FastQCFastQC Report
Thu 26 May 2016
SRR938665_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938665_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139414
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54140.2530599500610915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42160.19706330798994492No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29910.139804638092487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28530.13335427364689584No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28100.13134437747906672No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26440.12358524343581935No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26040.1217155725820248No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23790.1111986740294305No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA22460.10498201844056362No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21690.10138290204700913No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA21440.10021435776338755No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.054.546621
GTATCAA55650.048.3649941
TATCAAC73900.036.0743372
ATCAACG73950.036.0499463
TCAACGC75100.035.434644
CAACGCA75850.035.0216145
AACGCAG79100.033.5787476
ACGCAGA90550.029.276157
CGCAGAG90250.029.1102648
GCAGAGT101200.025.1618379
TACATGG71500.024.325122
GTACATG74900.023.786891
TGGTATC14100.022.9176332
TAAGGTG12700.022.824755
ACATGGG75550.022.4550323
GGTAAGG12100.021.9929073
AGGTAAG11600.021.71192
AGAGTAC92550.021.6089310-11
GTAAGGT12900.020.9973774
GAGTACT62850.020.5955612-13
Produced by FastQC (version 0.11.5)