.5984928588841179 2316 78-79 -0.7146974500736398 2316 80-81 -0.7482485518162143 2316 82-83 -0.40247923752477277 2316 84-85 -0.08912208306511005 2316 86-87 -0.07776586680547126 2316 88-89 -0.46377971345003033 2316 90-91 -0.19064027292188257 2316 92-93 -0.28088158471348024 2316 94-95 -0.4005593932232294 2316 96-97 -0.6380390120446631 2316 98-99 -0.5290683861146288 2316 100-101 -0.163855901000991 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6350.0 3 1317.0 4 1110.0 5 1167.0 6 1269.0 7 1598.0 8 2112.0 9 2713.0 10 3540.0 11 4865.0 12 6969.0 13 9146.0 14 10896.0 15 13429.0 16 16592.0 17 20062.0 18 17889.0 19 14723.0 20 15471.0 21 16733.0 22 18507.0 23 20713.0 24 23502.0 25 26198.0 26 29473.0 27 33188.0 28 37734.0 29 43038.0 30 48029.0 31 54877.0 32 62717.0 33 71685.0 34 82311.0 35 93018.0 36 98871.0 37 78191.0 38 20605.0 39 266.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.43890779017782 21.24319503945437 15.541725505617753 21.776171664750056 2 15.282715845366933 22.12893081449831 40.910156910980206 21.678196429154546 3 18.04644985843768 26.481110014853897 31.893431923911614 23.579008202796807 4 11.933290908605061 19.250170969015763 39.01557475774853 29.800963364630643 5 12.170739384964433 38.76133666198781 35.47323448998137 13.594689463066386 6 28.600598413007912 38.712254005833685 18.751626929179217 13.93552065197919 7 25.41845538158262 35.053072631226854 22.455076783618438 17.073395203572087 8 24.271468028908718 35.63396408280727 21.316073077345568 18.778494810938444 9 25.573388934684576 17.073170731707318 22.23305265470393 35.12038767890417 10-11 22.88309738659985 28.24948157032608 29.27392136692253 19.593499676151545 12-13 24.334385544791555 26.67717228890626 28.832045281375855 20.156396884926327 14-15 21.45850062265022 28.20306164806137 28.203407977146238 22.13502975214217 16-17 19.38136597780034 30.699028146353918 29.425783112979225 20.49382276286652 18-19 19.034529027508338 28.960319498270554 32.71893641748909 19.28621505673202 20-21 20.630083412748128 28.13544947784154 32.54264896087844 18.69181814853189 22-23 19.9965562335883 28.581578917420362 31.980368552277216 19.44149629671412 24-25 19.521209530086832 28.654341367441177 32.53833470896895 19.28611439350304 26-27 19.380568762824822 28.84611102616895 32.390772213323906 19.382547997682316 28-29 19.17531139322678 28.928225432170358 32.55391429722158 19.34254887738128 30-31 19.900733833375146 28.731672102011302 32.35170563934942 19.015888425264134 32-33 19.196886897708467 28.929042345019013 32.46918192005977 19.404888837212756 34-35 19.658355206681495 28.974694535909812 32.27195024503922 19.09500001236947 36-37 19.609027720052406 28.78627297304786 31.703350314487817 19.90134899241192 38-39 19.204079926427557 29.215364134620952 32.02756097355854 19.55299496539295 40-41 19.670437849683648 28.657363205983767 32.16520650813517 19.50699243619742 42-43 19.318809177525157 28.78370088945614 31.88007677619147 20.017413156827242 44-45 19.9071987732034 28.817501638614413 31.497953277847166 19.77734631033502 46-47 19.811296485753243 28.770607290493782 30.929042254468747 20.489053969284228 48-49 19.70770998759781 28.77392443023879 31.07453830654437 20.443827275619032 50-51 19.897414030206914 29.000842928404513 30.39642368826687 20.705319353121705 52-53 19.827173843682804 29.242979091982512 30.497005049048536 20.432842015286145 54-55 20.134458919342457 28.8983292309397 30.310637599031253 20.656574250686592 56-57 20.113021011876405 29.149458515184477 29.959125848164618 20.778394624774496 58-59 19.952702375169785 28.886868552965723 30.194601877857174 20.965827194007318 60-61 20.130540166204984 28.808913731697665 30.092303126236647 20.968242975860704 62-63 19.940955408372794 28.705386978035868 30.293150263439987 21.060507350151347 64-65 20.001780767708745 28.601503759398494 30.085427384250096 21.31128808864266 66-67 19.975208626922978 28.81636032766954 29.796984771508324 21.411446273899166 68-69 20.197011682790812 28.40553500533734 29.911070703193403 21.486382608678447 70-71 20.015196981974363 28.19173723468355 30.105211397340724 21.687854386001362 72-73 20.096116744991342 27.901904526341823 30.184071234232007 21.817907494434827 74-75 20.137582093155327 28.07165428962089 29.678839901775135 22.11192371544865 76-77 19.970766091634204 27.977097631855564 30.00734557595993 22.044790700550298 78-79 20.048714347829744 27.941398341199292 29.679889156061517 22.32999815490945 80-81 20.215470886844887 27.811217862149682 29.495935248915917 22.47737600208951 82-83 20.359992955670194 27.77622776138718 29.524992530240162 22.338786752702468 84-85 20.431541509405964 27.319189350956425 29.533139756330414 22.716129383307198 86-87 20.300658903206372 27.4766742527179 29.45175157652522 22.770915267550514 88-89 20.89932942349219 27.181179334073747 29.422799601265464 22.4966916411686 90-91 20.99260189402991 27.06159184204576 29.202208794508987 22.74359746941534 92-93 21.02786124090906 26.78699868118909 29.3667926775819 22.818347400319958 94-95 21.046978630605423 26.681119397299362 29.22972420746628 23.04217776462894 96-97 21.34926736501736 26.43105988421508 28.8424377168741 23.37723503389346 98-99 21.440878481159185 26.159479472804453 28.686774946586404 23.712867099449962 100-101 22.457418104882805 25.32379673211937 29.046022916369513 23.172762246628313 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1847.0 1 1211.0 2 532.5 3 441.5 4 326.5 5 322.0 6 491.0 7 790.0 8 1179.5 9 1280.5 10 1033.0 11 848.0 12 804.0 13 852.5 14 1006.0 15 1150.5 16 1288.5 17 1442.0 18 1660.5 19 1943.0 20 2275.5 21 2766.5 22 3293.0 23 3884.0 24 4743.0 25 5981.0 26 7956.5 27 10421.5 28 12212.0 29 14245.0 30 17185.0 31 20137.0 32 23306.0 33 27138.5 34 31273.0 35 35157.0 36 39067.5 37 42149.5 38 42209.5 39 40336.5 40 38747.5 41 37734.0 42 37370.0 43 37878.5 44 38131.0 45 37613.0 46 37024.5 47 36472.5 48 35847.0 49 34724.0 50 33017.5 51 30767.0 52 28221.5 53 25543.5 54 22610.0 55 19530.0 56 16501.5 57 13582.5 58 10983.0 59 8585.0 60 6472.0 61 4822.5 62 3521.5 63 2495.0 64 1748.5 65 1249.0 66 946.5 67 714.5 68 556.0 69 434.0 70 356.0 71 303.5 72 234.5 73 201.5 74 158.0 75 131.0 76 112.0 77 74.5 78 51.0 79 40.5 80 30.5 81 22.5 82 18.5 83 12.0 84 6.0 85 4.0 86 4.5 87 3.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38461766748378134 2 0.029776213454891513 3 0.036107368475200664 4 0.04520840381689508 5 0.06489433895816887 6 0.05292449899789687 7 0.036799838555546985 8 0.03788800582466262 9 0.03432673112573872 10-11 0.03665145210975849 12-13 0.030072986346468503 14-15 0.02715471957929475 16-17 0.022554739759851374 18-19 0.028638584037179705 20-21 0.035315974097662024 22-23 0.034870814760296534 24-25 0.04273529638708682 26-27 0.037838543676066455 28-29 0.03313963955943075 30-31 0.03546436054345052 32-33 0.03032029708944933 34-35 0.031804161547334286 36-37 0.033832109639777064 38-39 0.016767668374100035 40-41 0.013750477309733953 42-43 0.014245098795695606 44-45 0.011030059136944862 46-47 0.018597767872158152 48-49 0.016371971185330715 50-51 0.011079521285541027 52-53 0.00885372459871359 54-55 0.00811179236977111 56-57 0.008754800301521258 58-59 0.004055896184885555 60-61 0.007320397992232464 62-63 0.011128983434137192 64-65 0.007320397992232464 66-67 0.003808585441904728 68-69 0.007023625100655473 70-71 0.014541871687272598 72-73 0.012266612851848994 74-75 0.013058007229387639 76-77 0.006083844277328332 78-79 0.008260178815559604 80-81 0.010980596988348697 82-83 0.013058007229387639 84-85 0.007815019478194117 86-87 0.0027204181727890912 88-89 0.01746013845444635 90-91 0.027451492470871742 92-93 0.011376294177118019 94-95 0.022060118273889723 96-97 0.020477329518812433 98-99 0.012810696486406812 100-101 0.12761234337810648 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1010874.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.944249992303526 #Duplication Level Percentage of deduplicated Percentage of total 1 77.28230764680177 47.09912277208797 2 13.347889775651502 16.26954262714035 3 4.557569872123317 8.332730329322226 4 1.902958411598569 4.6389749264568 5 1.0025469693717786 3.054973656520996 6 0.5409821768359371 1.978185181588194 7 0.32583625102557706 1.3900492157340414 8 0.1999086136010335 0.9746624418332955 9 0.1329582953523052 0.7292739231451273 >10 0.6151717678450062 6.7763070468425575 >50 0.055228603156170036 2.3336961192087524 >100 0.03452454604888398 3.733438124803601 >500 0.0011399610859035008 0.43401446140243094 >1k 6.514063348020006E-4 0.9548715145558628 >5k 3.257031674010003E-4 1.3001576593578428 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7180 0.7102764538409337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5920 0.5856318393785971 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4025 0.39817029619913064 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3017 0.29845460462926143 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1543 0.1526401905677661 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.1024855718912545 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.89242971923306E-5 0.0 10-11 0.0 0.0 0.0 9.89242971923306E-5 0.0 12-13 0.0 0.0 0.0 1.483864457884959E-4 0.0 14-15 0.0 0.0 0.0 1.978485943846612E-4 0.0 16-17 0.0 0.0 0.0 1.978485943846612E-4 0.0 18-19 0.0 0.0 0.0 3.956971887693224E-4 0.0 20-21 0.0 0.0 0.0 7.419322289424794E-4 0.0 22-23 0.0 0.0 0.0 0.002621493875596761 0.0 24-25 0.0 0.0 0.0 0.005984919980136002 0.0 26-27 0.0 0.0 0.0 0.011722529217291177 0.0 28-29 0.0 0.0 0.0 0.028143962551218055 0.0 30-31 0.0 0.0 0.0 0.07102764538409337 0.0 32-33 0.0 0.0 0.0 0.14719935422218794 0.0 34-35 0.0 0.0 0.0 0.2729321359536401 0.0 36-37 0.0 0.0 0.0 0.46192700573958767 0.0 38-39 0.0 0.0 0.0 0.6896507378763328 0.0 40-41 0.0 0.0 0.0 0.9279099076640609 0.0 42-43 0.0 0.0 0.0 1.1615196354837498 0.0 44-45 0.0 0.0 0.0 1.4496366510564127 0.0 46-47 0.0 0.0 0.0 1.779202947152662 0.0 48-49 0.0 0.0 0.0 2.1187605972653367 0.0 50-51 0.0 0.0 0.0 2.456042988542588 0.0 52-53 0.0 0.0 0.0 2.7782394244980084 0.0 54-55 0.0 0.0 0.0 3.14970016045521 0.0 56-57 0.0 0.0 0.0 3.5893197371779273 0.0 58-59 0.0 0.0 0.0 4.04654783880088 0.0 60-61 0.0 0.0 0.0 4.511640422050622 0.0 62-63 0.0 0.0 0.0 4.956008365038571 0.0 64-65 0.0 0.0 0.0 5.440589034835202 0.0 66-67 0.0 0.0 0.0 5.992784461762792 0.0 68-69 0.0 0.0 0.0 6.60225705676474 0.0 70-71 0.0 0.0 0.0 7.244523056285947 0.0 72-73 0.0 0.0 0.0 7.892229892152732 0.0 74-75 0.0 0.0 0.0 8.531033541272206 0.0 76-77 0.0 0.0 0.0 9.227213282763232 0.0 78-79 0.0 0.0 0.0 9.996597004176584 0.0 80-81 0.0 0.0 0.0 10.802236480510924 0.0 82-83 0.0 0.0 0.0 11.58368896618174 0.0 84-85 0.0 0.0 0.0 12.341102847634819 0.0 86-87 0.0 0.0 0.0 13.139075690936753 0.0 88-89 0.0 0.0 0.0 13.959009728215387 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3145 0.0 51.842236 1 GTATCAA 5740 0.0 43.105606 1 TATCAAC 7210 0.0 34.201588 2 ATCAACG 7285 0.0 33.653816 3 TCAACGC 7395 0.0 33.15322 4 CAACGCA 7600 0.0 32.132328 5 GTGGTAT 1385 0.0 32.011898 1 AACGCAG 7645 0.0 31.93845 6 CCGCGAC 30 0.0039515495 31.66684 90-91 ACGCAGA 8505 0.0 28.59439 7 TAGGACG 335 0.0 28.366083 4 CGCAGAG 8565 0.0 28.283167 8 GCAGAGT 8925 0.0 26.663353 9 TGGTATC 1680 0.0 26.301947 2 GTACATG 4460 0.0 26.081541 1 AGGACGT 470 0.0 25.271723 5 TACATGG 4680 0.0 24.771833 2 CGTATAG 135 1.3050871E-5 24.719664 1 ACATGGG 4660 0.0 23.858553 3 GACGCTC 165 0.0 23.051529 94-95 >>END_MODULE