FastQCFastQC Report
Thu 26 May 2016
SRR938663_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938663_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3077770
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135270.4395065258287656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102350.33254596672265957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71020.23075148565357387No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA60000.19494634101963434No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA59540.19345175240515047No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA53120.1725924939160496No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA47940.15576212647468785No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA46900.15238305656368084No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC46450.15092095900603358No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG41770.13571514440650212No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT40850.13272596717753438No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38790.1260328094691936No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA38150.12395338183165085No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG37490.12180897208043485No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG36750.11940463387452604No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36030.11706527778229042No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33980.11040461113078624No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT33620.10923493308466845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73450.053.875271
GTATCAA133200.042.4556581
TATCAAC173800.032.5095142
ATCAACG174200.032.352963
TCAACGC177900.031.5998744
CAACGCA180700.031.0312635
AACGCAG187150.029.9652586
ACGCAGA211250.026.4978627
GTACATG161850.026.1832121
CGCAGAG213600.026.0946798
TAAGGTG21150.026.0850355
TACATGG160600.025.9419332
GGACCGA10550.025.2267556
TGGTATC33050.024.6075552
ACATGGG164600.024.32913
GCAGAGT227100.023.9773659
GTGGTAT36400.023.780881
ACCGAGT11050.023.6513338
AGGACCG12600.022.647725
GTGTAAG12850.022.5779571
Produced by FastQC (version 0.11.5)