FastQCFastQC Report
Thu 26 May 2016
SRR938662_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938662_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3098694
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136160.43941092602238235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103270.3332694354460298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72580.2342277101256207No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA60500.19524354453844103No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA59630.192435910096318No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA54370.17546101680256262No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48390.15616256397049855No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47680.1538712760924441No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC46180.14903052705430095No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG42360.13670275283716302No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA40400.1303775074273226No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT40290.13002251916452545No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG38630.12466542356231367No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA37910.12234186402400496No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG36950.11924378463959333No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA35730.11530664208857022No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT34590.1116276728195814No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT33470.10801324687110117No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG31240.10081666663439501No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70800.054.4609531
GTATCAA136150.045.6973951
TATCAAC178200.034.8276982
ATCAACG178200.034.6143573
TCAACGC181600.033.9662934
CAACGCA184850.033.368575
AACGCAG191650.032.302846
ACGCAGA217800.028.3121517
CGCAGAG219200.028.0663058
TAAGGTG18750.027.8787755
GCAGAGT233050.025.541779
TGGTATC30850.025.1085572
GTACATG159450.024.9881671
TACATGG160500.024.1604182
GAGTACT133100.023.0906812-13
ACATGGG162850.023.0238823
GTGGTAT34950.022.8820841
GTAAGGT22050.022.4137274
TAAGGTA10950.022.1332574
GTACTTT139200.021.80738814-15
Produced by FastQC (version 0.11.5)