##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938666_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2133151 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.02374093535807 31.0 31.0 34.0 28.0 34.0 2 31.116467141801024 31.0 31.0 34.0 27.0 34.0 3 31.179448149709046 31.0 31.0 34.0 27.0 34.0 4 34.82578636017797 37.0 35.0 37.0 32.0 37.0 5 34.83625209842154 37.0 35.0 37.0 32.0 37.0 6 34.8633753541123 37.0 35.0 37.0 32.0 37.0 7 34.782297643251695 37.0 35.0 37.0 32.0 37.0 8 34.74414375728676 37.0 35.0 37.0 31.0 37.0 9 36.24446089376701 39.0 35.0 39.0 32.0 39.0 10-11 36.097017745110406 39.0 35.0 39.0 31.0 39.0 12-13 35.949587722575664 38.0 35.0 39.0 30.0 39.0 14-15 36.930957067736884 39.0 36.0 41.0 31.0 41.0 16-17 36.778215653744155 39.0 36.0 41.0 30.0 41.0 18-19 36.771149346670725 39.0 36.0 41.0 30.0 41.0 20-21 36.85096554346129 39.0 36.0 41.0 30.0 41.0 22-23 36.76727737511315 39.0 36.0 41.0 30.0 41.0 24-25 36.64455868337497 39.0 36.0 41.0 30.0 41.0 26-27 36.45811079478199 39.0 36.0 41.0 30.0 41.0 28-29 36.276060391411576 39.0 35.0 40.5 29.0 41.0 30-31 36.051016313425535 39.0 35.0 40.0 28.5 41.0 32-33 35.7701037104265 38.0 35.0 40.0 27.0 41.0 34-35 35.514731962247396 38.0 34.0 40.0 27.0 41.0 36-37 35.21830475198427 38.0 34.0 40.0 25.5 41.0 38-39 34.95381269305361 38.0 34.0 40.0 25.0 41.0 40-41 34.87191811550143 38.0 34.0 40.0 25.0 41.0 42-43 34.682433404855075 38.0 33.0 40.0 24.0 41.0 44-45 34.38349629257375 38.0 33.0 40.0 24.0 41.0 46-47 34.03099663361853 38.0 33.0 40.0 23.0 41.0 48-49 33.655845273025676 38.0 33.0 40.0 21.0 41.0 50-51 32.91080143881047 37.0 31.5 39.5 19.0 40.5 52-53 32.967787090552896 37.0 31.5 39.5 19.0 40.0 54-55 33.08858655575719 37.0 32.0 40.0 18.5 41.0 56-57 32.78143553831866 37.0 31.5 40.0 16.0 41.0 58-59 32.444344071282345 36.5 31.0 39.5 13.5 41.0 60-61 31.99400628459964 36.0 31.0 39.0 9.5 41.0 62-63 31.464926533564665 35.0 30.0 39.0 7.5 40.0 64-65 30.97489699510255 35.0 29.0 38.5 6.5 40.0 66-67 30.43181542234938 35.0 29.0 38.0 2.0 40.0 68-69 29.869972167933728 34.0 28.0 37.5 2.0 40.0 70-71 29.242644801047838 34.0 27.5 37.0 2.0 39.0 72-73 28.694744300801958 34.0 26.0 36.0 2.0 39.0 74-75 28.092117482541084 33.5 26.0 36.0 2.0 38.5 76-77 27.36728764161562 33.0 25.5 35.0 2.0 37.5 78-79 26.59298990085559 32.0 23.5 35.0 2.0 37.0 80-81 25.9534629756637 32.0 21.5 35.0 2.0 36.0 82-83 25.292827605734427 31.0 19.5 34.5 2.0 36.0 84-85 24.640780001040714 31.0 17.5 34.0 2.0 35.5 86-87 23.982866426239866 31.0 9.5 34.0 2.0 35.0 88-89 23.18751532357531 30.0 4.5 34.0 2.0 35.0 90-91 22.353473570319213 29.0 2.0 34.0 2.0 35.0 92-93 21.510996877389363 29.0 2.0 34.0 2.0 35.0 94-95 20.54789839069058 28.0 2.0 34.0 2.0 35.0 96-97 19.312223091567358 26.0 2.0 33.0 2.0 35.0 98-99 17.837524863453176 24.0 2.0 33.0 2.0 34.0 100-101 14.451877527657441 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6862470883980265 1101 2 0.627201893000148 1101 3 0.6292237041226585 1101 4 0.5328024287845565 1101 5 0.5712719184893444 1101 6 0.5393319903583134 1101 7 0.5529089822129762 1101 8 0.7179958959203177 1101 9 0.7648163554002849 1101 10-11 0.7521991926670566 1101 12-13 0.6608849237566332 1101 14-15 0.7669691603383058 1101 16-17 0.7963606405301391 1101 18-19 0.7542089155233853 1101 20-21 0.7932605763268725 1101 22-23 0.8485488855614491 1101 24-25 0.7868261643329646 1101 26-27 0.9267036804391466 1101 28-29 0.995890602183529 1101 30-31 0.9182063770594127 1101 32-33 0.9449378840700149 1101 34-35 0.828554277830456 1101 36-37 0.9153722906174906 1101 38-39 0.8624330414046071 1101 40-41 0.8298390328885503 1101 42-43 1.0056876163649235 1101 44-45 0.9424203903860402 1101 46-47 1.0768595635906664 1101 48-49 1.0124200679297957 1101 50-51 0.9804785629780568 1101 52-53 1.1323260729709261 1101 54-55 1.2091851923763528 1101 56-57 1.0661548332587145 1101 58-59 1.1771770905624095 1101 60-61 1.0771216104594643 1101 62-63 1.1215701238936227 1101 64-65 1.1354948707196861 1101 66-67 1.0867923074075705 1101 68-69 1.1888984921875725 1101 70-71 1.3838188084189973 1101 72-73 1.3134657564932581 1101 74-75 1.0376660571694423 1101 76-77 1.3019434904696041 1101 78-79 1.3525013177556033 1101 80-81 1.3724665277990802 1101 82-83 1.3124163910345565 1101 84-85 1.4937049269533453 1101 86-87 1.7482252374338323 1101 88-89 1.7908403801649477 1101 90-91 1.885897799831124 1101 92-93 1.7590652482854274 1101 94-95 1.463632578549369 1101 96-97 1.388333370879426 1101 98-99 1.748952991107469 1101 100-101 1.4406980665698317 1102 1 0.5196010682362662 1102 2 0.6068731647350809 1102 3 0.6683923681142581 1102 4 0.5231798109872656 1102 5 0.4828707658757665 1102 6 0.4814728533822148 1102 7 0.40100681073926125 1102 8 0.5049778257401485 1102 9 0.6939499203335444 1102 10-11 0.6298789743526996 1102 12-13 0.6948816382693295 1102 14-15 0.8077193017219741 1102 16-17 0.755892144403596 1102 18-19 0.8561425055915208 1102 20-21 0.5800492817910197 1102 22-23 0.758290445180478 1102 24-25 0.5721128365652177 1102 26-27 0.523628473300846 1102 28-29 0.7005854378642837 1102 30-31 0.7285511736913364 1102 32-33 0.7363027078117028 1102 34-35 0.7329432310526656 1102 36-37 0.816193886861619 1102 38-39 0.9733130762343691 1102 40-41 0.8111312796771202 1102 42-43 1.0181747122993556 1102 44-45 0.9747905193818198 1102 46-47 1.1368145452332286 1102 48-49 0.7064691617277674 1102 50-51 1.035786061250036 1102 52-53 0.9216235531996517 1102 54-55 0.9249777488624957 1102 56-57 0.9777741027315088 1102 58-59 1.0538716748944026 1102 60-61 1.1524752940652476 1102 62-63 0.9440472107615498 1102 64-65 0.9812161069964951 1102 66-67 0.9570788449886969 1102 68-69 1.1577599720931353 1102 70-71 1.2349988010580653 1102 72-73 1.140106704796203 1102 74-75 1.3728494968771585 1102 76-77 1.4383439518741064 1102 78-79 1.224391101326372 1102 80-81 1.4095357564277045 1102 82-83 1.3048296981558067 1102 84-85 1.1347012554661688 1102 86-87 1.1875929606629434 1102 88-89 1.2605963923374084 1102 90-91 1.5725083169811853 1102 92-93 1.6282887152824408 1102 94-95 1.6239953286638205 1102 96-97 1.6790366087900033 1102 98-99 1.6794622416393423 1102 100-101 1.67498540308093 1103 1 0.5625391436159184 1103 2 0.6129069465694776 1103 3 0.5128123329685614 1103 4 0.5524071302098648 1103 5 0.5551922178651694 1103 6 0.610812953573685 1103 7 0.6383386228409194 1103 8 0.5419594922756801 1103 9 0.5558220844167394 1103 10-11 0.5642476632083699 1103 12-13 0.6524943536678691 1103 14-15 0.7780893806686535 1103 16-17 0.8504858706343583 1103 18-19 0.7008745852201272 1103 20-21 0.7174637125094065 1103 22-23 0.8472621712538597 1103 24-25 0.939599788069394 1103 26-27 0.8046340598930186 1103 28-29 0.8383021478073616 1103 30-31 0.9992110734998718 1103 32-33 0.9978266934386895 1103 34-35 0.9553492687687211 1103 36-37 1.0219830913623653 1103 38-39 0.9107616035247403 1103 40-41 0.9749377685170515 1103 42-43 0.7933169999170531 1103 44-45 0.9796656734720912 1103 46-47 0.9046850831722182 1103 48-49 0.985726643302506 1103 50-51 1.0681620677546135 1103 52-53 1.094106963905972 1103 54-55 1.0135409717753916 1103 56-57 0.8270823357422401 1103 58-59 0.8524212591785059 1103 60-61 1.0295643648025177 1103 62-63 1.1593539487687359 1103 64-65 0.9987168282701333 1103 66-67 1.0293432507903617 1103 68-69 1.16728471833596 1103 70-71 1.2971745557725747 1103 72-73 1.299900289395044 1103 74-75 1.1064654383359667 1103 76-77 1.0687994033914023 1103 78-79 1.222674470067684 1103 80-81 1.2053418780737815 1103 82-83 1.085866102803429 1103 84-85 1.0739391970823462 1103 86-87 1.051760742025234 1103 88-89 1.1869432069307422 1103 90-91 1.2295577832386932 1103 92-93 1.3192065687720955 1103 94-95 1.127323022948712 1103 96-97 1.1535749936616178 1103 98-99 1.4770984312162838 1103 100-101 1.139105318101782 1104 1 0.4125857416172849 1104 2 0.4208503984736218 1104 3 0.42232521589474104 1104 4 0.40659457648435193 1104 5 0.4060665548557125 1104 6 0.3991499303366979 1104 7 0.508912028043703 1104 8 0.3957675222913366 1104 9 0.4595898902796449 1104 10-11 0.4089869555733614 1104 12-13 0.49250903074630514 1104 14-15 0.5459852526542122 1104 16-17 0.527142899008318 1104 18-19 0.5018024969922905 1104 20-21 0.6314245081724295 1104 22-23 0.6543630262109019 1104 24-25 0.6963183843756653 1104 26-27 0.6402180508524964 1104 28-29 0.7086565596303345 1104 30-31 0.649588250298379 1104 32-33 0.640303958609735 1104 34-35 0.6804018960863445 1104 36-37 0.6541558243825207 1104 38-39 0.6749386793752663 1104 40-41 0.6327831148323568 1104 42-43 0.7484188350686622 1104 44-45 0.7980534084646962 1104 46-47 0.9321262241255752 1104 48-49 0.8883234698149636 1104 50-51 0.632157661185758 1104 52-53 0.7646140896964653 1104 54-55 1.008015103424654 1104 56-57 0.8289163245483806 1104 58-59 0.7886621010777759 1104 60-61 0.7457426999188073 1104 62-63 0.902016270882374 1104 64-65 0.8216655256794212 1104 66-67 0.6485981737270841 1104 68-69 0.579609415965745 1104 70-71 0.6313836472924272 1104 72-73 0.6025276070211447 1104 74-75 0.46787019150437104 1104 76-77 0.5986632528169835 1104 78-79 0.6056997053402178 1104 80-81 0.6709842500412506 1104 82-83 0.5937935770194898 1104 84-85 0.5296919830571198 1104 86-87 0.42306003383897206 1104 88-89 0.34237187409009096 1104 90-91 0.5051055035542191 1104 92-93 0.42903723973163466 1104 94-95 0.4650820897483676 1104 96-97 0.35944750463066555 1104 98-99 0.28130521436933975 1104 100-101 0.22809328875451662 1105 1 0.403042143364047 1105 2 0.3733691101042673 1105 3 0.4194088885227387 1105 4 0.37586606896380204 1105 5 0.34483947391112224 1105 6 0.2766395668931452 1105 7 0.27892234533634763 1105 8 0.3002371867844644 1105 9 0.43498653172398605 1105 10-11 0.5491199932673752 1105 12-13 0.47240107863659375 1105 14-15 0.6330917135767038 1105 16-17 0.6447296781054419 1105 18-19 0.6233281025435176 1105 20-21 0.5259910075334915 1105 22-23 0.5641886578117692 1105 24-25 0.576652045209535 1105 26-27 0.6973165738182132 1105 28-29 0.5155136620504877 1105 30-31 0.520524149759126 1105 32-33 0.7067065053195876 1105 34-35 0.5794330346190648 1105 36-37 0.6369354835221444 1105 38-39 0.6954350170440406 1105 40-41 0.6752860531070581 1105 42-43 0.7034224143282302 1105 44-45 0.4872807601613047 1105 46-47 0.5764484313288065 1105 48-49 0.5522719161701701 1105 50-51 0.37625065219555864 1105 52-53 0.4519338703873359 1105 54-55 0.37382538010449906 1105 56-57 0.6212148655642338 1105 58-59 0.5649597108707951 1105 60-61 0.6363239142415864 1105 62-63 0.5324152608953092 1105 64-65 0.6360311762739457 1105 66-67 0.6040730050711467 1105 68-69 0.4842540436919833 1105 70-71 0.6280559614632182 1105 72-73 0.6170364279986842 1105 74-75 0.4023858428168019 1105 76-77 0.8085530854635223 1105 78-79 0.7035506112313321 1105 80-81 0.6182310917805474 1105 82-83 0.6572754889389358 1105 84-85 0.6287733748045206 1105 86-87 0.6160987329165692 1105 88-89 0.8470615245943662 1105 90-91 0.8121062891452908 1105 92-93 0.8421577387357999 1105 94-95 0.8342807663453407 1105 96-97 0.9524920494903775 1105 98-99 0.9516429980179666 1105 100-101 0.9801393124945434 1106 1 0.3480073895887905 1106 2 0.4157022778472701 1106 3 0.2391201959189715 1106 4 0.5137054786544724 1106 5 0.4552161313289602 1106 6 0.03172298807012908 1106 7 0.4767014155264562 1106 8 0.3206734567209395 1106 9 0.386924342458272 1106 10-11 0.16126382260432592 1106 12-13 0.34454363575515856 1106 14-15 0.5503428939126422 1106 16-17 0.31775622506241774 1106 18-19 0.03538196786607983 1106 20-21 0.27530249505969806 1106 22-23 0.3742169697193276 1106 24-25 0.5068398690698928 1106 26-27 0.19835524925713344 1106 28-29 0.28254816138207417 1106 30-31 0.3524358295316645 1106 32-33 0.39360215110168184 1106 34-35 0.29958103602456987 1106 36-37 0.21796791903279455 1106 38-39 0.2406223966376686 1106 40-41 0.4322484196755809 1106 42-43 0.05127458659063677 1106 44-45 0.2494095450109981 1106 46-47 0.003427336736343989 1106 48-49 0.4886178828340064 1106 50-51 0.00861245494920837 1106 52-53 0.26033606506501883 1106 54-55 -0.01604049172858879 1106 56-57 -0.3727184701923747 1106 58-59 -0.7424808482590919 1106 60-61 0.09985850040712307 1106 62-63 0.2308971812584808 1106 64-65 0.5140799567617513 1106 66-67 -0.049150150111227475 1106 68-69 -0.12661302362250737 1106 70-71 -0.10355785765559844 1106 72-73 0.4210118075438345 1106 74-75 0.4315740665172214 1106 76-77 0.39719358885812284 1106 78-79 0.5411700079170991 1106 80-81 0.6355234677594446 1106 82-83 0.6250829114244887 1106 84-85 0.5647732172917657 1106 86-87 0.6087460174371024 1106 88-89 0.7053781979250324 1106 90-91 0.6138356983916893 1106 92-93 0.7738574830804872 1106 94-95 0.5033406171524923 1106 96-97 0.4265917025584436 1106 98-99 0.4992934327935501 1106 100-101 -0.09167741535977036 1107 1 0.5297835275483678 1107 2 0.45054328025328516 1107 3 0.40322128960504244 1107 4 0.5054803211892747 1107 5 0.5668781929185087 1107 6 0.48129857847576574 1107 7 0.435525864931428 1107 8 0.3490282514514007 1107 9 0.4544550732516299 1107 10-11 0.50959406745541 1107 12-13 0.42995853503587966 1107 14-15 0.5687645846947476 1107 16-17 0.6175325273197743 1107 18-19 0.5830692001910336 1107 20-21 0.5927998528670884 1107 22-23 0.6872579477966383 1107 24-25 0.5928969514889246 1107 26-27 0.6378377823497061 1107 28-29 0.7764630219580297 1107 30-31 0.7542675127781848 1107 32-33 0.8258425487654932 1107 34-35 0.8074146039561683 1107 36-37 0.9213349966018782 1107 38-39 0.9954423246941957 1107 40-41 1.0857461850678405 1107 42-43 0.9983710973165998 1107 44-45 1.1097293806500304 1107 46-47 0.9516676127566086 1107 48-49 1.044253020125339 1107 50-51 1.1174012801727073 1107 52-53 1.0393600967744874 1107 54-55 0.9719843330336104 1107 56-57 1.0533084304090536 1107 58-59 1.1479275975945242 1107 60-61 1.0280358851340665 1107 62-63 1.1311504604999278 1107 64-65 1.0862829055908954 1107 66-67 0.9023764763972686 1107 68-69 1.148094636919332 1107 70-71 0.7856979366056684 1107 72-73 0.6004913311434734 1107 74-75 0.8834267214271172 1107 76-77 0.767856877941643 1107 78-79 0.9550461795261285 1107 80-81 1.0025036825155986 1107 82-83 0.9080688497464706 1107 84-85 0.9436061738460637 1107 86-87 0.6994919056482658 1107 88-89 0.8486893036648695 1107 90-91 0.826633705333272 1107 92-93 0.8533323858051425 1107 94-95 0.9599071569623199 1107 96-97 0.8065370261539719 1107 98-99 0.8250670936481832 1107 100-101 0.5100920243058216 1108 1 0.4338816096407214 1108 2 0.4032933525285074 1108 3 0.40850699202795226 1108 4 0.3997143729690862 1108 5 0.4112507978406086 1108 6 0.36966334384270993 1108 7 0.37907468607639316 1108 8 0.26830067061079177 1108 9 0.33406953875328327 1108 10-11 0.33296745202726186 1108 12-13 0.42367808717553146 1108 14-15 0.4861255548435395 1108 16-17 0.4437517488387215 1108 18-19 0.4671316675134065 1108 20-21 0.4659816312066596 1108 22-23 0.4100098038858988 1108 24-25 0.44203030235284047 1108 26-27 0.4213235087618372 1108 28-29 0.2589668433182837 1108 30-31 0.4992259314578007 1108 32-33 0.41594572549225717 1108 34-35 0.5769579398729618 1108 36-37 0.5716222906174906 1108 38-39 0.40526168276718266 1108 40-41 0.48023339235317763 1108 42-43 0.4215609873967452 1108 44-45 0.3878236829019457 1108 46-47 0.42214318373088133 1108 48-49 0.5984973057693281 1108 50-51 0.4610101897087304 1108 52-53 0.5404003986696324 1108 54-55 0.5551358249787697 1108 56-57 0.4248922727444935 1108 58-59 0.4428306949723222 1108 60-61 0.5917090119755457 1108 62-63 0.623724565671143 1108 64-65 0.6581402404496934 1108 66-67 0.4227559751455239 1108 68-69 0.28595770458739267 1108 70-71 0.410918414483298 1108 72-73 0.4646440886739356 1108 74-75 0.6191479435857765 1108 76-77 0.4738174310470171 1108 78-79 0.5810793692316771 1108 80-81 0.7256886132851612 1108 82-83 0.8228542461530068 1108 84-85 0.7208633521598031 1108 86-87 0.6040941843169243 1108 88-89 0.6498032647777237 1108 90-91 0.5709106993605708 1108 92-93 0.47365256504769704 1108 94-95 0.6428495889774766 1108 96-97 0.8038855249821886 1108 98-99 0.48321124273813254 1108 100-101 0.6639069643635178 1109 1 0.6210288222296363 1109 2 0.530526587966591 1109 3 0.6597768965280082 1109 4 0.566773531903074 1109 5 0.5657515060190264 1109 6 0.5161560381491626 1109 7 0.5779628199221136 1109 8 0.5681478394592716 1109 9 0.638007609591952 1109 10-11 0.5856878855782597 1109 12-13 0.5046294545847445 1109 14-15 0.587471874919359 1109 16-17 0.6983841990319064 1109 18-19 0.6448069937441261 1109 20-21 0.442439580509415 1109 22-23 0.685054455576676 1109 24-25 0.5325062789621242 1109 26-27 0.5579407591549739 1109 28-29 0.5354158102109423 1109 30-31 0.3995459135450474 1109 32-33 0.280000528608241 1109 34-35 0.5462800803422354 1109 36-37 0.6870927996728042 1109 38-39 0.5653718409617454 1109 40-41 0.4485245083590499 1109 42-43 0.23667174441865058 1109 44-45 0.5129955203125078 1109 46-47 0.49684949797464384 1109 48-49 0.6837895204931925 1109 50-51 0.7543154953189628 1109 52-53 0.7492596586231883 1109 54-55 0.7004484388861414 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-1.2630784385171445 2316 72-73 -0.8283744465646059 2316 74-75 -0.7843443426668699 2316 76-77 -0.9595574479145412 2316 78-79 -0.8609440337151177 2316 80-81 -0.7910874786778876 2316 82-83 -0.7446958375925092 2316 84-85 -0.8530919552124168 2316 86-87 -0.7482201034536118 2316 88-89 -0.9032737287581654 2316 90-91 -0.6976073648094356 2316 92-93 -0.7407539540174888 2316 94-95 -0.8824719807275834 2316 96-97 -0.8900818535213837 2316 98-99 -0.8935887952090553 2316 100-101 -0.5514310600273635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7136.0 3 2446.0 4 2542.0 5 2999.0 6 3687.0 7 4369.0 8 5607.0 9 7161.0 10 9018.0 11 11297.0 12 13587.0 13 15860.0 14 18266.0 15 21167.0 16 24027.0 17 26846.0 18 25765.0 19 24896.0 20 26221.0 21 28467.0 22 31338.0 23 35302.0 24 39514.0 25 44329.0 26 49836.0 27 56862.0 28 64686.0 29 74594.0 30 86164.0 31 100655.0 32 118572.0 33 142024.0 34 174116.0 35 214517.0 36 252688.0 37 246006.0 38 114298.0 39 6286.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.560012079013276 18.268474385869997 14.557840956214516 23.613672578902207 2 15.580062248020276 19.53238003935996 40.31337882179019 24.57417889082958 3 19.45617360121305 23.32700638932233 29.478118576029562 27.738701433435057 4 13.132245530496188 16.192464929709324 36.382061196883306 34.29322834291118 5 14.215715706324932 37.01887535883821 33.93948064619022 14.82592828864664 6 33.20065673742231 35.42427582971737 17.032062859153278 14.343004573707049 7 29.34020898001601 32.267345477328604 20.079752124058025 18.312693418597355 8 26.856265241042955 34.65414556368411 19.410054398799474 19.079534796473457 9 26.57975365893351 16.76691302649271 19.720792197151642 36.93254111742213 10-11 25.35888073437092 26.10448877331813 27.182707971669895 21.35392252064105 12-13 26.363967421749894 23.424088236004486 27.21125236044115 23.000691981804465 14-15 22.9233634551548 24.970262331606715 25.499807376348627 26.606566836889854 16-17 22.631004978596 27.99533489634002 26.497582451181835 22.87607767388215 18-19 22.749368392271663 26.500698555568114 28.539594429752135 22.210338622408088 20-21 23.232046550535788 25.80279013352651 28.74725879828719 22.217904517650513 22-23 22.912101067790246 25.719620461105848 28.71584932040438 22.65242915069953 24-25 22.642602126562135 25.947449788462635 28.85901175916476 22.55093632581047 26-27 22.260060815878358 26.27350541203156 28.555109288938276 22.91132448315181 28-29 22.409931881412497 26.69100500711953 28.411768513116254 22.48729459835172 30-31 22.59951373828876 26.20484859258731 28.217485205650426 22.9781524634735 32-33 22.157819062402456 26.214772551652622 28.268893563042802 23.358514822902123 34-35 22.99684342842134 26.45150699323659 27.988189825802273 22.563459752539796 36-37 23.16971988899306 25.847267436761268 28.092381335188136 22.890631339057528 38-39 22.541833622110566 26.260870176853583 28.092616603088782 23.104679597947065 40-41 23.091318663901962 26.13073349970318 28.13195454558243 22.645993290812427 42-43 22.89988014989703 26.131706635456876 27.851316475544202 23.117096739101896 44-45 22.75184154238331 26.47341557033608 28.11115473644115 22.663588150839463 46-47 23.21272973161145 26.670633103856655 27.36457207370353 22.752065090828363 48-49 22.842153973390396 26.744678875941553 27.362120554321457 23.051046596346595 50-51 23.026909387893646 26.901019220053684 27.343480604016634 22.728590788036033 52-53 23.424683577092605 26.456150171746145 27.37656793226453 22.742598318896718 54-55 22.788696478356687 26.809657507898244 27.49482456014915 22.906821453595917 56-57 22.893719226950044 26.988955223600698 27.253353799472468 22.86397174997679 58-59 22.793568306908906 26.431186739784422 27.453969501241616 23.321275452065056 60-61 22.4039307938361 26.686545681295794 27.766435643448308 23.143087881419802 62-63 22.65226640100794 26.64361686542237 27.89283014445193 22.81128658911776 64-65 22.417398597608674 26.744356948981938 27.588074915160554 23.250169538248837 66-67 22.32073247056452 26.814404691862144 27.528826026520647 23.336036811052693 68-69 22.94682111683742 26.369838849382955 27.676484480945923 23.006855552833702 70-71 22.73757472840581 26.41018324944277 27.339219779057828 23.51302224309359 72-73 22.71529164082913 26.57065457567634 27.3561313207211 23.357922462773434 74-75 22.776623269448805 26.917286146869092 27.214796066210845 23.091294517471265 76-77 22.8539144887599 26.56533251440835 27.313031339118165 23.267721657713583 78-79 22.936791419231344 26.829763404403746 27.1391796437869 23.094265532578014 80-81 23.165135034022217 26.735569038798545 26.870518673541433 23.2287772536378 82-83 23.117033929652216 26.838991088268287 26.963321208177472 23.08065377390202 84-85 22.99840743652601 26.924238140981018 26.99390400269287 23.083450419800105 86-87 23.18110630481365 26.822984244236487 26.835877092245514 23.16003235870435 88-89 23.077465961073585 26.862908766064002 26.592168291922334 23.467456980940078 90-91 22.973793350546032 26.887052044455377 26.74753398906038 23.391620615938212 92-93 23.248389499515742 26.43994345986872 26.9313990212621 23.380268019353437 94-95 22.962910510037055 26.27824632395957 27.146812066177283 23.61203109982609 96-97 23.387821589786505 25.81668488204711 27.152695692627237 23.64279783553915 98-99 23.05756126014985 26.147448242036848 26.73978169118761 24.055208806625693 100-101 23.75612681105425 25.498681642859573 27.020325112620675 23.724866433465504 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 218.0 1 155.5 2 128.0 3 186.0 4 183.0 5 181.5 6 318.0 7 688.5 8 1110.0 9 1292.0 10 1179.0 11 986.0 12 1004.0 13 1168.0 14 1336.5 15 1495.5 16 1644.0 17 1816.0 18 2044.0 19 2365.5 20 2772.5 21 3285.5 22 3961.5 23 4893.5 24 6168.5 25 7934.5 26 10767.5 27 14199.0 28 16927.5 29 19636.0 30 22897.5 31 27961.5 32 34848.0 33 37788.5 34 38317.5 35 41140.0 36 45436.0 37 49425.0 38 52575.0 39 55447.5 40 58711.0 41 62980.5 42 65870.0 43 67840.0 44 69809.5 45 73701.0 46 76444.5 47 77586.5 48 79007.0 49 78462.5 50 76994.0 51 75852.0 52 75397.5 53 76141.5 54 77327.0 55 73998.0 56 68257.5 57 62916.0 58 58926.0 59 53056.5 60 43936.0 61 36849.0 62 31321.0 63 24227.5 64 17146.5 65 12728.5 66 9620.5 67 7364.5 68 5769.0 69 4622.5 70 3730.0 71 2722.5 72 1998.0 73 1635.0 74 1268.0 75 945.5 76 715.0 77 509.5 78 353.0 79 229.5 80 145.0 81 93.0 82 53.0 83 31.0 84 24.0 85 19.5 86 12.5 87 10.0 88 6.5 89 1.5 90 1.5 91 4.0 92 3.0 93 1.5 94 1.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1799216276766155 2 0.048519771924256654 3 0.046879006690103045 4 0.0537702206735482 5 0.05958321750312097 6 0.06567748837283437 7 0.03722193131194182 8 0.03520613402426739 9 0.04237862204785316 10-11 0.04366779473183099 12-13 0.040761296317044594 14-15 0.035370210547682746 16-17 0.03387008233359945 18-19 0.04190983198095212 20-21 0.05168410487583861 22-23 0.048636969440981906 24-25 0.070060675498359 26-27 0.05353582564009767 28-29 0.0462695796031317 30-31 0.05491875633745572 32-33 0.05135595182900789 34-35 0.05114499629890242 36-37 0.04837913490418634 38-39 0.027939887987301418 40-41 0.025924090699626985 42-43 0.022830076258080183 44-45 0.004148792092074119 46-47 0.011930707202631225 48-49 0.01111032458555442 50-51 0.009211724814605247 52-53 0.009680514881506279 54-55 0.011274401108969783 56-57 0.009235164317950299 58-59 0.011461917135730195 60-61 0.011649433162490607 62-63 0.004148792092074119 64-65 0.007102169513550612 66-67 0.004500384642249893 68-69 0.009774272894886485 70-71 0.02397861192198771 72-73 0.02032204940015967 74-75 0.021048674003856266 76-77 0.012821408329743184 78-79 0.004781658682390511 80-81 0.006234907889783705 82-83 0.005625480802812366 84-85 0.005250448749291541 86-87 0.008696055741014116 88-89 0.031221418455608628 90-91 0.037995434922328514 92-93 0.023744216888537194 94-95 0.03410447736704997 96-97 0.03729224982197697 98-99 0.022689439238009874 100-101 0.12462783928563895 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2133151.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.10217137497047 #Duplication Level Percentage of deduplicated Percentage of total 1 76.34827004026012 41.30607189900675 2 12.962274111698388 14.025743508008985 3 4.497553771792275 7.299822747889515 4 2.082616539545592 4.506963077233742 5 1.0950911600492303 2.962340480609933 6 0.6708588459581701 2.1776952151466302 7 0.43835026805374006 1.660099092715538 8 0.3091625994914497 1.3381094352334213 9 0.23241435380114092 1.1316709079417109 >10 1.227082276377794 12.060334127691078 >50 0.087801032432366 3.232521027062194 >100 0.042353642805800895 4.15696386651169 >500 0.0032160596641370044 1.2068725665143203 >1k 0.002868377538284328 2.6860250644546633 >5k 8.692053146316146E-5 0.24876698397992172 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5290 0.2479899453906451 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4070 0.19079755722871938 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2969 0.13918377086291595 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2801 0.1313080977389786 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2767 0.12971421151151513 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2575 0.12071344222701534 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2569 0.12043216818687472 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2332 0.1093218436013203 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2186 0.10247750862456524 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.375801338020609E-5 2 0.0 0.0 0.0 0.0 9.375801338020609E-5 3 0.0 0.0 0.0 0.0 9.375801338020609E-5 4 0.0 0.0 0.0 0.0 9.375801338020609E-5 5 0.0 0.0 0.0 0.0 9.375801338020609E-5 6 0.0 0.0 0.0 0.0 1.4063702007030915E-4 7 0.0 0.0 0.0 0.0 1.4063702007030915E-4 8 0.0 0.0 0.0 0.0 1.8751602676041218E-4 9 0.0 0.0 0.0 0.0 1.8751602676041218E-4 10-11 0.0 0.0 0.0 4.6879006690103044E-5 2.3439503345051523E-4 12-13 0.0 0.0 0.0 4.6879006690103044E-5 3.7503205352082435E-4 14-15 0.0 0.0 0.0 4.6879006690103044E-5 3.7503205352082435E-4 16-17 0.0 0.0 0.0 9.375801338020609E-5 3.7503205352082435E-4 18-19 0.0 0.0 0.0 9.375801338020609E-5 4.4535056355597897E-4 20-21 0.0 0.0 0.0 1.4063702007030912E-4 5.156690735911335E-4 22-23 0.0 0.0 0.0 2.3439503345051523E-4 6.094270869713396E-4 24-25 0.0 0.0 0.0 5.156690735911335E-4 6.563060936614426E-4 26-27 0.0 0.0 0.0 0.0011016566572174214 7.735036103867002E-4 28-29 0.0 0.0 0.0 0.002953377421476492 7.969431137317517E-4 30-31 0.0 0.0 0.0 0.011368159122349989 7.969431137317517E-4 32-33 0.0 0.0 0.0 0.029650971731490172 7.969431137317517E-4 34-35 0.0 0.0 0.0 0.05634856604150386 8.438221204218548E-4 36-37 0.0 0.0 0.0 0.10137585196734783 8.438221204218548E-4 38-39 0.0 0.0 0.0 0.1700067177616587 8.438221204218548E-4 40-41 0.0 0.0 0.0 0.2431614077015645 8.438221204218548E-4 42-43 0.0 0.0 0.0 0.32166030440414206 8.907011271119579E-4 44-45 0.0 0.0 0.0 0.42123131461392094 9.610196371471124E-4 46-47 0.0 0.0 0.0 0.5377256462388269 9.84459140492164E-4 48-49 0.0 0.0 0.0 0.6594001081029894 0.001031338147182267 50-51 0.0 0.0 0.0 0.7990761085361515 0.001031338147182267 52-53 0.0 0.0 0.0 0.9329391121397408 0.001125096160562473 54-55 0.0 0.0 0.0 1.0783812303957854 0.0011719751672525762 56-57 0.0 0.0 0.0 1.2461377558363191 0.0011719751672525762 58-59 0.0 0.0 0.0 1.4269032056333564 0.0011719751672525762 60-61 0.0 0.0 0.0 1.6257639520127736 0.0012422936772877307 62-63 0.0 0.0 0.0 1.8247887749156062 0.0014532492073931944 64-65 0.0 0.0 0.0 2.035533349490964 0.0014532492073931944 66-67 0.0 0.0 0.0 2.2791869867627748 0.0015001282140832974 68-69 0.0 0.0 0.0 2.548061529633861 0.0015470072207734004 70-71 0.0 0.0 0.0 2.8272025749700793 0.0015938862274635034 72-73 0.0 0.0 0.0 3.119540060689562 0.001617325730808555 74-75 0.0 0.0 0.0 3.414784044823831 0.0016642047374986581 76-77 0.0 0.0 0.0 3.7392805291327242 0.0017814022542239156 78-79 0.0 0.0 0.0 4.1027803470077835 0.0017814022542239156 80-81 4.6879006690103044E-5 0.0 0.0 4.482242466660822 0.0018282812609140189 82-83 9.375801338020609E-5 0.0 0.0 4.877573130078462 0.0019220392742942249 84-85 1.1719751672525762E-4 0.0 0.0 5.282303034337466 0.002062676294364534 86-87 1.4063702007030915E-4 0.0 0.0 5.705128235178851 0.0020861157977095854 88-89 1.4063702007030915E-4 2.3439503345051522E-5 0.0 6.155635489470741 0.002109555301054637 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2970 0.0 58.34745 1 GTATCAA 5865 0.0 46.59302 1 ATCAACG 7755 0.0 34.870068 3 TATCAAC 7775 0.0 34.71843 2 TCAACGC 7895 0.0 34.191532 4 CAACGCA 7935 0.0 34.136555 5 AACGCAG 8225 0.0 32.986088 6 ACGCAGA 9360 0.0 28.777718 7 CGCAGAG 9465 0.0 28.207518 8 TGGTATC 1250 0.0 24.712431 2 GCAGAGT 10610 0.0 24.491245 9 GTACATG 7400 0.0 23.932508 1 TACATGG 7255 0.0 23.581814 2 AGGTAAG 1235 0.0 22.318918 2 ACATGGG 7280 0.0 22.261034 3 AGAGTAC 9410 0.0 21.908806 10-11 GGTAAGG 1345 0.0 21.907444 3 TAAGGTG 1395 0.0 21.80206 5 CAGAGTA 9765 0.0 21.4042 10-11 GTAAGGT 1340 0.0 21.27986 4 >>END_MODULE