FastQCFastQC Report
Thu 26 May 2016
SRR938664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938664_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144831
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51900.2419771068210036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44880.20924725537816266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29970.13973128885212865No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28510.1329242257315378No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27810.12966056533125453No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27550.12844834861114932No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26860.1252313119308701No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25400.11842424881027921No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22580.10527635976913799No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31350.060.6205521
GTATCAA57650.043.843961
ATCAACG77750.032.5606163
TATCAAC78950.032.2476962
TCAACGC79800.031.7836764
CAACGCA80800.031.2727495
AACGCAG83400.030.354776
TGGTATC12600.030.1582052
ACGCAGA96150.026.2801677
CGCAGAG97000.025.8540138
TACATGG73250.024.3169822
GTGGTAT18350.024.0793251
GTACATG76300.023.7868121
TAAGGTG14400.023.4186385
ACATGGG75600.022.7432523
GTAAGGT14400.022.429124
GCAGAGT111250.022.2435199
AGAGTAC99850.021.1203310-11
GAGTACT67450.020.702912-13
AAGGTAA13800.020.6571121
Produced by FastQC (version 0.11.5)