FastQCFastQC Report
Thu 26 May 2016
SRR938665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938665_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139414
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52690.24628239321608628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44470.20786065717060842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30400.14209498488838535No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29120.1361120381562428No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28770.13447607615917256No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27790.12989538256737593No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27120.12676368388727005No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24670.11531194990777849No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22480.10507550198325336No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31150.060.4613231
GTATCAA57700.044.510091
ATCAACG77000.033.15753
TCAACGC77500.032.8208854
TATCAAC78700.032.512332
CAACGCA78950.032.2180985
AACGCAG83500.030.4880546
TAAGGTG14500.028.5265645
ACGCAGA95350.026.5495977
CGCAGAG96450.026.0990728
TGGTATC13500.025.3652552
AGGTAAG12850.024.7977452
GGTAAGG13800.024.4611853
GTAAGGT15250.024.0059624
TACATGG68550.023.727892
GTACATG71900.023.6145571
GCAGAGT107950.022.9667369
ACATGGG71850.021.9689623
AAGGTAA14750.021.603461
GTGGTAT18550.021.5365371
Produced by FastQC (version 0.11.5)