##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938668_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1010874 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.780118986144664 31.0 31.0 34.0 30.0 34.0 2 32.040387822814715 31.0 31.0 34.0 30.0 34.0 3 32.076334933928464 33.0 31.0 34.0 30.0 34.0 4 35.75301076098505 37.0 35.0 37.0 35.0 37.0 5 35.65086548867614 37.0 35.0 37.0 33.0 37.0 6 35.68340169002269 37.0 35.0 37.0 33.0 37.0 7 35.61106230845783 37.0 35.0 37.0 33.0 37.0 8 35.595516355154054 37.0 35.0 37.0 33.0 37.0 9 37.1162251675283 39.0 37.0 39.0 33.0 39.0 10-11 37.069578404430224 39.0 37.0 39.0 33.0 39.0 12-13 36.97216517587751 39.0 37.0 39.0 33.0 39.0 14-15 37.9624339927627 40.0 37.0 41.0 33.0 41.0 16-17 38.032705361894756 40.0 37.0 41.0 33.0 41.0 18-19 38.22308072024802 40.0 37.5 41.0 33.0 41.0 20-21 38.22788102176928 40.0 37.5 41.0 33.0 41.0 22-23 38.0850857772581 40.0 37.0 41.0 33.0 41.0 24-25 37.98224457251843 40.0 37.0 41.0 33.0 41.0 26-27 37.77474838605009 40.0 37.0 41.0 32.0 41.0 28-29 37.626986152576876 39.5 37.0 41.0 32.0 41.0 30-31 37.33811038764475 39.0 36.0 41.0 32.0 41.0 32-33 37.133241135888355 39.0 36.0 41.0 31.0 41.0 34-35 36.95315983990092 39.0 35.0 41.0 31.0 41.0 36-37 36.80714708262355 39.0 35.0 41.0 31.0 41.0 38-39 36.56912434190611 39.0 35.0 41.0 30.0 41.0 40-41 36.350889922977544 39.0 35.0 40.0 30.0 41.0 42-43 36.044567374371084 39.0 35.0 40.0 30.0 41.0 44-45 35.650756671949225 38.0 35.0 40.0 28.5 41.0 46-47 35.093734728561614 38.0 34.5 40.0 26.0 41.0 48-49 34.605943470699614 38.0 34.0 40.0 24.0 41.0 50-51 34.198901148906785 38.0 33.5 40.0 23.0 41.0 52-53 33.6737615172613 38.0 33.0 40.0 20.0 41.0 54-55 33.305535605822286 38.0 33.0 40.0 18.0 41.0 56-57 32.95428164143108 37.0 32.0 40.0 14.5 41.0 58-59 32.486471113115975 37.0 31.5 40.0 10.0 41.0 60-61 32.0936194817554 36.5 31.0 39.0 8.0 40.0 62-63 31.674907060622786 36.0 31.0 39.0 4.5 40.0 64-65 31.11050833239355 36.0 29.5 39.0 2.0 40.0 66-67 30.51778312628478 35.0 28.5 38.5 2.0 40.0 68-69 29.860516246337326 34.5 28.0 38.0 2.0 40.0 70-71 29.187960616258803 34.0 27.0 37.5 2.0 39.0 72-73 28.674331815834613 34.0 26.0 37.0 2.0 39.0 74-75 28.02941612901311 33.0 26.0 36.0 2.0 39.0 76-77 26.806471429673728 31.5 25.0 34.5 2.0 37.0 78-79 27.336773920389682 33.0 26.0 35.0 2.0 37.0 80-81 26.97825347174821 33.0 25.0 35.0 2.0 37.0 82-83 26.3732013089663 32.0 23.5 35.0 2.0 36.5 84-85 25.680517057516568 32.0 21.0 34.5 2.0 36.0 86-87 24.870989856302565 31.0 19.0 34.0 2.0 35.5 88-89 24.00576530804037 30.5 11.0 34.0 2.0 35.0 90-91 23.23689797145836 30.0 4.5 34.0 2.0 35.0 92-93 22.26774256732293 29.0 2.0 34.0 2.0 35.0 94-95 21.194531662699802 29.0 2.0 34.0 2.0 35.0 96-97 19.886127746880423 27.5 2.0 33.0 2.0 34.5 98-99 18.16501265241761 24.5 2.0 33.0 2.0 34.0 100-101 13.772334633198598 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24837626320824313 1101 2 0.31283963387247127 1101 3 0.24827561984945135 1101 4 0.12616694724507482 1101 5 0.19781273080030815 1101 6 0.23514456426961772 1101 7 0.25991341191462425 1101 8 0.38803730308794115 1101 9 0.5187967163945331 1101 10-11 0.4374197237242612 1101 12-13 0.3718757931726415 1101 14-15 0.6607452987106015 1101 16-17 0.48810548110679974 1101 18-19 0.4409374542349056 1101 20-21 0.5213634369699918 1101 22-23 0.4567873577474231 1101 24-25 0.3901680920666166 1101 26-27 0.6680761713866943 1101 28-29 0.6550820845765841 1101 30-31 0.6704130819741323 1101 32-33 0.6658497779554438 1101 34-35 0.5965245514537969 1101 36-37 0.4109437095248438 1101 38-39 0.6750089660929106 1101 40-41 0.6723160334568163 1101 42-43 0.8281742470379427 1101 44-45 0.7848063003888299 1101 46-47 0.9066916573154202 1101 48-49 0.8568008369732709 1101 50-51 0.8962850707021346 1101 52-53 1.1039286490391333 1101 54-55 0.853840362039584 1101 56-57 1.1138280923592845 1101 58-59 1.31392157018157 1101 60-61 1.2822727278645019 1101 62-63 1.2615180471414256 1101 64-65 1.2460659429561503 1101 66-67 1.3436692006758904 1101 68-69 1.4197504985890816 1101 70-71 1.5914448231962695 1101 72-73 1.8302022279918155 1101 74-75 1.567502383492215 1101 76-77 1.8410219240105796 1101 78-79 1.7614155804398557 1101 80-81 1.6401140615551313 1101 82-83 1.9994043086437365 1101 84-85 2.293655606066359 1101 86-87 2.326726514432213 1101 88-89 2.3843749573093795 1101 90-91 2.6921828567693105 1101 92-93 2.7188205077640824 1101 94-95 2.6108041893213247 1101 96-97 3.0029729855373795 1101 98-99 3.1240395905013223 1101 100-101 2.5934733334306443 1102 1 0.1949603333799672 1102 2 0.29522728440223034 1102 3 0.17376581592787943 1102 4 0.21084600338598136 1102 5 0.09651907202266585 1102 6 0.15316107180272098 1102 7 0.10825942298632185 1102 8 0.1084964078219457 1102 9 0.3098852370113008 1102 10-11 0.2782749857210476 1102 12-13 0.20958838796152435 1102 14-15 0.4085641982476105 1102 16-17 0.26506435346462354 1102 18-19 0.36678415655219254 1102 20-21 0.2120813322889319 1102 22-23 0.2905213193788754 1102 24-25 0.25573288383991155 1102 26-27 0.17559457028540493 1102 28-29 0.3290988980226288 1102 30-31 0.5498016090621363 1102 32-33 0.42088998785878573 1102 34-35 0.37805035109258966 1102 36-37 0.47463419622057756 1102 38-39 0.48312977817411706 1102 40-41 0.4666007560467236 1102 42-43 0.49953020616326427 1102 44-45 0.4115307375384276 1102 46-47 0.5136588246203857 1102 48-49 0.7192317859505266 1102 50-51 0.8494833317047039 1102 52-53 0.7253413785473839 1102 54-55 0.7862124227750726 1102 56-57 0.9040629982028179 1102 58-59 0.9975022811938459 1102 60-61 1.0941627403951628 1102 62-63 0.9550879923712436 1102 64-65 1.1467883681398447 1102 66-67 1.1721538138430887 1102 68-69 1.5744112587827495 1102 70-71 1.6388166191993996 1102 72-73 1.5598134244055721 1102 74-75 1.4722369745983848 1102 76-77 1.4046847608824997 1102 78-79 1.4895572181330365 1102 80-81 1.767123821354641 1102 82-83 2.157361280811539 1102 84-85 2.1985313877621735 1102 86-87 2.3105260708584403 1102 88-89 2.0424537063607566 1102 90-91 2.071299591972238 1102 92-93 2.1688755132646307 1102 94-95 1.682606075392286 1102 96-97 2.2516249095533105 1102 98-99 2.274878792068538 1102 100-101 2.4251206158059375 1103 1 0.2822480714416997 1103 2 0.2717166361237844 1103 3 0.26685147124263864 1103 4 0.1275361244104687 1103 5 0.2843978599014534 1103 6 0.22421839425323498 1103 7 0.2974704183892669 1103 8 0.0469146459305847 1103 9 0.32613441610877203 1103 10-11 0.39955277913506393 1103 12-13 0.49125646188966954 1103 14-15 0.4465133491998543 1103 16-17 0.3449123692259519 1103 18-19 0.41255768127308556 1103 20-21 0.3387911549739826 1103 22-23 0.5400006326352766 1103 24-25 0.44225131896515535 1103 26-27 0.5580413288931538 1103 28-29 0.5534445214394665 1103 30-31 0.526835101885105 1103 32-33 0.5254495150189342 1103 34-35 0.5572134996055809 1103 36-37 0.6924407446772776 1103 38-39 0.7165100202230903 1103 40-41 0.6986863175440519 1103 42-43 0.6015754261651409 1103 44-45 0.791444425501652 1103 46-47 0.9330415059225672 1103 48-49 0.9385018863351746 1103 50-51 0.8135043468093102 1103 52-53 0.9170291541360882 1103 54-55 0.9090944942639467 1103 56-57 1.0418338960356266 1103 58-59 1.0509969805089767 1103 60-61 0.8272052396915299 1103 62-63 0.9411748673126077 1103 64-65 1.11085629196009 1103 66-67 0.8643482035606986 1103 68-69 0.9181020365292518 1103 70-71 1.1100428130262152 1103 72-73 1.083127056078336 1103 74-75 0.9842925145177013 1103 76-77 1.0964194451868465 1103 78-79 1.2971282848372319 1103 80-81 1.573719186773534 1103 82-83 1.5757870243146819 1103 84-85 1.7994320351024484 1103 86-87 1.7791816670682188 1103 88-89 1.935586270391152 1103 90-91 2.35493783195723 1103 92-93 2.341630931221289 1103 94-95 1.9952245198992777 1103 96-97 2.145958336704073 1103 98-99 2.1263845618405632 1103 100-101 2.143977919492965 1104 1 0.19109361186618656 1104 2 0.36728463881252793 1104 3 0.43142580299963385 1104 4 0.28424319443023194 1104 5 0.18800405762529948 1104 6 0.1315473913655083 1104 7 0.1923165423375579 1104 8 0.14350845459870243 1104 9 0.1283961378751428 1104 10-11 0.21807636081653925 1104 12-13 0.33971708279610624 1104 14-15 0.5786881919010156 1104 16-17 0.40626432995871653 1104 18-19 0.35269236598981735 1104 20-21 0.3199323048457856 1104 22-23 0.45612630263091347 1104 24-25 0.12085599602185226 1104 26-27 0.4134743375769361 1104 28-29 0.3098475611242364 1104 30-31 0.4122097891173766 1104 32-33 0.5090665172117923 1104 34-35 0.38596008841408747 1104 36-37 0.34795499307078614 1104 38-39 0.5444684395127766 1104 40-41 0.4095930578625584 1104 42-43 0.3939945653335073 1104 44-45 0.4983556773045379 1104 46-47 0.5146343197135295 1104 48-49 0.41460112249632886 1104 50-51 0.4941915646482329 1104 52-53 0.30103627188933046 1104 54-55 0.4523051535736826 1104 56-57 0.4816912523016583 1104 58-59 0.5431389974187937 1104 60-61 0.6536620646003755 1104 62-63 0.8544592093895211 1104 64-65 0.9328846248243394 1104 66-67 1.0475901422205958 1104 68-69 0.9923286355236556 1104 70-71 0.9318706240181136 1104 72-73 1.1627986744496432 1104 74-75 0.9016017274925474 1104 76-77 0.9431227934381816 1104 78-79 1.2069986002803041 1104 80-81 1.3591788251743502 1104 82-83 1.3785812373256157 1104 84-85 1.3461190941734102 1104 86-87 1.4399378355643258 1104 88-89 1.1632858154281571 1104 90-91 1.4616680470465795 1104 92-93 1.6984459428350611 1104 94-95 1.3784749124375928 1104 96-97 1.7565670262601323 1104 98-99 2.105142645861804 1104 100-101 1.8501896644044002 1105 1 0.2218097311899001 1105 2 0.1337080887802813 1105 3 0.17297539265048556 1105 4 0.24243689945937774 1105 5 0.22943098512817528 1105 6 0.14326984612593918 1105 7 0.10561843032299123 1105 8 0.300263092365995 1105 9 0.4524644746017046 1105 10-11 0.30951024338966704 1105 12-13 0.2852841739328156 1105 14-15 0.4972316737356337 1105 16-17 0.5504555817449059 1105 18-19 0.3073769219401967 1105 20-21 0.25864549039442153 1105 22-23 0.552093626446343 1105 24-25 0.3748045790843548 1105 26-27 0.24322054074085742 1105 28-29 0.35335468690984584 1105 30-31 0.42237683725910813 1105 32-33 0.40881234100824315 1105 34-35 0.3997880326217782 1105 36-37 0.4708257931567701 1105 38-39 0.5429952291788069 1105 40-41 0.5371364005957702 1105 42-43 0.3579736242492757 1105 44-45 0.4822262371244719 1105 46-47 0.5279817317951156 1105 48-49 0.6695866433046405 1105 50-51 0.5708174703880644 1105 52-53 0.5219672396557442 1105 54-55 0.7611735004167102 1105 56-57 0.616360380641666 1105 58-59 0.6363116475534696 1105 60-61 0.6935462310668115 1105 62-63 0.6505365497706599 1105 64-65 0.7944407901779726 1105 66-67 0.8259067623093692 1105 68-69 0.7515504263910167 1105 70-71 0.860732900981656 1105 72-73 1.10197974654211 1105 74-75 1.2703848431111098 1105 76-77 1.1460148381023778 1105 78-79 1.1702189242601193 1105 80-81 1.376275652397755 1105 82-83 1.2922435244056238 1105 84-85 1.688567784723368 1105 86-87 1.856110270724102 1105 88-89 1.4768709644563458 1105 90-91 1.4893871633732907 1105 92-93 1.9602094957378569 1105 94-95 1.9346339432801685 1105 96-97 2.393272093344944 1105 98-99 2.045771637123707 1105 100-101 2.2035641380320374 1106 1 0.06385319107427279 1106 2 0.12002179578452399 1106 3 0.06797404782506788 1106 4 0.09043507761922598 1106 5 0.21653471849474926 1106 6 -0.019208493750333844 1106 7 -0.06345751998125593 1106 8 0.05805300533189239 1106 9 -0.1420898727456148 1106 10-11 0.13761062828150727 1106 12-13 0.060332367415234955 1106 14-15 0.06503149248877804 1106 16-17 0.07114949291894845 1106 18-19 0.09525250075399327 1106 20-21 0.01184097365532466 1106 22-23 0.13359801282640404 1106 24-25 0.17314730432855896 1106 26-27 -0.020423814319293854 1106 28-29 0.2133386802072721 1106 30-31 0.23744038143170343 1106 32-33 0.10936564061004361 1106 34-35 0.20974217735498257 1106 36-37 0.18500821895942465 1106 38-39 0.4449462100579211 1106 40-41 0.21782236464816407 1106 42-43 0.37782690239009753 1106 44-45 0.18224301229871287 1106 46-47 -0.4132587689110352 1106 48-49 -0.08558156296065533 1106 50-51 0.043255109380687884 1106 52-53 0.029919515690771448 1106 54-55 0.08227095293312914 1106 56-57 0.18297937707389877 1106 58-59 0.40269907160831764 1106 60-61 0.41854395845242465 1106 62-63 0.2537601009907 1106 64-65 0.5024445163284064 1106 66-67 0.7052337498160774 1106 68-69 0.702271243445086 1106 70-71 0.47254506026521526 1106 72-73 0.9061717594974148 1106 74-75 0.8241281904424937 1106 76-77 0.7477820417932897 1106 78-79 0.9963165970092724 1106 80-81 1.1706921434249153 1106 82-83 1.3569104153660518 1106 84-85 1.430608714274193 1106 86-87 1.154217300084408 1106 88-89 1.4113414171892487 1106 90-91 1.4398929020760534 1106 92-93 1.57878889151295 1106 94-95 1.4598646487840163 1106 96-97 1.470680150864112 1106 98-99 1.6761794050602568 1106 100-101 1.574658169912368 1107 1 0.08429485506743006 1107 2 0.1783328498607375 1107 3 0.0556830272568547 1107 4 0.09457545476249152 1107 5 0.06468108430232178 1107 6 -0.08853530371718676 1107 7 -0.21409648907547307 1107 8 -0.05257509148484729 1107 9 -0.051958692479686874 1107 10-11 0.021701901942080326 1107 12-13 0.17302174110076152 1107 14-15 0.16849190670473035 1107 16-17 0.14127746497185 1107 18-19 0.1076041209015628 1107 20-21 0.055691453104934396 1107 22-23 0.2687373027419184 1107 24-25 0.2905714657373153 1107 26-27 0.1502727243753199 1107 28-29 0.2029872917639679 1107 30-31 0.16875833316591837 1107 32-33 0.0733855315308034 1107 34-35 0.22322513817913858 1107 36-37 0.06313934575513258 1107 38-39 0.07997321251755096 1107 40-41 0.30143686220635857 1107 42-43 0.13156625290519486 1107 44-45 0.1309009098497782 1107 46-47 0.19520467528388252 1107 48-49 0.20451352490873376 1107 50-51 0.05659860205526712 1107 52-53 0.13226106561413076 1107 54-55 0.23786126412979058 1107 56-57 0.08445515506555523 1107 58-59 0.2510227803025771 1107 60-61 -0.01382646538815635 1107 62-63 0.4532534706837801 1107 64-65 0.3772448941846065 1107 66-67 0.3938388009692524 1107 68-69 0.5431278363228564 1107 70-71 0.34828384043132843 1107 72-73 0.7223456089965801 1107 74-75 0.6838481847390057 1107 76-77 0.6207844835998735 1107 78-79 0.45437946016116015 1107 80-81 0.4618183153138382 1107 82-83 0.6700222037665817 1107 84-85 0.846667157221475 1107 86-87 1.128321673948161 1107 88-89 1.0197709969133406 1107 90-91 1.4790090393875701 1107 92-93 1.8059125503016702 1107 94-95 1.5215073429700219 1107 96-97 1.6404271476202545 1107 98-99 1.63413309985226 1107 100-101 1.5054186946334305 1108 1 -0.030100479493651022 1108 2 -0.06971298603039955 1108 3 -0.0427004390824095 1108 4 -0.006785298255721273 1108 5 0.1353890851208206 1108 6 -0.04481216068464988 1108 7 -0.017426753041100085 1108 8 0.09271854469276519 1108 9 0.2064718374978014 1108 10-11 -0.0442050962162952 1108 12-13 -0.012925670399134503 1108 14-15 0.14979434173644535 1108 16-17 0.09980007598065299 1108 18-19 0.08826710432698093 1108 20-21 0.0886129796038162 1108 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0.25346547664695507 2316 98-99 0.21970157530445178 2316 100-101 -0.02037372569387408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 15.0 5 43.0 6 142.0 7 278.0 8 598.0 9 1155.0 10 2056.0 11 3042.0 12 4433.0 13 5828.0 14 7558.0 15 10434.0 16 13708.0 17 17564.0 18 15806.0 19 11924.0 20 11276.0 21 11798.0 22 12525.0 23 14090.0 24 15824.0 25 17657.0 26 20308.0 27 23404.0 28 27660.0 29 32888.0 30 38876.0 31 45892.0 32 54556.0 33 66976.0 34 83213.0 35 105590.0 36 133617.0 37 143314.0 38 55644.0 39 1174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.03431631568636 22.081579421293135 16.01982192721537 21.86428233580514 2 15.171497431594943 22.627589181830416 40.560616733811926 21.640296652762718 3 17.80948895000297 26.784915814257165 32.275686049502404 23.12990918623746 4 11.988734501035738 20.10201073526473 38.49846766263649 29.41078710106304 5 11.94023354186699 39.20887791482171 35.474265680583414 13.376622862727874 6 28.24783306327 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19.232540504229615 28-29 19.115288144932368 29.13570122414174 32.39882616138649 19.350184469539403 30-31 19.946501740078386 28.872391613593777 32.2450671399205 18.936039506407326 32-33 19.188136579111895 29.12594275041746 32.370814504704846 19.315106165765794 34-35 19.610160327550805 29.00305656701866 32.25017053754324 19.136612567887298 36-37 19.444852510197936 28.92533287036419 31.785453370944627 19.844361248493247 38-39 19.070489672107467 29.149262236855904 32.124652983206666 19.655595107829964 40-41 19.586647671059403 28.690242985476278 32.308312556887884 19.41479678657644 42-43 19.406181059129626 28.67005332251714 31.998258856583206 19.92550676177003 44-45 19.705292877012624 28.88670924393689 31.64137157373663 19.76662630531385 46-47 19.799816594171574 28.720966926607 30.935092736674143 20.54412374254728 48-49 19.677477113863844 28.702340746720168 31.09492379861387 20.525258340802118 50-51 19.769427248104115 28.955141788195167 30.513545704014543 20.76188525968617 52-53 19.808403421197895 29.250875974651635 30.5206187912638 20.42010181288667 54-55 19.98785209630478 28.998619016811194 30.122349570767476 20.89117931611655 56-57 20.201973737577582 29.29662846210309 29.60774537677297 20.893652423546357 58-59 19.671145958843535 29.288615594030514 30.001464079598446 21.038774367527505 60-61 20.07000872512301 29.380813039013766 29.57604013952283 20.973138096340392 62-63 19.69805336767985 29.375075429776608 29.79456391202069 21.132307290522853 64-65 19.6292709371009 29.351164785289548 29.59995963884682 21.41960463876273 66-67 19.604222692993126 29.526255315339288 29.267221402522477 21.60230058914511 68-69 19.85234435243858 29.074738429593257 29.291384462126764 21.781532755841404 70-71 19.596230189153335 28.957495155177753 29.54129747258063 21.90497718308828 72-73 19.72419865017596 28.654207132947036 29.435957551521085 22.18563666535592 74-75 19.695283487358463 28.948464398134682 28.816499385680117 22.539752728826738 76-77 19.279010044773138 28.725043872925802 29.68460955569141 22.311336526609647 78-79 19.20570714055362 28.754572775637715 29.29924995597869 22.740470127829976 80-81 19.261123800912678 28.807476310080492 29.16464283841788 22.766757050588946 82-83 19.347617160971783 28.819073989712756 29.124011303207602 22.709297546107855 84-85 19.305961234539197 28.512770540821954 29.05440866225277 23.126859562386077 86-87 19.075017275227893 28.588981149845104 29.03677682087332 23.299224754053682 88-89 19.346229104715327 28.547672608059955 28.766691002043775 23.339407285180943 90-91 19.302376194527433 28.363522149456898 28.9175058860772 23.416595769938468 92-93 19.157131141621715 28.347293269454298 28.790723624920737 23.704851964003247 94-95 19.107574237738827 28.25441152903329 28.769609268810946 23.868404964416932 96-97 19.20001899346506 28.211973005537782 28.445533271208873 24.142474729788283 98-99 19.131711766253755 28.09667673716012 28.378512059861073 24.393099436725052 100-101 18.92220882346249 28.285303890795383 28.630104869751193 24.16238241599093 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2042.0 1 1246.5 2 463.5 3 455.0 4 365.0 5 352.0 6 531.5 7 869.0 8 1185.5 9 1282.0 10 1069.5 11 841.5 12 832.0 13 898.0 14 1055.5 15 1216.5 16 1338.5 17 1496.0 18 1762.0 19 2097.5 20 2412.5 21 2846.0 22 3378.5 23 4004.5 24 4917.0 25 6265.0 26 8325.5 27 10818.0 28 12586.0 29 14511.0 30 17374.0 31 20276.5 32 23583.0 33 27556.0 34 31545.0 35 35502.0 36 39252.0 37 42116.5 38 42184.5 39 40376.5 40 39100.5 41 38131.0 42 37734.0 43 38436.0 44 39286.5 45 39016.0 46 37991.0 47 37434.0 48 37257.5 49 35996.0 50 33783.5 51 31000.0 52 27595.5 53 24280.0 54 21261.5 55 18164.0 56 15180.0 57 12442.5 58 10040.5 59 7686.5 60 5493.5 61 3947.5 62 2828.5 63 1901.0 64 1225.0 65 868.0 66 616.5 67 444.5 68 334.0 69 242.0 70 193.0 71 156.5 72 133.0 73 121.5 74 92.5 75 73.0 76 58.5 77 34.0 78 24.0 79 20.5 80 13.0 81 8.5 82 6.5 83 3.5 84 2.5 85 1.5 86 1.5 87 1.5 88 2.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027303106025083245 2 0.012860158635002977 3 0.0013849401606926283 4 0.0 5 5.935457831539835E-4 6 0.0 7 0.0 8 9.89242971923306E-5 9 1.978485943846612E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013849401606926283 26-27 0.008359103112751936 28-29 2.473107429808265E-4 30-31 0.0 32-33 1.978485943846612E-4 34-35 0.009842967570636895 36-37 7.419322289424795E-4 38-39 0.01147521847431035 40-41 0.011277369879925687 42-43 0.004352669076462546 44-45 5.935457831539835E-4 46-47 9.89242971923306E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 9.89242971923306E-5 66-67 2.473107429808265E-4 68-69 8.408565261348102E-4 70-71 9.89242971923306E-5 72-73 1.4838644578849588E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.89242971923306E-5 82-83 0.0010387051205194711 84-85 0.005094601305405026 86-87 0.0033139639559430748 88-89 0.0 90-91 0.0013849401606926283 92-93 4.94621485961653E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 9.89242971923306E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1010874.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.31699163850811 #Duplication Level Percentage of deduplicated Percentage of total 1 75.42368683546864 43.98482514529478 2 14.214782850946467 16.57926745323707 3 4.945208705690932 8.651690842213668 4 2.18733528537118 5.102352541904194 5 1.0702203433518698 3.1206015407306134 6 0.6316642129268597 2.2102053974160287 7 0.3508319492925956 1.4321624697392459 8 0.2309145022757637 1.077299127874078 9 0.15825783375593414 0.8306208691085896 >10 0.6817614408623742 7.116229007080041 >50 0.062261275812576844 2.468748421810795 >100 0.04053097951856337 4.228154656352754 >500 0.001187099538912776 0.48825571538502877 >1k 0.0010175138904966652 1.3934622300536876 >5k 3.391712968322217E-4 1.3161245817994658 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7105 0.7028571315515089 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6203 0.6136274154840267 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4233 0.4187465500151354 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3226 0.3191297827424585 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 2503 0.24760751587240346 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1571 0.15541007088915137 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1534 0.15174987189303513 RNA PCR Primer, Index 33 (95% over 22bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1023 0.1011995560277542 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 9.89242971923306E-5 0.0 9 0.0 0.0 0.0 1.978485943846612E-4 0.0 10-11 0.0 0.0 0.0 1.978485943846612E-4 0.0 12-13 0.0 0.0 0.0 2.4731074298082654E-4 0.0 14-15 0.0 0.0 0.0 2.967728915769918E-4 0.0 16-17 0.0 0.0 0.0 3.462350401731571E-4 0.0 18-19 0.0 0.0 0.0 6.43007931750149E-4 0.0 20-21 0.0 0.0 0.0 0.0010387051205194711 0.0 22-23 0.0 0.0 0.0 0.0030171910643660834 0.0 24-25 0.0 0.0 0.0 0.006331155020309159 0.0 26-27 0.0 0.0 0.0 0.01231607500044516 0.0 28-29 0.0 0.0 0.0 0.028688046185775876 0.0 30-31 0.0 0.0 0.0 0.0722641990989975 0.0 32-33 0.0 0.0 0.0 0.14922730231463072 0.0 34-35 0.0 0.0 0.0 0.2755041676806407 0.0 36-37 0.0 0.0 0.0 0.4659829019244733 0.0 38-39 0.0 0.0 0.0 0.6958829685994496 0.0 40-41 0.0 0.0 0.0 0.9368130944113708 0.0 42-43 0.0 0.0 0.0 1.1725496946206946 0.0 44-45 0.0 0.0 0.0 1.4624968096914155 0.0 46-47 0.0 0.0 0.0 1.795723304783781 0.0 48-49 0.0 0.0 0.0 2.13819922166363 0.0 50-51 0.0 0.0 0.0 2.4769654773987657 0.0 52-53 0.0 0.0 0.0 2.800645777812072 0.0 54-55 0.0 0.0 0.0 3.1740849997131195 0.0 56-57 0.0 0.0 0.0 3.6124185605723365 0.0 58-59 0.0 0.0 0.0 4.071377837396154 0.0 60-61 0.0 0.0 0.0 4.539438149561667 0.0 62-63 0.0 0.0 0.0 4.985883502790655 0.0 64-65 0.0 0.0 0.0 5.470810407627459 0.0 66-67 0.0 0.0 0.0 6.024638085458722 0.0 68-69 0.0 0.0 0.0 6.638364425239941 0.0 70-71 0.0 0.0 0.0 7.296359388014729 0.0 72-73 0.0 0.0 0.0 7.961081202998594 0.0 74-75 0.0 0.0 0.0 8.61947186296215 0.0 76-77 0.0 0.0 0.0 9.339393435779336 0.0 78-79 0.0 0.0 0.0 10.137663051972847 0.0 80-81 0.0 0.0 0.0 10.978470115959063 0.0 82-83 0.0 0.0 0.0 11.808444969402714 0.0 84-85 0.0 0.0 0.0 12.61532099945196 0.0 86-87 0.0 0.0 0.0 13.46686134968354 0.0 88-89 0.0 0.0 0.0 14.366132673310423 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3230 0.0 56.18671 1 CCAGTCG 45 1.5159789E-5 52.77695 8 GTATCAA 5695 0.0 39.041355 1 ATCAACG 7250 0.0 30.465044 3 TCAACGC 7250 0.0 30.465044 4 TATCAAC 7295 0.0 30.345226 2 GTGGTAT 1300 0.0 29.967 1 CAACGCA 7435 0.0 29.770887 5 AACGCAG 7620 0.0 29.048101 6 CATTCCG 245 0.0 27.142431 9 CTCCGAC 295 0.0 25.76231 94-95 ACGCAGA 8600 0.0 25.627504 7 CGCAGAG 8680 0.0 25.336586 8 TCGTATC 75 1.0609219E-6 25.332937 94-95 TGGTATC 1545 0.0 24.29005 2 AGCCGAA 50 0.0016542292 23.749628 14-15 GCAGAGT 9080 0.0 23.488071 9 GAGTACT 6260 0.0 23.370241 12-13 GTACTTT 6315 0.0 22.640186 14-15 GTATAGC 705 0.0 22.238094 1 >>END_MODULE