FastQCFastQC Report
Thu 26 May 2016
SRR938668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938668_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010874
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71050.7028571315515089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62030.6136274154840267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42330.4187465500151354No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32260.3191297827424585No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT25030.24760751587240346No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.15541007088915137No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT15340.15174987189303513RNA PCR Primer, Index 33 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10230.1011995560277542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32300.056.186711
CCAGTCG451.5159789E-552.776958
GTATCAA56950.039.0413551
ATCAACG72500.030.4650443
TCAACGC72500.030.4650444
TATCAAC72950.030.3452262
GTGGTAT13000.029.9671
CAACGCA74350.029.7708875
AACGCAG76200.029.0481016
CATTCCG2450.027.1424319
CTCCGAC2950.025.7623194-95
ACGCAGA86000.025.6275047
CGCAGAG86800.025.3365868
TCGTATC751.0609219E-625.33293794-95
TGGTATC15450.024.290052
AGCCGAA500.001654229223.74962814-15
GCAGAGT90800.023.4880719
GAGTACT62600.023.37024112-13
GTACTTT63150.022.64018614-15
GTATAGC7050.022.2380941
Produced by FastQC (version 0.11.5)