##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938664_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2144831 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77041034934687 31.0 31.0 34.0 30.0 34.0 2 32.03325856442769 31.0 31.0 34.0 30.0 34.0 3 32.089104456248535 33.0 31.0 34.0 30.0 34.0 4 35.75288682418335 37.0 35.0 37.0 35.0 37.0 5 35.63617553084602 37.0 35.0 37.0 33.0 37.0 6 35.67141746832268 37.0 35.0 37.0 33.0 37.0 7 35.59169883314816 37.0 35.0 37.0 33.0 37.0 8 35.55728213551557 37.0 35.0 37.0 33.0 37.0 9 37.10556216317276 39.0 37.0 39.0 33.0 39.0 10-11 37.03008488780701 39.0 37.0 39.0 33.0 39.0 12-13 36.90744072609917 39.0 37.0 39.0 33.0 39.0 14-15 37.91320574907767 40.0 37.0 41.0 33.0 41.0 16-17 37.99558193629242 40.0 37.0 41.0 33.0 41.0 18-19 38.13583680952019 40.0 37.0 41.0 33.0 41.0 20-21 38.087292891607774 40.0 37.0 41.0 33.0 41.0 22-23 37.95914130297446 40.0 37.0 41.0 33.0 41.0 24-25 37.82644180357333 40.0 37.0 41.0 32.0 41.0 26-27 37.60191735386145 40.0 37.0 41.0 32.0 41.0 28-29 37.4562294185416 39.0 36.5 41.0 31.5 41.0 30-31 37.18618809593856 39.0 36.0 41.0 31.0 41.0 32-33 37.07297194977133 39.0 36.0 41.0 31.0 41.0 34-35 36.93103116282822 39.0 36.0 41.0 30.5 41.0 36-37 36.78727997683734 39.0 35.0 41.0 30.0 41.0 38-39 36.559214455591146 39.0 35.0 40.0 30.0 41.0 40-41 36.319555247010136 39.0 35.0 40.0 30.0 41.0 42-43 36.041450818269595 38.0 35.0 40.0 29.5 41.0 44-45 35.73356408966487 38.0 35.0 40.0 28.5 41.0 46-47 35.30633858798199 38.0 34.0 40.0 27.0 41.0 48-49 34.87290513798057 38.0 34.0 40.0 26.0 41.0 50-51 34.45667444194904 38.0 33.0 40.0 24.5 41.0 52-53 33.83312205017552 37.0 33.0 40.0 23.5 41.0 54-55 33.37718566171414 37.0 32.0 39.5 22.5 41.0 56-57 32.9608286154014 36.0 31.0 39.0 21.0 40.0 58-59 32.39335080479534 36.0 31.0 39.0 18.5 40.0 60-61 31.87511440295296 35.0 30.0 39.0 17.0 40.0 62-63 31.337511673413896 35.0 29.5 38.0 12.5 40.0 64-65 30.660429656229326 34.5 28.5 38.0 8.0 40.0 66-67 29.983678434338184 34.0 28.0 37.5 7.0 39.5 68-69 29.22994445716236 34.0 26.5 36.5 2.0 39.0 70-71 28.4285759577328 33.0 26.0 36.0 2.0 39.0 72-73 27.82581704572528 32.5 25.5 35.5 2.0 38.0 74-75 27.10624100453602 32.0 24.5 35.0 2.0 37.5 76-77 25.985912176763577 30.5 23.0 34.0 2.0 36.0 78-79 26.468832742533095 31.5 24.0 34.5 2.0 36.0 80-81 26.075519702950956 31.5 22.5 35.0 2.0 36.0 82-83 25.37345692970682 31.0 20.5 34.0 2.0 36.0 84-85 24.573083147343546 31.0 18.0 34.0 2.0 35.0 86-87 23.648439667274484 30.0 9.5 34.0 2.0 35.0 88-89 22.633302810337973 29.0 4.0 34.0 2.0 35.0 90-91 21.74001867746223 29.0 2.0 34.0 2.0 35.0 92-93 20.63027133606331 27.5 2.0 33.0 2.0 35.0 94-95 19.430834177611196 25.5 2.0 33.0 2.0 34.0 96-97 18.01681274655206 24.0 2.0 32.0 2.0 34.0 98-99 16.23860551250891 10.0 2.0 32.0 2.0 34.0 100-101 12.137721573401354 2.0 2.0 27.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2939399618491514 1101 2 0.25919706185702296 1101 3 0.2905362029931098 1101 4 0.20162557760647815 1101 5 0.19336415310930732 1101 6 0.23901801080566543 1101 7 0.29425582337265155 1101 8 0.1890733252350003 1101 9 0.3249184006532104 1101 10-11 0.39324760776977286 1101 12-13 0.4640911097387388 1101 14-15 0.5225032006370469 1101 16-17 0.4461566867663649 1101 18-19 0.44822694688804887 1101 20-21 0.40128978938832205 1101 22-23 0.4024222116445131 1101 24-25 0.4116652528953253 1101 26-27 0.5551241636782791 1101 28-29 0.48664105525825363 1101 30-31 0.5333458671469771 1101 32-33 0.5071244362892884 1101 34-35 0.3715243099867749 1101 36-37 0.38243621527515614 1101 38-39 0.4691992173319264 1101 40-41 0.4737983889017485 1101 42-43 0.4386947941122159 1101 44-45 0.6048019616450944 1101 46-47 0.5865620095786639 1101 48-49 0.7049663453158743 1101 50-51 0.8883497577646295 1101 52-53 0.7457449731454062 1101 54-55 0.8361408622402706 1101 56-57 1.1205755748835742 1101 58-59 1.2148925624656925 1101 60-61 1.1332510315526 1101 62-63 1.3288243617134547 1101 64-65 1.2813470997579053 1101 66-67 1.5158434985683265 1101 68-69 1.8506536285105675 1101 70-71 1.9059340782803247 1101 72-73 2.0400330886272577 1101 74-75 2.0711345671368875 1101 76-77 1.9702842973062467 1101 78-79 2.2062552475430053 1101 80-81 2.1577171509405275 1101 82-83 2.3334398340103135 1101 84-85 2.1862864274419316 1101 86-87 2.442377729627818 1101 88-89 2.4978577061370686 1101 90-91 2.6236801893478763 1101 92-93 3.019618135388228 1101 94-95 2.998827941344288 1101 96-97 3.1290920450939588 1101 98-99 3.031204441802725 1101 100-101 2.519227348800774 1102 1 0.24148537820953564 1102 2 0.23808064196133216 1102 3 0.2477608170853074 1102 4 0.1895635523343273 1102 5 0.23574138017360724 1102 6 0.23311650917803206 1102 7 0.21410863586171303 1102 8 0.2414255251719979 1102 9 0.3675756515195374 1102 10-11 0.3742361792983502 1102 12-13 0.33513227323763317 1102 14-15 0.35346420732261663 1102 16-17 0.38271991964116836 1102 18-19 0.36093521459080335 1102 20-21 0.34923117690626526 1102 22-23 0.5134477113119758 1102 24-25 0.3280466453136768 1102 26-27 0.4031990000393719 1102 28-29 0.4535562430492135 1102 30-31 0.37076536030389917 1102 32-33 0.4041666848804226 1102 34-35 0.4548462673531546 1102 36-37 0.3517464816478011 1102 38-39 0.5256896440182004 1102 40-41 0.5961364126337614 1102 42-43 0.5985459439599552 1102 44-45 0.6651527789645684 1102 46-47 0.6618017799591485 1102 48-49 0.7188677769375644 1102 50-51 0.9482188466371824 1102 52-53 0.6949092771112504 1102 54-55 0.7929625045798616 1102 56-57 0.9893432951397969 1102 58-59 1.0586213419119517 1102 60-61 1.1370002877393723 1102 62-63 1.227594007133316 1102 64-65 1.313520521998793 1102 66-67 1.3213030876333924 1102 68-69 1.408059820536888 1102 70-71 1.544907703538474 1102 72-73 1.7149538943957445 1102 74-75 1.7276849364182745 1102 76-77 1.7485544502692498 1102 78-79 1.9184699732946981 1102 80-81 2.2782617097280244 1102 82-83 2.3792705421205014 1102 84-85 2.318458099534059 1102 86-87 1.9914208357918426 1102 88-89 1.9105970389803595 1102 90-91 2.154680136843414 1102 92-93 2.4523879208199872 1102 94-95 2.0857941459716827 1102 96-97 2.002302517984152 1102 98-99 2.0929702543967963 1102 100-101 1.9201667411491226 1103 1 0.1810597017786506 1103 2 0.16476430358683558 1103 3 0.2267718996326593 1103 4 0.1771449088931476 1103 5 0.09977124888539635 1103 6 0.18130065657848604 1103 7 0.1574608270077107 1103 8 0.19589035592456128 1103 9 0.21025506583828957 1103 10-11 0.25985186682038375 1103 12-13 0.315984786800648 1103 14-15 0.38654978109592975 1103 16-17 0.3573022821410561 1103 18-19 0.3608806186438045 1103 20-21 0.39894315104412215 1103 22-23 0.3587922246342501 1103 24-25 0.3160045849400177 1103 26-27 0.38153236203199725 1103 28-29 0.3391644075076812 1103 30-31 0.44168914952055616 1103 32-33 0.5353530075209747 1103 34-35 0.5526123473641533 1103 36-37 0.4878077494774615 1103 38-39 0.38338982199238103 1103 40-41 0.5274469732954472 1103 42-43 0.4305411034059077 1103 44-45 0.5595775440300415 1103 46-47 0.8850323554090238 1103 48-49 0.9770988682546644 1103 50-51 0.8363205842499184 1103 52-53 0.9965546785849071 1103 54-55 1.1279688239532533 1103 56-57 1.290445746368846 1103 58-59 1.1767168891180972 1103 60-61 1.2313294024252492 1103 62-63 1.3419278076130787 1103 64-65 1.5246017796830635 1103 66-67 1.5290649547288062 1103 68-69 1.2984769421697813 1103 70-71 1.5255678416189333 1103 72-73 1.87093924376682 1103 74-75 1.7638296027811542 1103 76-77 1.7627094167808686 1103 78-79 1.9799789708787188 1103 80-81 2.0741940967316133 1103 82-83 2.1204629691897736 1103 84-85 2.1726250169695085 1103 86-87 2.0930430232407957 1103 88-89 1.9646197285400682 1103 90-91 2.2844340681814863 1103 92-93 2.457962081031148 1103 94-95 2.172366552234312 1103 96-97 2.1681301839429317 1103 98-99 2.209768825444609 1103 100-101 2.0166274621595353 1104 1 0.22447373255522862 1104 2 0.24155447582781875 1104 3 0.28333337766570565 1104 4 0.18222281763362247 1104 5 0.21842448038477613 1104 6 0.14916430444868212 1104 7 0.19358656766041094 1104 8 0.05510655550924071 1104 9 0.1462120436665586 1104 10-11 0.17437051202536225 1104 12-13 0.17553192616786362 1104 14-15 0.2651652673489622 1104 16-17 0.3285153575553963 1104 18-19 0.3392642618215689 1104 20-21 0.3766099638346958 1104 22-23 0.3005052496255516 1104 24-25 0.3311911810555941 1104 26-27 0.4726448383383115 1104 28-29 0.39487949881453943 1104 30-31 0.36183510878285574 1104 32-33 0.5681278799712715 1104 34-35 0.5692972280673629 1104 36-37 0.6165607156899071 1104 38-39 0.7471703356487964 1104 40-41 0.7507037253519897 1104 42-43 0.7091718194748111 1104 44-45 0.598809138077236 1104 46-47 0.7083514673872742 1104 48-49 0.8062143658271523 1104 50-51 0.8835807609569457 1104 52-53 0.881924999789959 1104 54-55 0.9755964714200616 1104 56-57 1.1439121759443296 1104 58-59 1.158559141155088 1104 60-61 1.1247854180996164 1104 62-63 1.09743029892282 1104 64-65 1.363758871117529 1104 66-67 1.3865558631461603 1104 68-69 1.4297448860828972 1104 70-71 1.7664992066014698 1104 72-73 1.9290959421073843 1104 74-75 1.8597543921877886 1104 76-77 1.8719973405659296 1104 78-79 2.002263278611551 1104 80-81 2.0784185183587702 1104 82-83 2.2119850040578193 1104 84-85 2.4282764106005637 1104 86-87 2.3181352768213443 1104 88-89 2.031465654255612 1104 90-91 2.562511159862918 1104 92-93 2.7302564733717887 1104 94-95 2.8854230322122483 1104 96-97 2.8111245715499784 1104 98-99 2.728151316095765 1104 100-101 2.4287933066219534 1105 1 0.11763727797691459 1105 2 0.17326612945266362 1105 3 0.11356500432768968 1105 4 0.1270446770945597 1105 5 0.07260421329608135 1105 6 0.1369548146916415 1105 7 0.14476814694199192 1105 8 0.16234745309918708 1105 9 0.1262419372804615 1105 10-11 0.2380722401888562 1105 12-13 0.19589524103749056 1105 14-15 0.2294365372414049 1105 16-17 0.13652557609139393 1105 18-19 0.3029361940627098 1105 20-21 0.22973560493548462 1105 22-23 0.28425230666764634 1105 24-25 0.17811194344043457 1105 26-27 0.35103497930721517 1105 28-29 0.43844992953658135 1105 30-31 0.43203234801968904 1105 32-33 0.4360811443343664 1105 34-35 0.6298850381159227 1105 36-37 0.5677955368655248 1105 38-39 0.7166866647911689 1105 40-41 0.6536803868410033 1105 42-43 0.6514180412541677 1105 44-45 0.5770962144622658 1105 46-47 0.7637474600799479 1105 48-49 0.9902671974166921 1105 50-51 1.03348697707019 1105 52-53 0.8334729223791726 1105 54-55 0.7917238807378908 1105 56-57 0.9118517634906098 1105 58-59 1.1966891295297941 1105 60-61 1.2506751438794375 1105 62-63 1.2428554036970034 1105 64-65 1.382665116538238 1105 66-67 1.5543000675950331 1105 68-69 1.66274802198161 1105 70-71 1.5453607092928685 1105 72-73 1.7863104629791842 1105 74-75 2.0571323510205666 1105 76-77 1.8994012853506668 1105 78-79 1.9239937867052959 1105 80-81 1.9742744428627432 1105 82-83 2.1881306816952453 1105 84-85 2.2753144028438577 1105 86-87 2.163627300306395 1105 88-89 2.377265406119079 1105 90-91 2.357669566709145 1105 92-93 2.399815786795582 1105 94-95 2.3664679410171914 1105 96-97 2.682502901610647 1105 98-99 2.8173988957876475 1105 100-101 2.2498590237267653 1106 1 0.011776089375970855 1106 2 0.015185555359785496 1106 3 0.007736345027758773 1106 4 0.06334047687178668 1106 5 0.04283300271321622 1106 6 -0.04255172566933396 1106 7 0.07645858628470137 1106 8 0.008222639156933553 1106 9 -0.1075155912812491 1106 10-11 0.10576768707245066 1106 12-13 0.0015311888723275047 1106 14-15 0.02840249976370046 1106 16-17 -0.0683602263473162 1106 18-19 0.04190186086408687 1106 20-21 0.05224588123739693 1106 22-23 0.2321029084597157 1106 24-25 0.08112780393901886 1106 26-27 -0.11857427478605587 1106 28-29 0.23716945796029165 1106 30-31 0.3391178212224446 1106 32-33 0.11930982532049939 1106 34-35 0.07738323914699663 1106 36-37 0.07392533863393425 1106 38-39 0.2278077427924856 1106 40-41 0.20624379433457563 1106 42-43 0.2590599626515413 1106 44-45 0.2647804239509597 1106 46-47 -0.0896755095495152 1106 48-49 0.1959568678850161 1106 50-51 0.38733036327185033 1106 52-53 0.2386588815499664 1106 54-55 0.10981657938841494 1106 56-57 0.339240449208269 1106 58-59 0.463965638445778 1106 60-61 0.6616764549088394 1106 62-63 0.5015101692396051 1106 64-65 0.6321076826072236 1106 66-67 0.3305724068460698 1106 68-69 0.5122891616900311 1106 70-71 0.4464962776191861 1106 72-73 0.5149327977944118 1106 74-75 0.6329749092036607 1106 76-77 0.6575700828508388 1106 78-79 0.7574427797755732 1106 80-81 0.8811667117322024 1106 82-83 1.2499905691241544 1106 84-85 1.2567653082427341 1106 86-87 1.2421776763648253 1106 88-89 1.142012515447103 1106 90-91 1.3241712868191513 1106 92-93 1.2368370885590636 1106 94-95 1.1148557691441923 1106 96-97 1.2202842061341919 1106 98-99 1.3378780622489508 1106 100-101 1.144206318373234 1107 1 0.16875245241279657 1107 2 0.10235455030205998 1107 3 0.08819282207621626 1107 4 0.11970343542074602 1107 5 0.09166524456033898 1107 6 0.055165008463013976 1107 7 0.11988848508127603 1107 8 0.10809847098096981 1107 9 0.1952038776820899 1107 10-11 0.20165223121672682 1107 12-13 0.21163157037879898 1107 14-15 0.3397843868655741 1107 16-17 0.21099732809734206 1107 18-19 0.16950604129053204 1107 20-21 0.18262608634482547 1107 22-23 0.2211989073873113 1107 24-25 0.17809424526344486 1107 26-27 0.21073812314701712 1107 28-29 0.31368308172387316 1107 30-31 0.29453890202373145 1107 32-33 0.3211296865535047 1107 34-35 0.48599516809814247 1107 36-37 0.5530472768707924 1107 38-39 0.45154715204942164 1107 40-41 0.6113554331685762 1107 42-43 0.4930868373806305 1107 44-45 0.4651485253520349 1107 46-47 0.627496293609191 1107 48-49 0.7401949850688467 1107 50-51 0.7117128247371554 1107 52-53 0.5208517561166275 1107 54-55 0.5480840566218035 1107 56-57 0.6975542864745776 1107 58-59 0.8999932939960118 1107 60-61 1.0612964161417544 1107 62-63 1.242106117775851 1107 64-65 1.2761501052663 1107 66-67 1.203899634510229 1107 68-69 1.3305216291729387 1107 70-71 1.4040385892216243 1107 72-73 1.6113399441670637 1107 74-75 1.3723419939716806 1107 76-77 1.3752669344194786 1107 78-79 1.6347724497074019 1107 80-81 1.8773287507125964 1107 82-83 1.9768933803738484 1107 84-85 2.270855314025507 1107 86-87 2.5084629344027363 1107 88-89 2.4563895072504387 1107 90-91 2.5185242277653934 1107 92-93 2.5231156734136775 1107 94-95 2.3767985656776602 1107 96-97 2.5057327526620305 1107 98-99 2.475675774775649 1107 100-101 1.8187788481457083 1108 1 0.04120626678983896 1108 2 0.04506986806327973 1108 3 0.03276257262709947 1108 4 0.061865442401902726 1108 5 -0.0476517862320307 1108 6 0.0640911702006619 1108 7 0.07232772747732241 1108 8 0.08278944306786684 1108 9 0.003765849678785571 1108 10-11 0.13190543127974763 1108 12-13 0.08492742715328916 1108 14-15 0.16874393022317236 1108 16-17 0.06679399955974219 1108 18-19 -0.03456152483551023 1108 20-21 0.09884515496396773 1108 22-23 0.13316668654898223 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-0.6966839512176861 2316 60-61 -0.5933550343711111 2316 62-63 -0.6232134373482658 2316 64-65 -0.5231309420628385 2316 66-67 -0.46453257615493015 2316 68-69 -0.09989013355913912 2316 70-71 -0.3530111754738918 2316 72-73 -0.35803976366409884 2316 74-75 0.004226315568143235 2316 76-77 0.02781749059282035 2316 78-79 -0.047967878653366824 2316 80-81 -0.6223902535657224 2316 82-83 -0.15035676841456436 2316 84-85 0.08899790483353343 2316 86-87 0.2375920850990525 2316 88-89 0.5321083372800111 2316 90-91 0.6396729233624505 2316 92-93 0.5249141833906954 2316 94-95 1.069941622522581 2316 96-97 0.9380485949928214 2316 98-99 0.43274985188032744 2316 100-101 0.04890728006595957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 18.0 4 38.0 5 79.0 6 142.0 7 458.0 8 1083.0 9 2058.0 10 3219.0 11 5063.0 12 7542.0 13 10321.0 14 13634.0 15 18184.0 16 22376.0 17 27751.0 18 28129.0 19 27079.0 20 28223.0 21 30435.0 22 33516.0 23 37483.0 24 41814.0 25 47562.0 26 54782.0 27 63885.0 28 74990.0 29 87587.0 30 101341.0 31 116893.0 32 136845.0 33 162263.0 34 195499.0 35 231910.0 36 257825.0 37 211369.0 38 62378.0 39 1051.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.07863237659449 19.164747266399388 15.304852371343443 23.451767985662674 2 15.964813705215693 20.281499684088065 39.274125296968876 24.479561313727363 3 19.36522693512396 23.626700509791597 30.065861680214173 26.942210874870266 4 13.571045923897968 16.983995475634213 36.03178991724756 33.41316868322026 5 13.976232115690449 37.146585715304774 34.13221672423929 14.744965444765477 6 32.99103752230362 36.02022723468656 16.726679164931877 14.262056078077947 7 29.084715765484553 32.442043219255964 20.066522723701773 18.406718291557702 8 26.706393184358113 35.124725444568824 19.187852096505505 18.981029274567554 9 26.22141552675344 16.739625153916844 19.672495730424878 37.36646358890484 10-11 25.262270080952764 26.34181900578647 27.099058154232196 21.296852759028567 12-13 26.199103798854082 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22.731628920025113 38-39 22.468921653657127 26.290345895233564 28.257770752315135 22.98296169879417 40-41 22.963129792028916 26.135783420226925 28.274456920875306 22.62662986686885 42-43 22.74697972761422 26.12915326065392 28.073373834431653 23.050493177300204 44-45 22.661153533773028 26.406273166301293 28.263447061708728 22.66912623821695 46-47 23.14445580510148 26.697209894122096 27.44990393173535 22.708430369041075 48-49 22.794872882758597 26.788427619705235 27.41871037857994 22.997989118956223 50-51 22.92336785508975 26.8997650630749 27.448689430542544 22.728177651292807 52-53 23.378788352089277 26.445789901395493 27.433303602941212 22.742118143574018 54-55 22.803871260719376 26.817870498887793 27.46335259048382 22.914905649909016 56-57 22.821588274320913 26.980260915661887 27.258930890126077 22.939219919891123 58-59 22.670713916387818 26.50127212820031 27.425144451940504 23.40286950347137 60-61 22.392323684243653 26.741547469241166 27.67868424132251 23.18744460519267 62-63 22.562733380858447 26.707232411318188 27.799532923573 22.93050128425037 64-65 22.328218782993606 26.726379288600466 27.573300149639003 23.372101778766925 66-67 22.214612148909605 26.88253735281968 27.45708493681049 23.445765561460227 68-69 22.862508648808934 26.38033591880657 27.56167929563727 23.195476136747228 70-71 22.624087980906637 26.479779973135386 27.221191059986598 23.674940985971375 72-73 22.59526025825459 26.615431633821274 27.189768472574528 23.599539635349608 74-75 22.643858653665486 26.994597709563127 26.852558546570805 23.50898509020058 76-77 22.635326512904747 26.536239918203346 27.371946787415887 23.456486781476023 78-79 22.67337146842805 26.816285292407656 27.167898076818176 23.342445162346124 80-81 22.91150356450743 26.748387340421242 26.924089197890833 23.416019897180497 82-83 22.84992209085577 26.833569096692194 26.967683790515316 23.34882502193672 84-85 22.69112955925342 26.94544970474761 26.95857514172791 23.404845594271062 86-87 22.832531177985235 26.81077098999064 26.77503243396352 23.581665398060604 88-89 22.66197196888706 26.882257856213382 26.466141155177258 23.9896290197223 90-91 22.511337296248627 26.897407522327825 26.763828727273832 23.82742645414972 92-93 22.739462761944814 26.454689480059418 26.8743513447736 23.93149641322217 94-95 22.403376307243214 26.326393928465606 27.04824651481459 24.22198324947659 96-97 22.689951490339215 25.933354640378344 27.04188046561255 24.334813403669894 98-99 22.335605928858733 26.21451293831542 26.6923594446369 24.75752168818895 100-101 22.545731490545055 25.799300130849563 27.017905152833826 24.63706322577156 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 194.0 1 141.0 2 121.5 3 166.5 4 160.0 5 168.5 6 297.0 7 625.5 8 1019.5 9 1192.5 10 1057.5 11 936.5 12 997.5 13 1135.5 14 1311.5 15 1482.0 16 1637.0 17 1839.0 18 2128.0 19 2460.5 20 2876.5 21 3394.0 22 4139.0 23 5124.5 24 6476.0 25 8400.5 26 11284.5 27 14691.5 28 17402.5 29 20203.5 30 23563.0 31 28721.0 32 35878.0 33 38694.5 34 38874.5 35 41321.0 36 45446.0 37 50018.5 38 53471.0 39 55904.5 40 59004.5 41 63325.0 42 66341.0 43 68171.0 44 70113.0 45 73728.0 46 75942.5 47 77463.0 48 79159.0 49 78400.5 50 77125.5 51 76368.5 52 76040.0 53 76679.0 54 78027.0 55 74857.5 56 68805.5 57 63143.5 58 58854.5 59 53034.0 60 43818.5 61 36941.5 62 31450.0 63 24004.5 64 16929.0 65 12438.0 66 9301.0 67 7159.0 68 5549.5 69 4394.5 70 3534.0 71 2534.0 72 1865.0 73 1534.5 74 1218.5 75 905.0 76 653.0 77 437.5 78 281.5 79 178.0 80 110.0 81 65.5 82 37.0 83 21.5 84 14.5 85 9.0 86 3.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023218612562015378 2 0.011469435120995547 3 0.0015385827601335491 4 0.0 5 1.8649488001618776E-4 6 0.0 7 0.0 8 0.0 9 1.8649488001618776E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017717013601537838 26-27 0.008485517040736542 28-29 2.5643046002225817E-4 30-31 0.0 32-33 3.96301620034399E-4 34-35 0.009977476080866046 36-37 0.001235528580107244 38-39 0.011702553721015781 40-41 0.011842424881027923 42-43 0.0053850396604674215 44-45 6.061083600526103E-4 46-47 9.324744000809388E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.1655930001011736E-4 66-67 6.061083600526103E-4 68-69 0.0012588404401092674 70-71 9.324744000809388E-5 72-73 4.4292534003844596E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.0980674001821122E-4 82-83 0.0011655930001011736 84-85 0.006550632660568594 86-87 0.004126199220358154 88-89 0.0 90-91 0.0013753997401193846 92-93 4.1961348003642245E-4 94-95 1.1655930001011736E-4 96-97 2.331186000202347E-5 98-99 0.0 100-101 6.993558000607041E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2144831.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.428038626152095 #Duplication Level Percentage of deduplicated Percentage of total 1 74.15724915377012 38.13761873889274 2 13.938741381221616 14.336842603068197 3 5.00349650023655 7.719600338399463 4 2.262055044621621 4.653322168371318 5 1.2489033378285972 3.211432454908969 6 0.7480437206180314 2.308225281479679 7 0.498365796862785 1.7940982815708661 8 0.35929458277045345 1.4782252544708865 9 0.26802707117686897 1.240569591240459 >10 1.363770309458805 12.82635790793324 >50 0.09598641926186288 3.376333627577409 >100 0.04961804843193973 4.634212240402955 >500 0.0032697297841029023 1.2050697824348902 >1k 0.0030880781294305142 2.8356671325267344 >5k 9.08258273361916E-5 0.2424245967222401 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5190 0.2419771068210036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4488 0.20924725537816266 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2997 0.13973128885212865 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2851 0.1329242257315378 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2781 0.12966056533125453 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2755 0.12844834861114932 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2686 0.1252313119308701 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2540 0.11842424881027921 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2258 0.10527635976913799 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.662372000404694E-5 2 4.662372000404694E-5 0.0 0.0 0.0 4.662372000404694E-5 3 4.662372000404694E-5 0.0 0.0 0.0 4.662372000404694E-5 4 4.662372000404694E-5 0.0 0.0 0.0 4.662372000404694E-5 5 4.662372000404694E-5 0.0 0.0 0.0 4.662372000404694E-5 6 4.662372000404694E-5 0.0 0.0 0.0 4.662372000404694E-5 7 9.324744000809388E-5 0.0 0.0 0.0 4.662372000404694E-5 8 9.324744000809388E-5 0.0 0.0 0.0 4.662372000404694E-5 9 9.324744000809388E-5 0.0 0.0 0.0 9.324744000809388E-5 10-11 9.324744000809388E-5 0.0 0.0 0.0 9.324744000809388E-5 12-13 9.324744000809388E-5 0.0 0.0 0.0 3.030541800263051E-4 14-15 9.324744000809388E-5 0.0 0.0 0.0 3.96301620034399E-4 16-17 9.324744000809388E-5 0.0 0.0 0.0 4.1961348003642245E-4 18-19 9.324744000809388E-5 0.0 0.0 0.0 4.6623720004046937E-4 20-21 9.324744000809388E-5 0.0 0.0 4.662372000404694E-5 5.827965000505867E-4 22-23 9.324744000809388E-5 0.0 0.0 2.0980674001821122E-4 6.294202200546337E-4 24-25 9.324744000809388E-5 0.0 0.0 5.594846400485633E-4 6.760439400586806E-4 26-27 9.324744000809388E-5 0.0 0.0 0.001188904860103197 7.226676600627275E-4 28-29 2.3311860002023468E-4 0.0 0.0 0.0035667145803095907 7.45979520064751E-4 30-31 2.5643046002225817E-4 0.0 0.0 0.012401909521076485 7.45979520064751E-4 32-33 2.7974232002428163E-4 0.0 0.0 0.029163136862531358 7.45979520064751E-4 34-35 2.7974232002428163E-4 0.0 0.0 0.055738657264838114 7.45979520064751E-4 36-37 2.7974232002428163E-4 0.0 0.0 0.10066061148873734 7.45979520064751E-4 38-39 2.7974232002428163E-4 0.0 0.0 0.16826500549460538 7.45979520064751E-4 40-41 2.7974232002428163E-4 0.0 0.0 0.24090476126091054 7.45979520064751E-4 42-43 2.7974232002428163E-4 0.0 0.0 0.3179271467075961 7.692913800667746E-4 44-45 2.7974232002428163E-4 0.0 0.0 0.41415850479594896 8.625388200748684E-4 46-47 2.7974232002428163E-4 0.0 0.0 0.5291092864659267 9.557862600829622E-4 48-49 2.7974232002428163E-4 0.0 0.0 0.6518462293765803 9.790981200849857E-4 50-51 2.7974232002428163E-4 0.0 0.0 0.784653895808108 0.0010257218400890327 52-53 2.7974232002428163E-4 0.0 0.0 0.9162027217995264 0.001188904860103197 54-55 2.7974232002428163E-4 0.0 0.0 1.0581253254918455 0.0012588404401092674 56-57 2.7974232002428163E-4 0.0 0.0 1.225644351466386 0.0013287760201153376 58-59 2.7974232002428163E-4 0.0 0.0 1.4110668859224806 0.0013520878801173612 60-61 2.7974232002428163E-4 0.0 0.0 1.6074226827195242 0.0013520878801173612 62-63 2.7974232002428163E-4 0.0 0.0 1.8003982598162747 0.001445335320125455 64-65 2.7974232002428163E-4 0.0 0.0 2.00610211247413 0.001445335320125455 66-67 2.7974232002428163E-4 0.0 0.0 2.2403863054944653 0.0014686471801274785 68-69 2.7974232002428163E-4 0.0 0.0 2.5092186750378005 0.001491959040129502 70-71 2.7974232002428163E-4 0.0 0.0 2.794159539842533 0.001491959040129502 72-73 2.7974232002428163E-4 0.0 0.0 3.0909661413882956 0.001585206480137596 74-75 2.7974232002428163E-4 0.0 0.0 3.3838097267337144 0.001585206480137596 76-77 2.7974232002428163E-4 0.0 0.0 3.7086371840019097 0.0017250776401497368 78-79 3.030541800263051E-4 0.0 0.0 4.070716993553338 0.0017483895001517602 80-81 3.729897600323755E-4 0.0 0.0 4.456714771466843 0.001841636940159854 82-83 3.729897600323755E-4 0.0 0.0 4.856746289101565 0.0019581962401699713 84-85 3.729897600323755E-4 0.0 0.0 5.267594509777227 0.0019581962401699713 86-87 3.729897600323755E-4 0.0 0.0 5.688886443733796 0.002098067400182112 88-89 3.729897600323755E-4 0.0 0.0 6.135192003472534 0.002144691120186159 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3135 0.0 60.620552 1 GTATCAA 5765 0.0 43.84396 1 ATCAACG 7775 0.0 32.560616 3 TATCAAC 7895 0.0 32.247696 2 TCAACGC 7980 0.0 31.783676 4 CAACGCA 8080 0.0 31.272749 5 AACGCAG 8340 0.0 30.35477 6 TGGTATC 1260 0.0 30.158205 2 ACGCAGA 9615 0.0 26.280167 7 CGCAGAG 9700 0.0 25.854013 8 TACATGG 7325 0.0 24.316982 2 GTGGTAT 1835 0.0 24.079325 1 GTACATG 7630 0.0 23.786812 1 TAAGGTG 1440 0.0 23.418638 5 ACATGGG 7560 0.0 22.743252 3 GTAAGGT 1440 0.0 22.42912 4 GCAGAGT 11125 0.0 22.243519 9 AGAGTAC 9985 0.0 21.12033 10-11 GAGTACT 6745 0.0 20.7029 12-13 AAGGTAA 1380 0.0 20.657112 1 >>END_MODULE