FastQCFastQC Report
Thu 26 May 2016
SRR938663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938663_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3077770
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130800.4249830234228029No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106070.34463263986587694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74320.24147353440965377No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA61970.20134707921644568No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA58590.19036510200567294No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA54480.177011277645828No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC51990.16892100449351316No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48430.15735418825968153No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47170.1532603150982692No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46580.15134334274490946No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38460.12496060459358561No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT38120.12385590866114103No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG37680.12242630216033037No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG37330.12128911517104916No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36420.11833242899891804No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA35960.11683784038443419No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT35400.11501834120158426No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT34920.11345877047342719No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA33120.10761038024283817No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73250.055.482071
GTATCAA129500.041.4398731
TATCAAC172650.031.0003072
ATCAACG174400.030.563323
TCAACGC177600.030.0126294
TAAGGTG23800.029.3423275
CAACGCA182450.029.2148175
AACGCAG189950.028.1485276
GGTAAGG25700.026.4336453
AGGTAAG23200.026.4299242
GTAAGGT26000.026.311364
GTACATG156000.024.92431
ACGCAGA214200.024.8065957
TACATGG154550.024.6968042
CGCAGAG216350.024.5381328
TGGTATC32400.024.2065622
GTGGTAT34850.023.5959531
ACATGGG158100.023.2575113
AAGGTAA24850.022.9535081
GCAGAGT234650.022.2399449
Produced by FastQC (version 0.11.5)