##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938667_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2125758 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.07879636346188 31.0 31.0 34.0 28.0 34.0 2 31.112243726708307 31.0 31.0 34.0 27.0 34.0 3 31.15461402473847 31.0 31.0 34.0 27.0 34.0 4 34.82031821119808 37.0 35.0 37.0 32.0 37.0 5 34.80044812250501 37.0 35.0 37.0 32.0 37.0 6 34.81288368666612 37.0 35.0 37.0 32.0 37.0 7 34.717319186850055 37.0 35.0 37.0 31.0 37.0 8 34.68663789575295 37.0 35.0 37.0 30.0 37.0 9 36.178490684264155 39.0 35.0 39.0 31.0 39.0 10-11 36.01180026136559 38.5 35.0 39.0 30.0 39.0 12-13 35.87003153698586 38.0 35.0 39.0 30.0 39.0 14-15 36.82306311442789 39.0 36.0 41.0 30.5 41.0 16-17 36.645151988137876 39.0 36.0 40.5 30.0 41.0 18-19 36.798944188378925 39.0 36.0 41.0 30.0 41.0 20-21 36.774073295266916 39.0 36.0 41.0 30.0 41.0 22-23 36.68249890156829 39.0 36.0 41.0 30.0 41.0 24-25 36.53519826810013 39.0 36.0 41.0 30.0 41.0 26-27 36.323882586823146 39.0 35.5 41.0 29.0 41.0 28-29 36.12487569140043 39.0 35.0 40.0 28.5 41.0 30-31 35.86164111813292 38.5 35.0 40.0 27.0 41.0 32-33 35.58705553501386 38.0 34.5 40.0 27.0 41.0 34-35 35.32554975683968 38.0 34.0 40.0 26.5 41.0 36-37 35.036595887208236 38.0 34.0 40.0 25.0 41.0 38-39 34.892359102023846 38.0 34.0 40.0 25.0 41.0 40-41 34.77791239642518 38.0 33.5 40.0 24.5 41.0 42-43 34.47538266350168 38.0 33.0 40.0 24.0 41.0 44-45 34.123042698181074 38.0 33.0 40.0 23.0 41.0 46-47 33.74087596048092 38.0 33.0 40.0 21.5 41.0 48-49 33.35788316449944 37.5 32.0 40.0 19.5 41.0 50-51 32.658092313424206 36.5 31.5 39.5 18.0 40.5 52-53 32.67703096965882 37.0 31.0 39.0 18.0 40.0 54-55 32.77370777859004 37.0 31.0 40.0 16.0 41.0 56-57 32.447277394698745 36.5 31.0 39.5 13.5 41.0 58-59 32.06428012972314 36.0 31.0 39.0 8.5 41.0 60-61 31.5836776810907 35.5 30.0 39.0 7.5 40.0 62-63 31.014741565126414 35.0 29.0 39.0 4.5 40.0 64-65 30.49119372948379 35.0 29.0 38.0 2.0 40.0 66-67 29.952052162099356 34.0 28.0 38.0 2.0 40.0 68-69 29.361931132330206 34.0 27.5 37.0 2.0 39.5 70-71 28.72628798762606 34.0 26.0 36.5 2.0 39.0 72-73 28.084878664457573 33.5 26.0 36.0 2.0 39.0 74-75 27.40638515767082 33.0 25.0 35.5 2.0 38.5 76-77 26.66464127148998 32.0 23.0 35.0 2.0 37.5 78-79 25.876217800897372 32.0 20.5 35.0 2.0 37.0 80-81 25.181331318052194 31.0 19.0 35.0 2.0 36.0 82-83 24.43052431179843 31.0 15.0 34.0 2.0 36.0 84-85 23.72177547961715 30.0 7.5 34.0 2.0 35.0 86-87 22.959963222530504 30.0 3.5 34.0 2.0 35.0 88-89 22.13102690898964 29.0 2.0 34.0 2.0 35.0 90-91 21.19462634034542 29.0 2.0 34.0 2.0 35.0 92-93 20.23393914076767 27.0 2.0 33.5 2.0 35.0 94-95 19.213012252570614 25.5 2.0 33.0 2.0 35.0 96-97 17.9425261483198 24.0 2.0 32.5 2.0 34.0 98-99 16.40410926361326 10.5 2.0 32.0 2.0 34.0 100-101 12.945794394281947 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.456072454465005 1101 2 0.35706228165098963 1101 3 0.40367097870142743 1101 4 0.31056145539162117 1101 5 0.2604738862363618 1101 6 0.3485831591237769 1101 7 0.43716090389573736 1101 8 0.5260063538620798 1101 9 0.5531193456788728 1101 10-11 0.6316606500723481 1101 12-13 0.7129956903968093 1101 14-15 0.8428671688712654 1101 16-17 0.6068988964606561 1101 18-19 0.8601137086679742 1101 20-21 0.8434595233484643 1101 22-23 0.7821067680552787 1101 24-25 0.6414057532997575 1101 26-27 0.7085070063420602 1101 28-29 0.782723718503135 1101 30-31 0.7954565517184662 1101 32-33 0.728571204589521 1101 34-35 0.6817345596276212 1101 36-37 0.7767895426439679 1101 38-39 0.7718510832021295 1101 40-41 0.8508801523220768 1101 42-43 0.9822475019871675 1101 44-45 1.0968006928516516 1101 46-47 0.8495064585580678 1101 48-49 0.7261908263732053 1101 50-51 0.7429673210280114 1101 52-53 0.7560981856477369 1101 54-55 0.8167782480376502 1101 56-57 0.7992135463110301 1101 58-59 0.7762493607283503 1101 60-61 0.8444516110483846 1101 62-63 0.543856046508953 1101 64-65 0.5365057024659059 1101 66-67 0.758675661664423 1101 68-69 0.6294813390568095 1101 70-71 0.7923305751925334 1101 72-73 0.9091275397910117 1101 74-75 0.8744722001277445 1101 76-77 0.8935753988792854 1101 78-79 1.2553089364240648 1101 80-81 1.1841911280378028 1101 82-83 0.9389138419705318 1101 84-85 0.8983705869894081 1101 86-87 1.0783263759896649 1101 88-89 1.2042473803634266 1101 90-91 0.8822986332518568 1101 92-93 0.6212087956210652 1101 94-95 0.6890401029387654 1101 96-97 0.4679512093337692 1101 98-99 0.6724226637894191 1101 100-101 0.5994518934320894 1102 1 0.5141022661992771 1102 2 0.43301349064750383 1102 3 0.3444945963495414 1102 4 0.42806877583856817 1102 5 0.39799140141618494 1102 6 0.4760249548339033 1102 7 0.5280464983339925 1102 8 0.5556548376762578 1102 9 0.6701533071634387 1102 10-11 0.7660585545382688 1102 12-13 0.7838185445627204 1102 14-15 0.8622298633346688 1102 16-17 0.8512242027517445 1102 18-19 0.7305217000810487 1102 20-21 0.7834988875923443 1102 22-23 0.9076298656372828 1102 24-25 0.9511649644879085 1102 26-27 0.7736387743871873 1102 28-29 0.8306733196959613 1102 30-31 0.9219210902159247 1102 32-33 0.9015913156088118 1102 34-35 0.8517342003418875 1102 36-37 1.0249458454140594 1102 38-39 1.1017128929244038 1102 40-41 1.2739542684791587 1102 42-43 1.3009231747497125 1102 44-45 1.2442347190076575 1102 46-47 1.1619161880159012 1102 48-49 1.3417751821738761 1102 50-51 1.3550481917569996 1102 52-53 1.2534684385848962 1102 54-55 1.394559231941649 1102 56-57 1.3603851995643623 1102 58-59 1.2604917618349418 1102 60-61 1.2493883093920815 1102 62-63 1.3910243134941815 1102 64-65 1.4722566545731155 1102 66-67 1.1226905001654863 1102 68-69 1.1524062393550167 1102 70-71 1.2373948873399847 1102 72-73 1.1002748514354614 1102 74-75 1.1289798888425828 1102 76-77 1.275243293105568 1102 78-79 1.4534375068261056 1102 80-81 1.4659183044111757 1102 82-83 1.525164762640241 1102 84-85 1.4514858189083526 1102 86-87 1.5242837377544767 1102 88-89 1.637509155953552 1102 90-91 1.63799005620303 1102 92-93 1.6426724134488246 1102 94-95 1.9554675451835806 1102 96-97 1.7693398487186727 1102 98-99 1.8622645331531231 1102 100-101 1.6352458475513014 1103 1 0.4402455851303344 1103 2 0.3853898227638126 1103 3 0.48517491084287556 1103 4 0.4073268435898214 1103 5 0.36991729564483933 1103 6 0.387174126842595 1103 7 0.325684469873309 1103 8 0.2839223636790038 1103 9 0.4749635558469052 1103 10-11 0.607154295051032 1103 12-13 0.5833510162539923 1103 14-15 0.7438767394706716 1103 16-17 0.8859786051776481 1103 18-19 0.9456116978120406 1103 20-21 0.7926438727089291 1103 22-23 0.7993012657443543 1103 24-25 0.7732559870785494 1103 26-27 0.7358685304723309 1103 28-29 0.7164530277360512 1103 30-31 0.773519412239402 1103 32-33 0.7717058558023098 1103 34-35 0.6884953349491809 1103 36-37 0.8152211869777446 1103 38-39 0.7871158981496436 1103 40-41 0.826215257656898 1103 42-43 1.0281756306819432 1103 44-45 0.8538308512121588 1103 46-47 0.5734540061199596 1103 48-49 0.7343969323887478 1103 50-51 1.000838881523869 1103 52-53 0.9779423958157665 1103 54-55 0.8568528378051283 1103 56-57 0.8611786989390211 1103 58-59 0.7887419711686761 1103 60-61 1.1853959295966305 1103 62-63 1.1151277897284722 1103 64-65 1.2670077894566951 1103 66-67 0.9942413884388124 1103 68-69 1.2339466484330401 1103 70-71 1.0826757795922326 1103 72-73 1.0646324258574396 1103 74-75 1.3226642211947102 1103 76-77 1.4210641130063308 1103 78-79 1.0327295770317093 1103 80-81 1.1444285792219233 1103 82-83 1.2626742993170978 1103 84-85 1.2643461432217187 1103 86-87 1.2547467389290077 1103 88-89 1.1421867232748255 1103 90-91 1.1119837260468124 1103 92-93 0.951684347374858 1103 94-95 1.284336760170678 1103 96-97 1.6065112752575956 1103 98-99 1.7821582966940106 1103 100-101 1.4127028294429813 1104 1 0.4551262830565044 1104 2 0.30035346535313323 1104 3 0.3553767215886765 1104 4 0.23833411081838562 1104 5 0.41743735655599323 1104 6 0.45118677696711984 1104 7 0.4408248483987265 1104 8 0.5403525955199697 1104 9 0.6685895775018409 1104 10-11 0.6961419022676409 1104 12-13 0.7137816053950488 1104 14-15 0.830943433360197 1104 16-17 0.8749507931940528 1104 18-19 0.9183537420364374 1104 20-21 0.9435506280200414 1104 22-23 0.7401645920805677 1104 24-25 0.7576673883542 1104 26-27 0.8374102378137849 1104 28-29 0.8271103535575719 1104 30-31 0.8234201976615552 1104 32-33 0.6776689390468462 1104 34-35 0.5458195613838583 1104 36-37 0.9622621892895609 1104 38-39 1.1430334681687597 1104 40-41 1.0351805123501805 1104 42-43 1.0262763042351466 1104 44-45 0.9769091403402328 1104 46-47 1.023408811912475 1104 48-49 1.0675732025586626 1104 50-51 1.176328095527488 1104 52-53 1.3021620247766421 1104 54-55 1.4120001921893888 1104 56-57 1.3788006116429585 1104 58-59 1.3707281981010198 1104 60-61 1.3240694104863948 1104 62-63 1.3913940250828922 1104 64-65 1.521206703519642 1104 66-67 1.5257550434698324 1104 68-69 1.6227483745327511 1104 70-71 1.8143801010091813 1104 72-73 1.5517063508201652 1104 74-75 1.6149138931379348 1104 76-77 1.638354991432852 1104 78-79 1.6432216515628078 1104 80-81 1.678461412547815 1104 82-83 1.5491537875272599 1104 84-85 1.7505988096799605 1104 86-87 1.7831801694564717 1104 88-89 1.8252256030539797 1104 90-91 1.579775143613638 1104 92-93 1.7984929982905413 1104 94-95 1.883095248706237 1104 96-97 1.900423987696957 1104 98-99 1.8247518699713652 1104 100-101 1.3122098403938036 1105 1 0.4888930813470189 1105 2 0.256891189398047 1105 3 0.2551817232949105 1105 4 0.301584244879713 1105 5 0.24997566559579099 1105 6 0.31746832200359876 1105 7 0.36957775306080975 1105 8 0.30826237630938635 1105 9 0.5605190856186439 1105 10-11 0.553764913691289 1105 12-13 0.624736040793735 1105 14-15 0.6148140276175056 1105 16-17 0.7080298162526049 1105 18-19 0.7585028751065224 1105 20-21 0.6028841401018141 1105 22-23 0.5638264162896078 1105 24-25 0.6860514212439099 1105 26-27 0.7764990676615255 1105 28-29 0.7253787280843014 1105 30-31 0.8410218405221812 1105 32-33 0.9309168793773637 1105 34-35 0.9432080061099626 1105 36-37 0.7489348684036159 1105 38-39 0.7487656055125527 1105 40-41 0.786288549529857 1105 42-43 0.8161203462247784 1105 44-45 0.9209777391149956 1105 46-47 0.8019051719443198 1105 48-49 0.8548684543502105 1105 50-51 0.8718297286381969 1105 52-53 0.8817808437369692 1105 54-55 1.0353323474080298 1105 56-57 0.8014283355253795 1105 58-59 0.9509123089900555 1105 60-61 0.9992841117327629 1105 62-63 1.1506304442642197 1105 64-65 1.3389814073659885 1105 66-67 1.449203994541513 1105 68-69 1.4029397978577975 1105 70-71 1.482133476917145 1105 72-73 1.359942794376284 1105 74-75 1.489367491668947 1105 76-77 1.4944582349044708 1105 78-79 1.3293781945680578 1105 80-81 1.4805229101347095 1105 82-83 1.5285995455018764 1105 84-85 1.4777009319113894 1105 86-87 1.7279859024063065 1105 88-89 1.5445430277765126 1105 90-91 1.410350063583092 1105 92-93 1.6046017260891752 1105 94-95 1.5547258406885582 1105 96-97 1.9414969251837562 1105 98-99 1.9509933591083346 1105 100-101 1.6308652865336501 1106 1 0.2413824304816501 1106 2 0.19383264104858355 1106 3 0.21211078224955315 1106 4 0.14552887506501833 1106 5 0.2826770040722124 1106 6 0.024511191430761414 1106 7 0.3791200881090475 1106 8 0.2867885021356358 1106 9 0.307560013333088 1106 10-11 0.17545043863087528 1106 12-13 0.24598029520053188 1106 14-15 0.5635773891301454 1106 16-17 0.288310255047044 1106 18-19 0.2145450078250306 1106 20-21 0.35528333828867886 1106 22-23 0.4026428444845749 1106 24-25 0.2412594945264388 1106 26-27 0.08613238989935468 1106 28-29 0.3140724734849698 1106 30-31 0.040312034383433115 1106 32-33 0.2958628051256227 1106 34-35 0.08236588611864448 1106 36-37 0.0747583422418927 1106 38-39 0.1206957821757797 1106 40-41 0.2861179358017836 1106 42-43 -0.2805042350424216 1106 44-45 -0.10681863314560047 1106 46-47 0.2792070193452858 1106 48-49 0.5669284687743641 1106 50-51 0.11895652411057966 1106 52-53 0.10260671208232708 1106 54-55 0.25729611651717477 1106 56-57 -0.45115799726460537 1106 58-59 -0.10187045904380909 1106 60-61 0.4701077365024844 1106 62-63 0.48540048184207407 1106 64-65 0.4585952961596931 1106 66-67 0.3630076547515735 1106 68-69 0.22092911354275557 1106 70-71 0.08155834650421312 1106 72-73 0.5114034232954019 1106 74-75 0.7949290300928276 1106 76-77 0.579850660756339 1106 78-79 0.877677380680403 1106 80-81 1.0489430943200304 1106 82-83 0.6498261572458119 1106 84-85 0.7528341799072642 1106 86-87 0.7005914361243946 1106 88-89 0.7944485936389185 1106 90-91 1.0943232646841565 1106 92-93 0.9500195696132359 1106 94-95 1.0019514350729288 1106 96-97 0.9874570817282233 1106 98-99 1.1474912191451132 1106 100-101 0.8014544372546126 1107 1 0.20358119572374633 1107 2 0.10583850542721507 1107 3 0.22670419548464338 1107 4 0.2691715952517555 1107 5 0.14264171840419237 1107 6 0.37279644583706073 1107 7 0.2732185962034279 1107 8 0.2311199902257215 1107 9 0.31819801700753914 1107 10-11 0.410790894827592 1107 12-13 0.4710376484387666 1107 14-15 0.48261804299713873 1107 16-17 0.54916288247464 1107 18-19 0.6492526946819623 1107 20-21 0.7937873205512531 1107 22-23 0.668885614209124 1107 24-25 0.7239887952577959 1107 26-27 0.5467937196287735 1107 28-29 0.5413775646569832 1107 30-31 0.654591167103078 1107 32-33 0.7271288968972129 1107 34-35 0.7921366575297171 1107 36-37 0.6596348223642465 1107 38-39 0.6731622720133146 1107 40-41 0.6888390684059971 1107 42-43 0.9010412082808728 1107 44-45 0.8760839096348647 1107 46-47 0.784952262753869 1107 48-49 0.8299714207787972 1107 50-51 0.8997636496993451 1107 52-53 1.087785248584801 1107 54-55 1.1157511501355515 1107 56-57 0.9403630218355019 1107 58-59 0.9354276824066687 1107 60-61 0.6242374502092289 1107 62-63 0.9250841933620997 1107 64-65 0.9980878702980718 1107 66-67 0.9251966406854031 1107 68-69 1.2134411292665988 1107 70-71 1.3480998059617626 1107 72-73 1.4007140782525518 1107 74-75 1.3901627595683088 1107 76-77 1.646591133145023 1107 78-79 1.61160264271777 1107 80-81 1.6610267923734696 1107 82-83 1.391907723089414 1107 84-85 1.4882744331432534 1107 86-87 1.4938858165491062 1107 88-89 1.7840550726711228 1107 90-91 1.5634830738112981 1107 92-93 1.5173407886280614 1107 94-95 1.4910942987429578 1107 96-97 1.6617012093337706 1107 98-99 1.6804646218313763 1107 100-101 1.1967202500754475 1108 1 0.2596924931021647 1108 2 0.14373246078692148 1108 3 0.1841438448763455 1108 4 0.10408753547591942 1108 5 0.11561087253772939 1108 6 0.15438312399908227 1108 7 0.14082484839872222 1108 8 0.2997133261135758 1108 9 0.24484528526439675 1108 10-11 0.3422606237288264 1108 12-13 0.3007679067649107 1108 14-15 0.5498932050497061 1108 16-17 0.5071425740159725 1108 18-19 0.5222350205752448 1108 20-21 0.491724144001779 1108 22-23 0.32304130440932965 1108 24-25 0.4581240093587624 1108 26-27 0.5047618359873027 1108 28-29 0.5017678878041494 1108 30-31 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1.4027070927197869 1108 98-99 1.4994094042179427 1108 100-101 1.3135797034075019 1109 1 0.3239961894752419 1109 2 0.17304648755915153 1109 3 0.25288310212932785 1109 4 0.32229551505818677 1109 5 0.3318617189359756 1109 6 0.24449936269206773 1109 7 0.2842598363321258 1109 8 0.3292730254034666 1109 9 0.48254863786957003 1109 10-11 0.38762822099951677 1109 12-13 0.3660777319294226 1109 14-15 0.3127094107548558 1109 16-17 0.36798949312634477 1109 18-19 0.25891726965071626 1109 20-21 0.248686746220649 1109 22-23 0.37083611143052764 1109 24-25 0.31966237073175563 1109 26-27 0.6004710309106471 1109 28-29 0.41068328270318943 1109 30-31 0.23464929813554392 1109 32-33 0.5848906693452989 1109 34-35 0.357915460475823 1109 36-37 0.358700371833784 1109 38-39 0.19445454912783333 1109 40-41 0.4677865942641688 1109 42-43 0.5572438459473901 1109 44-45 0.2701609590113421 1109 46-47 0.48771207423516927 1109 48-49 0.7623645979438365 1109 50-51 0.6582431692292445 1109 52-53 0.7661833713745523 1109 54-55 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-1.9297989815890872 2316 76-77 -2.198965710373777 2316 78-79 -2.3482619944566494 2316 80-81 -2.4705608229097997 2316 82-83 -2.6282161747518735 2316 84-85 -2.5821687106694533 2316 86-87 -2.3901658202199236 2316 88-89 -2.573891554976594 2316 90-91 -2.6729236354214976 2316 92-93 -2.8323760705996683 2316 94-95 -3.1089566919330345 2316 96-97 -3.168705702594883 2316 98-99 -3.133485920380034 2316 100-101 -2.854721740904587 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7848.0 3 2670.0 4 2883.0 5 3173.0 6 3806.0 7 4808.0 8 6153.0 9 7786.0 10 9713.0 11 12292.0 12 14832.0 13 17128.0 14 19531.0 15 22305.0 16 25486.0 17 29098.0 18 28145.0 19 27200.0 20 28746.0 21 31203.0 22 34423.0 23 38474.0 24 42534.0 25 47598.0 26 53823.0 27 60551.0 28 68801.0 29 78127.0 30 90061.0 31 104159.0 32 122927.0 33 145569.0 34 175304.0 35 208678.0 36 237819.0 37 217333.0 38 90802.0 39 3969.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.555210140645755 18.24438376683741 14.602629627817526 23.597776464699308 2 15.622327459867162 19.488963233382687 40.2993621694527 24.58934713729745 3 19.399490029963328 23.37408195502957 29.502152819576672 27.724275195430426 4 13.109665058068723 16.186520746768753 36.38590148522919 34.31791270993333 5 14.137393890505557 36.994330775145876 34.00258017580628 14.865695158542286 6 33.185481934348324 35.43776727436417 17.034317533951672 14.342433257335832 7 29.363237511222533 32.297001488809464 20.04340321891522 18.296357781052784 8 26.88766495467496 34.701875819038634 19.32608854204653 19.084370684239875 9 26.62228951831016 16.739206537716655 19.729982289292455 36.908521654680726 10-11 25.411890565399236 26.132821131796273 27.093552007368622 21.36173629543586 12-13 26.353770563253466 23.376367255435486 27.25118528026177 23.01867690104928 14-15 22.88949385967203 24.9841953931718 25.521210876392797 26.60509987076337 16-17 22.698252217756217 27.98684463459815 26.44091508751852 22.873988060127115 18-19 22.778904727360686 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27.892002208857498 23.158016837479803 44-45 22.753608427116017 26.391544656850996 28.17434675949002 22.68050015654297 46-47 23.233638185228696 26.708728669267135 27.30677252973753 22.75086061576664 48-49 22.904410156141573 26.751470546032103 27.308638478789938 23.03548081903639 50-51 23.03429395809188 26.89940644393985 27.323832076773453 22.74246752119482 52-53 23.40945145646598 26.462323269770355 27.364346081684037 22.763879192079624 54-55 22.794381766357642 26.79206518238747 27.490898210269517 22.922654840985377 56-57 22.89483812020517 26.93152936814797 27.281711398817954 22.891921112828904 58-59 22.844527128806693 26.436591782582784 27.421864101067516 23.297016987543 60-61 22.452469210739046 26.692903530589756 27.725344829370986 23.129282429300215 62-63 22.668957215924102 26.624059564944623 27.85153270066437 22.855450518466906 64-65 22.454475826294452 26.68907316329613 27.58871250777437 23.26773850263505 66-67 22.326176697725984 26.88124262987128 27.49207090002077 23.30050977238196 68-69 23.0112394016787 26.317710990779403 27.657316690766727 23.01373291677517 70-71 22.748548551186335 26.358771607035468 27.317447784075988 23.57523205770221 72-73 22.706679848276597 26.543008296226724 27.35366789589414 23.396643959602535 74-75 22.757849142590945 26.96124160501423 27.128076690966886 23.152832561427942 76-77 22.892908110763393 26.498115313367087 27.289396679130867 23.319579896738652 78-79 22.9441390099689 26.80698540524228 27.081817627979632 23.167057956809188 80-81 23.244581332583138 26.700188788334543 26.815682274831214 23.2395476042511 82-83 23.125939861562053 26.773608299220164 26.931089873640733 23.169361965577046 84-85 22.993995045777517 26.927476458504803 26.9507162829077 23.12781221280999 86-87 23.230768760293135 26.779734618548567 26.761009669930434 23.22848695122786 88-89 23.120340823153988 26.79754048639677 26.556313891988275 23.52580479846097 90-91 23.018418113313384 26.861617209031248 26.63957020245407 23.4803944752013 92-93 23.270524892724666 26.43725892753368 26.82325720811674 23.468958971624915 94-95 22.987713778165585 26.244264990444886 27.06635068078861 23.70167055060092 96-97 23.437973924602208 25.750461262101588 27.084891757238598 23.726673056057614 98-99 23.075777491741427 26.090530583839616 26.64099240759349 24.19269951682547 100-101 23.88229492646081 25.386738348301662 26.944778229057185 23.78618849618034 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 183.0 1 131.5 2 124.5 3 178.5 4 173.0 5 176.5 6 287.0 7 616.5 8 1033.5 9 1190.5 10 1099.5 11 998.0 12 1003.5 13 1099.5 14 1198.5 15 1358.5 16 1565.0 17 1805.0 18 2060.5 19 2343.5 20 2737.0 21 3245.0 22 3937.0 23 4974.5 24 6298.5 25 8040.5 26 10736.0 27 14074.5 28 16863.0 29 19651.0 30 22885.0 31 27829.5 32 34476.0 33 37342.0 34 38130.0 35 40929.5 36 45136.0 37 49215.5 38 52543.0 39 55484.5 40 58427.0 41 62493.0 42 65443.5 43 67416.5 44 69482.5 45 73136.0 46 75858.5 47 77211.5 48 78700.5 49 78129.0 50 76595.5 51 75435.5 52 74967.5 53 75571.5 54 76737.0 55 73775.5 56 68200.5 57 62913.5 58 58976.0 59 53221.0 60 43951.5 61 36866.5 62 31328.0 63 24336.5 64 17562.0 65 13064.0 66 9783.5 67 7574.5 68 6012.5 69 4763.5 70 3800.5 71 2748.0 72 2071.5 73 1625.0 74 1235.0 75 978.0 76 728.0 77 526.5 78 388.5 79 242.5 80 140.5 81 89.5 82 52.5 83 38.5 84 29.0 85 14.5 86 10.5 87 9.5 88 8.0 89 5.5 90 4.0 91 4.0 92 4.0 93 3.0 94 2.5 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006303633809681064 2 0.10866712015196463 3 0.11741694021614878 4 0.12155664003146172 5 0.1609778723636463 6 0.094272254885081 7 0.026813964712822436 8 0.026014249975773344 9 0.02305060124435613 10-11 0.050499633542482256 12-13 0.054333560075982314 14-15 0.027590158428193616 16-17 0.0326236570672673 18-19 0.037563071619629324 20-21 0.058426217847939416 22-23 0.03234140480713232 24-25 0.04904133019845156 26-27 0.0570855196122983 28-29 0.053698492490678616 30-31 0.06745829017225856 32-33 0.05009977617395771 34-35 0.06221310233808364 36-37 0.05238131527671541 38-39 0.03165929517847281 40-41 0.03610476827559863 42-43 0.03187098437357404 44-45 0.006750533221561438 46-47 0.011525300622178065 48-49 0.008796862107539993 50-51 0.008444046782371276 52-53 0.009502492757877425 54-55 0.010090518299825286 56-57 0.013736276659902022 58-59 0.019122590624144422 60-61 0.023709189851337735 62-63 0.009996434213113628 64-65 0.008655735977472505 66-67 0.009478971736199512 68-69 0.011501779600500152 70-71 0.030106907747730456 72-73 0.027049174929601585 74-75 0.02848395725195436 76-77 0.022156802420595385 78-79 0.004586599227193312 80-81 0.004563078205515397 82-83 0.005292229877530744 84-85 0.004539557183837483 86-87 0.011831073903990954 88-89 0.03692800403432564 90-91 0.035634347842040344 92-93 0.02911902483725805 94-95 0.04607768146703435 96-97 0.04146756121816312 98-99 0.025520308520537147 100-101 0.07293868822321262 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2125758.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.373943374023106 #Duplication Level Percentage of deduplicated Percentage of total 1 76.56167178495379 41.62960006255619 2 12.930115727925545 14.06122760819578 3 4.428722830591755 7.22421373229518 4 2.0083051607788 4.367978843597793 5 1.0955668138001387 2.9785143948013824 6 0.6510850716380857 2.1241237690152626 7 0.438726413593353 1.669869961858925 8 0.3015333051052797 1.3116443885741207 9 0.22669878899032073 1.1093856403967381 >10 1.2260296027075677 12.124123740570948 >50 0.0836638182520478 3.132423413667528 >100 0.04172469611268207 4.131105183856774 >500 0.003294752829981206 1.2434334376202905 >1k 0.002774528698931519 2.6495621849363977 >5k 8.670402184160997E-5 0.24279363805666176 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5150 0.2422665232825185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4128 0.19418955497286144 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2951 0.13882106994305088 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2830 0.1331289826969956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2723 0.12809548405792193 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2584 0.12155664003146172 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2549 0.1199101685140077 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2324 0.10932570875894622 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2193 0.10316320107933265 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2167 0.1019401079520811 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2152 0.10123447730174366 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.7042043355828836E-5 0.0 5 0.0 0.0 0.0 4.7042043355828836E-5 4.7042043355828836E-5 6 0.0 0.0 0.0 4.7042043355828836E-5 4.7042043355828836E-5 7 0.0 0.0 0.0 4.7042043355828836E-5 4.7042043355828836E-5 8 0.0 0.0 0.0 4.7042043355828836E-5 4.7042043355828836E-5 9 0.0 0.0 0.0 4.7042043355828836E-5 4.7042043355828836E-5 10-11 0.0 0.0 0.0 4.7042043355828836E-5 4.7042043355828836E-5 12-13 0.0 0.0 0.0 9.408408671165767E-5 1.176051083895721E-4 14-15 0.0 0.0 0.0 9.408408671165767E-5 1.411261300674865E-4 16-17 2.3521021677914418E-5 0.0 0.0 9.408408671165767E-5 1.8816817342331535E-4 18-19 4.7042043355828836E-5 0.0 0.0 9.408408671165767E-5 2.5873123845705857E-4 20-21 4.7042043355828836E-5 0.0 0.0 9.408408671165767E-5 4.704204335582884E-4 22-23 4.7042043355828836E-5 0.0 0.0 3.0577328181288744E-4 5.174624769141171E-4 24-25 4.7042043355828836E-5 0.0 0.0 4.704204335582884E-4 5.64504520269946E-4 26-27 4.7042043355828836E-5 0.0 0.0 9.173198454386624E-4 6.115465636257749E-4 28-29 4.7042043355828836E-5 0.0 0.0 0.003010690774773046 6.115465636257749E-4 30-31 7.056306503374325E-5 0.0 0.0 0.01117248529700935 6.115465636257749E-4 32-33 9.408408671165767E-5 0.0 0.0 0.028342831121886874 6.115465636257749E-4 34-35 9.408408671165767E-5 0.0 0.0 0.053157508992086586 6.115465636257749E-4 36-37 9.408408671165767E-5 0.0 0.0 0.09486028042702885 6.115465636257749E-4 38-39 1.411261300674865E-4 0.0 0.0 0.16024872069163093 6.585886069816037E-4 40-41 1.411261300674865E-4 0.0 0.0 0.23107051696383124 6.585886069816037E-4 42-43 1.411261300674865E-4 0.0 0.0 0.30673764370168194 6.585886069816037E-4 44-45 1.411261300674865E-4 0.0 0.0 0.4036207319930114 7.526726936932615E-4 46-47 1.411261300674865E-4 0.0 0.0 0.5152044588330374 9.173198454386623E-4 48-49 1.411261300674865E-4 0.0 0.0 0.6366199727344317 9.878829104724056E-4 50-51 1.411261300674865E-4 0.0 0.0 0.7655857345944364 0.0010349249538282345 52-53 1.411261300674865E-4 0.0 0.0 0.892128831221616 0.001129009040539892 54-55 1.411261300674865E-4 0.0 0.0 1.0366655094324 0.0011995721055736352 56-57 1.411261300674865E-4 0.0 0.0 1.2052877138413687 0.0012701351706073786 58-59 1.411261300674865E-4 0.0 0.0 1.3875756318452053 0.0013642192573190364 60-61 1.411261300674865E-4 0.0 0.0 1.5832940532271311 0.0013642192573190364 62-63 1.411261300674865E-4 0.0 0.0 1.7814116188202043 0.0014112613006748652 64-65 1.411261300674865E-4 0.0 0.0 1.9827515643831517 0.0014112613006748652 66-67 1.411261300674865E-4 0.0 0.0 2.220149236178342 0.0014347823223527796 68-69 1.8816817342331535E-4 0.0 0.0 2.4873010003960943 0.001458303344030694 70-71 1.8816817342331535E-4 0.0 0.0 2.766542569756294 0.0014818243657086084 72-73 1.8816817342331535E-4 0.0 0.0 3.056227472741488 0.0016935135608098382 74-75 1.8816817342331535E-4 0.0 0.0 3.3490171505881667 0.0017405556041656671 76-77 1.8816817342331535E-4 0.0 0.0 3.673301476461573 0.0017875976475214958 78-79 1.8816817342331535E-4 0.0 0.0 4.039923641355225 0.0017875976475214958 80-81 1.8816817342331535E-4 0.0 0.0 4.417859417675954 0.0018111186691994103 82-83 1.8816817342331535E-4 0.0 0.0 4.814776658490759 0.0018346396908773246 84-85 1.8816817342331535E-4 0.0 0.0 5.216845003053029 0.0018346396908773246 86-87 1.8816817342331535E-4 0.0 0.0 5.635283978703127 0.0019287237775889824 88-89 1.8816817342331535E-4 0.0 0.0 6.067812046338294 0.0019287237775889824 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3000 0.0 57.074093 1 GTATCAA 5830 0.0 46.174862 1 ATCAACG 7515 0.0 35.187897 3 TATCAAC 7680 0.0 34.43191 2 TCAACGC 7690 0.0 34.26344 4 CAACGCA 7820 0.0 33.69384 5 AACGCAG 8090 0.0 32.54325 6 TGGTATC 1355 0.0 29.484074 2 ACGCAGA 9345 0.0 27.919855 7 CGCAGAG 9540 0.0 27.29935 8 TACATGG 7095 0.0 24.46741 2 GTACATG 7385 0.0 24.022383 1 GCAGAGT 10610 0.0 23.739996 9 GTGGTAT 1930 0.0 23.657652 1 ACATGGG 7385 0.0 22.218569 3 GAGTACT 6270 0.0 21.59963 12-13 CAGAGTA 9760 0.0 21.03531 10-11 AGAGTAC 9440 0.0 20.892534 10-11 GTACTTT 6645 0.0 19.982204 14-15 AGTACTT 6600 0.0 19.799662 12-13 >>END_MODULE