FastQCFastQC Report
Thu 26 May 2016
SRR938667_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938667_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125758
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51710.2432544061929909No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44500.2093370929334383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29690.13966782672345582No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28470.1339286974340447No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28170.13251743613336983No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA27880.1311532168760508No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27070.12734281136422865No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24750.11642905730567639No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG23380.10998429736592781No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21260.10001138417449211No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29250.054.9173741
GTATCAA53550.044.817831
TATCAAC74700.032.2556842
ATCAACG75550.031.6270163
TCAACGC77000.031.0931364
CAACGCA78550.030.5400585
AACGCAG81450.029.4388356
TGGTATC14150.027.8766482
TAAGGTG14350.027.4758385
ACGCAGA92900.025.7082567
AGGTAAG14550.025.4771332
CGCAGAG94300.025.3265868
TACCGTG1701.12811904E-725.1370287
GTAAGGT15050.024.9353394
TACATGG70800.024.2993262
GTACATG73700.024.1169871
GGTAAGG15100.023.9089973
GTGGTAT18600.023.5068171
AAGGTAA15000.023.4454941
ACATGGG73050.022.7600143
Produced by FastQC (version 0.11.5)