FastQCFastQC Report
Thu 26 May 2016
SRR938666_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938666_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133151
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.2479899453906451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40700.19079755722871938No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29690.13918377086291595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28010.1313080977389786No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27670.12971421151151513No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25750.12071344222701534No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25690.12043216818687472No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23320.1093218436013203No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA21860.10247750862456524No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29700.058.347451
GTATCAA58650.046.593021
ATCAACG77550.034.8700683
TATCAAC77750.034.718432
TCAACGC78950.034.1915324
CAACGCA79350.034.1365555
AACGCAG82250.032.9860886
ACGCAGA93600.028.7777187
CGCAGAG94650.028.2075188
TGGTATC12500.024.7124312
GCAGAGT106100.024.4912459
GTACATG74000.023.9325081
TACATGG72550.023.5818142
AGGTAAG12350.022.3189182
ACATGGG72800.022.2610343
AGAGTAC94100.021.90880610-11
GGTAAGG13450.021.9074443
TAAGGTG13950.021.802065
CAGAGTA97650.021.404210-11
GTAAGGT13400.021.279864
Produced by FastQC (version 0.11.5)