##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938664_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2144831 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.04446644047946 31.0 31.0 34.0 28.0 34.0 2 31.213165046570104 31.0 31.0 34.0 28.0 34.0 3 31.268708816685322 31.0 31.0 34.0 27.0 34.0 4 34.94512201660643 37.0 35.0 37.0 32.0 37.0 5 34.933614350035036 37.0 35.0 37.0 32.0 37.0 6 34.904446084563304 37.0 35.0 37.0 32.0 37.0 7 34.81933541617032 37.0 35.0 37.0 32.0 37.0 8 34.771044431938925 37.0 35.0 37.0 32.0 37.0 9 36.253728615448026 39.0 35.0 39.0 32.0 39.0 10-11 36.08921448822774 38.5 35.0 39.0 31.0 39.0 12-13 35.90158921612006 38.0 35.0 39.0 30.0 39.0 14-15 37.05137048093766 39.0 36.0 41.0 31.0 41.0 16-17 36.973665524230114 39.0 36.0 41.0 31.0 41.0 18-19 36.959947660211924 39.0 36.0 41.0 31.0 41.0 20-21 36.88519188691323 39.0 36.0 41.0 30.0 41.0 22-23 36.731168796049666 39.0 36.0 41.0 30.0 41.0 24-25 36.58598206571986 39.0 36.0 41.0 30.0 41.0 26-27 36.368032026765746 39.0 35.5 40.5 29.5 41.0 28-29 36.132201091834276 39.0 35.0 40.0 29.0 41.0 30-31 35.847896174570394 38.5 35.0 40.0 27.0 41.0 32-33 35.5460329974716 38.0 34.5 40.0 27.0 41.0 34-35 35.239364080433376 38.0 34.0 40.0 26.0 41.0 36-37 34.99720537422296 38.0 34.0 40.0 25.0 41.0 38-39 34.73937410453318 38.0 33.5 40.0 24.5 41.0 40-41 34.45363154486297 38.0 33.0 40.0 24.0 41.0 42-43 34.02455461525874 38.0 33.0 40.0 23.0 41.0 44-45 33.553074111666604 37.5 32.5 40.0 21.0 41.0 46-47 33.06719480462563 37.0 31.5 40.0 18.5 41.0 48-49 32.557989184229434 37.0 31.0 39.5 14.5 41.0 50-51 31.823455554307078 36.0 30.5 38.5 13.0 40.5 52-53 31.816080847395437 36.0 30.0 39.0 10.0 40.0 54-55 31.778096735826736 36.0 30.0 39.0 8.0 40.0 56-57 31.33708226895266 36.0 29.5 39.0 6.5 40.0 58-59 30.87430315022489 35.0 29.0 39.0 2.0 40.0 60-61 30.307216512629665 35.0 28.0 38.5 2.0 40.0 62-63 29.56946444731543 34.0 27.5 38.0 2.0 40.0 64-65 28.90691084752132 34.0 26.0 38.0 2.0 40.0 66-67 28.198822424703856 34.0 25.5 37.0 2.0 39.5 68-69 27.343749694031835 33.0 23.5 36.5 2.0 39.0 70-71 26.496370809634882 32.0 21.5 36.0 2.0 39.0 72-73 25.582959449952 31.5 18.5 35.0 2.0 38.0 74-75 24.643002642166213 31.0 10.5 35.0 2.0 37.5 76-77 23.683242642427306 30.0 6.0 34.5 2.0 36.5 78-79 22.68661913223 29.5 2.0 34.0 2.0 36.0 80-81 21.71602284748775 29.0 2.0 34.0 2.0 35.5 82-83 20.698309796902414 27.5 2.0 33.5 2.0 35.0 84-85 19.708696862363517 26.0 2.0 33.0 2.0 35.0 86-87 18.709483870757182 25.0 2.0 32.0 2.0 34.5 88-89 17.597907014585296 21.5 2.0 32.0 2.0 34.0 90-91 16.42777729340913 16.5 2.0 31.0 2.0 34.0 92-93 15.265176137420617 4.5 2.0 31.0 2.0 34.0 94-95 14.011220464456175 2.0 2.0 30.0 2.0 34.0 96-97 12.599219472303412 2.0 2.0 29.0 2.0 34.0 98-99 10.904975030666751 2.0 2.0 27.5 2.0 33.5 100-101 7.705570508818644 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.45899937633042853 1101 2 0.4003174156443734 1101 3 0.3606621011002282 1101 4 0.2206210823240795 1101 5 0.2505305496972241 1101 6 0.44689799502597083 1101 7 0.3333249905510627 1101 8 0.3603523874848733 1101 9 0.4330643844246822 1101 10-11 0.524382749111659 1101 12-13 0.5623023592088003 1101 14-15 0.5743191937616601 1101 16-17 0.4307309234017396 1101 18-19 0.4365922075985438 1101 20-21 0.6172541576965926 1101 22-23 0.6682546018080444 1101 24-25 0.6837888569877535 1101 26-27 0.6277023238839732 1101 28-29 0.7897778848560435 1101 30-31 0.8122069836425325 1101 32-33 0.7661585537448801 1101 34-35 0.8822094674180434 1101 36-37 0.9346680333472506 1101 38-39 1.080116336225096 1101 40-41 0.9492566633783284 1101 42-43 0.9935596730476632 1101 44-45 1.1119741482029184 1101 46-47 1.077089831159057 1101 48-49 0.8710618716994247 1101 50-51 1.0061037157068462 1101 52-53 1.0957856258603726 1101 54-55 1.1816724780964414 1101 56-57 1.3275621720146802 1101 58-59 1.3254100521041146 1101 60-61 1.3930659597372461 1101 62-63 1.1302428523328025 1101 64-65 1.244842260827781 1101 66-67 1.6716784050421403 1101 68-69 1.6892731166760768 1101 70-71 1.8639210642208823 1101 72-73 2.038726533241075 1101 74-75 2.2183396205366144 1101 76-77 2.364068376506207 1101 78-79 2.6276772322410302 1101 80-81 2.964289511370385 1101 82-83 2.982052960316846 1101 84-85 2.8796473224008032 1101 86-87 3.084337823992726 1101 88-89 3.1933832252361682 1101 90-91 3.624746612412981 1101 92-93 3.6199371166120784 1101 94-95 3.2190453084683757 1101 96-97 3.078557023773266 1101 98-99 2.741660125240225 1101 100-101 2.2104312337753784 1102 1 0.5701994533369756 1102 2 0.44122364267366976 1102 3 0.537050844143387 1102 4 0.2824678392984268 1102 5 0.41467350184816354 1102 6 0.5051298197310672 1102 7 0.5321068870122616 1102 8 0.6157025922522763 1102 9 0.6397359515078875 1102 10-11 0.7183246091697626 1102 12-13 0.7960089117657745 1102 14-15 0.8846078808000613 1102 16-17 0.9367667489469085 1102 18-19 0.8225630151171828 1102 20-21 0.7158802909179158 1102 22-23 0.5703390289843568 1102 24-25 0.6935669381246541 1102 26-27 0.6496929430866061 1102 28-29 0.64625649803817 1102 30-31 0.532701663190295 1102 32-33 0.6167789814812394 1102 34-35 0.7504766451281455 1102 36-37 0.9462745824039231 1102 38-39 1.0054720539611068 1102 40-41 1.1034290180284714 1102 42-43 1.3564083901886193 1102 44-45 1.2937039077324712 1102 46-47 1.414001781174818 1102 48-49 1.3469076310889676 1102 50-51 1.5733050528204977 1102 52-53 1.4326888251323169 1102 54-55 1.3553734301773694 1102 56-57 1.7817235882350673 1102 58-59 1.8376912835087857 1102 60-61 1.799734464060112 1102 62-63 1.864498951601238 1102 64-65 1.8833831618043675 1102 66-67 2.118946422823651 1102 68-69 2.014483572064787 1102 70-71 1.8918039967701503 1102 72-73 2.202567147193264 1102 74-75 2.2903595547310225 1102 76-77 2.3392337655642805 1102 78-79 2.498667203888619 1102 80-81 2.8593829693143107 1102 82-83 2.9580409192933566 1102 84-85 2.9097525063414373 1102 86-87 3.1088455946532356 1102 88-89 3.2597178011751247 1102 90-91 3.2726022426415504 1102 92-93 3.270841068447986 1102 94-95 3.0948298126512324 1102 96-97 2.818952644901062 1102 98-99 2.7017370442783424 1102 100-101 2.0772295991364347 1103 1 0.487379121290445 1103 2 0.4351028781584567 1103 3 0.3703139622167946 1103 4 0.3562441592736647 1103 5 0.4428149182475778 1103 6 0.5185875057226923 1103 7 0.4157702320672456 1103 8 0.4071645599417977 1103 9 0.45842453106750725 1103 10-11 0.5198234183762764 1103 12-13 0.5017017214365609 1103 14-15 0.508504569792315 1103 16-17 0.4631404603535927 1103 18-19 0.61922170017165 1103 20-21 0.4919410428803559 1103 22-23 0.6125399764604751 1103 24-25 0.5726059634795249 1103 26-27 0.750779273465163 1103 28-29 0.6609647321387158 1103 30-31 0.76018620866828 1103 32-33 0.9284691774248088 1103 34-35 0.7783096159214651 1103 36-37 0.8551883380032237 1103 38-39 0.8226332366726723 1103 40-41 0.9807994278849819 1103 42-43 0.9684743094210049 1103 44-45 1.1098195995534041 1103 46-47 1.2671404032934817 1103 48-49 1.0759580485115237 1103 50-51 1.1267123557807146 1103 52-53 1.2880542740657575 1103 54-55 1.207243018945217 1103 56-57 1.3748720118308313 1103 58-59 1.5244337110079478 1103 60-61 1.6085998571079436 1103 62-63 1.7826929434677332 1103 64-65 1.8173061600246214 1103 66-67 1.914644735078074 1103 68-69 2.1807765932411343 1103 70-71 2.164684642291995 1103 72-73 2.431725466841783 1103 74-75 2.3677946629341697 1103 76-77 2.3185818813592753 1103 78-79 2.6838990002242404 1103 80-81 2.930365868316109 1103 82-83 2.8984904975719097 1103 84-85 2.963956609205365 1103 86-87 3.107103683021286 1103 88-89 3.15984225414093 1103 90-91 3.0440911214041577 1103 92-93 3.0298392973038766 1103 94-95 2.9348682017698753 1103 96-97 2.93262463073191 1103 98-99 2.535295711277458 1103 100-101 1.9382420745252693 1104 1 0.5528302840045605 1104 2 0.5838065944393342 1104 3 0.6073868327695351 1104 4 0.42776358037601625 1104 5 0.37707706454025214 1104 6 0.5022664941336288 1104 7 0.5297380312971143 1104 8 0.46611238547396283 1104 9 0.6127554585015531 1104 10-11 0.5411637862380729 1104 12-13 0.5700283351281641 1104 14-15 0.756003895770057 1104 16-17 0.6460832723950247 1104 18-19 0.6130212099362282 1104 20-21 0.6221683170108747 1104 22-23 0.6559063626103949 1104 24-25 0.7011920191424466 1104 26-27 0.7601332617217409 1104 28-29 0.8441468992591865 1104 30-31 0.8579008101513423 1104 32-33 0.8645862741279657 1104 34-35 0.8137065893042745 1104 36-37 1.0162635012606813 1104 38-39 1.10241637644328 1104 40-41 1.1414775868883709 1104 42-43 1.1015718893209439 1104 44-45 1.0156393443922411 1104 46-47 1.0951732461855812 1104 48-49 1.2590576864946854 1104 50-51 1.1801837247559384 1104 52-53 1.3853618895911168 1104 54-55 1.6187847496697252 1104 56-57 1.6869735411924722 1104 58-59 1.8000075560630968 1104 60-61 1.942706823925466 1104 62-63 1.9673230750409942 1104 64-65 2.014671710592758 1104 66-67 2.345806173184311 1104 68-69 2.5746296131695523 1104 70-71 2.3822966894879585 1104 72-73 2.6743274808820274 1104 74-75 2.563489873157085 1104 76-77 2.849475459933803 1104 78-79 3.170287455200583 1104 80-81 3.2578577440325773 1104 82-83 3.1891904310958203 1104 84-85 3.2788178223630986 1104 86-87 3.1496304741196646 1104 88-89 3.2974820237179294 1104 90-91 3.1056407757490803 1104 92-93 3.2439875904325532 1104 94-95 3.56409061676589 1104 96-97 3.320303812602665 1104 98-99 3.118046709962341 1104 100-101 2.306225605743305 1105 1 0.5012589154436569 1105 2 0.3720484396373074 1105 3 0.29807194360059697 1105 4 0.37653999372849256 1105 5 0.43032872773886766 1105 6 0.4031076183679687 1105 7 0.3877531344105378 1105 8 0.37555036599427183 1105 9 0.4030077957718561 1105 10-11 0.6008542994117789 1105 12-13 0.578932431334799 1105 14-15 0.6799676326671786 1105 16-17 0.7386093804614191 1105 18-19 0.5594676798521121 1105 20-21 0.5959908896201114 1105 22-23 0.6799296913522284 1105 24-25 0.7999967630440494 1105 26-27 0.6164827239297708 1105 28-29 0.7683905224433474 1105 30-31 0.8522972229176773 1105 32-33 0.8746157769756664 1105 34-35 0.862682293416114 1105 36-37 1.0610931760455102 1105 38-39 0.9881109145087876 1105 40-41 1.0237153095029825 1105 42-43 1.1081255268657628 1105 44-45 0.9831378545961229 1105 46-47 1.389939421463751 1105 48-49 1.2629536254570084 1105 50-51 0.9946269317740963 1105 52-53 1.1812461446442057 1105 54-55 1.4039307496892626 1105 56-57 1.554902382668402 1105 58-59 1.4172662335149049 1105 60-61 1.5932485235630303 1105 62-63 1.9114044325627546 1105 64-65 1.9539622745660665 1105 66-67 2.199041717277389 1105 68-69 2.3783951339143563 1105 70-71 2.3439537335370773 1105 72-73 2.4384331635994165 1105 74-75 2.4095939341947634 1105 76-77 2.6908702998662015 1105 78-79 3.0271810593348896 1105 80-81 3.1676737908463686 1105 82-83 3.3290607944106902 1105 84-85 3.5030604686317695 1105 86-87 3.5288553893722465 1105 88-89 3.591988339476554 1105 90-91 3.076089570723827 1105 92-93 3.0986386850663585 1105 94-95 3.142357666383427 1105 96-97 3.1546248074934002 1105 98-99 2.7952572254806434 1105 100-101 1.8954824661323117 1106 1 0.2499119066607456 1106 2 0.2935010207880566 1106 3 0.15256739442713751 1106 4 0.17285728255911437 1106 5 0.08326277211671851 1106 6 -0.11758594575911019 1106 7 0.22994659503530102 1106 8 0.09720403736947247 1106 9 0.11578392517932912 1106 10-11 0.11211535198109601 1106 12-13 0.3871815663511171 1106 14-15 0.2860466690112773 1106 16-17 -0.08090376433340651 1106 18-19 0.3392699411313984 1106 20-21 0.38189683065336055 1106 22-23 0.1859062111113161 1106 24-25 0.33154082053219014 1106 26-27 0.20566328126512445 1106 28-29 0.3476530930467092 1106 30-31 0.16134135433605223 1106 32-33 0.30762463874585677 1106 34-35 0.23816308634246752 1106 36-37 0.2663501082367077 1106 38-39 0.3232469954180388 1106 40-41 0.5731706901450977 1106 42-43 0.1151967068907993 1106 44-45 0.7786483181879547 1106 46-47 0.6398250760420083 1106 48-49 0.4280013843853183 1106 50-51 0.3684127509703252 1106 52-53 0.504967890974811 1106 54-55 0.28792337638103405 1106 56-57 0.6784683826486813 1106 58-59 0.8679907497647683 1106 60-61 1.1393839440288893 1106 62-63 1.1441634101569669 1106 64-65 1.2158044187398716 1106 66-67 1.4589956833877729 1106 68-69 1.4612911288673835 1106 70-71 1.3127798200650176 1106 72-73 1.4836355845680025 1106 74-75 1.9193768750407223 1106 76-77 2.173826917607485 1106 78-79 2.317582944062874 1106 80-81 2.2760840937631635 1106 82-83 2.4041211707648458 1106 84-85 2.4322525590829436 1106 86-87 2.2604985133680273 1106 88-89 2.0727876731187536 1106 90-91 1.872034744065573 1106 92-93 2.178212748370349 1106 94-95 2.021204256228371 1106 96-97 1.9388599253381837 1106 98-99 1.9323603433648593 1106 100-101 1.1934370631139872 1107 1 0.36066346169836194 1107 2 0.3022706795261598 1107 3 0.3068797678983941 1107 4 0.2575957515038638 1107 5 0.2893766604592969 1107 6 0.2808115117776211 1107 7 0.2553283705912932 1107 8 0.36705524103756915 1107 9 0.4385252923599907 1107 10-11 0.5118756261882638 1107 12-13 0.4468467721581604 1107 14-15 0.4050021299159212 1107 16-17 0.5294792979140865 1107 18-19 0.4155320974306633 1107 20-21 0.5076147115556324 1107 22-23 0.49538673021061186 1107 24-25 0.4304502149457008 1107 26-27 0.48035156434900017 1107 28-29 0.5485862623880351 1107 30-31 0.5883581576464181 1107 32-33 0.7749946315345113 1107 34-35 0.5571326372528915 1107 36-37 0.5529819217085148 1107 38-39 0.7988171758777369 1107 40-41 0.790753353958543 1107 42-43 0.906797595735334 1107 44-45 0.7543330777919053 1107 46-47 0.6775513600671701 1107 48-49 0.5785821568111729 1107 50-51 0.8176183779706925 1107 52-53 0.8800137079554133 1107 54-55 0.9991439133472433 1107 56-57 1.19214048277383 1107 58-59 1.1222450998094367 1107 60-61 1.2549290364505232 1107 62-63 1.4069742097550098 1107 64-65 1.4226334727231844 1107 66-67 1.506936145285561 1107 68-69 1.7743960084125128 1107 70-71 1.7285158124955515 1107 72-73 1.5693761073267858 1107 74-75 1.9880052814708513 1107 76-77 2.1073521294223454 1107 78-79 2.5358989731078587 1107 80-81 2.518093152462715 1107 82-83 2.8309946278358247 1107 84-85 2.9159705789283237 1107 86-87 3.108598074914447 1107 88-89 3.2306041527503773 1107 90-91 2.990025039574089 1107 92-93 2.979960104781938 1107 94-95 2.880898522672341 1107 96-97 2.519321392597023 1107 98-99 2.2695695046083415 1107 100-101 1.7915640708175031 1108 1 0.35796332704842726 1108 2 0.2697415314472771 1108 3 0.16088952366035514 1108 4 0.1899150842813242 1108 5 0.09571404500095326 1108 6 0.22840416160014598 1108 7 0.20401190628377464 1108 8 0.35863426814376 1108 9 0.36949080686307667 1108 10-11 0.2487168839968774 1108 12-13 0.32135272180430974 1108 14-15 0.30117072722368476 1108 16-17 0.3375569400050793 1108 18-19 0.31536637865635697 1108 20-21 0.4091455197498277 1108 22-23 0.39190773378901866 1108 24-25 0.4332133838429044 1108 26-27 0.3108980337633014 1108 28-29 0.4395104835125423 1108 30-31 0.5718689840757136 1108 32-33 0.7202380891925984 1108 34-35 0.6959367943294694 1108 36-37 0.5219155524098014 1108 38-39 0.3561154896155685 1108 40-41 0.4524140584453846 1108 42-43 0.5797909247175639 1108 44-45 0.678731503271429 1108 46-47 0.8237694973530125 1108 48-49 0.7300965431659137 1108 50-51 0.8219469735804701 1108 52-53 0.7348921287069174 1108 54-55 0.9876316092268489 1108 56-57 1.283816430753106 1108 58-59 1.4601364770720586 1108 60-61 1.4844599683345585 1108 62-63 1.4792061509005983 1108 64-65 1.590796676714632 1108 66-67 1.7945444905441654 1108 68-69 1.817643448664004 1108 70-71 1.7322186399612107 1108 72-73 1.9474651285174538 1108 74-75 2.1283311346791827 1108 76-77 2.199016275633987 1108 78-79 2.5426223211943153 1108 80-81 2.7090657882804727 1108 82-83 3.103846555174261 1108 84-85 2.7940148631858825 1108 86-87 2.7376266174437163 1108 88-89 2.772820356376066 1108 90-91 2.8754087424857246 1108 92-93 3.0860861736424177 1108 94-95 3.021775308009895 1108 96-97 2.707626737443759 1108 98-99 2.491612992566788 1108 100-101 1.6575081582508533 1109 1 0.4919996157913431 1109 2 0.4844495756192302 1109 3 0.37449377504426096 1109 4 0.3678268727882994 1109 5 0.24133562477196335 1109 6 0.45107406240428105 1109 7 0.48583540148844406 1109 8 0.33310154843628226 1109 9 0.36074438232940054 1109 10-11 0.41202413394405824 1109 12-13 0.3702830021613508 1109 14-15 0.5384320939700658 1109 16-17 0.553379108549926 1109 18-19 0.5032769273731503 1109 20-21 0.5435740924434924 1109 22-23 0.7636928714036131 1109 24-25 0.584642684807875 1109 26-27 0.5897365443451648 1109 28-29 0.7652015716561422 1109 30-31 0.6955668354012587 1109 32-33 0.6693910889998946 1109 34-35 0.7873922995671663 1109 36-37 0.6892516987455224 1109 38-39 0.8895561941200114 1109 40-41 0.670852015591656 1109 42-43 0.8769889778252846 1109 44-45 0.833274617396647 1109 46-47 1.0656174552203908 1109 48-49 1.2452464418109628 1109 50-51 1.1870907997838138 1109 52-53 1.5007105230605546 1109 54-55 1.4025160072264917 1109 56-57 1.735363406360861 1109 58-59 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-0.7682153379145458 2315 92-93 -0.6710488288769838 2315 94-95 -0.8198744517047967 2315 96-97 -0.7663605284756709 2315 98-99 -0.581537367509565 2315 100-101 -0.010628549059382486 2316 1 -0.9987104437281324 2316 2 -0.9106410738851807 2316 3 -0.7743028787320192 2316 4 -0.9151077118665185 2316 5 -0.7754527023377733 2316 6 -0.6849517497193105 2316 7 -0.7930095652243381 2316 8 -0.7175325164576307 2316 9 -0.865573889710916 2316 10-11 -0.9382590143246077 2316 12-13 -1.0051899856928017 2316 14-15 -1.103498971795311 2316 16-17 -1.2057090192570783 2316 18-19 -1.1957233706538588 2316 20-21 -1.1144825408281704 2316 22-23 -1.1747027076664764 2316 24-25 -1.0894646700373656 2316 26-27 -1.1936676928843397 2316 28-29 -1.1556058915913425 2316 30-31 -1.0588682948826715 2316 32-33 -1.3132412628040342 2316 34-35 -0.9763013404279661 2316 36-37 -1.1969893802298301 2316 38-39 -1.3490857660022542 2316 40-41 -1.230541294583908 2316 42-43 -1.0813920481202501 2316 44-45 -1.1736389081904903 2316 46-47 -0.9728326641486973 2316 48-49 -1.055983790157164 2316 50-51 -0.8187952336033497 2316 52-53 -0.6370665934399682 2316 54-55 -0.6100813998933283 2316 56-57 -0.5175491405761292 2316 58-59 -0.4566662301460589 2316 60-61 -0.6713575638799618 2316 62-63 -0.4610328575228628 2316 64-65 -0.46612881765096503 2316 66-67 -0.37701899900633506 2316 68-69 -0.21742931193143988 2316 70-71 -0.3600635755656221 2316 72-73 -0.21521408337159187 2316 74-75 -0.2027271381946889 2316 76-77 0.07608026861255368 2316 78-79 -0.10941496210183033 2316 80-81 -0.13898119935845088 2316 82-83 0.06238783484274535 2316 84-85 0.25883834041868425 2316 86-87 0.38844646280941575 2316 88-89 0.11074260201112551 2316 90-91 -0.10324042308869963 2316 92-93 0.01488000432204295 2316 94-95 0.08731774434951056 2316 96-97 0.12559232623866556 2316 98-99 0.03661443625593641 2316 100-101 0.3390800156567879 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6996.0 3 2566.0 4 2861.0 5 3386.0 6 4012.0 7 5194.0 8 6811.0 9 8951.0 10 11443.0 11 14425.0 12 17597.0 13 20923.0 14 24208.0 15 27689.0 16 31493.0 17 36001.0 18 36221.0 19 36676.0 20 40299.0 21 44035.0 22 48700.0 23 54985.0 24 60208.0 25 67153.0 26 74085.0 27 81230.0 28 90348.0 29 99253.0 30 110460.0 31 122484.0 32 136362.0 33 152969.0 34 169518.0 35 181242.0 36 172345.0 37 114861.0 38 26513.0 39 328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.509028109292075 18.281195885166568 14.569468235470314 23.640307770071047 2 15.601675580586246 19.502409276694653 40.28235081232639 24.613564330392705 3 19.45637391072365 23.33583945405124 29.502808658615987 27.704977976609126 4 13.07081773479895 16.131364458570324 36.47967393971168 34.318143866919044 5 14.222085787104922 36.955841208806845 33.943434684616385 14.878638319471845 6 33.23166920127259 35.3737533004301 17.035350755250366 14.359226743046937 7 29.332824017327937 32.24408736355275 20.058608653148337 18.364479965970975 8 26.796127825598237 34.66295711550526 19.42047980074766 19.120435258148845 9 26.542187538623775 16.80547516628336 19.758535241905506 36.89380205318736 10-11 25.377966538854935 26.098615743463622 27.133041889629805 21.390375828051635 12-13 26.346330753903246 23.423246117738138 27.238898287595763 22.991524840762846 14-15 22.87557741230678 24.95433167075898 25.515946023462583 26.654144893471653 16-17 22.664934998315353 27.97755048982975 26.46871396810481 22.88880054375008 18-19 22.79942936077774 26.45133850247778 28.580649193055702 22.168582943688783 20-21 23.273252439331245 25.733536714905657 28.74501272456294 22.24819812120015 22-23 22.873088889686944 25.68029487334354 28.719089112623575 22.72752712434594 24-25 22.63036375212521 25.966962155505957 28.82690697582216 22.575767116546675 26-27 22.26399694035559 26.24127814779575 28.54349732281114 22.95122758903752 28-29 22.425530356965048 26.66773313658052 28.4404673097045 22.46626919674994 30-31 22.559035782696643 26.20537656926207 28.19863148826933 23.036956159771947 32-33 22.142018835449935 26.2411438477214 28.234986310754845 23.381851006073823 34-35 23.00480957017017 26.42546915170842 27.970804451621557 22.598916826499856 36-37 23.16963113486811 25.888162819473003 28.06262438103891 22.87958166461998 38-39 22.545457173719882 26.28939782986251 28.080289351665655 23.084855644751954 40-41 23.099181444108012 26.089105529105026 28.133571796111063 22.678141230675898 42-43 22.839681464985023 26.107752237280422 27.8774559724849 23.175110325249655 44-45 22.794341876659402 26.40588119545015 28.106782716387873 22.692994211502576 46-47 23.25529122858723 26.708008494691747 27.25937535849222 22.7773249182288 48-49 22.90924199639489 26.821214056435306 27.25559005537769 23.013953891792113 50-51 23.08226926147258 26.881290240754417 27.27943123913546 22.757009258637538 52-53 23.42654644432917 26.45020428860355 27.357676996695247 22.765572270372033 54-55 22.834422628492455 26.785540265375523 27.470544268342895 22.90949283778913 56-57 22.894534992970865 26.911796591044208 27.264302483627826 22.929365932357104 58-59 22.79877505479662 26.447453757992196 27.402810305541582 23.350960881669604 60-61 22.501921612209912 26.632297959116112 27.6974667823947 23.168313646279277 62-63 22.69165237486396 26.586506673082823 27.870414149863894 22.851426802189327 64-65 22.468280800996325 26.631794315139935 27.59743998985396 23.30248489400978 66-67 22.35136140099755 26.79242291960746 27.479446977105802 23.376768702289187 68-69 23.008617250961027 26.25047821161794 27.596721141021664 23.144183396399374 70-71 22.802269091750123 26.296228337907312 27.28587259335663 23.61562997698594 72-73 22.792059988627283 26.458536799175047 27.248448608171245 23.500954604026425 74-75 22.861486021771114 26.82812758645612 27.057729117754718 23.252657274018052 76-77 22.94829995885258 26.445667340023384 27.199446083476804 23.40658661764723 78-79 23.01755477225564 26.69100807808152 26.955995450032066 23.335441699630778 80-81 23.239649405689768 26.575279995635526 26.716612569226616 23.468458029448094 82-83 23.215764558421135 26.612286503535504 26.806711207853272 23.365237730190085 84-85 23.10838951178139 26.67176150558552 26.811387757652284 23.40846122498081 86-87 23.288943512313118 26.539524222929465 26.605661357356862 23.56587090740055 88-89 23.207963461785518 26.56087483553817 26.374560531631115 23.856601171045206 90-91 23.112922233606508 26.581590752331437 26.487549906005793 23.817937108056267 92-93 23.389113456939278 26.119958351421307 26.637348218394852 23.853579973244564 94-95 23.078639539785637 25.91298605851915 26.81160907147574 24.196765330219474 96-97 23.45427456247115 25.535876666526768 26.72794169079895 24.28190708020313 98-99 23.207804727926803 25.766653183173066 26.26709673959799 24.758445349302143 100-101 24.308068136647687 24.685425041774884 26.508348090796268 24.49815873078116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 178.0 1 135.5 2 132.5 3 187.0 4 174.0 5 162.5 6 265.5 7 549.0 8 897.0 9 1105.5 10 1018.5 11 885.0 12 932.0 13 1024.0 14 1174.0 15 1341.5 16 1462.5 17 1692.0 18 1980.5 19 2270.5 20 2718.5 21 3257.5 22 3987.0 23 4938.0 24 6231.0 25 8046.5 26 10764.0 27 14150.5 28 16950.5 29 19659.5 30 23168.0 31 28246.5 32 34650.5 33 37740.5 34 38520.0 35 41051.5 36 45000.5 37 49371.0 38 52964.0 39 55390.0 40 58301.5 41 62455.0 42 65610.5 43 67595.0 44 69574.5 45 72954.0 46 75427.0 47 77217.5 48 78547.5 49 77880.5 50 76601.0 51 75891.5 52 75835.0 53 76262.5 54 76630.0 55 73808.5 56 68933.0 57 63835.0 58 59862.0 59 54155.5 60 45479.5 61 38413.0 62 32754.0 63 25985.5 64 19248.0 65 14489.0 66 10802.5 67 8173.5 68 6449.0 69 5129.5 70 4106.0 71 3072.5 72 2309.0 73 1847.0 74 1407.5 75 1056.5 76 815.5 77 568.0 78 386.0 79 249.0 80 145.5 81 97.0 82 63.0 83 40.0 84 21.0 85 17.5 86 14.0 87 7.0 88 4.5 89 5.5 90 4.0 91 3.5 92 3.5 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38096241615306753 2 0.028860082682505055 3 0.03482791884302306 4 0.04349993076377579 5 0.06350150664551193 6 0.054596376124738964 7 0.036319877883152565 8 0.0380449555233023 9 0.03496779000303521 10-11 0.03692598624320517 12-13 0.029069889422523264 14-15 0.026389025522290565 16-17 0.021610094221875757 18-19 0.02652889668230271 20-21 0.034944478143033184 22-23 0.03375557328292999 24-25 0.04317356472374746 26-27 0.036879362523201134 28-29 0.031960560062774174 30-31 0.03496779000303521 32-33 0.029349631742547545 34-35 0.031167956822705376 36-37 0.03328933608288952 38-39 0.01456991250126467 40-41 0.012192102781058275 42-43 0.012262038361064346 44-45 0.010863326760942937 46-47 0.020188070761752323 48-49 0.017507206861519624 50-51 0.011143069080967218 52-53 0.009021689820783083 54-55 0.008019279840696074 56-57 0.008672011920752731 58-59 0.003823145040331849 60-61 0.006876998700596923 62-63 0.0114228114009915 64-65 0.007086805440615135 66-67 0.00342684342029745 68-69 0.005664781980491702 70-71 0.012704963701102792 72-73 0.010467025140908538 74-75 0.011469435120995547 76-77 0.004825555020418858 78-79 0.009348055860811411 80-81 0.011376187680987453 82-83 0.013940492281210035 84-85 0.008252398440716308 86-87 0.002098067400182112 88-89 0.015688881781361796 90-91 0.025643046002225816 92-93 0.010257218400890326 94-95 0.020467813081776608 96-97 0.018696111721622822 98-99 0.010886638620944961 100-101 0.12562761355090446 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2144831.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.39467946964842 #Duplication Level Percentage of deduplicated Percentage of total 1 76.62212601491227 41.67835984864163 2 12.847944908335423 13.977196902652139 3 4.406855136610052 7.1912841787523245 4 2.025477287995198 4.407007514142062 5 1.1125354126696927 3.025800358540049 6 0.6579711377055413 2.147407748146369 7 0.44603827480115454 1.6983476292302635 8 0.30901748289677966 1.3447125546150316 9 0.22067935442782022 1.08034044747032 >10 1.218122140607841 12.060616205730328 >50 0.08487008212502817 3.1532982831579717 >100 0.04214789586127124 4.113693729928928 >500 0.0033663884864603578 1.2598686763272626 >1k 0.0028484825654663945 2.862065922665364 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4979 0.2321395019001497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3894 0.1815527656957588 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2923 0.1362811335718292 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2856 0.13315734433155807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2698 0.12579079657091863 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2632 0.12271363105065154 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2590 0.12075543481048158 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2260 0.10536960720914608 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2199 0.10252556028889923 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2174 0.10135996728879804 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2154 0.10042749288871711 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.662372000404694E-5 2 0.0 0.0 0.0 0.0 4.662372000404694E-5 3 0.0 0.0 0.0 0.0 4.662372000404694E-5 4 0.0 0.0 0.0 0.0 4.662372000404694E-5 5 0.0 0.0 0.0 0.0 4.662372000404694E-5 6 0.0 0.0 0.0 0.0 4.662372000404694E-5 7 0.0 0.0 0.0 0.0 4.662372000404694E-5 8 0.0 0.0 0.0 0.0 9.324744000809388E-5 9 0.0 0.0 0.0 0.0 9.324744000809388E-5 10-11 0.0 0.0 0.0 0.0 9.324744000809388E-5 12-13 0.0 0.0 0.0 0.0 3.030541800263051E-4 14-15 0.0 0.0 0.0 0.0 3.7298976003237547E-4 16-17 0.0 0.0 0.0 0.0 4.1961348003642245E-4 18-19 0.0 0.0 0.0 0.0 4.1961348003642245E-4 20-21 0.0 0.0 0.0 4.662372000404694E-5 4.6623720004046937E-4 22-23 0.0 0.0 0.0 2.0980674001821122E-4 4.6623720004046937E-4 24-25 0.0 0.0 0.0 5.594846400485633E-4 5.128609200445163E-4 26-27 0.0 0.0 0.0 0.001188904860103197 5.594846400485633E-4 28-29 0.0 0.0 0.0 0.0035434027203075672 5.594846400485633E-4 30-31 0.0 0.0 0.0 0.012308662081068391 5.594846400485633E-4 32-33 0.0 0.0 0.0 0.028860082682505055 6.061083600526102E-4 34-35 0.0 0.0 0.0 0.05515586076478753 6.527320800566571E-4 36-37 0.0 0.0 0.0 0.0996348896486483 6.527320800566571E-4 38-39 0.0 0.0 0.0 0.16702947691449815 6.527320800566571E-4 40-41 0.0 0.0 0.0 0.23864351084071425 6.527320800566571E-4 42-43 0.0 0.0 0.0 0.314919916767335 6.527320800566571E-4 44-45 0.0 0.0 0.0 0.4103586716156191 6.760439400586806E-4 46-47 0.0 0.0 0.0 0.5246800330655422 7.226676600627275E-4 48-49 0.0 0.0 0.0 0.646601060876125 7.45979520064751E-4 50-51 0.0 0.0 0.0 0.7791056731276264 7.45979520064751E-4 52-53 0.0 0.0 0.0 0.9106778109790469 7.92603240068798E-4 54-55 0.0 0.0 0.0 1.0529967162914002 7.92603240068798E-4 56-57 0.0 0.0 0.0 1.2204691185459366 8.392269600728449E-4 58-59 0.0 0.0 0.0 1.4056585344020112 9.091625400789152E-4 60-61 0.0 0.0 0.0 1.6032032360591582 9.790981200849857E-4 62-63 0.0 0.0 0.0 1.7960389419958962 0.0010257218400890327 64-65 0.0 0.0 0.0 2.001323181173715 0.0010257218400890327 66-67 0.0 0.0 0.0 2.2353276318740267 0.001049033700091056 68-69 0.0 0.0 0.0 2.5017122561171483 0.0010723455600930795 70-71 0.0 2.331186000202347E-5 0.0 2.7827833521615455 0.0010723455600930795 72-73 0.0 4.662372000404694E-5 0.0 3.07499751728691 0.00114228114009915 74-75 0.0 4.662372000404694E-5 0.0 3.359588704191612 0.0011655930001011736 76-77 0.0 4.662372000404694E-5 2.331186000202347E-5 3.6737626414388824 0.001235528580107244 78-79 0.0 4.662372000404694E-5 4.662372000404694E-5 4.021948582429105 0.0012588404401092674 80-81 0.0 4.662372000404694E-5 4.662372000404694E-5 4.390299282321077 0.0012821523001112908 82-83 0.0 4.662372000404694E-5 4.662372000404694E-5 4.770072793614043 0.0013520878801173612 84-85 0.0 4.662372000404694E-5 4.662372000404694E-5 5.1560006359475405 0.0013520878801173612 86-87 2.331186000202347E-5 4.662372000404694E-5 4.662372000404694E-5 5.551346469721857 0.0014220234601234317 88-89 4.662372000404694E-5 4.662372000404694E-5 4.662372000404694E-5 5.960959161817411 0.001491959040129502 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3125 0.0 59.68899 1 GTATCAA 5805 0.0 46.267254 1 ATCAACG 7755 0.0 34.320957 3 TATCAAC 7750 0.0 34.281773 2 TCAACGC 7875 0.0 33.858322 4 CAACGCA 8075 0.0 32.960102 5 AACGCAG 8490 0.0 31.340935 6 TGGTATC 1230 0.0 28.980732 2 ACGCAGA 9700 0.0 27.326414 7 CGCAGAG 9870 0.0 26.470716 8 TAAGGTG 1465 0.0 24.331379 5 AGGTAAG 1295 0.0 24.22297 2 GTACATG 7705 0.0 23.775307 1 TACATGG 7485 0.0 23.748323 2 GCAGAGT 11015 0.0 23.157934 9 ACATGGG 7520 0.0 22.81616 3 GTGGTAT 1815 0.0 22.604212 1 AAGGTAA 1330 0.0 22.597294 1 GTAAGGT 1530 0.0 22.366306 4 GGTAAGG 1450 0.0 22.289179 3 >>END_MODULE