##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938663_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3077770 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82158933253622 31.0 31.0 34.0 30.0 34.0 2 32.04119313658915 33.0 31.0 34.0 30.0 34.0 3 32.09680417964955 33.0 31.0 34.0 30.0 34.0 4 35.77275137518398 37.0 35.0 37.0 35.0 37.0 5 35.670901659318275 37.0 35.0 37.0 33.0 37.0 6 35.67825113637471 37.0 35.0 37.0 33.0 37.0 7 35.617984449780195 37.0 35.0 37.0 33.0 37.0 8 35.59236232726942 37.0 35.0 37.0 33.0 37.0 9 37.178262183334034 39.0 37.0 39.0 33.0 39.0 10-11 37.08477452831109 39.0 37.0 39.0 33.0 39.0 12-13 36.982421850885544 39.0 37.0 39.0 33.0 39.0 14-15 38.023071737004386 40.0 37.0 41.0 33.0 41.0 16-17 37.94841281187353 40.0 37.0 41.0 33.0 41.0 18-19 38.19948469183857 40.0 37.5 41.0 33.0 41.0 20-21 38.177778553953026 40.0 37.5 41.0 33.0 41.0 22-23 38.077056927580685 40.0 37.0 41.0 33.0 41.0 24-25 37.9456245918311 40.0 37.0 41.0 33.0 41.0 26-27 37.75401963109654 40.0 37.0 41.0 32.0 41.0 28-29 37.638615621050306 40.0 37.0 41.0 32.0 41.0 30-31 37.36600948089038 39.0 36.0 41.0 31.5 41.0 32-33 37.13627301585238 39.0 36.0 41.0 31.0 41.0 34-35 37.0158398775737 39.0 36.0 41.0 31.0 41.0 36-37 36.944514859784846 39.0 36.0 41.0 31.0 41.0 38-39 36.7641995665693 39.0 35.0 41.0 30.0 41.0 40-41 36.57276843298882 39.0 35.0 41.0 30.0 41.0 42-43 36.33233055101583 39.0 35.0 40.5 30.0 41.0 44-45 35.99715849462436 39.0 35.0 40.0 29.5 41.0 46-47 35.56681071035197 38.0 35.0 40.0 27.5 41.0 48-49 35.160456596821724 38.0 34.0 40.0 26.5 41.0 50-51 34.771514603105494 38.0 34.0 40.0 25.5 41.0 52-53 34.291706332831886 38.0 33.0 40.0 24.0 41.0 54-55 33.953797879633626 37.5 33.0 40.0 23.0 41.0 56-57 33.61651845329573 37.0 33.0 40.0 23.0 41.0 58-59 33.15636418575787 36.5 32.0 39.5 21.5 41.0 60-61 32.76326219957957 36.0 31.5 39.0 20.0 40.0 62-63 32.30282184828626 35.5 31.0 39.0 18.5 40.0 64-65 31.798011872232166 35.0 31.0 38.5 17.0 40.0 66-67 31.31974578997131 35.0 30.0 38.0 13.0 40.0 68-69 30.85133684453354 34.5 29.5 37.5 9.0 40.0 70-71 30.31040818514704 34.0 29.0 37.0 7.5 39.0 72-73 29.854840680102804 34.0 29.0 36.0 7.0 39.0 74-75 29.30776731204736 34.0 28.0 36.0 3.5 38.5 76-77 28.068651978542906 31.5 26.5 34.5 2.0 36.5 78-79 28.769353947825863 33.5 28.0 35.0 2.0 37.0 80-81 28.44457139422374 33.0 27.0 35.0 2.0 37.0 82-83 28.037996341507004 33.0 26.5 35.0 2.0 36.0 84-85 27.53101352602696 33.0 26.0 35.0 2.0 36.0 86-87 26.98246343943829 32.5 25.5 35.0 2.0 35.5 88-89 26.273813832742537 32.0 24.5 34.0 2.0 35.0 90-91 25.716489048889294 32.0 23.0 34.0 2.0 35.0 92-93 24.96531449718465 31.0 19.5 34.0 2.0 35.0 94-95 24.22521793376373 31.0 14.5 34.0 2.0 35.0 96-97 23.244409263850123 31.0 2.0 34.0 2.0 35.0 98-99 21.97339908440202 30.0 2.0 34.0 2.0 35.0 100-101 18.05341448516296 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24078903300747712 1101 2 0.298901157996049 1101 3 0.3124035815114965 1101 4 0.2560111796791489 1101 5 0.1731786485024287 1101 6 0.2426599129536342 1101 7 0.28465958710133066 1101 8 0.22064104719925837 1101 9 0.2763961683757401 1101 10-11 0.341158065300057 1101 12-13 0.42020468611791983 1101 14-15 0.4213756697959852 1101 16-17 0.47613950500238644 1101 18-19 0.4540303102201406 1101 20-21 0.38593448151445386 1101 22-23 0.3989120723636148 1101 24-25 0.49140833822567487 1101 26-27 0.3558437014137965 1101 28-29 0.35169614075286404 1101 30-31 0.47175921186582315 1101 32-33 0.44128179160864534 1101 34-35 0.35454776062433524 1101 36-37 0.5452242767024202 1101 38-39 0.739104728897594 1101 40-41 0.7113236820239663 1101 42-43 0.766420265646957 1101 44-45 0.7391377220548208 1101 46-47 0.5517469824417773 1101 48-49 0.520895582828949 1101 50-51 0.48080482808205716 1101 52-53 0.63426445970331 1101 54-55 0.5618791460688897 1101 56-57 0.46405265681478625 1101 58-59 0.684224080070031 1101 60-61 0.6200531117865609 1101 62-63 0.7286033896458619 1101 64-65 0.5997317604097283 1101 66-67 0.5038430726935381 1101 68-69 0.6411415555376294 1101 70-71 0.6969437604220801 1101 72-73 0.6820664028341916 1101 74-75 0.6684225520540714 1101 76-77 0.6473201334741496 1101 78-79 0.7436773527334992 1101 80-81 0.7509472605509373 1101 82-83 0.7415547623342782 1101 84-85 0.9309877001518387 1101 86-87 0.8374591664432991 1101 88-89 0.7950683922512702 1101 90-91 0.7919482578125354 1101 92-93 1.0043596996217268 1101 94-95 0.9465774209459319 1101 96-97 0.9558658524476016 1101 98-99 1.1530629087646034 1101 100-101 1.2979380433414924 1102 1 0.2650295100101623 1102 2 0.254209775595001 1102 3 0.23405192983416612 1102 4 0.22868645122226638 1102 5 0.07540255922279471 1102 6 0.09394945444118008 1102 7 0.07493856832579837 1102 8 0.1971141173678106 1102 9 0.17715375949220658 1102 10-11 0.2554582146498703 1102 12-13 0.2427645149253621 1102 14-15 0.29641500524072484 1102 16-17 0.30928104590581285 1102 18-19 0.28809048617848276 1102 20-21 0.25709507032235734 1102 22-23 0.3178581380698944 1102 24-25 0.38560458617516247 1102 26-27 0.3123800550041054 1102 28-29 0.3181240181314635 1102 30-31 0.3257800509103248 1102 32-33 0.27156738089400534 1102 34-35 0.15619266535102128 1102 36-37 0.27021163500300815 1102 38-39 0.30648498680882597 1102 40-41 0.1893213220441865 1102 42-43 0.21637882202806225 1102 44-45 0.3269932726056055 1102 46-47 0.2725958552958119 1102 48-49 0.12915003225172228 1102 50-51 0.15089342134665884 1102 52-53 0.031807489227446695 1102 54-55 0.09395703597900962 1102 56-57 0.09163399487513146 1102 58-59 0.13252364295077257 1102 60-61 0.14308502469530993 1102 62-63 0.2802859036119614 1102 64-65 0.2519072639254567 1102 66-67 0.03502135253507177 1102 68-69 -0.007962183966675696 1102 70-71 5.75196927545818E-4 1102 72-73 0.03773088316999562 1102 74-75 0.029197995806370614 1102 76-77 -0.06593108537371961 1102 78-79 0.12127301462525963 1102 80-81 0.16774746939974605 1102 82-83 0.2498722072330679 1102 84-85 0.14992937321648014 1102 86-87 -0.11743912847719074 1102 88-89 -0.1833255404381795 1102 90-91 0.034343405162889695 1102 92-93 0.4885547105798267 1102 94-95 0.5545644921822372 1102 96-97 0.5122778017256628 1102 98-99 0.5838621286079402 1102 100-101 0.8704870741837105 1103 1 0.29766791075622123 1103 2 0.32750297347632795 1103 3 0.32916680889379535 1103 4 0.26171340109380736 1103 5 0.1459356050242917 1103 6 0.2660675311476908 1103 7 0.33540509325566603 1103 8 0.277884897925162 1103 9 0.4456017196161497 1103 10-11 0.3777503501221702 1103 12-13 0.43987436204074726 1103 14-15 0.5573822293259454 1103 16-17 0.5255655318686152 1103 18-19 0.48891746240116873 1103 20-21 0.49680739589854994 1103 22-23 0.38861795178019065 1103 24-25 0.4554229154660234 1103 26-27 0.5429868401357609 1103 28-29 0.3841115601551621 1103 30-31 0.3391701200937334 1103 32-33 0.4152508320876933 1103 34-35 0.44602775956322205 1103 36-37 0.5549561049988299 1103 38-39 0.5035383476010935 1103 40-41 0.5580760358072823 1103 42-43 0.5443826296449714 1103 44-45 0.4948958517958033 1103 46-47 0.5948987259115555 1103 48-49 0.5470514803444217 1103 50-51 0.7006535532723888 1103 52-53 0.65371114412563 1103 54-55 0.6474026262823358 1103 56-57 0.7094821511382392 1103 58-59 0.6142839761433052 1103 60-61 0.6703189045338291 1103 62-63 0.7363249427905743 1103 64-65 0.7290210486311288 1103 66-67 0.6898575620779432 1103 68-69 0.7696131844866194 1103 70-71 0.6517022293212165 1103 72-73 0.8417131278185686 1103 74-75 0.8706954537161735 1103 76-77 0.8022049523383039 1103 78-79 1.0151735337098913 1103 80-81 1.0748308268974363 1103 82-83 1.13633764548673 1103 84-85 1.035111324300697 1103 86-87 0.9524245132646136 1103 88-89 1.0105196851170568 1103 90-91 1.0932059241704692 1103 92-93 1.3523301275400677 1103 94-95 1.1961150362675532 1103 96-97 1.5342035438730015 1103 98-99 1.8430324843662085 1103 100-101 2.1391046989310816 1104 1 0.13054445836888817 1104 2 0.19797243040724055 1104 3 0.19876700558940286 1104 4 0.17372980538429061 1104 5 0.058081097215819 1104 6 0.10582355373281871 1104 7 0.17087878175720306 1104 8 0.17659255754229264 1104 9 0.21833407335135746 1104 10-11 0.17982578104116698 1104 12-13 0.26117389135061586 1104 14-15 0.3571913792096879 1104 16-17 0.2827289489471312 1104 18-19 0.25768090798927545 1104 20-21 0.2657235164520344 1104 22-23 0.33956070366760116 1104 24-25 0.3009191834540488 1104 26-27 0.20862584575640142 1104 28-29 0.2153538703235185 1104 30-31 0.22804376854853814 1104 32-33 0.39115286424147655 1104 34-35 0.3837643833262163 1104 36-37 0.4209589539059877 1104 38-39 0.26477207156527527 1104 40-41 0.34705359553576187 1104 42-43 0.41891444564637936 1104 44-45 0.3467617167757453 1104 46-47 0.2194069548486226 1104 48-49 0.1408399295026328 1104 50-51 0.2869181775727583 1104 52-53 0.3432556394560322 1104 54-55 0.3209157374853433 1104 56-57 0.17945037013284804 1104 58-59 0.21620880878410986 1104 60-61 0.2703574770754571 1104 62-63 0.31384421431948084 1104 64-65 0.47358788373164984 1104 66-67 0.4483761457362583 1104 68-69 0.38248408145908996 1104 70-71 0.3961380536621988 1104 72-73 0.560986489844371 1104 74-75 0.5793373951571859 1104 76-77 0.5707575244435752 1104 78-79 0.8012670620603899 1104 80-81 0.8294694666622 1104 82-83 0.812244074576963 1104 84-85 0.9714222125767229 1104 86-87 0.999783749125772 1104 88-89 0.9694920377446934 1104 90-91 0.9598124865532576 1104 92-93 1.0084842490078039 1104 94-95 1.0245691375068198 1104 96-97 1.100244311549904 1104 98-99 1.0159439972379545 1104 100-101 1.0976237056182718 1105 1 0.18052493779420686 1105 2 0.21460600219190695 1105 3 0.17683573002495478 1105 4 0.15283642376006412 1105 5 0.10507522903075284 1105 6 0.10193565588848941 1105 7 0.17056176762430653 1105 8 0.1543093110004321 1105 9 0.27301438640130726 1105 10-11 0.2261655831926319 1105 12-13 0.3377791132545056 1105 14-15 0.35987717003322217 1105 16-17 0.24126670774490577 1105 18-19 0.3492666393501267 1105 20-21 0.35062324076145757 1105 22-23 0.22508676813955475 1105 24-25 0.28670808495622424 1105 26-27 0.312861269893439 1105 28-29 0.257398245094528 1105 30-31 0.3747602877597842 1105 32-33 0.4774169265631656 1105 34-35 0.4827893756681405 1105 36-37 0.399527585243419 1105 38-39 0.4548633677914964 1105 40-41 0.5520382493608977 1105 42-43 0.579812271955312 1105 44-45 0.4040288963830534 1105 46-47 0.5086389854088367 1105 48-49 0.6052334402629711 1105 50-51 0.6536097705117143 1105 52-53 0.5069716361165106 1105 54-55 0.4158310181912057 1105 56-57 0.3251970471304091 1105 58-59 0.47217039229312263 1105 60-61 0.577200789927204 1105 62-63 0.48681920996488515 1105 64-65 0.5781422440274007 1105 66-67 0.401582024732722 1105 68-69 0.6046540486044627 1105 70-71 0.5487254007260027 1105 72-73 0.6084353609545197 1105 74-75 0.6423935363299833 1105 76-77 0.5988897357943408 1105 78-79 0.7046399771128549 1105 80-81 0.8435392962625983 1105 82-83 0.8707596700145537 1105 84-85 0.7786255448889783 1105 86-87 0.8249497941372113 1105 88-89 0.9983285514301166 1105 90-91 1.037071317360926 1105 92-93 1.108659934179979 1105 94-95 1.2618865901018559 1105 96-97 1.3530384565787656 1105 98-99 1.577938579527686 1105 100-101 1.4242228010648752 1106 1 -0.009150114616698346 1106 2 0.06339640466155316 1106 3 0.06944164612144021 1106 4 0.06337205165417714 1106 5 -0.06402364903038915 1106 6 -0.10066761086572029 1106 7 0.0018346038028056455 1106 8 -0.005018792761632085 1106 9 -0.26989194207105527 1106 10-11 -0.014396307746615378 1106 12-13 -0.013361774159967865 1106 14-15 -0.015328902720021631 1106 16-17 0.1081716424328576 1106 18-19 0.07098001507294072 1106 20-21 0.1025162534091919 1106 22-23 0.13957083752928412 1106 24-25 0.029574500511323265 1106 26-27 0.0924196936499797 1106 28-29 0.0638604516510739 1106 30-31 0.01334772757364533 1106 32-33 0.060643595072853884 1106 34-35 -0.05229805262683129 1106 36-37 0.12197321613244583 1106 38-39 -0.0413003845986708 1106 40-41 0.1883854975005974 1106 42-43 0.22821103252388752 1106 44-45 0.05489016299904392 1106 46-47 -0.575775625137851 1106 48-49 -0.23528462133999284 1106 50-51 0.1728316126109135 1106 52-53 0.006846829057742809 1106 54-55 0.2236035548788422 1106 56-57 0.3657272035567871 1106 58-59 0.16683571125089003 1106 60-61 0.24503691082935575 1106 62-63 -0.08568055965752563 1106 64-65 -0.013616458486012561 1106 66-67 0.10982407094646618 1106 68-69 -0.06642089322519595 1106 70-71 -3.600578405880128E-4 1106 72-73 0.18149396901312898 1106 74-75 0.13105181725241266 1106 76-77 0.25922343773181566 1106 78-79 0.38457391633924587 1106 80-81 -0.20160751185590797 1106 82-83 0.3428289215177678 1106 84-85 0.4782738159051725 1106 86-87 0.6532349209600774 1106 88-89 0.783313346509253 1106 90-91 0.7183542901533322 1106 92-93 0.66516262959777 1106 94-95 0.7933719496060831 1106 96-97 0.9327470865232463 1106 98-99 0.898423639541047 1106 100-101 1.062930382791322 1107 1 0.12819178054821023 1107 2 0.1790867477186069 1107 3 0.14730344508855353 1107 4 0.10345774116426298 1107 5 0.09073359250211155 1107 6 0.07638673747110403 1107 7 0.09828143658010902 1107 8 0.16828685309208424 1107 9 0.19129414854795357 1107 10-11 0.11412364731551605 1107 12-13 0.1805512697826046 1107 14-15 0.33160404502475416 1107 16-17 0.2992845061113556 1107 18-19 0.2705095450621329 1107 20-21 0.25197396610584377 1107 22-23 0.35153725373425715 1107 24-25 0.2613723676712212 1107 26-27 0.2587114331128575 1107 28-29 0.19764341285158338 1107 30-31 0.19966228838286781 1107 32-33 0.19564088256842638 1107 34-35 0.18170521946512252 1107 36-37 0.3654825881556363 1107 38-39 0.25858043607274794 1107 40-41 0.3273751857120928 1107 42-43 0.27514216481293374 1107 44-45 0.4331626821253991 1107 46-47 0.3811279122242297 1107 48-49 0.4478549743900473 1107 50-51 0.4602076276642464 1107 52-53 0.41631672666603947 1107 54-55 0.39430258008591323 1107 56-57 0.26341991952126165 1107 58-59 0.4161746183892703 1107 60-61 0.3928359911408492 1107 62-63 0.44371492562446946 1107 64-65 0.5015737799153577 1107 66-67 0.4962479328693128 1107 68-69 0.514854923623087 1107 70-71 0.643630516740469 1107 72-73 0.5556562963846439 1107 74-75 0.5855490214506034 1107 76-77 0.6432688924714363 1107 78-79 0.783533606814725 1107 80-81 0.7686303875977458 1107 82-83 0.8271585484736583 1107 84-85 0.9924418993392479 1107 86-87 1.17296556072667 1107 88-89 0.9047010167883194 1107 90-91 1.0601834579057154 1107 92-93 1.2588976520880593 1107 94-95 1.544366712550282 1107 96-97 1.5366067987841916 1107 98-99 1.4886666329498723 1107 100-101 1.8104782360683274 1108 1 0.2178717903501841 1108 2 0.16443571198359308 1108 3 0.24005791974904866 1108 4 0.16844722613892316 1108 5 0.07591303085714429 1108 6 0.1296906849743138 1108 7 0.14367541358966207 1108 8 0.16170296086556846 1108 9 0.16994071313752102 1108 10-11 0.19706886875371765 1108 12-13 0.19807842104430762 1108 14-15 0.34843506545423253 1108 16-17 0.307434203113921 1108 18-19 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-0.8852623033883056 2316 74-75 -0.9169971446736014 2316 76-77 -0.9850753672030201 2316 78-79 -1.0942823908417694 2316 80-81 -1.0412730240335932 2316 82-83 -1.0414081979597647 2316 84-85 -1.1318222868491752 2316 86-87 -1.2348056117472233 2316 88-89 -1.2144537558683162 2316 90-91 -1.0421303053890831 2316 92-93 -1.4197834861989271 2316 94-95 -1.400600313675369 2316 96-97 -1.3980761993745965 2316 98-99 -1.5305595889704833 2316 100-101 -1.790348076086648 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 24.0 4 52.0 5 108.0 6 257.0 7 654.0 8 1528.0 9 2761.0 10 4633.0 11 7339.0 12 10210.0 13 13990.0 14 17847.0 15 23508.0 16 30728.0 17 38534.0 18 35337.0 19 28163.0 20 26966.0 21 27770.0 22 29912.0 23 33529.0 24 38702.0 25 45506.0 26 53841.0 27 64788.0 28 77564.0 29 93079.0 30 110520.0 31 132195.0 32 159654.0 33 197257.0 34 253930.0 35 335120.0 36 440027.0 37 489838.0 38 241312.0 39 10583.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63543223197932 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21.941681802083977 26.552114030613073 29.933523297712306 21.572680869590645 20-21 22.53225549667454 25.667008905798678 30.20662687595239 21.594108721574386 22-23 22.3584283425987 25.919643118231704 30.102493038791074 21.61943550037852 24-25 22.032964810208885 26.194109641275237 30.10757404946037 21.6653514990555 26-27 21.334012410211173 26.553514263457462 29.86713324771766 22.245340078613708 28-29 21.616330405670602 26.95221017308647 29.755410414300233 21.676049006942694 30-31 21.59596375751613 26.429242613186283 29.5031799575919 22.471613671705686 32-33 21.119390776619774 26.55375859250632 29.534844315880292 22.79200631499361 34-35 22.414095645121037 26.573499630854336 29.26137397575077 21.751030748273852 36-37 22.53641327883224 25.89713528019558 29.255826083989206 22.31062535698297 38-39 21.712935043397113 26.326835246930745 29.266213156642916 22.69401655302922 40-41 22.360225945378666 26.192329748987486 29.413903406238678 22.03354089939517 42-43 22.006290686512063 26.261886013514207 29.06379372162778 22.66802957834595 44-45 21.922330683873803 26.552673077316758 29.3922825893758 22.132713649433644 46-47 22.643504875283078 26.93617456795017 28.18904596509811 22.231274591668644 48-49 22.109611829343972 27.103178600090327 27.96914649242796 22.818063078137744 50-51 22.44070791313331 27.257667572468918 28.057391266770974 22.244233247626795 52-53 22.923122910418908 26.660812861259934 28.145605422107568 22.27045880621359 54-55 22.02292219295088 27.17096054346795 28.37649737662604 22.42961988695513 56-57 22.315913794728 27.267323419228855 27.85065160814486 22.566111177898286 58-59 22.12261671369528 26.775500056466832 27.82510068564548 23.276782544192407 60-61 21.47249469583497 27.195696884432557 28.38114933864454 22.95065908108793 62-63 21.810418570645222 27.11078616543797 28.50938483722253 22.569410426694287 64-65 21.453010922961486 27.25217635450019 28.11534150787108 23.179471214667245 66-67 21.44078899483223 27.24120855125659 28.17958912584613 23.13841332806505 68-69 22.29709265634617 26.606503485913684 28.237395693936325 22.85900816380382 70-71 21.9090135950815 26.863627613196904 27.796349139433048 23.43100965228855 72-73 21.757215254238666 27.039745529836157 27.73523341188053 23.46780580404465 74-75 21.81054057400504 27.43912168707941 27.462910466120327 23.287427272795224 76-77 21.83732695112982 26.844246874250814 28.05443293607096 23.2639932385484 78-79 21.962749864226748 27.08612233210837 27.82826820279763 23.122859600867258 80-81 22.069554659212525 27.217140886729606 27.55235953040465 23.16094492365322 82-83 21.927847315441696 27.226059343380992 27.63205023613192 23.21404310504539 84-85 21.7640728706825 27.267784858573023 27.654151364225214 23.31399090651926 86-87 22.021726577607474 27.344286415706097 27.14431429612186 23.489672710564566 88-89 21.731134407756844 27.306211025509658 26.975269810418517 23.987384756314977 90-91 21.541403770366813 27.206378930769574 27.303829581448074 23.948387717415535 92-93 21.7013947003661 26.776280831522858 27.54049798424117 23.981826483869867 94-95 21.297875116777494 26.540833244905542 27.7745755994226 24.38671603889436 96-97 21.637866082810724 26.051841050940695 27.788505856219842 24.52178701002874 98-99 21.27902041830071 26.41996483521199 27.332324801448333 24.968689945038967 100-101 21.21066971005488 26.092217392547685 27.829909088309385 24.86720380908805 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 649.0 1 458.0 2 375.5 3 513.5 4 503.0 5 518.0 6 890.0 7 1893.0 8 2964.5 9 3289.5 10 2918.0 11 2530.5 12 2528.0 13 2717.0 14 3019.0 15 3313.0 16 3690.5 17 4128.5 18 4620.0 19 5200.5 20 5919.0 21 6870.5 22 8153.5 23 9817.5 24 12021.0 25 15048.5 26 18992.0 27 23381.5 28 27838.5 29 32224.0 30 36986.0 31 45298.0 32 59471.5 33 65002.0 34 62011.0 35 64350.5 36 70037.5 37 75372.5 38 78840.0 39 81924.5 40 85674.5 41 91681.5 42 96097.0 43 98583.5 44 100981.0 45 106256.0 46 109228.5 47 111111.0 48 112757.5 49 109781.0 50 108097.5 51 105175.0 52 101442.5 53 103177.0 54 107117.5 55 102204.0 56 93636.5 57 86207.5 58 81220.0 59 72725.5 60 57102.5 61 45979.5 62 39518.0 63 28950.5 64 18812.0 65 14481.0 66 11120.5 67 8551.0 68 6516.0 69 4631.0 70 3323.0 71 2261.0 72 1682.5 73 1569.5 74 1256.5 75 897.5 76 677.5 77 475.0 78 300.0 79 201.5 80 148.0 81 92.5 82 44.0 83 23.5 84 15.0 85 4.5 86 4.0 87 5.0 88 6.0 89 7.0 90 3.5 91 1.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.223837388758744E-4 2 0.05292793158683072 3 0.0 4 0.0 5 0.04590986331012389 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.007001822748288533 26-27 0.002030691052287858 28-29 6.498211367321145E-5 30-31 0.0018194991828499206 32-33 5.036113809673887E-4 34-35 0.012801476393622656 36-37 0.02493688612209489 38-39 0.004743694298144436 40-41 0.0011371869892812005 42-43 0.0014783430860655603 44-45 0.009324933312105843 46-47 0.0 48-49 0.0 50-51 6.335756083138116E-4 52-53 0.0 54-55 0.0017545170691767092 56-57 0.0 58-59 0.024124609701179748 60-61 0.0 62-63 0.010803276398171403 64-65 0.01595310890677341 66-67 0.00760290729976574 68-69 0.017155278009727822 70-71 8.122764209151432E-5 72-73 8.122764209151432E-5 74-75 0.02238633816042134 76-77 0.04898026818118313 78-79 0.03083401293793883 80-81 0.026740139776526513 82-83 0.035740162520266294 84-85 0.01658668451508722 86-87 0.011079450381282552 88-89 0.0845254843604298 90-91 0.026902595060709543 92-93 0.02485565848000338 94-95 0.02831595603310189 96-97 0.03937916088596614 98-99 0.05463371207075253 100-101 0.016180546304629652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3077770.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.82620295524853 #Duplication Level Percentage of deduplicated Percentage of total 1 70.87892536119631 31.063541681297018 2 14.629088377910362 12.82274792601134 3 5.643052082178313 7.419406375217538 4 2.864136418301954 5.020968958400802 5 1.5570298475353117 3.4119353052731123 6 0.9867526288314669 2.5947372586675734 7 0.6707628429241923 2.0577891942184605 8 0.4626673168838819 1.6221561384410639 9 0.3462482055045298 1.3657269714598912 >10 1.7426201816441593 13.645496794836756 >50 0.12863319342693488 3.907383183403494 >100 0.07716509986068666 6.450049832031094 >500 0.007424392989748157 2.161551501618692 >1k 0.004974343302919916 4.705232744242243 >5k 3.712196494716355E-4 0.9805415318018614 >10k+ 1.484878597886542E-4 0.7707346030791669 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13080 0.4249830234228029 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10607 0.34463263986587694 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7432 0.24147353440965377 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6197 0.20134707921644568 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5859 0.19036510200567294 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5448 0.177011277645828 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5199 0.16892100449351316 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4843 0.15735418825968153 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4717 0.1532603150982692 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4658 0.15134334274490946 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3846 0.12496060459358561 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3812 0.12385590866114103 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3768 0.12242630216033037 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3733 0.12128911517104916 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3642 0.11833242899891804 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3596 0.11683784038443419 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3540 0.11501834120158426 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3492 0.11345877047342719 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3312 0.10761038024283817 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.2491056836605725E-5 2 0.0 0.0 0.0 0.0 3.2491056836605725E-5 3 0.0 0.0 0.0 0.0 3.2491056836605725E-5 4 0.0 0.0 0.0 0.0 3.2491056836605725E-5 5 0.0 0.0 0.0 0.0 9.747317050981717E-5 6 0.0 0.0 0.0 0.0 1.299642273464229E-4 7 0.0 0.0 0.0 0.0 1.9494634101963434E-4 8 0.0 0.0 0.0 0.0 1.9494634101963434E-4 9 0.0 0.0 0.0 0.0 1.9494634101963434E-4 10-11 0.0 0.0 0.0 0.0 2.111918694379372E-4 12-13 0.0 0.0 0.0 4.8736585254908585E-5 5.848390230589031E-4 14-15 0.0 0.0 0.0 9.747317050981717E-5 6.335756083138116E-4 16-17 0.0 0.0 0.0 9.747317050981717E-5 7.472943072419317E-4 18-19 0.0 0.0 0.0 1.299642273464229E-4 7.635398356602345E-4 20-21 0.0 0.0 0.0 2.2743739785624007E-4 7.960308924968402E-4 22-23 0.0 0.0 0.0 7.960308924968402E-4 8.122764209151431E-4 24-25 0.0 0.0 0.0 0.0014783430860655605 8.122764209151431E-4 26-27 0.0 0.0 0.0 0.0025343024332552463 8.28521949333446E-4 28-29 0.0 0.0 0.0 0.006904349577778716 8.447674777517488E-4 30-31 0.0 0.0 0.0 0.022045182063636985 8.447674777517488E-4 32-33 0.0 0.0 0.0 0.05219688280800709 8.772585345883546E-4 34-35 0.0 0.0 0.0 0.09739194286772565 8.772585345883546E-4 36-37 0.0 0.0 0.0 0.1720888825350822 8.772585345883546E-4 38-39 0.0 0.0 0.0 0.28163248065969837 8.772585345883546E-4 40-41 0.0 0.0 0.0 0.399510034862904 8.772585345883546E-4 42-43 0.0 0.0 0.0 0.5217413906822147 8.935040630066574E-4 44-45 0.0 0.0 0.0 0.6765287854518044 9.259951198432631E-4 46-47 0.0 0.0 0.0 0.8576989183727179 9.584861766798688E-4 48-49 0.0 0.0 0.0 1.0444250220126912 0.0010397138187713832 50-51 0.0 0.0 0.0 1.2478027922814245 0.0010722048756079889 52-53 0.0 0.0 0.0 1.4475415641844582 0.0011696780461178061 54-55 0.0 0.0 0.0 1.6656702742570109 0.0011696780461178061 56-57 0.0 0.0 0.0 1.9157864297852014 0.0012021691029544118 58-59 0.0 0.0 0.0 2.1737816665962697 0.0012021691029544118 60-61 0.0 0.0 0.0 2.4617986399243605 0.0012021691029544118 62-63 0.0 0.0 0.0 2.7470863644781773 0.0012509056882093203 64-65 0.0 0.0 0.0 3.0407080451105832 0.001299642273464229 66-67 0.0 0.0 0.0 3.3859742605847742 0.001315887801882532 68-69 3.2491056836605725E-5 0.0 0.0 3.767841651585401 0.0013483788587191376 70-71 3.2491056836605725E-5 0.0 0.0 4.159342640938082 0.0013808699155557433 72-73 3.2491056836605725E-5 0.0 0.0 4.5700133538243595 0.001429606500810652 74-75 3.2491056836605725E-5 1.6245528418302863E-5 0.0 4.9874259610042335 0.0014783430860655605 76-77 3.2491056836605725E-5 3.2491056836605725E-5 0.0 5.442414475415642 0.0014945886144838634 78-79 3.2491056836605725E-5 3.2491056836605725E-5 0.0 5.947471708412259 0.0015108341429021662 80-81 3.2491056836605725E-5 3.2491056836605725E-5 0.0 6.47288458851701 0.0015433251997387719 82-83 3.2491056836605725E-5 3.2491056836605725E-5 0.0 7.0171422815869935 0.0016895349555034976 84-85 3.2491056836605725E-5 3.2491056836605725E-5 0.0 7.584029995743672 0.0016895349555034976 86-87 3.2491056836605725E-5 3.2491056836605725E-5 0.0 8.16535998466422 0.0017545170691767092 88-89 3.2491056836605725E-5 3.2491056836605725E-5 0.0 8.773430113361297 0.0018357447112682234 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7325 0.0 55.48207 1 GTATCAA 12950 0.0 41.439873 1 TATCAAC 17265 0.0 31.000307 2 ATCAACG 17440 0.0 30.56332 3 TCAACGC 17760 0.0 30.012629 4 TAAGGTG 2380 0.0 29.342327 5 CAACGCA 18245 0.0 29.214817 5 AACGCAG 18995 0.0 28.148527 6 GGTAAGG 2570 0.0 26.433645 3 AGGTAAG 2320 0.0 26.429924 2 GTAAGGT 2600 0.0 26.31136 4 GTACATG 15600 0.0 24.9243 1 ACGCAGA 21420 0.0 24.806595 7 TACATGG 15455 0.0 24.696804 2 CGCAGAG 21635 0.0 24.538132 8 TGGTATC 3240 0.0 24.206562 2 GTGGTAT 3485 0.0 23.595953 1 ACATGGG 15810 0.0 23.257511 3 AAGGTAA 2485 0.0 22.953508 1 GCAGAGT 23465 0.0 22.239944 9 >>END_MODULE