FastQCFastQC Report
Thu 26 May 2016
SRR938666_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938666_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133151
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53420.2504276537385305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45180.21179935222588553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30140.14129332616397056No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29120.13651166748158006No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27780.13022988058510626No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27770.13018300157841617No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27370.12830784131081205No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25010.11724439573194771No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG23550.11040006075519267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.059.6503831
GTATCAA58600.046.9931031
TATCAAC78250.034.9491542
ATCAACG78500.034.702013
TCAACGC79400.034.3086624
CAACGCA81000.033.6896555
AACGCAG85000.032.017536
ACGCAGA96900.028.0365377
CGCAGAG97250.027.9356378
TAAGGTG13900.026.677775
TGGTATC13050.026.6051392
GTACCGT2409.094947E-1225.726716
GCAGAGT108800.024.2715789
AGGTAAG13050.024.053962
GTAAGGT14300.023.6043474
GGTAAGG13800.023.4260753
GTACATG71150.022.3935571
TACATGG70150.022.3737832
AGAGTAC99700.022.10420810-11
AAGGTAA13150.021.7009451
Produced by FastQC (version 0.11.5)