FastQCFastQC Report
Thu 26 May 2016
SRR938667_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938667_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125758
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51500.2422665232825185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41280.19418955497286144No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29510.13882106994305088No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28300.1331289826969956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27230.12809548405792193No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25840.12155664003146172No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25490.1199101685140077No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23240.10932570875894622No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA21930.10316320107933265No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21670.1019401079520811No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21520.10123447730174366No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30000.057.0740931
GTATCAA58300.046.1748621
ATCAACG75150.035.1878973
TATCAAC76800.034.431912
TCAACGC76900.034.263444
CAACGCA78200.033.693845
AACGCAG80900.032.543256
TGGTATC13550.029.4840742
ACGCAGA93450.027.9198557
CGCAGAG95400.027.299358
TACATGG70950.024.467412
GTACATG73850.024.0223831
GCAGAGT106100.023.7399969
GTGGTAT19300.023.6576521
ACATGGG73850.022.2185693
GAGTACT62700.021.5996312-13
CAGAGTA97600.021.0353110-11
AGAGTAC94400.020.89253410-11
GTACTTT66450.019.98220414-15
AGTACTT66000.019.79966212-13