##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938666_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2133151 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75391849897171 31.0 31.0 34.0 30.0 34.0 2 31.986904818271185 31.0 31.0 34.0 30.0 34.0 3 32.0506054189319 33.0 31.0 34.0 30.0 34.0 4 35.73096044302537 37.0 35.0 37.0 33.0 37.0 5 35.59103879659715 37.0 35.0 37.0 33.0 37.0 6 35.61785171326362 37.0 35.0 37.0 33.0 37.0 7 35.55687290773133 37.0 35.0 37.0 33.0 37.0 8 35.54267935087577 37.0 35.0 37.0 33.0 37.0 9 37.124035757431145 39.0 37.0 39.0 33.0 39.0 10-11 37.03125774968579 39.0 37.0 39.0 33.0 39.0 12-13 36.92045429507803 39.0 37.0 39.0 33.0 39.0 14-15 37.968050550570496 40.0 37.0 41.0 33.0 41.0 16-17 37.88810520211649 40.0 37.0 41.0 33.0 41.0 18-19 37.995380073890686 40.0 37.0 41.0 33.0 41.0 20-21 38.0461950419825 40.0 37.0 41.0 33.0 41.0 22-23 37.94598507091153 40.0 37.0 41.0 33.0 41.0 24-25 37.84083428693046 40.0 37.0 41.0 32.0 41.0 26-27 37.64519201875535 40.0 37.0 41.0 32.0 41.0 28-29 37.51758665935979 39.5 36.5 41.0 32.0 41.0 30-31 37.273862469182916 39.0 36.0 41.0 31.0 41.0 32-33 37.06013967131253 39.0 36.0 41.0 31.0 41.0 34-35 36.896961349665354 39.0 35.5 40.5 30.0 41.0 36-37 36.88570640334416 39.0 36.0 41.0 30.0 41.0 38-39 36.75134273194912 39.0 35.0 41.0 30.0 41.0 40-41 36.5717462101839 39.0 35.0 40.5 30.0 41.0 42-43 36.34578283487667 39.0 35.0 40.0 30.0 41.0 44-45 36.06223281896125 38.5 35.0 40.0 29.5 41.0 46-47 35.70728560706673 38.0 35.0 40.0 28.5 41.0 48-49 35.337160613571186 38.0 34.0 40.0 27.0 41.0 50-51 34.966999992030566 38.0 34.0 40.0 26.0 41.0 52-53 34.4652014789389 38.0 33.0 40.0 24.5 41.0 54-55 34.13425256814918 37.0 33.0 40.0 24.0 41.0 56-57 33.783610724229085 37.0 33.0 40.0 23.0 41.0 58-59 33.322695158476826 36.5 32.0 39.0 22.5 41.0 60-61 32.949113072632926 36.0 31.5 39.0 22.0 40.0 62-63 32.47767199790357 35.5 31.0 39.0 20.5 40.0 64-65 31.96848418138238 35.0 31.0 38.5 18.5 40.0 66-67 31.463712601686424 35.0 30.0 38.0 17.5 40.0 68-69 30.92286621997224 34.0 29.0 37.5 13.5 39.5 70-71 30.33605731614874 34.0 29.0 37.0 9.5 39.0 72-73 29.885802505307876 34.0 29.0 36.0 8.0 39.0 74-75 29.370643475309528 34.0 28.0 36.0 7.0 38.5 76-77 28.12569621184811 31.5 26.5 34.5 6.0 36.5 78-79 28.82747095728338 33.0 28.0 35.0 2.0 37.0 80-81 28.55719004421159 33.0 27.0 35.0 2.0 37.0 82-83 28.081206159338933 33.0 26.5 35.0 2.0 36.0 84-85 27.550819187202407 33.0 26.0 35.0 2.0 36.0 86-87 26.981746486770042 32.0 25.5 35.0 2.0 35.0 88-89 26.319161184557494 32.0 24.5 34.0 2.0 35.0 90-91 25.76539354222931 31.5 23.5 34.0 2.0 35.0 92-93 25.01154606495274 31.0 19.5 34.0 2.0 35.0 94-95 24.224800307151252 31.0 15.5 34.0 2.0 35.0 96-97 23.192469731397356 30.5 2.0 34.0 2.0 35.0 98-99 21.919808302365844 30.0 2.0 34.0 2.0 35.0 100-101 18.26825667756291 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3807654325487313 1101 2 0.3975371407795407 1101 3 0.38112534253468766 1101 4 0.2489112029692322 1101 5 0.278514295711517 1101 6 0.23281893336253034 1101 7 0.3622225566196349 1101 8 0.36265462784678704 1101 9 0.3588276103774035 1101 10-11 0.448534363448708 1101 12-13 0.4520823253024844 1101 14-15 0.5916393667573772 1101 16-17 0.5448324568531575 1101 18-19 0.5802304185851526 1101 20-21 0.5375629162527176 1101 22-23 0.5586179762354675 1101 24-25 0.5147832036483209 1101 26-27 0.49411585068932595 1101 28-29 0.544595845618872 1101 30-31 0.5510504855951908 1101 32-33 0.4415283326854009 1101 34-35 0.36673999397665824 1101 36-37 0.4978741856179667 1101 38-39 0.5180238539981801 1101 40-41 0.6576143732488333 1101 42-43 0.6588184928493561 1101 44-45 0.6389743280130844 1101 46-47 0.7345502599636049 1101 48-49 0.7631577317735321 1101 50-51 0.7339277079533773 1101 52-53 0.816194713665304 1101 54-55 0.6929029706717031 1101 56-57 0.6304575455700885 1101 58-59 0.7776311354341985 1101 60-61 0.5861633900073073 1101 62-63 0.604069801114484 1101 64-65 0.6113498673506648 1101 66-67 0.4855964420575525 1101 68-69 0.4760361702798974 1101 70-71 0.40688946981629925 1101 72-73 0.3641839398063027 1101 74-75 0.6116355228997818 1101 76-77 0.6502886971011392 1101 78-79 0.5261066231444111 1101 80-81 0.74055213703841 1101 82-83 0.7837416429815498 1101 84-85 0.8343950989337579 1101 86-87 0.7096852380559007 1101 88-89 0.41771631325402225 1101 90-91 0.594819532324486 1101 92-93 0.7281037983074476 1101 94-95 0.7845902338751678 1101 96-97 0.774841065447454 1101 98-99 0.5750709595613621 1101 100-101 0.2707401065325463 1102 1 0.253938890120164 1102 2 0.30427867346834603 1102 3 0.36271180201816833 1102 4 0.23929082935726598 1102 5 0.2601595528502685 1102 6 0.2336187610090974 1102 7 0.29465821582662954 1102 8 0.28555519248381245 1102 9 0.3370774151332796 1102 10-11 0.26549097885818185 1102 12-13 0.3417812902842172 1102 14-15 0.4087167193368444 1102 16-17 0.41064857728511583 1102 18-19 0.44717154911595713 1102 20-21 0.3994081751868137 1102 22-23 0.36907902166676365 1102 24-25 0.47641952892951167 1102 26-27 0.4426115732721172 1102 28-29 0.47471213930193557 1102 30-31 0.5070696150308152 1102 32-33 0.4268760467582382 1102 34-35 0.44191750903513594 1102 36-37 0.5737835295079492 1102 38-39 0.5594750788745273 1102 40-41 0.5083354299908152 1102 42-43 0.51668032284784 1102 44-45 0.6716965696849186 1102 46-47 0.3147370210655893 1102 48-49 0.6050205402368007 1102 50-51 0.6656342313512553 1102 52-53 0.3543474349644171 1102 54-55 0.5644155066908709 1102 56-57 0.5512205236937646 1102 58-59 0.3946113147894863 1102 60-61 0.6263946757459564 1102 62-63 0.5446944925390085 1102 64-65 0.6011381509079676 1102 66-67 0.695805241059336 1102 68-69 0.5345028082209922 1102 70-71 0.4325447719285265 1102 72-73 0.29859335999858416 1102 74-75 0.3648406683678722 1102 76-77 0.38363123159426493 1102 78-79 0.3533900817032851 1102 80-81 0.46178661849695146 1102 82-83 0.36191312566990774 1102 84-85 0.28004878523255883 1102 86-87 0.4556172033338619 1102 88-89 0.45866394294549195 1102 90-91 0.0898026560509102 1102 92-93 0.1508439036943905 1102 94-95 0.2264855830255179 1102 96-97 0.23082473675509974 1102 98-99 0.18380375788091285 1102 100-101 -0.11006919157605566 1103 1 0.33394256205868444 1103 2 0.33721333919079655 1103 3 0.2964398687425387 1103 4 0.2394836061296317 1103 5 0.19482134108432803 1103 6 0.17248623025661658 1103 7 0.3149928867947125 1103 8 0.26375699173368616 1103 9 0.40305034770074855 1103 10-11 0.5106433643068087 1103 12-13 0.4418193210760464 1103 14-15 0.5643433094173673 1103 16-17 0.542254132403535 1103 18-19 0.4830254059628061 1103 20-21 0.30603667992099304 1103 22-23 0.5046974810262697 1103 24-25 0.6139572318696906 1103 26-27 0.6160110014988334 1103 28-29 0.5734754828642394 1103 30-31 0.5785419312371829 1103 32-33 0.6633292164280249 1103 34-35 0.6417470261752243 1103 36-37 0.5628975431990355 1103 38-39 0.5946961820297716 1103 40-41 0.5781402926865411 1103 42-43 0.6272194421071546 1103 44-45 0.732085532886849 1103 46-47 0.5310522307595065 1103 48-49 0.7238726660269208 1103 50-51 0.7715263812712578 1103 52-53 0.9416340035378639 1103 54-55 0.9666829287277494 1103 56-57 0.9648519397506305 1103 58-59 0.7909531460376868 1103 60-61 0.7543833714209356 1103 62-63 0.6219409320700464 1103 64-65 0.7447168484189142 1103 66-67 0.7731185623277277 1103 68-69 0.5313128116306487 1103 70-71 0.5116278364235001 1103 72-73 0.45227513406945263 1103 74-75 0.5122322806111654 1103 76-77 0.4430346727252221 1103 78-79 0.5406779616833504 1103 80-81 0.5218156005831354 1103 82-83 0.3209401931024338 1103 84-85 0.20254137578518439 1103 86-87 0.45408351329793106 1103 88-89 0.416592051634872 1103 90-91 0.48209027374965174 1103 92-93 0.4254531045231964 1103 94-95 0.5582753907733071 1103 96-97 0.39279328922447476 1103 98-99 0.5302268963392258 1103 100-101 0.4007027023573819 1104 1 0.21033554944707689 1104 2 0.24491484324680357 1104 3 0.1873785816041007 1104 4 0.23176823464622487 1104 5 0.13838220358663733 1104 6 0.1100190684969462 1104 7 -0.04772733398162643 1104 8 0.11425492712353247 1104 9 0.24792939100329647 1104 10-11 0.2582650143756311 1104 12-13 0.26675338619610045 1104 14-15 0.3652679911986638 1104 16-17 0.3452266283854257 1104 18-19 0.31710809717395705 1104 20-21 0.2630379650266903 1104 22-23 0.3408096831565146 1104 24-25 0.28659507495931535 1104 26-27 0.410819587934256 1104 28-29 0.4047562646770686 1104 30-31 0.3575095658675096 1104 32-33 0.37008308423440894 1104 34-35 0.30979914358496785 1104 36-37 0.3610623054630935 1104 38-39 0.27779036684037095 1104 40-41 0.3141763962322912 1104 42-43 0.3961133812661828 1104 44-45 0.3774704358234686 1104 46-47 0.36833045958756117 1104 48-49 0.4141843730641668 1104 50-51 0.5055870224115253 1104 52-53 0.5106001222258172 1104 54-55 0.5998059250567636 1104 56-57 0.5965070129034657 1104 58-59 0.4841619395370458 1104 60-61 0.347111886813785 1104 62-63 0.41887506781924344 1104 64-65 0.620873615809618 1104 66-67 0.45570370057994936 1104 68-69 0.3676493904587872 1104 70-71 0.25742023500609434 1104 72-73 0.1714341047445771 1104 74-75 0.15238589036280814 1104 76-77 0.1700726677536224 1104 78-79 0.24419128733361362 1104 80-81 0.2268229046691168 1104 82-83 0.04501736318255212 1104 84-85 0.15283763054619115 1104 86-87 0.2376497935715598 1104 88-89 0.34994635837547605 1104 90-91 0.21135373247554057 1104 92-93 0.09021429191951569 1104 94-95 0.18888933020796372 1104 96-97 0.2553922776774762 1104 98-99 0.16172391527299723 1104 100-101 -0.038776743703497374 1105 1 0.17010015928727285 1105 2 0.26591843071098253 1105 3 0.24339635278172977 1105 4 0.09067689891578112 1105 5 0.05192329123028827 1105 6 0.09422273964811723 1105 7 0.11443794371353988 1105 8 0.1419036304880379 1105 9 0.17274463352121217 1105 10-11 0.14327899297391866 1105 12-13 0.25460985070810693 1105 14-15 0.4015656830377523 1105 16-17 0.2898575339073304 1105 18-19 0.2638199985710017 1105 20-21 0.27945876698594674 1105 22-23 0.2746113285903391 1105 24-25 0.21647553384295293 1105 26-27 0.2802254308447516 1105 28-29 0.21706143091265062 1105 30-31 0.4210094253636214 1105 32-33 0.3777796204546675 1105 34-35 0.4088730486289478 1105 36-37 0.3131556082479392 1105 38-39 0.3467839282754426 1105 40-41 0.41772458086055764 1105 42-43 0.4193103677142247 1105 44-45 0.48865731419633107 1105 46-47 0.2990098420268268 1105 48-49 0.16733381381767742 1105 50-51 0.06201369066440776 1105 52-53 0.10901468458185803 1105 54-55 0.16184844132751408 1105 56-57 0.46063257620097886 1105 58-59 0.3986586587200307 1105 60-61 0.1725258604893085 1105 62-63 0.38682725957730213 1105 64-65 0.24944759366948333 1105 66-67 0.33690222949400805 1105 68-69 0.22143273962017673 1105 70-71 0.1398077139561522 1105 72-73 0.11077755162282799 1105 74-75 0.08232398660694429 1105 76-77 0.09348153816391402 1105 78-79 0.24131311138172507 1105 80-81 0.24853906220576505 1105 82-83 0.27938707505728644 1105 84-85 0.2299197032759288 1105 86-87 0.2595640923546121 1105 88-89 0.022169663149941954 1105 90-91 -3.001931209851705E-4 1105 92-93 0.11434650094827603 1105 94-95 0.37050044827019946 1105 96-97 0.13317892098836026 1105 98-99 0.07069985731565609 1105 100-101 -0.3379269515877148 1106 1 0.13301095456511902 1106 2 0.06007410177589634 1106 3 0.1783048136398122 1106 4 0.1206743948668958 1106 5 0.016411637305999704 1106 6 -0.09112918019000915 1106 7 0.05368487120517074 1106 8 0.09186243484508338 1106 9 0.060029517547398825 1106 10-11 0.0722548773676337 1106 12-13 0.039443493008761266 1106 14-15 0.032069037251616805 1106 16-17 -0.09343823869271972 1106 18-19 0.18452050624672012 1106 20-21 0.13025491317393545 1106 22-23 0.2853499624297342 1106 24-25 0.15883313625653273 1106 26-27 0.004478860785908978 1106 28-29 0.21675529074245503 1106 30-31 0.31000645281527284 1106 32-33 0.306416953400273 1106 34-35 0.05622713729651707 1106 36-37 -0.3398039150134622 1106 38-39 -0.08697236718617063 1106 40-41 0.22203447306528545 1106 42-43 0.242290266663737 1106 44-45 0.1959634826380423 1106 46-47 -0.14669349280085697 1106 48-49 -0.025028332232992057 1106 50-51 -0.008467207885253458 1106 52-53 0.07131875285353573 1106 54-55 -0.21104371494129026 1106 56-57 0.004815408118709286 1106 58-59 -0.10390647684190668 1106 60-61 -0.3229652360013944 1106 62-63 -0.41983512597352757 1106 64-65 -0.5328968422935034 1106 66-67 -0.3588761258305304 1106 68-69 -0.9420262202302254 1106 70-71 -0.8046175462427954 1106 72-73 -0.5371393422256929 1106 74-75 -0.2351792141329625 1106 76-77 -0.30328672422810143 1106 78-79 -0.7003837452291641 1106 80-81 -1.291421519218229 1106 82-83 -0.7035253155420307 1106 84-85 -0.6005439698406825 1106 86-87 -0.33464357811255496 1106 88-89 -0.10540324707990578 1106 90-91 -0.19943962825602313 1106 92-93 -0.41336887754679097 1106 94-95 -0.5922713117126754 1106 96-97 -0.6141975829555157 1106 98-99 -0.9057855081341195 1106 100-101 -1.0803153497637972 1107 1 0.21383163775951175 1107 2 0.25380369291139715 1107 3 0.26605641435829597 1107 4 0.1794681174279802 1107 5 0.06108996911483899 1107 6 0.06808365968127106 1107 7 -1.4081833206347483E-4 1107 8 0.13163868970885062 1107 9 0.1956814242704965 1107 10-11 0.20914928602991978 1107 12-13 0.19297087939447977 1107 14-15 0.4086521806156469 1107 16-17 0.4265061686267515 1107 18-19 0.3155217195226072 1107 20-21 0.3940620481208654 1107 22-23 0.30418279260112513 1107 24-25 0.3120862322427769 1107 26-27 0.2647978275424663 1107 28-29 0.3211582342003254 1107 30-31 0.3216105757416017 1107 32-33 0.26856073133286884 1107 34-35 0.3997364667246188 1107 36-37 0.38277395660362146 1107 38-39 0.30068804036709906 1107 40-41 0.4071883916462653 1107 42-43 0.46478895149163435 1107 44-45 0.40483058627361146 1107 46-47 0.4348712697493653 1107 48-49 0.40825867386656967 1107 50-51 0.2988209783146729 1107 52-53 0.4700242364498237 1107 54-55 0.4005288912480012 1107 56-57 0.33949096740256124 1107 58-59 0.293503976920114 1107 60-61 0.16197505597471462 1107 62-63 0.4353628449489264 1107 64-65 0.5356240195747226 1107 66-67 0.49955744494468846 1107 68-69 0.5030396726571027 1107 70-71 0.4576289572951353 1107 72-73 0.592803809163648 1107 74-75 0.5885831812654381 1107 76-77 0.4612873969762674 1107 78-79 0.6024217972931503 1107 80-81 0.775336696065569 1107 82-83 0.6920486366252021 1107 84-85 0.7590523236646547 1107 86-87 0.7025380751970154 1107 88-89 0.4131258691718287 1107 90-91 0.5181911425059234 1107 92-93 0.5217473236358359 1107 94-95 0.4388415924559048 1107 96-97 0.5627339427863127 1107 98-99 0.36645709333160426 1107 100-101 -0.04968822988803012 1108 1 0.16614421426360337 1108 2 0.16385563880647425 1108 3 0.12934975027508244 1108 4 0.08144446036315856 1108 5 0.0183929960627367 1108 6 0.05312730161443113 1108 7 0.13320081896318214 1108 8 0.17794980840629648 1108 9 0.151779992298394 1108 10-11 0.11505271095287384 1108 12-13 0.15568724373542153 1108 14-15 0.22653649258626984 1108 16-17 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-0.35348943195609905 2316 74-75 0.14399434194686478 2316 76-77 0.04767762557933253 2316 78-79 -0.23024599057293216 2316 80-81 -0.2527786327893651 2316 82-83 -0.18857822959575898 2316 84-85 -0.010861034076899756 2316 86-87 0.04117516982902103 2316 88-89 0.18267798414224146 2316 90-91 0.2618527920452287 2316 92-93 0.1637001308482091 2316 94-95 0.09246578155989837 2316 96-97 0.37077761515712027 2316 98-99 0.5082820854106664 2316 100-101 0.3928583676291133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 34.0 5 89.0 6 158.0 7 419.0 8 1051.0 9 1790.0 10 2967.0 11 4526.0 12 6396.0 13 8708.0 14 11499.0 15 14930.0 16 18278.0 17 22123.0 18 21233.0 19 18786.0 20 18733.0 21 19734.0 22 21660.0 23 24351.0 24 28284.0 25 33059.0 26 39541.0 27 47439.0 28 57233.0 29 67275.0 30 80315.0 31 95514.0 32 115324.0 33 141865.0 34 179963.0 35 234521.0 36 304726.0 37 330447.0 38 153947.0 39 6226.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.95675195732858 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22.542098519982883 26.56459856803386 28.75319187436801 22.140111037615245 20-21 23.013115339701688 25.81734720139362 28.950810327070144 22.218727131834548 22-23 22.699025994878 25.765006790424117 28.90833794700891 22.627629267688974 24-25 22.585777669643257 26.02637747870457 28.962464116957797 22.425380734694375 26-27 22.147441134213782 26.384943223440217 28.64515218593447 22.82246345641153 28-29 22.341573568865964 26.709993807283215 28.555034313088946 22.39339831076187 30-31 22.456731848800203 26.302474602126154 28.34002374890479 22.900769800168856 32-33 22.1074129304489 26.242680429092925 28.37544083845916 23.274465801999014 34-35 22.88693352011856 26.470570309119235 28.12767970459429 22.514816466167918 36-37 23.07236859684907 25.88405685678778 28.230751813398875 22.812822732964268 38-39 22.419857336107757 26.29554177094783 28.271463166335803 23.013137726608615 40-41 22.944742766434043 26.189287412062214 28.26369569844382 22.60227412305993 42-43 22.79022495871124 26.144564814333005 28.006368056209908 23.05884217074585 44-45 22.659910775165653 26.43353106121673 28.267696067389526 22.63886209622809 46-47 23.137039993886976 26.745762489387765 27.417257381216803 22.699940135508456 48-49 22.761070360232353 26.789266207596178 27.439524909394603 23.01013852277687 50-51 22.92894408318961 26.92312921119977 27.43743879359689 22.71048791201373 52-53 23.379943567051747 26.501124392975463 27.392223991644286 22.726708048328508 54-55 22.756569975590104 26.821636161715695 27.52674330134154 22.895050561352665 56-57 22.86045385441537 26.97110987454709 27.2701744977266 22.89826177331094 58-59 22.714636704105803 26.543784289063453 27.393255329791465 23.348323677039275 60-61 22.38540544012121 26.773819574891792 27.652168083740907 23.188606901246093 62-63 22.56305343597336 26.700008578858224 27.82205760398584 22.914880381182577 64-65 22.32828337046932 26.76732683246521 27.580302566484978 23.32408723058049 66-67 22.22179510198303 26.916052277642116 27.451528233611434 23.410624386763416 68-69 22.843627512796914 26.41909943646218 27.551050544950122 23.18622250579078 70-71 22.59354067776309 26.449763600722783 27.2859684705643 23.670727250949827 72-73 22.578781342717885 26.617876559137166 27.261548760495625 23.541793337649327 74-75 22.62556659139461 27.03777182206042 26.98843166751908 23.348229919025894 76-77 22.61333117064849 26.589210046546164 27.356642825566496 23.440815957238843 78-79 22.661241515485777 26.85250598762113 27.192167830594272 23.294084666298822 80-81 22.86143703555425 26.777202694362558 26.905699888965483 23.45566038111771 82-83 22.84733502567817 26.8517698129748 27.017136749432176 23.283758411914857 84-85 22.64524844430036 26.998793039148055 26.99244061361149 23.363517902940096 86-87 22.824562591958063 26.849539321894767 26.819231397603165 23.506666688544005 88-89 22.60054379673253 26.942385673768836 26.604505074654856 23.852565454843774 90-91 22.500947571907055 26.91329242385495 26.892407165495396 23.693352838742598 92-93 22.71479214600878 26.531878946075537 26.9678575915602 23.78547131635548 94-95 22.368857150759606 26.4263289378014 27.139850859128117 24.06496305231088 96-97 22.702424722863032 25.94403771697362 27.19240222562772 24.161135334535626 98-99 22.325522742066255 26.287666997710662 26.811985370933744 24.57482488928934 100-101 22.385457566167975 26.009463467298723 27.216129382338472 24.388949584194833 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 203.0 1 145.0 2 117.5 3 177.5 4 173.0 5 173.0 6 301.5 7 646.5 8 1077.0 9 1262.5 10 1125.5 11 973.0 12 1024.5 13 1135.0 14 1292.5 15 1456.5 16 1608.5 17 1825.5 18 2093.5 19 2381.5 20 2744.5 21 3270.0 22 4009.5 23 5029.5 24 6394.5 25 8185.5 26 10956.0 27 14410.5 28 17200.5 29 19993.5 30 23382.5 31 28397.5 32 35660.5 33 38522.5 34 38403.5 35 41068.0 36 45307.5 37 49711.5 38 53216.0 39 55535.5 40 58585.0 41 63486.5 42 66731.5 43 68529.0 44 70342.0 45 74058.0 46 76471.0 47 77740.0 48 79348.5 49 78734.0 50 77375.0 51 76035.5 52 75517.0 53 76858.0 54 78533.5 55 74623.0 56 68336.5 57 62701.5 58 58350.0 59 52501.5 60 43020.5 61 36056.5 62 30918.0 63 23326.5 64 15850.5 65 11513.0 66 8535.5 67 6699.5 68 5326.0 69 4149.0 70 3285.5 71 2323.0 72 1688.0 73 1444.5 74 1145.5 75 839.5 76 632.5 77 423.5 78 251.5 79 145.5 80 89.5 81 56.0 82 33.5 83 20.5 84 12.5 85 7.0 86 4.5 87 2.5 88 0.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.563060936614426E-4 2 0.04983238411157954 3 9.84459140492164E-4 4 0.0 5 0.08681992039007085 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.5783453679556674E-4 26-27 0.008649176734324013 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01031338147182267 36-37 9.375801338020609E-5 38-39 0.004476945138904841 40-41 0.005109811729221232 42-43 1.8751602676041218E-4 44-45 2.3439503345051522E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 9.375801338020609E-5 68-69 9.375801338020609E-4 70-71 7.031851003515457E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001429809704048143 82-83 7.500641070416487E-4 84-85 0.005039493219186077 86-87 0.0021329948043996884 88-89 4.6879006690103044E-5 90-91 0.0031643329515819553 92-93 8.907011271119577E-4 94-95 0.0 96-97 0.0 98-99 2.3439503345051522E-5 100-101 2.3439503345051522E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2133151.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.55088422461055 #Duplication Level Percentage of deduplicated Percentage of total 1 74.19177607146705 38.24651658678428 2 14.014274068813865 14.448964400267705 3 4.989892718815368 7.717001455226345 4 2.2494103626725335 4.63836372719084 5 1.2303780155693798 3.171353731656158 6 0.7378943435951256 2.282346352600039 7 0.47199045359169256 1.7032067659758732 8 0.34804225640436437 1.4353508852138885 9 0.25067277586887643 1.1630162922370375 >10 1.3626544521285069 12.806092779084635 >50 0.09671094511173586 3.4181105828519174 >100 0.049746857143043846 4.642726887440302 >500 0.0034604693764644003 1.267509715026582 >1k 0.0030051444585085458 2.8086606940861163 >5k 9.106498359116806E-5 0.25077914435822146 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5342 0.2504276537385305 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4518 0.21179935222588553 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3014 0.14129332616397056 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2912 0.13651166748158006 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2778 0.13022988058510626 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2777 0.13018300157841617 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2737 0.12830784131081205 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2501 0.11724439573194771 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2355 0.11040006075519267 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.375801338020609E-5 2 0.0 0.0 0.0 0.0 9.375801338020609E-5 3 0.0 0.0 0.0 0.0 9.375801338020609E-5 4 0.0 0.0 0.0 0.0 9.375801338020609E-5 5 0.0 0.0 0.0 0.0 1.4063702007030915E-4 6 0.0 0.0 0.0 0.0 1.8751602676041218E-4 7 0.0 0.0 0.0 4.6879006690103044E-5 1.8751602676041218E-4 8 0.0 0.0 0.0 4.6879006690103044E-5 1.8751602676041218E-4 9 0.0 0.0 0.0 4.6879006690103044E-5 2.3439503345051523E-4 10-11 0.0 0.0 0.0 9.375801338020609E-5 2.3439503345051523E-4 12-13 0.0 0.0 0.0 9.375801338020609E-5 4.219110602109274E-4 14-15 0.0 0.0 0.0 9.375801338020609E-5 4.219110602109274E-4 16-17 0.0 0.0 0.0 1.6407652341536067E-4 4.219110602109274E-4 18-19 0.0 0.0 0.0 1.8751602676041218E-4 4.6879006690103047E-4 20-21 0.0 0.0 0.0 2.3439503345051523E-4 5.39108576936185E-4 22-23 0.0 0.0 0.0 3.2815304683072135E-4 6.328665903163912E-4 24-25 0.0 0.0 0.0 6.094270869713396E-4 7.031851003515457E-4 26-27 0.0 0.0 0.0 0.0011954146705976277 7.031851003515457E-4 28-29 4.6879006690103044E-5 0.0 0.0 0.0030236959315116463 7.031851003515457E-4 30-31 4.6879006690103044E-5 0.0 0.0 0.01139159862569504 7.031851003515457E-4 32-33 4.6879006690103044E-5 0.0 0.0 0.029908806268285744 7.500641070416487E-4 34-35 4.6879006690103044E-5 0.0 0.0 0.05698143263182025 7.500641070416487E-4 36-37 4.6879006690103044E-5 0.0 0.0 0.10210247657104443 7.500641070416487E-4 38-39 4.6879006690103044E-5 0.0 0.0 0.1714130879623618 7.500641070416487E-4 40-41 4.6879006690103044E-5 0.0 0.0 0.2455287975394147 7.500641070416487E-4 42-43 4.6879006690103044E-5 0.0 0.0 0.3247777583490339 7.735036103867002E-4 44-45 4.6879006690103044E-5 0.0 0.0 0.4248644376324039 8.907011271119578E-4 46-47 9.375801338020609E-5 0.0 0.0 0.5420853938610066 9.84459140492164E-4 48-49 9.375801338020609E-5 0.0 0.0 0.664252085295415 0.00107821715387237 50-51 9.375801338020609E-5 0.0 0.0 0.804326557285443 0.00107821715387237 52-53 9.375801338020609E-5 0.0 0.0 0.9385880324458982 0.0011016566572174214 54-55 9.375801338020609E-5 0.0 0.0 1.0842176667287031 0.0011719751672525762 56-57 9.375801338020609E-5 0.0 0.0 1.2533102438599049 0.0011719751672525762 58-59 9.375801338020609E-5 0.0 0.0 1.4355758218710255 0.0011719751672525762 60-61 9.375801338020609E-5 0.0 0.0 1.6351631928541392 0.0011954146705976277 62-63 9.375801338020609E-5 0.0 0.0 1.8351021563874288 0.0013126121873228852 64-65 9.375801338020609E-5 0.0 0.0 2.0452373038758154 0.0013126121873228852 66-67 1.4063702007030915E-4 0.0 0.0 2.289687884261358 0.001336051690667937 68-69 1.4063702007030915E-4 0.0 0.0 2.560085994849872 0.0013594911940129884 70-71 1.4063702007030915E-4 0.0 0.0 2.8429773607212994 0.0014063702007030914 72-73 1.4063702007030915E-4 0.0 0.0 3.139604275552926 0.0015938862274635034 74-75 1.4063702007030915E-4 0.0 0.0 3.440262784959902 0.0016642047374986581 76-77 1.4063702007030915E-4 0.0 0.0 3.7707129031184383 0.0018282812609140186 78-79 1.4063702007030915E-4 0.0 0.0 4.141947757097364 0.0018282812609140186 80-81 1.4063702007030915E-4 0.0 0.0 4.52905115484089 0.0018985997709491734 82-83 1.4063702007030915E-4 0.0 0.0 4.932843478966093 0.001968918280984328 84-85 1.4063702007030915E-4 0.0 0.0 5.348871223837412 0.001968918280984328 86-87 1.4063702007030915E-4 0.0 0.0 5.78418967996171 0.002062676294364534 88-89 1.4063702007030915E-4 0.0 0.0 6.250870191561685 0.002062676294364534 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2990 0.0 59.650383 1 GTATCAA 5860 0.0 46.993103 1 TATCAAC 7825 0.0 34.949154 2 ATCAACG 7850 0.0 34.70201 3 TCAACGC 7940 0.0 34.308662 4 CAACGCA 8100 0.0 33.689655 5 AACGCAG 8500 0.0 32.01753 6 ACGCAGA 9690 0.0 28.036537 7 CGCAGAG 9725 0.0 27.935637 8 TAAGGTG 1390 0.0 26.67777 5 TGGTATC 1305 0.0 26.605139 2 GTACCGT 240 9.094947E-12 25.72671 6 GCAGAGT 10880 0.0 24.271578 9 AGGTAAG 1305 0.0 24.05396 2 GTAAGGT 1430 0.0 23.604347 4 GGTAAGG 1380 0.0 23.426075 3 GTACATG 7115 0.0 22.393557 1 TACATGG 7015 0.0 22.373783 2 AGAGTAC 9970 0.0 22.104208 10-11 AAGGTAA 1315 0.0 21.700945 1 >>END_MODULE