##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938663_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3077770 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.246740984544005 31.0 31.0 34.0 28.0 34.0 2 31.26924201613506 31.0 31.0 34.0 28.0 34.0 3 31.309420457019204 31.0 31.0 34.0 28.0 34.0 4 34.98440104361275 37.0 35.0 37.0 32.0 37.0 5 34.983033170119924 37.0 35.0 37.0 32.0 37.0 6 35.00968883314867 37.0 35.0 37.0 32.0 37.0 7 34.927133931385384 37.0 35.0 37.0 32.0 37.0 8 34.88934358317873 37.0 35.0 37.0 32.0 37.0 9 36.40044642712093 39.0 37.0 39.0 32.0 39.0 10-11 36.245817426253424 39.0 35.0 39.0 32.0 39.0 12-13 36.12285957040325 39.0 35.0 39.0 31.0 39.0 14-15 37.102300041913466 39.0 36.0 41.0 31.0 41.0 16-17 36.956871533610375 39.0 36.0 41.0 31.0 41.0 18-19 37.128421064601966 39.0 36.0 41.0 31.0 41.0 20-21 37.117263473228995 39.0 36.0 41.0 31.0 41.0 22-23 37.042584728553464 39.0 36.0 41.0 31.0 41.0 24-25 36.9144614444874 39.0 36.0 41.0 30.5 41.0 26-27 36.717006306514136 39.0 36.0 41.0 30.0 41.0 28-29 36.51847782647826 39.0 36.0 41.0 30.0 41.0 30-31 36.249505811025514 39.0 35.0 40.0 29.5 41.0 32-33 35.95529506753266 38.0 35.0 40.0 29.0 41.0 34-35 35.710783456853505 38.0 35.0 40.0 27.5 41.0 36-37 35.43378225143529 38.0 34.5 40.0 27.0 41.0 38-39 35.26750796843169 38.0 34.0 40.0 26.0 41.0 40-41 35.14364718611202 38.0 34.0 40.0 25.5 41.0 42-43 34.82630216033037 38.0 34.0 40.0 24.5 41.0 44-45 34.452287370401294 38.0 33.0 40.0 24.0 41.0 46-47 34.02415596357103 38.0 33.0 40.0 22.5 41.0 48-49 33.6192169980213 38.0 33.0 40.0 20.5 41.0 50-51 32.89045217803799 37.0 31.5 39.5 18.5 40.5 52-53 32.890568983387325 37.0 32.0 39.5 18.0 40.0 54-55 33.003343654659055 37.0 32.0 40.0 17.5 41.0 56-57 32.69986987331737 37.0 31.5 40.0 14.0 41.0 58-59 32.34662125499956 36.0 31.0 39.5 11.0 41.0 60-61 31.90199738771903 36.0 31.0 39.0 8.0 41.0 62-63 31.363657290830698 35.0 30.0 39.0 7.0 40.0 64-65 30.87283942594801 35.0 29.5 38.5 2.0 40.0 66-67 30.373133307557097 35.0 29.0 38.0 2.0 40.0 68-69 29.832500804153657 34.0 28.5 37.5 2.0 40.0 70-71 29.226542756606243 34.0 27.5 37.0 2.0 39.0 72-73 28.634099364150018 34.0 26.0 36.0 2.0 39.0 74-75 28.01153611218512 33.5 26.0 36.0 2.0 38.5 76-77 27.306816461269037 33.0 25.0 35.0 2.0 37.5 78-79 26.56136878324241 32.0 23.5 35.0 2.0 37.0 80-81 25.917742066496196 32.0 21.0 35.0 2.0 36.5 82-83 25.214436426373638 31.0 19.0 35.0 2.0 36.0 84-85 24.54547237122982 31.0 16.5 34.0 2.0 35.5 86-87 23.824116324481686 30.5 8.5 34.0 2.0 35.0 88-89 23.016455420645467 30.0 3.5 34.0 2.0 35.0 90-91 22.135437508325833 29.0 2.0 34.0 2.0 35.0 92-93 21.228741751332947 29.0 2.0 34.0 2.0 35.0 94-95 20.235426298911225 27.5 2.0 34.0 2.0 35.0 96-97 19.005462883841222 25.5 2.0 33.0 2.0 35.0 98-99 17.48823904970157 22.0 2.0 32.5 2.0 34.0 100-101 13.857559694194173 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40269184164390026 1101 2 0.3275844796656848 1101 3 0.3747234737470997 1101 4 0.35723422543806294 1101 5 0.32747341356238024 1101 6 0.49765869974470434 1101 7 0.42209519477378876 1101 8 0.4676864991173062 1101 9 0.633737085683471 1101 10-11 0.5649609453785658 1101 12-13 0.5616889768894495 1101 14-15 0.7830545573645153 1101 16-17 0.6329190637420297 1101 18-19 0.6122000733722146 1101 20-21 0.5979199117599592 1101 22-23 0.6421068657149789 1101 24-25 0.6458884664591054 1101 26-27 0.6332655716897264 1101 28-29 0.6531589404840261 1101 30-31 0.622075314402089 1101 32-33 0.6973702884556872 1101 34-35 0.5427002007210859 1101 36-37 0.7507538126462805 1101 38-39 0.7491656681023002 1101 40-41 0.6395201307944163 1101 42-43 0.802558442091204 1101 44-45 0.7636468934683407 1101 46-47 0.681703901368266 1101 48-49 0.541747773109158 1101 50-51 0.6528070053482438 1101 52-53 0.6202399856887695 1101 54-55 0.7245429702733759 1101 56-57 0.7286120498750464 1101 58-59 0.7749001846927754 1101 60-61 0.786023107399263 1101 62-63 0.7852038443606091 1101 64-65 0.688299189261226 1101 66-67 0.6767487222828343 1101 68-69 0.6338097022360927 1101 70-71 0.5804986202069884 1101 72-73 0.7361914892082453 1101 74-75 0.5574002062077845 1101 76-77 0.5251530421944786 1101 78-79 0.6312812394491623 1101 80-81 0.7318708610047828 1101 82-83 0.6875017691030152 1101 84-85 0.7996765013621605 1101 86-87 0.7298071289689467 1101 88-89 0.5979567590695005 1101 90-91 0.42776582425509346 1101 92-93 0.44775591178574814 1101 94-95 0.34016149306456 1101 96-97 0.12389845531945909 1101 98-99 -0.027211138925178346 1101 100-101 0.23548902743771905 1102 1 0.34825569963493663 1102 2 0.25634068041557967 1102 3 0.20603457590642904 1102 4 0.15305293009946297 1102 5 0.2090295781133804 1102 6 0.28109661908404604 1102 7 0.21223152532844836 1102 8 0.36493805703597815 1102 9 0.5038471132763078 1102 10-11 0.5007771789954063 1102 12-13 0.5027093155547746 1102 14-15 0.5937931117417463 1102 16-17 0.6967357727631693 1102 18-19 0.6258619234118399 1102 20-21 0.49773617005786264 1102 22-23 0.5801637138432909 1102 24-25 0.44291837961409186 1102 26-27 0.44542527051023484 1102 28-29 0.37047445467724316 1102 30-31 0.48275974673322963 1102 32-33 0.28270352247712793 1102 34-35 0.27965956423172145 1102 36-37 0.4770052674333698 1102 38-39 0.2188900834622629 1102 40-41 0.4292213106961569 1102 42-43 0.5987411189817138 1102 44-45 0.5148966661234269 1102 46-47 0.42159267204269923 1102 48-49 0.4544067052006753 1102 50-51 0.45042838255746176 1102 52-53 0.2410934568599714 1102 54-55 0.2621752747295716 1102 56-57 0.18137109893718417 1102 58-59 0.28905741893282055 1102 60-61 0.3441388580605853 1102 62-63 0.40994935762241624 1102 64-65 0.3482888202763945 1102 66-67 0.08645393342492369 1102 68-69 0.21742620669822443 1102 70-71 0.4096726067187859 1102 72-73 0.45070548234279784 1102 74-75 0.5139819553253666 1102 76-77 0.6529041360210108 1102 78-79 0.6644568490346963 1102 80-81 0.6153385592223692 1102 82-83 0.8357593007204862 1102 84-85 0.9357923454589319 1102 86-87 1.0151029594264962 1102 88-89 0.7562517706446279 1102 90-91 0.6816976057959465 1102 92-93 0.6748826628557794 1102 94-95 0.8059466344180031 1102 96-97 1.0438122978407556 1102 98-99 1.2375743605975096 1102 100-101 1.0395495912413537 1103 1 0.45838908550603463 1103 2 0.4483571612600663 1103 3 0.4657530683632416 1103 4 0.32172972838337444 1103 5 0.38513515050286173 1103 6 0.462033828640493 1103 7 0.37556748692103525 1103 8 0.39280528291724437 1103 9 0.5067704176000802 1103 10-11 0.7244994192923428 1103 12-13 0.775848245448806 1103 14-15 0.8344519945382487 1103 16-17 0.911454202838236 1103 18-19 0.9478415743510382 1103 20-21 0.7305489380508519 1103 22-23 0.8192088975547733 1103 24-25 0.8483465644521502 1103 26-27 0.8465858587960966 1103 28-29 0.7151450866289579 1103 30-31 0.8420643809013413 1103 32-33 0.8874402708474136 1103 34-35 0.9113840930473529 1103 36-37 0.855828805393891 1103 38-39 0.8992345979342105 1103 40-41 0.8514520515677546 1103 42-43 0.8488086763499467 1103 44-45 0.8462396791834266 1103 46-47 0.5569831220974706 1103 48-49 0.6849069615142227 1103 50-51 0.8553944626575358 1103 52-53 0.9534528974314895 1103 54-55 0.9907081069250196 1103 56-57 0.9743922548475226 1103 58-59 0.8528500834159942 1103 60-61 0.9984189103954328 1103 62-63 0.9599733437443305 1103 64-65 1.0135189931313953 1103 66-67 1.0862593846567314 1103 68-69 1.1394881055627515 1103 70-71 1.0681866654379668 1103 72-73 1.1224489944018714 1103 74-75 1.037945249635147 1103 76-77 1.1870174878933746 1103 78-79 1.0291793716261388 1103 80-81 0.9228492110759099 1103 82-83 0.9004083773816127 1103 84-85 1.222482577342145 1103 86-87 1.1980633939105196 1103 88-89 1.2093232213189715 1103 90-91 1.3130492837482244 1103 92-93 1.1300659753700586 1103 94-95 1.3611981418282006 1103 96-97 1.574949218083031 1103 98-99 1.7108627735936963 1103 100-101 1.4307077816005354 1104 1 0.4207784858895316 1104 2 0.2749574987089147 1104 3 0.29714645111489446 1104 4 0.17228375519520256 1104 5 0.26992862139834983 1104 6 0.2583917038869714 1104 7 0.2985158545808133 1104 8 0.38813312131918565 1104 9 0.4714875023004552 1104 10-11 0.5518958201627058 1104 12-13 0.5427640162984275 1104 14-15 0.6484510069581262 1104 16-17 0.696705006400812 1104 18-19 0.6304014143487038 1104 20-21 0.6008891147948816 1104 22-23 0.5778632350236137 1104 24-25 0.6385616118198385 1104 26-27 0.6540295260923443 1104 28-29 0.6690615391960151 1104 30-31 0.814433545121922 1104 32-33 0.8527379306260627 1104 34-35 0.7475664190036682 1104 36-37 0.7353425554986899 1104 38-39 0.8442634062003478 1104 40-41 0.9102994696687219 1104 42-43 0.959117556648927 1104 44-45 0.7047643390746146 1104 46-47 0.8312003071710663 1104 48-49 0.7046185045363913 1104 50-51 0.7689553709953785 1104 52-53 0.9202342989287047 1104 54-55 1.0383548742281192 1104 56-57 0.9837104912632313 1104 58-59 0.9159605429202102 1104 60-61 1.017685503959303 1104 62-63 1.0206372221806959 1104 64-65 1.008363232619061 1104 66-67 1.0288131535491374 1104 68-69 1.075489093142881 1104 70-71 1.2277071352922881 1104 72-73 1.179507128567657 1104 74-75 1.0944190911962224 1104 76-77 1.015774695728485 1104 78-79 1.306049524580562 1104 80-81 1.147166295058419 1104 82-83 1.1613701450577487 1104 84-85 1.3399948060812896 1104 86-87 1.424060671655635 1104 88-89 1.2515115060856026 1104 90-91 1.3130287091622108 1104 92-93 1.3515083217907105 1104 94-95 1.372046909519458 1104 96-97 1.574629959475736 1104 98-99 1.6376092995652343 1104 100-101 1.4188558586266566 1105 1 0.35163673047932775 1105 2 0.3065446715891298 1105 3 0.2726095091780927 1105 4 0.2269784834387707 1105 5 0.2292783760397441 1105 6 0.3859278469817937 1105 7 0.2869059777706582 1105 8 0.3863628154167671 1105 9 0.4949042635668306 1105 10-11 0.5088061636647581 1105 12-13 0.560597713014765 1105 14-15 0.6048809040696739 1105 16-17 0.5533657268001093 1105 18-19 0.6596234744 1105 20-21 0.3840135479474185 1105 22-23 0.5583980387066987 1105 24-25 0.5914549814314114 1105 26-27 0.5271236873382961 1105 28-29 0.5390041186747752 1105 30-31 0.6186805014138486 1105 32-33 0.5073788421467995 1105 34-35 0.5392280703842189 1105 36-37 0.7187223778314262 1105 38-39 0.6659568310701545 1105 40-41 0.8183165997238007 1105 42-43 0.7455910597211641 1105 44-45 0.8243315228863537 1105 46-47 0.7492327533693626 1105 48-49 0.7361311388529614 1105 50-51 0.7242539156614569 1105 52-53 0.8098472509801695 1105 54-55 0.7578156258603386 1105 56-57 0.7191708464776312 1105 58-59 1.0286211071549118 1105 60-61 0.813919834944155 1105 62-63 0.9092594299877277 1105 64-65 0.9249577031910476 1105 66-67 1.0030147019811864 1105 68-69 1.0705976575697882 1105 70-71 1.1135383213624017 1105 72-73 1.1050889317882522 1105 74-75 1.31114612148615 1105 76-77 1.4222256750607016 1105 78-79 1.237781510093292 1105 80-81 1.4369030375850436 1105 82-83 1.3449970077710454 1105 84-85 1.4059629965540488 1105 86-87 1.5517976664481488 1105 88-89 1.5900183322804118 1105 90-91 1.3966430186444043 1105 92-93 1.605077403602941 1105 94-95 1.722943714752173 1105 96-97 1.7963568396073946 1105 98-99 2.150424166434913 1105 100-101 1.9566925585083332 1106 1 0.1839986767091979 1106 2 0.024321527856553615 1106 3 -0.06534036065857052 1106 4 -0.08736851128558243 1106 5 0.1586486389005728 1106 6 -0.056604233097459655 1106 7 0.20307168837032918 1106 8 0.2143480482133242 1106 9 0.13897987349150043 1106 10-11 0.02840881991835431 1106 12-13 0.08431249404086572 1106 14-15 0.3099543369261255 1106 16-17 0.13848218267128942 1106 18-19 -0.005399908925276975 1106 20-21 0.2815927978379875 1106 22-23 0.25063800000326353 1106 24-25 -0.01877700880057631 1106 26-27 0.011770115333781916 1106 28-29 0.16512934041320904 1106 30-31 -0.22817910648349482 1106 32-33 0.06231779208575716 1106 34-35 -0.34653240603663704 1106 36-37 -0.3384825084772345 1106 38-39 -0.15047243149117406 1106 40-41 0.056717433690671726 1106 42-43 -0.5396197357726535 1106 44-45 -0.28812372626700267 1106 46-47 -0.1335273429284669 1106 48-49 0.11277079374733034 1106 50-51 -0.027588413916717514 1106 52-53 -0.14559282780896865 1106 54-55 0.06758467232749865 1106 56-57 -0.8542273957413684 1106 58-59 -0.6581978966923998 1106 60-61 0.004889609404497008 1106 62-63 -0.11532214671648688 1106 64-65 -0.034346614084967086 1106 66-67 -0.3756026293248276 1106 68-69 -0.3601045333210813 1106 70-71 -0.46668554805957996 1106 72-73 -0.21186639799726592 1106 74-75 0.0781678576116569 1106 76-77 0.02063468743197916 1106 78-79 0.28143276449926447 1106 80-81 0.23981512549713102 1106 82-83 -0.05710265357389588 1106 84-85 0.12083110538442199 1106 86-87 0.16040209660918237 1106 88-89 0.3433589801021917 1106 90-91 0.2706452533070163 1106 92-93 0.13712718819989433 1106 94-95 0.16653846905447622 1106 96-97 0.1695527527089702 1106 98-99 0.5974359963241049 1106 100-101 0.23372299138782182 1107 1 0.182378329081466 1107 2 0.1486571778537744 1107 3 0.15461961388233547 1107 4 0.19167215919425473 1107 5 0.15685229885930596 1107 6 0.3164324049348437 1107 7 0.27184211224719945 1107 8 0.21264696620171009 1107 9 0.3346328181606779 1107 10-11 0.39964799659850314 1107 12-13 0.3987111403516508 1107 14-15 0.5656359123999621 1107 16-17 0.5799820053973761 1107 18-19 0.4270538183121104 1107 20-21 0.4821302261274951 1107 22-23 0.35943505768349837 1107 24-25 0.3976594865582612 1107 26-27 0.3890706715039798 1107 28-29 0.35382092011598587 1107 30-31 0.48175688268357675 1107 32-33 0.5380649055111562 1107 34-35 0.5495768587678711 1107 36-37 0.48508183981687836 1107 38-39 0.4932094622213654 1107 40-41 0.3660882821706011 1107 42-43 0.6228416590953785 1107 44-45 0.4754209617095455 1107 46-47 0.43843887037262874 1107 48-49 0.4301111190769902 1107 50-51 0.4466167368515457 1107 52-53 0.609798488332352 1107 54-55 0.6435690764949271 1107 56-57 0.6216027480854436 1107 58-59 0.6013581816669458 1107 60-61 0.5740577248760239 1107 62-63 0.6388922201593488 1107 64-65 0.737617570177381 1107 66-67 0.8297287767776638 1107 68-69 0.7956258992699752 1107 70-71 0.9846987369285252 1107 72-73 0.9468544105426382 1107 74-75 0.7644518226523864 1107 76-77 1.1109363093814792 1107 78-79 1.048628761099021 1107 80-81 1.19071505757773 1107 82-83 0.8490451202502207 1107 84-85 0.975542960523871 1107 86-87 1.3302388871397675 1107 88-89 1.3566527614188004 1107 90-91 1.100220842475796 1107 92-93 1.2362605750713769 1107 94-95 1.407390868232607 1107 96-97 1.656213531603548 1107 98-99 1.6391475332248397 1107 100-101 1.6349113673452091 1108 1 0.33527814609793083 1108 2 0.30726353025475817 1108 3 0.21466794081827345 1108 4 0.3481370418216656 1108 5 0.273724445390755 1108 6 0.3213956575826842 1108 7 0.2584665380384408 1108 8 0.2861203793568379 1108 9 0.34865194807497346 1108 10-11 0.5431279276633205 1108 12-13 0.43592594414148067 1108 14-15 0.6817001494630617 1108 16-17 0.64459854341316 1108 18-19 0.6063668366566688 1108 20-21 0.5517966879168696 1108 22-23 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-1.7880267486544632 2316 76-77 -2.0121837148325596 2316 78-79 -1.9367511615361543 2316 80-81 -2.1857481105499765 2316 82-83 -2.3540116180117714 2316 84-85 -2.2615776706640354 2316 86-87 -2.375591754167079 2316 88-89 -2.746942950164744 2316 90-91 -2.7477746425474514 2316 92-93 -2.8361227224358956 2316 94-95 -3.2923932421976865 2316 96-97 -3.4853939185949763 2316 98-99 -3.4290586804672856 2316 100-101 -2.957970484541807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9354.0 3 2797.0 4 2902.0 5 3372.0 6 4068.0 7 5313.0 8 6688.0 9 8670.0 10 11539.0 11 14705.0 12 19083.0 13 22767.0 14 26850.0 15 31857.0 16 38516.0 17 45120.0 18 41296.0 19 36352.0 20 37925.0 21 40685.0 22 45156.0 23 49858.0 24 56482.0 25 62636.0 26 70879.0 27 81287.0 28 93158.0 29 107882.0 30 124030.0 31 145276.0 32 172252.0 33 207161.0 34 253594.0 35 312603.0 36 364889.0 37 351792.0 38 160898.0 39 8078.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.03369710943892 18.548942107544548 14.62417636978178 22.79318441323475 2 15.608455885342384 20.2378888583514 39.90853931232101 24.24511594398521 3 19.097069971205855 24.478374464419776 29.898387762032584 26.52616780234178 4 12.666127546399908 16.58029031821541 37.041400793196416 33.712181342188266 5 13.419553620939674 37.90333183203901 33.80349662729457 14.873617919726748 6 31.92255305752354 36.19366093660156 17.362890825217836 14.520895180657062 7 28.775151180896103 33.39746625140602 20.14080208830648 17.686580479391395 8 27.162792934916496 33.73058665309447 19.89497146133191 19.211648950657125 9 27.419459149228103 16.27739116898345 20.076138082008008 36.22701159978044 10-11 25.02006141059064 26.491659540673744 27.116005421660212 21.3722736270754 12-13 26.532989004694535 24.13861763364434 27.23451959620705 22.093873765454074 14-15 22.930480216307533 25.528796614661665 26.025836588622596 25.514886580408202 16-17 21.913911629390697 28.648563354793378 27.15537407572502 22.282150940090908 18-19 22.0759117458876 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22.692998548944797 44-45 21.950434518422966 26.57174793544855 29.329692985805245 22.148124560323247 46-47 22.680588416695834 26.962464047719088 28.115877885565567 22.24106965001951 48-49 22.10747447176663 27.03929357671468 28.030804474447397 22.8224274770713 50-51 22.47890705130236 27.26883540083304 27.993219842213325 22.259037705651274 52-53 22.906791072614617 26.68260950942533 28.143314664209225 22.26728475375083 54-55 22.034718666949807 27.148136889221348 28.44613469158234 22.371009752246504 56-57 22.276893110623167 27.36196273165171 27.886642946762148 22.474501210962977 58-59 22.191040538788293 26.67132360802788 27.91719353799827 23.22044231518556 60-61 21.508828798144673 27.04380859649214 28.551360166605132 22.896002438758053 62-63 21.900479072776623 26.987349398177617 28.651626062624878 22.460545466420875 64-65 21.570764415394724 27.156482612005107 28.258227301048827 23.014525671551343 66-67 21.521596366329277 27.07665367209553 28.358368760518083 23.043381201057112 68-69 22.42300850246547 26.421105598343274 28.50087971946462 22.65500617972663 70-71 22.052787610770473 26.70585021490662 27.982321901494135 23.25904027282878 72-73 21.97472086627858 26.913692433468846 27.934887404812432 23.176699295440145 74-75 22.037086420306807 27.3800673125565 27.70286597566562 22.87998029147107 76-77 22.237512973170336 26.724609025572928 28.042408515892063 22.995469485364673 78-79 22.322012860628863 27.08049427965207 27.799851183223346 22.797641676495722 80-81 22.618378617594896 27.12079436849547 27.467605185769695 22.793221828139945 82-83 22.470255889035034 27.086685529948166 27.66093468123047 22.782123899786324 84-85 22.368643799317976 27.242807312829292 27.623931596160002 22.76461729169273 86-87 22.705394306830872 27.286725503251674 27.088615903945524 22.91926428597193 88-89 22.50504655211726 27.221072054899466 26.97186883401203 23.302012558971246 90-91 22.386732670918125 27.1336985874828 27.150908750222037 23.328659991377044 92-93 22.707187276354354 26.670570004296597 27.450490013500804 23.17175270584825 94-95 22.413552948580275 26.483692607351088 27.65296322439851 23.44979121967013 96-97 22.884506992809232 25.922005456946103 27.627593658396734 23.56589389184793 98-99 22.570562358757876 26.245867852866233 27.185827224859494 23.9977425635164 100-101 23.256399989009754 25.6018907124134 27.510177763969523 23.631531534607326 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 728.0 1 500.5 2 397.0 3 550.0 4 500.5 5 501.0 6 829.0 7 1784.0 8 2963.5 9 3419.0 10 3015.0 11 2553.5 12 2542.0 13 2755.5 14 3060.5 15 3392.0 16 3703.0 17 4133.0 18 4652.5 19 5234.5 20 5975.5 21 6955.0 22 8319.0 23 9964.0 24 12045.5 25 15170.5 26 19119.5 27 23468.0 28 27873.0 29 32216.0 30 36981.5 31 45344.5 32 59041.5 33 64363.0 34 62240.5 35 64598.0 36 70043.0 37 75349.5 38 79042.0 39 81938.0 40 85384.0 41 91037.0 42 95387.5 43 98173.0 44 100063.0 45 105126.5 46 108320.0 47 109582.0 48 111298.0 49 108909.5 50 107063.5 51 104380.5 52 101520.0 53 102826.0 54 105313.0 55 100921.5 56 92860.5 57 86164.0 58 81933.0 59 73523.0 60 58305.0 61 47255.5 62 40234.5 63 30024.0 64 20674.5 65 15912.0 66 12167.5 67 9282.0 68 7015.0 69 5072.0 70 3706.5 71 2676.5 72 2048.0 73 1765.5 74 1416.5 75 1065.5 76 791.5 77 557.0 78 378.5 79 244.5 80 165.0 81 103.0 82 60.0 83 44.0 84 29.5 85 16.0 86 14.5 87 15.5 88 14.0 89 9.5 90 6.5 91 3.5 92 3.0 93 2.5 94 0.5 95 1.0 96 1.0 97 1.0 98 1.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00766788941343895 2 0.10478365829805346 3 0.11482339486056463 4 0.11781257208953236 5 0.15810148256692344 6 0.09727822416879753 7 0.026870104003872932 8 0.028982022698252303 9 0.023685980433885572 10-11 0.0516770258986214 12-13 0.05325284215519678 14-15 0.02776360806687959 16-17 0.03140260643257943 18-19 0.036763630810619376 20-21 0.059929754335119254 22-23 0.03190621781354682 24-25 0.04977629907367997 26-27 0.0572979787313542 28-29 0.0539351543487655 30-31 0.0686860941525845 32-33 0.05029615598306566 34-35 0.06252903888204771 36-37 0.05378894459300078 38-39 0.03265351212078875 40-41 0.037673380402044335 42-43 0.033904417808998076 44-45 0.007310487788236288 46-47 0.013873681269230644 48-49 0.009584861766798689 50-51 0.008463920305935792 52-53 0.01033215607404062 54-55 0.012232882898982056 56-57 0.015173323542694872 58-59 0.02089174954593748 60-61 0.025797899128264946 62-63 0.0119242178590343 64-65 0.009341178840524145 66-67 0.010754539812916495 68-69 0.011972954444289209 70-71 0.031467588546252645 72-73 0.02873833977197776 74-75 0.030200437329625022 76-77 0.024254573928526174 78-79 0.005198569093856916 80-81 0.005052359338092191 82-83 0.006043336571608665 84-85 0.005361024378039944 86-87 0.01406862761025028 88-89 0.037835835686227365 90-91 0.035723916991847994 92-93 0.029761808062330843 94-95 0.0471282779414966 96-97 0.04301815925166598 98-99 0.026918840589127843 100-101 0.07523304210516055 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3077770.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.351851600443915 #Duplication Level Percentage of deduplicated Percentage of total 1 73.06981333658355 33.86921144249458 2 13.732587945513842 12.730617570810052 3 5.156318609506137 7.170147449773072 4 2.5792564280322474 4.7821324476656715 5 1.484558428609174 3.4406015987540326 6 0.8980377634609491 2.497542788612188 7 0.6039974072992119 1.9597478733130165 8 0.426740758138594 1.5824179434480834 9 0.303233072175135 1.2649872925627703 >10 1.5525307639733343 12.81468116491279 >50 0.1133964264882119 3.650517645427872 >100 0.0681694562056433 6.048933413796063 >500 0.006208976107167465 1.905612160080802 >1k 0.004727288626833097 4.708792895083183 >5k 2.8222618667660284E-4 0.7969375491723703 >10k+ 1.4111309333830142E-4 0.7771187640936417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13527 0.4395065258287656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10235 0.33254596672265957 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7102 0.23075148565357387 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6000 0.19494634101963434 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5954 0.19345175240515047 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5312 0.1725924939160496 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4794 0.15576212647468785 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4690 0.15238305656368084 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4645 0.15092095900603358 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4177 0.13571514440650212 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4085 0.13272596717753438 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3879 0.1260328094691936 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3815 0.12395338183165085 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3749 0.12180897208043485 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3675 0.11940463387452604 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3603 0.11706527778229042 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3398 0.11040461113078624 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3362 0.10923493308466845 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.8736585254908585E-5 1.1371869892812004E-4 14-15 0.0 0.0 0.0 9.747317050981717E-5 1.624552841830286E-4 16-17 0.0 0.0 0.0 9.747317050981717E-5 1.9494634101963434E-4 18-19 0.0 0.0 0.0 1.299642273464229E-4 2.9241951152945153E-4 20-21 0.0 1.6245528418302863E-5 0.0 2.436829262745429E-4 4.386292672941773E-4 22-23 0.0 3.2491056836605725E-5 0.0 8.285219493334461E-4 4.5487479571248014E-4 24-25 0.0 3.2491056836605725E-5 0.0 0.0015433251997387719 4.5487479571248014E-4 26-27 0.0 3.2491056836605725E-5 0.0 0.0025992845469284577 5.036113809673888E-4 28-29 0.0 3.2491056836605725E-5 0.0 0.006904349577778716 5.198569093856916E-4 30-31 0.0 3.2491056836605725E-5 0.0 0.021769008080525835 5.198569093856916E-4 32-33 0.0 3.2491056836605725E-5 0.0 0.051400851915510255 5.523479662222973E-4 34-35 0.0 3.2491056836605725E-5 0.0 0.09604356400900652 5.848390230589031E-4 36-37 0.0 3.2491056836605725E-5 0.0 0.16961956221550017 5.848390230589031E-4 38-39 0.0 3.2491056836605725E-5 0.0 0.2781559375781816 5.848390230589031E-4 40-41 0.0 3.2491056836605725E-5 0.0 0.3952537064173086 5.848390230589031E-4 42-43 0.0 3.2491056836605725E-5 0.0 0.5163478752473382 6.010845514772059E-4 44-45 0.0 3.2491056836605725E-5 0.0 0.6700793106697381 6.335756083138116E-4 46-47 0.0 3.2491056836605725E-5 0.0 0.8500797655445339 6.660666651504174E-4 48-49 0.0 3.2491056836605725E-5 0.0 1.0358798740646638 7.310487788236288E-4 50-51 0.0 3.2491056836605725E-5 0.0 1.2383478947419722 7.472943072419317E-4 52-53 0.0 3.2491056836605725E-5 0.0 1.4378104926618946 7.472943072419317E-4 54-55 0.0 3.2491056836605725E-5 0.0 1.6552406450124604 7.472943072419317E-4 56-57 0.0 3.2491056836605725E-5 0.0 1.9045607696481544 7.635398356602345E-4 58-59 0.0 3.2491056836605725E-5 0.0 2.1609152080889737 7.797853640785374E-4 60-61 0.0 3.2491056836605725E-5 0.0 2.447778748899365 7.797853640785374E-4 62-63 0.0 3.2491056836605725E-5 0.0 2.7315881303671166 7.797853640785374E-4 64-65 0.0 6.498211367321145E-5 0.0 3.0250473557153392 8.447674777517488E-4 66-67 0.0 6.498211367321145E-5 0.0 3.368104179324641 8.610130061700518E-4 68-69 0.0 6.498211367321145E-5 0.0 3.7468849199257903 8.772585345883546E-4 70-71 0.0 6.498211367321145E-5 0.0 4.134178317418131 8.772585345883546E-4 72-73 0.0 6.498211367321145E-5 0.0 4.538188363652905 9.259951198432631E-4 74-75 0.0 6.498211367321145E-5 0.0 4.947364487924698 0.0010072227619347775 76-77 0.0 6.498211367321145E-5 0.0 5.393271751950276 0.0010722048756079889 78-79 0.0 6.498211367321145E-5 0.0 5.888337984969637 0.0010884504040262917 80-81 0.0 6.498211367321145E-5 0.0 6.400559495998726 0.0011046959324445946 82-83 0.0 6.498211367321145E-5 0.0 6.928214259025204 0.0011371869892812002 84-85 0.0 6.498211367321145E-5 0.0 7.478157887041592 0.0011696780461178061 86-87 0.0 6.498211367321145E-5 0.0 8.0405455898264 0.001185923574536109 88-89 0.0 9.747317050981717E-5 0.0 8.624149952725512 0.0012184146313727147 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7345 0.0 53.87527 1 GTATCAA 13320 0.0 42.455658 1 TATCAAC 17380 0.0 32.509514 2 ATCAACG 17420 0.0 32.35296 3 TCAACGC 17790 0.0 31.599874 4 CAACGCA 18070 0.0 31.031263 5 AACGCAG 18715 0.0 29.965258 6 ACGCAGA 21125 0.0 26.497862 7 GTACATG 16185 0.0 26.183212 1 CGCAGAG 21360 0.0 26.094679 8 TAAGGTG 2115 0.0 26.085035 5 TACATGG 16060 0.0 25.941933 2 GGACCGA 1055 0.0 25.226755 6 TGGTATC 3305 0.0 24.607555 2 ACATGGG 16460 0.0 24.3291 3 GCAGAGT 22710 0.0 23.977365 9 GTGGTAT 3640 0.0 23.78088 1 ACCGAGT 1105 0.0 23.651333 8 AGGACCG 1260 0.0 22.64772 5 GTGTAAG 1285 0.0 22.577957 1 >>END_MODULE