##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938665_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2139414 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76104811878393 31.0 31.0 34.0 30.0 34.0 2 31.998226617195176 31.0 31.0 34.0 30.0 34.0 3 32.06521271712721 33.0 31.0 34.0 30.0 34.0 4 35.745339144270346 37.0 35.0 37.0 33.0 37.0 5 35.60935938532701 37.0 35.0 37.0 33.0 37.0 6 35.6475277809718 37.0 35.0 37.0 33.0 37.0 7 35.5815821528699 37.0 35.0 37.0 33.0 37.0 8 35.56138503347178 37.0 35.0 37.0 33.0 37.0 9 37.13335380622918 39.0 37.0 39.0 33.0 39.0 10-11 37.06260031952675 39.0 37.0 39.0 33.0 39.0 12-13 36.95340242701974 39.0 37.0 39.0 33.0 39.0 14-15 37.99687157324389 40.0 37.0 41.0 33.0 41.0 16-17 37.911469916528546 40.0 37.0 41.0 33.0 41.0 18-19 37.910176805424285 40.0 37.0 41.0 33.0 41.0 20-21 37.89995554857545 40.0 37.0 41.0 32.5 41.0 22-23 37.9259979601891 40.0 37.0 41.0 33.0 41.0 24-25 37.84150683317955 40.0 37.0 41.0 32.0 41.0 26-27 37.66314631015783 40.0 37.0 41.0 32.0 41.0 28-29 37.527473878361086 39.5 36.5 41.0 32.0 41.0 30-31 37.2796814922217 39.0 36.0 41.0 31.0 41.0 32-33 37.05139608322653 39.0 36.0 40.5 31.0 41.0 34-35 36.87639582614679 39.0 35.5 40.5 30.0 41.0 36-37 36.87091722312745 39.0 36.0 41.0 30.0 41.0 38-39 36.715903747474776 39.0 35.0 41.0 30.0 41.0 40-41 36.530939780706305 39.0 35.0 40.0 30.0 41.0 42-43 36.291869409099874 39.0 35.0 40.0 30.0 41.0 44-45 36.00540288134975 38.0 35.0 40.0 29.0 41.0 46-47 35.642337107263955 38.0 34.5 40.0 28.0 41.0 48-49 35.229195704992115 38.0 34.0 40.0 27.0 41.0 50-51 34.84836922633955 38.0 34.0 40.0 26.0 41.0 52-53 34.31952698262234 38.0 33.0 40.0 24.0 41.0 54-55 33.977263400164716 37.0 33.0 40.0 23.5 41.0 56-57 33.59525902887427 37.0 32.0 40.0 23.0 41.0 58-59 33.11590253218872 36.0 31.5 39.0 22.0 40.5 60-61 32.69219188058038 36.0 31.0 39.0 20.5 40.0 62-63 32.20802822642088 35.0 31.0 39.0 18.5 40.0 64-65 31.691953497546525 35.0 30.0 38.0 17.5 40.0 66-67 31.181595988434218 35.0 29.5 38.0 13.5 40.0 68-69 30.59864804100562 34.0 29.0 37.0 9.5 39.5 70-71 30.020479673405895 34.0 28.5 36.5 7.5 39.0 72-73 29.427523611605793 34.0 28.0 36.0 6.5 39.0 74-75 28.805182166705464 33.0 26.5 35.5 2.0 38.0 76-77 27.544468719004364 31.5 26.0 34.5 2.0 36.0 78-79 28.170767088557895 33.0 26.0 35.0 2.0 37.0 80-81 27.87052155403302 33.0 26.0 35.0 2.0 36.5 82-83 27.367301980822788 32.5 26.0 35.0 2.0 36.0 84-85 26.759906217309975 32.0 25.0 35.0 2.0 36.0 86-87 26.11783483701612 32.0 23.5 34.5 2.0 35.0 88-89 25.33817578084466 31.0 20.5 34.0 2.0 35.0 90-91 24.77536933010628 31.0 19.0 34.0 2.0 35.0 92-93 23.98496060136093 31.0 12.0 34.0 2.0 35.0 94-95 23.15981946458236 30.0 3.5 34.0 2.0 35.0 96-97 22.153955475658286 30.0 2.0 34.0 2.0 35.0 98-99 20.94780626844547 29.0 2.0 34.0 2.0 35.0 100-101 17.846778837569538 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32404970029254443 1101 2 0.33898524595059243 1101 3 0.3302882253405457 1101 4 0.22109387848790618 1101 5 0.22270213738340772 1101 6 0.2400881699841122 1101 7 0.31168076894843466 1101 8 0.2831792281422878 1101 9 0.31274200334323154 1101 10-11 0.32464131312307387 1101 12-13 0.3917809354516564 1101 14-15 0.5973511883241969 1101 16-17 0.5722437585917177 1101 18-19 0.5960068035801029 1101 20-21 0.44902916294689277 1101 22-23 0.39349199171611815 1101 24-25 0.5050892960328568 1101 26-27 0.4204524808024246 1101 28-29 0.438506381051333 1101 30-31 0.48888627964728215 1101 32-33 0.49379011595375744 1101 34-35 0.3081108021644283 1101 36-37 0.43841748079245946 1101 38-39 0.5628843834222508 1101 40-41 0.591565637897105 1101 42-43 0.73399641856399 1101 44-45 0.7684796253060426 1101 46-47 0.7837858488254597 1101 48-49 0.9428458067621577 1101 50-51 1.0543619547388374 1101 52-53 0.8197241616870485 1101 54-55 1.0610104706257744 1101 56-57 0.9136605054768623 1101 58-59 1.0922984115080538 1101 60-61 1.0450051435434986 1101 62-63 1.137915041027668 1101 64-65 1.124207934121447 1101 66-67 1.2962835433033 1101 68-69 1.3567472808095324 1101 70-71 1.675619631541597 1101 72-73 1.679645793890245 1101 74-75 1.4782741393000087 1101 76-77 1.3960154373069962 1101 78-79 1.7496285195524415 1101 80-81 2.16721336202432 1101 82-83 2.2326536737119262 1101 84-85 2.3249133556338286 1101 86-87 2.330964155030049 1101 88-89 2.5729615148303964 1101 90-91 2.9923544174436465 1101 92-93 3.1027887636901603 1101 94-95 3.3497385138194815 1101 96-97 3.614252260226735 1101 98-99 3.840769797893831 1101 100-101 4.264100724697212 1102 1 0.3110104734375305 1102 2 0.27355335815028425 1102 3 0.2771661524816267 1102 4 0.199020070229885 1102 5 0.19809041869274324 1102 6 0.167143814145021 1102 7 0.228845840718769 1102 8 0.2324336773967346 1102 9 0.32652962897296334 1102 10-11 0.33744478645287046 1102 12-13 0.3094040652458361 1102 14-15 0.477121637173596 1102 16-17 0.39897520209947857 1102 18-19 0.4751949886893456 1102 20-21 0.528060390004434 1102 22-23 0.43781919229331834 1102 24-25 0.3572386087084851 1102 26-27 0.37069899354893465 1102 28-29 0.23718711065311737 1102 30-31 0.33237837478411336 1102 32-33 0.14309013367481782 1102 34-35 0.4166879584389491 1102 36-37 0.5738088316443424 1102 38-39 0.49239170602168514 1102 40-41 0.46686626319772984 1102 42-43 0.41513819345576763 1102 44-45 0.5011731014205765 1102 46-47 0.5032439005203528 1102 48-49 0.682236107007725 1102 50-51 0.7582389135500023 1102 52-53 0.8653899359712369 1102 54-55 0.7529874142474497 1102 56-57 0.9245906085122328 1102 58-59 1.0374754323429727 1102 60-61 1.0409799289919661 1102 62-63 1.1619231357594444 1102 64-65 1.236671859085373 1102 66-67 1.094969810739567 1102 68-69 1.3883429999052517 1102 70-71 1.6257427296910087 1102 72-73 1.5590428344714944 1102 74-75 1.7605470564413501 1102 76-77 1.7585323672581374 1102 78-79 1.9158315903607779 1102 80-81 2.2727336336630124 1102 82-83 2.327122009956579 1102 84-85 2.502567085919136 1102 86-87 2.528446497249231 1102 88-89 2.5817207867475602 1102 90-91 2.689550374508027 1102 92-93 2.9550670287973766 1102 94-95 3.1907726015640954 1102 96-97 3.6088417959346906 1102 98-99 3.816568670074286 1102 100-101 4.08754823235525 1103 1 0.3352736400547691 1103 2 0.27903763147861227 1103 3 0.38154702926755846 1103 4 0.26617221683770254 1103 5 0.2465441370954764 1103 6 0.22707481915173844 1103 7 0.27589567237447454 1103 8 0.2750256561836082 1103 9 0.41316195805552525 1103 10-11 0.4650994800313626 1103 12-13 0.4858512374105928 1103 14-15 0.6499059035239299 1103 16-17 0.6276926235533438 1103 18-19 0.5601087667539915 1103 20-21 0.608743754448021 1103 22-23 0.5546260368408866 1103 24-25 0.5557915455750191 1103 26-27 0.5492414198001754 1103 28-29 0.5106739745646323 1103 30-31 0.6079524244674843 1103 32-33 0.681156878262513 1103 34-35 0.7707318195418154 1103 36-37 0.7974597876305936 1103 38-39 0.8080698587701463 1103 40-41 0.8564088551765181 1103 42-43 0.9041483856997132 1103 44-45 0.9305805818385551 1103 46-47 0.9609155593658372 1103 48-49 1.298644987387327 1103 50-51 1.275382010561671 1103 52-53 1.4329107173330016 1103 54-55 1.257166038011043 1103 56-57 1.3161672880300443 1103 58-59 1.348452483439388 1103 60-61 1.412214860869227 1103 62-63 1.457994411687082 1103 64-65 1.4446309243820288 1103 66-67 1.5362090384492184 1103 68-69 1.555723676073626 1103 70-71 1.5294953682479573 1103 72-73 1.629668281499736 1103 74-75 1.7731975196062209 1103 76-77 1.774254350580712 1103 78-79 1.9479162248007036 1103 80-81 2.2233957806197324 1103 82-83 2.331651496375642 1103 84-85 2.5590871563969557 1103 86-87 2.7347136151600147 1103 88-89 2.8546426505718543 1103 90-91 3.056435176419999 1103 92-93 3.121385849340612 1103 94-95 3.270698416681938 1103 96-97 3.500397214353125 1103 98-99 3.8490230295809376 1103 100-101 4.248626682541037 1104 1 0.19727610636897452 1104 2 0.22733123851044823 1104 3 0.16592619826895572 1104 4 0.10840464119513626 1104 5 0.0956218974803349 1104 6 0.15630211429049012 1104 7 0.19023130767115504 1104 8 0.1861847292447507 1104 9 0.10133237553124275 1104 10-11 0.20788351353790802 1104 12-13 0.26623752150769775 1104 14-15 0.3928385887051178 1104 16-17 0.42356023315241487 1104 18-19 0.43541660923367687 1104 20-21 0.4938248663289144 1104 22-23 0.3364797043005652 1104 24-25 0.39937129656769343 1104 26-27 0.4510318629903267 1104 28-29 0.5115795513152577 1104 30-31 0.5221131259688292 1104 32-33 0.5871011580640726 1104 34-35 0.5673063130590705 1104 36-37 0.6362105668954712 1104 38-39 0.7165459478223752 1104 40-41 0.59818544243263 1104 42-43 0.5788603599390356 1104 44-45 0.7225726299201227 1104 46-47 0.5939202260191934 1104 48-49 0.745159294848186 1104 50-51 0.7786374191592884 1104 52-53 0.9310409021383634 1104 54-55 0.8261450406991742 1104 56-57 1.0460471156613949 1104 58-59 1.1130311232240047 1104 60-61 1.1100680321224772 1104 62-63 1.0485742688595252 1104 64-65 1.1948770827244424 1104 66-67 1.2644771413111293 1104 68-69 1.413275843454997 1104 70-71 1.3649876901892206 1104 72-73 1.5692566193868878 1104 74-75 1.6125697133327854 1104 76-77 1.4369224904478521 1104 78-79 1.8415351361532153 1104 80-81 2.085937030408161 1104 82-83 2.3385103939773444 1104 84-85 2.362965135869569 1104 86-87 2.5627639969341054 1104 88-89 2.7662277042452033 1104 90-91 2.924842175033355 1104 92-93 3.1431377734546686 1104 94-95 3.502431974850051 1104 96-97 3.849605228928141 1104 98-99 4.271186006062646 1104 100-101 4.792465259858307 1105 1 0.22611331491762598 1105 2 0.23019549433562858 1105 3 0.21298538877083217 1105 4 0.18959447506180993 1105 5 0.20965577878497044 1105 6 0.22740132537952462 1105 7 0.22918972400768212 1105 8 0.22141764827610189 1105 9 0.3490217387736152 1105 10-11 0.2804479694214663 1105 12-13 0.4006979360986591 1105 14-15 0.45317521826534346 1105 16-17 0.39886060934803425 1105 18-19 0.4472942270624358 1105 20-21 0.32514509158852434 1105 22-23 0.3904482714171422 1105 24-25 0.38310148897576823 1105 26-27 0.5224767252048466 1105 28-29 0.5258375336484633 1105 30-31 0.5853373045739403 1105 32-33 0.6131258622452407 1105 34-35 0.6431508593770374 1105 36-37 0.6634210985095379 1105 38-39 0.6734248305742341 1105 40-41 0.6675336110488033 1105 42-43 0.650979003852342 1105 44-45 0.6261117612126554 1105 46-47 0.7459761362280872 1105 48-49 0.8635282468902901 1105 50-51 0.9038262208325136 1105 52-53 0.8025129667825155 1105 54-55 0.9209792324720567 1105 56-57 1.1118437771961212 1105 58-59 1.1773859344587834 1105 60-61 1.180149937159463 1105 62-63 1.2527882553644432 1105 64-65 1.3393564174862895 1105 66-67 1.3767027598423276 1105 68-69 1.508086438183561 1105 70-71 1.430937783149421 1105 72-73 1.651495762163993 1105 74-75 1.6677811256331019 1105 76-77 1.7273483289885725 1105 78-79 2.1520941146867933 1105 80-81 2.34050437505606 1105 82-83 2.400822794943185 1105 84-85 2.475725283155633 1105 86-87 2.6580449121510377 1105 88-89 2.9068003609624924 1105 90-91 2.997376754830757 1105 92-93 3.238597540089014 1105 94-95 3.4297777894331887 1105 96-97 3.841956748324609 1105 98-99 3.9920246672980078 1105 100-101 4.49123906426291 1106 1 0.14327053232216258 1106 2 0.1525896382375116 1106 3 0.12004788792728505 1106 4 0.07993475974415531 1106 5 0.003505861462521409 1106 6 0.10312999414213664 1106 7 0.08318419690199619 1106 8 0.006646198635792189 1106 9 0.024865415832607596 1106 10-11 0.18815401606853044 1106 12-13 0.10880313760652172 1106 14-15 -0.054064946623498145 1106 16-17 -0.002868339164479039 1106 18-19 0.1885963156572643 1106 20-21 0.2950305659016337 1106 22-23 0.26447175404994994 1106 24-25 0.02630491583036587 1106 26-27 0.17558560967754744 1106 28-29 0.3127796544554329 1106 30-31 0.4034705178820275 1106 32-33 0.28280011310647524 1106 34-35 0.12889598594136942 1106 36-37 0.3614580639428411 1106 38-39 0.3194157362482102 1106 40-41 0.35802428175738754 1106 42-43 0.5360741822154083 1106 44-45 -0.0357768328845367 1106 46-47 -0.1355877107585357 1106 48-49 0.3780943625505131 1106 50-51 0.27768447106226546 1106 52-53 0.5263116679085869 1106 54-55 0.6551473916153938 1106 56-57 0.44639890326558174 1106 58-59 1.0003032188724177 1106 60-61 0.9671626089306073 1106 62-63 0.8845172325063473 1106 64-65 0.6187379288801687 1106 66-67 0.912334370927784 1106 68-69 0.6976244663802227 1106 70-71 0.8986925160966379 1106 72-73 1.2957761648239092 1106 74-75 1.8411810808894806 1106 76-77 1.8688402303513705 1106 78-79 1.9261626272393428 1106 80-81 1.8641954910741525 1106 82-83 2.0909921008975623 1106 84-85 2.221281185899187 1106 86-87 2.416629328526877 1106 88-89 2.6362767787403527 1106 90-91 2.6282585069158984 1106 92-93 2.3087688582795884 1106 94-95 2.8288131225830213 1106 96-97 3.3712780357795005 1106 98-99 3.7526767381630783 1106 100-101 4.203737034644593 1107 1 0.17061371967978545 1107 2 0.19535076062745205 1107 3 0.21340764671853663 1107 4 0.1476841942602789 1107 5 0.10807109267320669 1107 6 0.09496055256098401 1107 7 0.19681366595467864 1107 8 0.24047488860417587 1107 9 0.2493305296415187 1107 10-11 0.2469613218546911 1107 12-13 0.2715701582487924 1107 14-15 0.4457218150589455 1107 16-17 0.46411351787572386 1107 18-19 0.34628886784633295 1107 20-21 0.32943676960779555 1107 22-23 0.27172970240765437 1107 24-25 0.2745764784488003 1107 26-27 0.2815153649523765 1107 28-29 0.3093583864679843 1107 30-31 0.26221099558413385 1107 32-33 0.4055569868235267 1107 34-35 0.45096953143475815 1107 36-37 0.49634377222799486 1107 38-39 0.5774892205864575 1107 40-41 0.6459402674696193 1107 42-43 0.7645107591047235 1107 44-45 0.7313023493424851 1107 46-47 0.7191179358725819 1107 48-49 0.7378523491301863 1107 50-51 0.77898442012647 1107 52-53 0.802945938444438 1107 54-55 0.8937447743363336 1107 56-57 0.8356181774345401 1107 58-59 0.7444725590647892 1107 60-61 0.8572141160427051 1107 62-63 0.8882237570355898 1107 64-65 1.1787735678427573 1107 66-67 1.2364173858118157 1107 68-69 1.3654836582794196 1107 70-71 1.304522049362216 1107 72-73 1.3210364563890842 1107 74-75 1.5075130036312245 1107 76-77 1.6045307322339006 1107 78-79 1.8024449740839366 1107 80-81 2.012722416636276 1107 82-83 2.1412708798225353 1107 84-85 2.3503131000309345 1107 86-87 2.675459808892459 1107 88-89 2.797866361766907 1107 90-91 2.7783149144859394 1107 92-93 3.2121338796315833 1107 94-95 3.369460168105782 1107 96-97 3.7548332174303845 1107 98-99 4.142126752285087 1107 100-101 4.253679259130632 1108 1 0.1251575899077011 1108 2 0.17930642973280797 1108 3 0.1547618093914025 1108 4 0.15545859884998237 1108 5 0.1327861822098555 1108 6 0.11134588140034651 1108 7 0.12693464761050421 1108 8 0.12967844055959432 1108 9 0.15119573611664805 1108 10-11 0.10049921909987347 1108 12-13 0.17849979416522643 1108 14-15 0.24660748024891177 1108 16-17 0.34218351598192953 1108 18-19 0.3188672886598809 1108 20-21 0.2729156341253045 1108 22-23 0.3393355947591914 1108 24-25 0.38851975079752776 1108 26-27 0.3846678241459003 1108 28-29 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0.899526875175976 2316 78-79 1.0361734716001898 2316 80-81 1.1306986404537334 2316 82-83 1.3103574204100852 2316 84-85 1.5884784425229377 2316 86-87 1.770663422243281 2316 88-89 1.936623406425248 2316 90-91 2.1605524257254523 2316 92-93 2.1868130313736494 2316 94-95 2.756825478169013 2316 96-97 2.8343580131537145 2316 98-99 3.2196272067791725 2316 100-101 3.4286576399524513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 8.0 4 24.0 5 79.0 6 152.0 7 439.0 8 1046.0 9 1806.0 10 3019.0 11 4540.0 12 6571.0 13 9157.0 14 11767.0 15 15538.0 16 19245.0 17 23501.0 18 22549.0 19 20615.0 20 20989.0 21 22348.0 22 24637.0 23 27623.0 24 31853.0 25 37056.0 26 43488.0 27 51874.0 28 61545.0 29 72447.0 30 86058.0 31 101851.0 32 121220.0 33 146806.0 34 180262.0 35 225124.0 36 280498.0 37 300808.0 38 154507.0 39 8359.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.04185914547588 19.12099253393337 15.268674164361906 23.568474156228834 2 15.900288718648495 20.261651859742784 39.325032477340685 24.51302694426804 3 19.354234081965473 23.61301908514158 30.01739262124704 27.015354211645903 4 13.483224845682043 16.982173623244496 36.06221142798916 33.4723901030843 5 14.000625123995402 37.17003185512096 34.10304064803273 14.726302372850903 6 33.04554424716301 36.05066621046698 16.64708186447317 14.256707677896847 7 29.097173338119692 32.44650170560724 20.05881049670611 18.397514459566967 8 26.721850001916415 35.14453023117545 19.171604934809253 18.962014832098884 9 26.254993189723912 16.766600573801984 19.63939658242865 37.339009654045455 10-11 25.272971944654003 26.3415589502546 27.08316389441221 21.302305210679187 12-13 26.161953693861967 23.523894860929207 27.340500716551354 22.973650728657475 14-15 22.567137543271194 25.153686944181914 25.565528691501505 26.71364682104539 16-17 22.391762417185266 28.182273276701004 26.545376444203878 22.880587861909852 18-19 22.53726955138183 26.58015232208446 28.723449505331832 22.15912862120188 20-21 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26.418671939271558 28.273899670929627 22.65593448507965 46-47 23.122043289444346 26.724222952744515 27.436451026519894 22.717282731291245 48-49 22.809143064409227 26.82278885713564 27.375860866573742 22.992207211881386 50-51 22.91305002210886 26.929173128716556 27.40238682181195 22.755390027362633 52-53 23.37560659133763 26.48257887440206 27.38640113601201 22.755413398248304 54-55 22.7349872441706 26.820218059711674 27.535507386602127 22.909287309515598 56-57 22.82933550963021 26.925854462951072 27.288126561759434 22.95668346565929 58-59 22.71750582168762 26.533106729225853 27.432488522558046 23.316898926528477 60-61 22.386761047651365 26.781913178094563 27.69377502437583 23.137550749878237 62-63 22.53098278313594 26.708318259112072 27.814695986844995 22.94600297090699 64-65 22.304098225027975 26.772915387110675 27.580379487093193 23.342606900768153 66-67 22.195568963204323 26.94019703572342 27.429257332281953 23.434976668790306 68-69 22.845732504351083 26.436020576696244 27.547845539694226 23.170401379258447 70-71 22.609321991909933 26.51665362571246 27.213176131407945 23.660848250969657 72-73 22.629735352641124 26.629430596149504 27.199376651445984 23.541457399763395 74-75 22.639119871142285 27.043643726740125 26.942260824693115 23.374975577424472 76-77 22.587049537864107 26.643744501999144 27.343889494973855 23.42531646516289 78-79 22.65003407475131 26.88577339402285 27.17868070415544 23.285511827070405 80-81 22.921217174991458 26.75906895956975 26.926334466510205 23.393379398928584 82-83 22.8746711975479 26.85327727380553 27.019258971881037 23.25279255676553 84-85 22.694324689305333 26.96806787580534 27.001793561409027 23.335813873480298 86-87 22.86482197549994 26.81238628436009 26.838398463107932 23.48439327703204 88-89 22.696056652909782 26.96131439761411 26.55903124851741 23.783597700958698 90-91 22.544055084719027 26.951515042450076 26.809138375770164 23.695291497060737 92-93 22.71637974955712 26.59387401199396 26.927634254303754 23.76211198414516 94-95 22.400105823370325 26.389726345625487 27.14631202749912 24.06385580350507 96-97 22.725288326616543 25.988822172800592 27.160147591817196 24.125741908765672 98-99 22.385966437538503 26.28119195256271 26.789158152653016 24.54368345724577 100-101 22.444629231801873 25.92850972891414 27.211722373978446 24.415138665305534 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 228.0 1 159.0 2 123.0 3 177.5 4 172.5 5 163.0 6 322.5 7 681.5 8 1080.0 9 1270.0 10 1153.0 11 1028.0 12 1079.0 13 1165.0 14 1347.5 15 1533.5 16 1682.0 17 1907.0 18 2168.0 19 2463.0 20 2853.5 21 3448.5 22 4081.5 23 4956.5 24 6286.0 25 8198.0 26 11157.5 27 14702.0 28 17423.5 29 20004.5 30 23326.0 31 28557.0 32 35907.0 33 38684.5 34 38555.5 35 40942.5 36 45401.0 37 50058.0 38 53422.0 39 55954.0 40 58981.5 41 63364.0 42 66238.0 43 68111.0 44 70117.0 45 74189.5 46 76607.5 47 77672.5 48 79345.5 49 78616.5 50 77307.0 51 76256.5 52 75690.5 53 76706.0 54 78322.0 55 74731.5 56 68418.0 57 62740.5 58 58812.0 59 52931.0 60 43249.0 61 36311.0 62 31029.5 63 23518.0 64 16262.5 65 11860.5 66 8863.0 67 6911.5 68 5424.5 69 4317.5 70 3398.5 71 2416.0 72 1794.0 73 1449.5 74 1114.5 75 854.5 76 641.0 77 436.5 78 279.0 79 167.0 80 99.5 81 57.0 82 37.5 83 22.0 84 13.0 85 10.5 86 4.5 87 2.0 88 2.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.674177134486359E-5 2 0.04674177134486359 3 4.674177134486359E-5 4 0.0 5 0.10460808426980472 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013321404833286125 26-27 0.011989264349957512 28-29 0.0 30-31 0.0 32-33 5.375303704659313E-4 34-35 0.015027479487373646 36-37 0.0035290037365372016 38-39 0.012199602321009398 40-41 0.006987894816057108 42-43 0.005889463189452813 44-45 0.002524055652622634 46-47 4.674177134486359E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.3370885672431796E-4 68-69 1.6359619970702258E-4 70-71 0.0 72-73 4.674177134486359E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.674177134486359E-5 82-83 0.0010049480839145672 84-85 0.004440468277762042 86-87 0.0014723657973632032 88-89 2.3370885672431795E-5 90-91 8.647227698799766E-4 92-93 1.8696708537945436E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.413518842075447E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2139414.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.55315991145778 #Duplication Level Percentage of deduplicated Percentage of total 1 74.24204156347861 38.27411840875108 2 14.09014113492545 14.527825982076417 3 4.8599055489210246 7.516304637543198 4 2.224699759581555 4.587612098427584 5 1.227454415349587 3.163957687927109 6 0.7343966579788826 2.27162810083353 7 0.4752776168271359 1.7151444087838124 8 0.33587418972800553 1.385230065054334 9 0.25948526826140644 1.2039556976413024 >10 1.3989434838947852 13.054663059330688 >50 0.09593364248892434 3.391911684850837 >100 0.04923163722655031 4.595508239714071 >500 0.0035340845504920775 1.2768475005821176 >1k 0.0029903792350317333 2.789144708574356 >5k 9.06175525767192E-5 0.246147719909525 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5269 0.24628239321608628 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4447 0.20786065717060842 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3040 0.14209498488838535 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2912 0.1361120381562428 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2877 0.13447607615917256 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2779 0.12989538256737593 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2712 0.12676368388727005 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2467 0.11531194990777849 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2248 0.10507550198325336 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.674177134486359E-5 0.0 10-11 0.0 0.0 0.0 7.011265701729538E-5 7.011265701729538E-5 12-13 0.0 0.0 0.0 1.402253140345908E-4 1.8696708537945436E-4 14-15 0.0 0.0 0.0 1.402253140345908E-4 2.8045062806918153E-4 16-17 0.0 0.0 0.0 1.402253140345908E-4 3.2719239941404515E-4 18-19 0.0 0.0 0.0 1.402253140345908E-4 3.2719239941404515E-4 20-21 0.0 0.0 0.0 2.1033797105188615E-4 5.141594847934995E-4 22-23 0.0 0.0 0.0 3.038215137416134E-4 5.141594847934995E-4 24-25 0.0 0.0 0.0 5.375303704659314E-4 5.141594847934995E-4 26-27 0.0 0.0 0.0 9.582063125697037E-4 5.141594847934995E-4 28-29 4.674177134486359E-5 0.0 0.0 0.0032018113371231564 5.609012561383632E-4 30-31 4.674177134486359E-5 0.0 0.0 0.012036006121302376 5.609012561383632E-4 32-33 4.674177134486359E-5 0.0 0.0 0.028418996977677066 6.543847988280903E-4 34-35 4.674177134486359E-5 0.0 0.0 0.056113496499508746 6.777556845005222E-4 36-37 4.674177134486359E-5 0.0 0.0 0.09948986030754216 7.011265701729539E-4 38-39 4.674177134486359E-5 0.0 0.0 0.16864431101226784 7.011265701729539E-4 40-41 4.674177134486359E-5 0.0 0.0 0.23955157814242595 7.011265701729539E-4 42-43 4.674177134486359E-5 0.0 0.0 0.31695595148952005 7.244974558453857E-4 44-45 4.674177134486359E-5 0.0 0.0 0.41621210294033784 7.946101128626811E-4 46-47 4.674177134486359E-5 0.0 0.0 0.5345856388712049 8.880936555524083E-4 48-49 4.674177134486359E-5 0.0 0.0 0.6563245823389021 9.348354268972718E-4 50-51 4.674177134486359E-5 0.0 0.0 0.7952644976615092 9.348354268972718E-4 52-53 4.674177134486359E-5 0.0 0.0 0.9319608079595627 9.815771982421356E-4 54-55 4.674177134486359E-5 0.0 0.0 1.0770238953283469 0.0010049480839145674 56-57 4.674177134486359E-5 0.0 0.0 1.2427234747458882 0.0010516898552594307 58-59 4.674177134486359E-5 0.0 0.0 1.4281247107852897 0.0010750607409318626 60-61 7.011265701729538E-5 0.0 0.0 1.6296051161673244 0.0010750607409318626 62-63 9.348354268972718E-5 0.0 0.0 1.8318801316622215 0.0011218025122767263 64-65 9.348354268972718E-5 0.0 0.0 2.038829324291605 0.0011451733979491582 66-67 9.348354268972718E-5 0.0 0.0 2.2821669859129647 0.0012386569406388852 68-69 9.348354268972718E-5 0.0 0.0 2.5506984622892066 0.001262027826311317 70-71 9.348354268972718E-5 0.0 0.0 2.8348650611803046 0.0014256240260183397 72-73 9.348354268972718E-5 0.0 0.0 3.1315350839061535 0.0014489949116907714 74-75 9.348354268972718E-5 0.0 0.0 3.428532299031417 0.0014489949116907714 76-77 9.348354268972718E-5 0.0 0.0 3.7527565959650633 0.0015424784543804986 78-79 9.348354268972718E-5 0.0 0.0 4.129027855291215 0.0015424784543804986 80-81 9.348354268972718E-5 0.0 0.0 4.524112677583675 0.0015658493400529305 82-83 9.348354268972718E-5 0.0 0.0 4.9309764262550395 0.0016359619970702259 84-85 9.348354268972718E-5 0.0 0.0 5.344150314057962 0.0016359619970702259 86-87 9.348354268972718E-5 0.0 0.0 5.772141343377205 0.0016593328827426575 88-89 9.348354268972718E-5 0.0 0.0 6.226307764649572 0.0017528164254323848 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3115 0.0 60.461323 1 GTATCAA 5770 0.0 44.51009 1 ATCAACG 7700 0.0 33.1575 3 TCAACGC 7750 0.0 32.820885 4 TATCAAC 7870 0.0 32.51233 2 CAACGCA 7895 0.0 32.218098 5 AACGCAG 8350 0.0 30.488054 6 TAAGGTG 1450 0.0 28.526564 5 ACGCAGA 9535 0.0 26.549597 7 CGCAGAG 9645 0.0 26.099072 8 TGGTATC 1350 0.0 25.365255 2 AGGTAAG 1285 0.0 24.797745 2 GGTAAGG 1380 0.0 24.461185 3 GTAAGGT 1525 0.0 24.005962 4 TACATGG 6855 0.0 23.72789 2 GTACATG 7190 0.0 23.614557 1 GCAGAGT 10795 0.0 22.966736 9 ACATGGG 7185 0.0 21.968962 3 AAGGTAA 1475 0.0 21.60346 1 GTGGTAT 1855 0.0 21.536537 1 >>END_MODULE