FastQCFastQC Report
Thu 26 May 2016
SRR938664_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938664_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144831
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49790.2321395019001497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38940.1815527656957588No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29230.1362811335718292No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28560.13315734433155807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26980.12579079657091863No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26320.12271363105065154No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25900.12075543481048158No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22600.10536960720914608No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21990.10252556028889923No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21740.10135996728879804No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA21540.10042749288871711No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31250.059.688991
GTATCAA58050.046.2672541
ATCAACG77550.034.3209573
TATCAAC77500.034.2817732
TCAACGC78750.033.8583224
CAACGCA80750.032.9601025
AACGCAG84900.031.3409356
TGGTATC12300.028.9807322
ACGCAGA97000.027.3264147
CGCAGAG98700.026.4707168
TAAGGTG14650.024.3313795
AGGTAAG12950.024.222972
GTACATG77050.023.7753071
TACATGG74850.023.7483232
GCAGAGT110150.023.1579349
ACATGGG75200.022.816163
GTGGTAT18150.022.6042121
AAGGTAA13300.022.5972941
GTAAGGT15300.022.3663064
GGTAAGG14500.022.2891793