##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938662_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3098694 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.188262216275632 31.0 31.0 34.0 28.0 34.0 2 31.267043793288398 31.0 31.0 34.0 28.0 34.0 3 31.3326436879537 31.0 31.0 34.0 28.0 34.0 4 34.97772577737589 37.0 35.0 37.0 32.0 37.0 5 35.01855071846397 37.0 35.0 37.0 32.0 37.0 6 35.0632514859486 37.0 35.0 37.0 32.0 37.0 7 34.996503042894844 37.0 35.0 37.0 32.0 37.0 8 34.950729565423366 37.0 35.0 37.0 32.0 37.0 9 36.470825450980314 39.0 37.0 39.0 32.0 39.0 10-11 36.332716944622476 39.0 35.0 39.0 32.0 39.0 12-13 36.201120697945655 39.0 35.0 39.0 32.0 39.0 14-15 37.20396851060479 39.0 36.0 41.0 31.5 41.0 16-17 37.09006149042145 39.0 36.0 41.0 31.0 41.0 18-19 37.113020840392764 39.0 36.0 41.0 31.0 41.0 20-21 37.194920666577595 39.0 36.0 41.0 31.0 41.0 22-23 37.130209694794004 39.0 36.0 41.0 31.0 41.0 24-25 37.021236043313735 39.0 36.0 41.0 30.5 41.0 26-27 36.84459049522154 39.0 36.0 41.0 30.0 41.0 28-29 36.67032917738892 39.0 36.0 41.0 30.0 41.0 30-31 36.43127491775567 39.0 35.5 40.5 30.0 41.0 32-33 36.12743255707082 38.5 35.0 40.0 29.5 41.0 34-35 35.88704563922737 38.0 35.0 40.0 28.5 41.0 36-37 35.59879194267004 38.0 35.0 40.0 27.0 41.0 38-39 35.33086422860728 38.0 34.0 40.0 26.5 41.0 40-41 35.22849803820577 38.0 34.0 40.0 26.0 41.0 42-43 35.010400187950154 38.0 34.0 40.0 25.0 41.0 44-45 34.694460634060675 38.0 33.5 40.0 24.0 41.0 46-47 34.28482257363909 38.0 33.0 40.0 23.5 41.0 48-49 33.88750502631108 38.0 33.0 40.0 22.0 41.0 50-51 33.115128341165665 37.5 32.0 39.5 20.0 40.5 52-53 33.14955720054965 37.0 32.5 39.5 20.0 40.5 54-55 33.280799427113486 37.0 32.5 40.0 18.5 41.0 56-57 33.00034143416549 37.0 32.0 40.0 17.5 41.0 58-59 32.681501303452364 36.5 31.5 40.0 14.5 41.0 60-61 32.26407157337898 36.0 31.0 39.0 11.0 41.0 62-63 31.77205041866025 35.5 31.0 39.0 8.0 40.5 64-65 31.302790465918868 35.0 30.0 39.0 7.0 40.0 66-67 30.80002284833546 35.0 29.5 38.0 2.0 40.0 68-69 30.274858537177277 34.5 29.0 38.0 2.0 40.0 70-71 29.68058527237604 34.0 28.0 37.0 2.0 39.0 72-73 29.17969344504491 34.0 28.0 36.5 2.0 39.0 74-75 28.632917448447635 34.0 26.5 36.0 2.0 39.0 76-77 27.95115329232251 33.5 26.0 35.5 2.0 38.0 78-79 27.2252395686699 33.0 25.0 35.0 2.0 37.0 80-81 26.632450638882055 32.0 24.0 35.0 2.0 36.5 82-83 26.011427394896042 32.0 22.5 35.0 2.0 36.0 84-85 25.39764558875449 31.5 20.0 34.5 2.0 36.0 86-87 24.781209115840415 31.0 18.0 34.0 2.0 35.0 88-89 24.019222453072167 31.0 10.0 34.0 2.0 35.0 90-91 23.227801454419186 30.0 4.5 34.0 2.0 35.0 92-93 22.434729114910994 29.5 2.0 34.0 2.0 35.0 94-95 21.510804551853134 29.0 2.0 34.0 2.0 35.0 96-97 20.31566927873485 28.0 2.0 34.0 2.0 35.0 98-99 18.873611753855013 26.0 2.0 33.0 2.0 35.0 100-101 15.38031022101569 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5203040300866135 1101 2 0.6607512790934216 1101 3 0.5926136488630789 1101 4 0.5466976251003075 1101 5 0.4095576937222205 1101 6 0.4387501362243853 1101 7 0.3741157950628562 1101 8 0.5036537601804341 1101 9 0.3592497776673014 1101 10-11 0.5540084425689997 1101 12-13 0.5021366551415127 1101 14-15 0.6807639673886925 1101 16-17 0.6990627021964926 1101 18-19 0.5777540585927952 1101 20-21 0.7331341746779927 1101 22-23 0.740247251005691 1101 24-25 0.7512407888373716 1101 26-27 0.8104681257777102 1101 28-29 0.8766696589166116 1101 30-31 0.7016891111336321 1101 32-33 0.8660984285886428 1101 34-35 0.7308671992816684 1101 36-37 0.6870638056872309 1101 38-39 0.8223464550447659 1101 40-41 0.8048957576486444 1101 42-43 0.8872471250142908 1101 44-45 0.7397168426802025 1101 46-47 0.7508650689271619 1101 48-49 0.85253098402044 1101 50-51 0.956630853839215 1101 52-53 0.9195518819153179 1101 54-55 1.0242684719364448 1101 56-57 0.7424992807033561 1101 58-59 0.7693538888987987 1101 60-61 0.7677032424672134 1101 62-63 0.7410949500651292 1101 64-65 0.6868204047281061 1101 66-67 0.600471699702986 1101 68-69 0.7357786063099958 1101 70-71 0.9084702398110416 1101 72-73 0.9355320393343192 1101 74-75 0.889653302650018 1101 76-77 1.0330428751176193 1101 78-79 0.9692757813704844 1101 80-81 0.9602510557421695 1101 82-83 0.952111347265209 1101 84-85 1.0120634660643404 1101 86-87 1.0607825758735707 1101 88-89 1.1157194694309496 1101 90-91 1.1290345021295458 1101 92-93 1.10378280434551 1101 94-95 1.0343558050555934 1101 96-97 0.9457437396287851 1101 98-99 0.9228369634631086 1101 100-101 1.1365953916936444 1102 1 0.4511681203419933 1102 2 0.5252428980427482 1102 3 0.4943098680655176 1102 4 0.5653267468352396 1102 5 0.5338887790648883 1102 6 0.5996160943868176 1102 7 0.44896300795046784 1102 8 0.4625054229428187 1102 9 0.6155591361771897 1102 10-11 0.6349970648870595 1102 12-13 0.6553696860039437 1102 14-15 0.7135586666704299 1102 16-17 0.6683288334358863 1102 18-19 0.7792725606259197 1102 20-21 0.6674678579483455 1102 22-23 0.6496473306872943 1102 24-25 0.5519538741764833 1102 26-27 0.46693637460894166 1102 28-29 0.4600646154493546 1102 30-31 0.49190157373854504 1102 32-33 0.5165582677003684 1102 34-35 0.6381951434667172 1102 36-37 0.6084699170535046 1102 38-39 0.6009487817712653 1102 40-41 0.8342970431942973 1102 42-43 0.9313143625748239 1102 44-45 0.9660453324596148 1102 46-47 1.0141301645421308 1102 48-49 0.8356318929700208 1102 50-51 0.9488674390450385 1102 52-53 0.8676159381227748 1102 54-55 0.9588422196850459 1102 56-57 0.9354830435075385 1102 58-59 1.120753311857392 1102 60-61 1.1306774824920964 1102 62-63 0.8793971378217016 1102 64-65 0.9235957262475161 1102 66-67 0.9488559453256684 1102 68-69 0.8849629043050093 1102 70-71 0.9174888024329206 1102 72-73 1.1627420216424014 1102 74-75 0.9837245154241359 1102 76-77 1.0086048432296089 1102 78-79 1.014923203274531 1102 80-81 1.0122809121083876 1102 82-83 1.0726064253892424 1102 84-85 0.8481419873203535 1102 86-87 1.04083978643812 1102 88-89 1.0448026196680118 1102 90-91 1.1116389527136512 1102 92-93 1.2731575667932482 1102 94-95 0.8470421154840118 1102 96-97 0.9895114322847753 1102 98-99 1.3506344498276839 1102 100-101 1.0817059676533258 1103 1 0.4410997345385965 1103 2 0.49607283826975745 1103 3 0.37615609116387816 1103 4 0.4562694893063721 1103 5 0.41778737013711265 1103 6 0.41535060911307653 1103 7 0.362240293922234 1103 8 0.39401516270741155 1103 9 0.5216606551328269 1103 10-11 0.4953865301223601 1103 12-13 0.41699966673235167 1103 14-15 0.5853910304353818 1103 16-17 0.5138228926968935 1103 18-19 0.6378843793740785 1103 20-21 0.46072987650851616 1103 22-23 0.4780581006609381 1103 24-25 0.6378550699844538 1103 26-27 0.5214777471077312 1103 28-29 0.5953547457125978 1103 30-31 0.673968590739527 1103 32-33 0.7032679037323533 1103 34-35 0.6957002350412367 1103 36-37 0.7223126905692112 1103 38-39 0.533440276547239 1103 40-41 0.6266762621886102 1103 42-43 0.6727569449484463 1103 44-45 0.5947008517709662 1103 46-47 0.5267662387254362 1103 48-49 0.5578091523261861 1103 50-51 0.6892838374317236 1103 52-53 0.6303727295930841 1103 54-55 0.5356625646461026 1103 56-57 0.46507552673768515 1103 58-59 0.38206671872555376 1103 60-61 0.4485988359630326 1103 62-63 0.5395035668058874 1103 64-65 0.5107738720940596 1103 66-67 0.36653448539231803 1103 68-69 0.5360848612857865 1103 70-71 0.6094581885010832 1103 72-73 0.7658134844657276 1103 74-75 0.637926486761458 1103 76-77 0.590535564946979 1103 78-79 0.42048683914690343 1103 80-81 0.5059703959091522 1103 82-83 0.6831144491368768 1103 84-85 0.5217457023069443 1103 86-87 0.5965303930802648 1103 88-89 0.6140772811268818 1103 90-91 0.7267854172187285 1103 92-93 0.8485153070937699 1103 94-95 0.5364388688818842 1103 96-97 0.5264897104154542 1103 98-99 0.8258779004186927 1103 100-101 0.6439022855079646 1104 1 0.49211103436481807 1104 2 0.5279365692066662 1104 3 0.5102257687144025 1104 4 0.4497369434054477 1104 5 0.408941333089615 1104 6 0.3957807198188519 1104 7 0.42211708061503117 1104 8 0.35727471380040043 1104 9 0.4593726095362314 1104 10-11 0.4263985405153079 1104 12-13 0.404790885631769 1104 14-15 0.5463303477778538 1104 16-17 0.4883939399036805 1104 18-19 0.6501962445157758 1104 20-21 0.5901603832126057 1104 22-23 0.6010910432085055 1104 24-25 0.6625170515786962 1104 26-27 0.5884987765114502 1104 28-29 0.6923981916811357 1104 30-31 0.6982611554367537 1104 32-33 0.639254464685358 1104 34-35 0.650197837137874 1104 36-37 0.6775081521743616 1104 38-39 0.7539992150746571 1104 40-41 0.7611951008943763 1104 42-43 0.7482921974861938 1104 44-45 0.8448210516528718 1104 46-47 0.9916001230073093 1104 48-49 0.9253102421643788 1104 50-51 0.796998815633664 1104 52-53 0.758526290841985 1104 54-55 0.8651037946837832 1104 56-57 0.8740953025357285 1104 58-59 0.7648173095462241 1104 60-61 0.9371842558081269 1104 62-63 0.9231289456559431 1104 64-65 0.920451764936967 1104 66-67 0.8557923503156601 1104 68-69 0.8113779895091078 1104 70-71 0.7608870889094526 1104 72-73 0.9624553046368476 1104 74-75 1.0047188900251918 1104 76-77 1.14544765565471 1104 78-79 1.1075948118352095 1104 80-81 1.0859854604423589 1104 82-83 0.8167024195015777 1104 84-85 0.8335817989409051 1104 86-87 0.8470544360389525 1104 88-89 0.9031690186622043 1104 90-91 0.9182137133860486 1104 92-93 0.8998726840341185 1104 94-95 0.6275349491728157 1104 96-97 0.5676133705498358 1104 98-99 0.5812505887752799 1104 100-101 0.5718145134017565 1105 1 0.48294481936740397 1105 2 0.4987835446133033 1105 3 0.43943655474235754 1105 4 0.40826385351531513 1105 5 0.42150949003927707 1105 6 0.4051217860146892 1105 7 0.2999882073286173 1105 8 0.3684934420849615 1105 9 0.5816192373296616 1105 10-11 0.5505609344264499 1105 12-13 0.5024826124042789 1105 14-15 0.6955763509500983 1105 16-17 0.6320673772170906 1105 18-19 0.6531543516880518 1105 20-21 0.6792952033390307 1105 22-23 0.6114446557197297 1105 24-25 0.6712143852225623 1105 26-27 0.7675107331046789 1105 28-29 0.6856921199881043 1105 30-31 0.7301975547081057 1105 32-33 0.8074369238695382 1105 34-35 0.7420569766325755 1105 36-37 0.6878499536236475 1105 38-39 0.7416126161073393 1105 40-41 0.6270354443007022 1105 42-43 0.7271844053428396 1105 44-45 0.5982407992261471 1105 46-47 0.34746011907108 1105 48-49 0.4706078607456661 1105 50-51 0.31424860451834746 1105 52-53 0.3325165993611634 1105 54-55 0.26296202775365884 1105 56-57 0.4246260524871204 1105 58-59 0.4608408751669444 1105 60-61 0.4553376799596194 1105 62-63 0.5835837059649798 1105 64-65 0.5418795715717195 1105 66-67 0.4651313273186304 1105 68-69 0.36973769296717407 1105 70-71 0.5762855324132836 1105 72-73 0.4202083824571474 1105 74-75 0.51944740297332 1105 76-77 0.41183594924233446 1105 78-79 0.70201665501137 1105 80-81 0.5981085362185681 1105 82-83 0.8101542243149318 1105 84-85 0.6251158434070021 1105 86-87 0.6868833786248452 1105 88-89 0.6216830422506163 1105 90-91 0.5991248175173602 1105 92-93 0.6847539549661086 1105 94-95 0.7702746597198917 1105 96-97 0.771120816741842 1105 98-99 1.0497758892570062 1105 100-101 0.6929986204368053 1106 1 0.2930758727108298 1106 2 0.3650535056226474 1106 3 0.13617789310137596 1106 4 0.27336179092684887 1106 5 0.38135186415969 1106 6 0.07917504459204139 1106 7 0.2030809943094809 1106 8 0.3181164312339959 1106 9 0.47162815477836517 1106 10-11 0.20109971802351367 1106 12-13 0.1678293618059854 1106 14-15 0.5248108607460935 1106 16-17 0.23925511058369153 1106 18-19 0.024107911840367535 1106 20-21 0.3763916101185458 1106 22-23 0.39197966892732694 1106 24-25 0.45868899409382635 1106 26-27 0.2865739008948722 1106 28-29 0.2927115063080521 1106 30-31 0.30096647253890296 1106 32-33 0.5103258513426425 1106 34-35 0.3507193761848484 1106 36-37 0.36764549849549866 1106 38-39 0.1824868407472522 1106 40-41 0.371389384413888 1106 42-43 -0.012556454834147246 1106 44-45 0.394588466220398 1106 46-47 0.2544005014031754 1106 48-49 0.4496061733418557 1106 50-51 0.19382388222634717 1106 52-53 0.5530512866632691 1106 54-55 0.475874740709024 1106 56-57 -0.1291315536133837 1106 58-59 -0.3501693363513496 1106 60-61 0.5050220292471508 1106 62-63 0.41885421033745374 1106 64-65 0.6436683432282813 1106 66-67 0.022479288189742874 1106 68-69 0.021382363822286976 1106 70-71 0.14300788396754527 1106 72-73 0.6254905800941089 1106 74-75 0.6405777668580477 1106 76-77 0.6727161638005335 1106 78-79 0.8649838447563631 1106 80-81 0.4995253822737382 1106 82-83 0.456351834303824 1106 84-85 0.49901761211861384 1106 86-87 0.4889948529145549 1106 88-89 0.15606264461219155 1106 90-91 0.3530298677601884 1106 92-93 0.3181462884326116 1106 94-95 0.11366695385297376 1106 96-97 0.06670507820345861 1106 98-99 0.017559344158708967 1106 100-101 -0.2668882004505928 1107 1 0.39923636382862426 1107 2 0.3884289098993676 1107 3 0.3161158359235543 1107 4 0.4067899560858734 1107 5 0.40431152485825095 1107 6 0.36365226012460283 1107 7 0.3428784835087413 1107 8 0.2866828847960363 1107 9 0.41406683847417725 1107 10-11 0.4118846489473924 1107 12-13 0.45652846245213397 1107 14-15 0.49102915869636377 1107 16-17 0.5610632267142321 1107 18-19 0.5092457463748516 1107 20-21 0.5251601045939012 1107 22-23 0.5749395752488695 1107 24-25 0.4717743443388329 1107 26-27 0.5356773503491326 1107 28-29 0.5013318580935504 1107 30-31 0.448419922980996 1107 32-33 0.5464597137264988 1107 34-35 0.5997022567976629 1107 36-37 0.6825577496887618 1107 38-39 0.8156874842794366 1107 40-41 0.6110851536649875 1107 42-43 0.48047345937402497 1107 44-45 0.6346812369489783 1107 46-47 0.7194603132666586 1107 48-49 0.7039800510184122 1107 50-51 0.761254009165377 1107 52-53 0.6793593224323828 1107 54-55 0.6737792029842709 1107 56-57 0.7632643329493902 1107 58-59 0.7312413297728142 1107 60-61 0.6840605114253364 1107 62-63 0.851838652258035 1107 64-65 0.6811017113226008 1107 66-67 0.6503920429101093 1107 68-69 0.8262931145795811 1107 70-71 0.7889034974082456 1107 72-73 0.8221615344835236 1107 74-75 0.8549243910639746 1107 76-77 0.9240674372221527 1107 78-79 0.9485341539476231 1107 80-81 0.9163878264679326 1107 82-83 0.859435310338764 1107 84-85 0.9160863262129411 1107 86-87 0.8146510800709486 1107 88-89 0.916009280351723 1107 90-91 1.0672014589004597 1107 92-93 1.020223671415959 1107 94-95 0.9140470287039015 1107 96-97 1.0329766656593868 1107 98-99 0.7788198492129368 1107 100-101 0.3032235953790501 1108 1 0.20728171950820595 1108 2 0.2825193133253521 1108 3 0.23779326743749962 1108 4 0.24764912120843974 1108 5 0.26587870367576016 1108 6 0.23431095916801326 1108 7 0.2615828418278383 1108 8 0.2846985688857657 1108 9 0.22340839177037708 1108 10-11 0.28329455118748115 1108 12-13 0.3059915704994225 1108 14-15 0.41720962015848784 1108 16-17 0.3259226784788254 1108 18-19 0.3534854737376705 1108 20-21 0.3861746005189062 1108 22-23 0.3104972724054491 1108 24-25 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-0.9099219268494512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8600.0 3 2610.0 4 2735.0 5 2979.0 6 3677.0 7 4709.0 8 6054.0 9 8145.0 10 10344.0 11 13641.0 12 17543.0 13 21603.0 14 25014.0 15 30732.0 16 36875.0 17 42876.0 18 38719.0 19 33975.0 20 34973.0 21 37240.0 22 41062.0 23 46107.0 24 51081.0 25 57634.0 26 66010.0 27 75324.0 28 88003.0 29 101815.0 30 118886.0 31 139365.0 32 166796.0 33 202786.0 34 252539.0 35 317949.0 36 384466.0 37 392461.0 38 200741.0 39 12625.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.04091487824924 18.524273527154264 14.586974669555811 22.847836925040692 2 15.650685340623314 20.2799027127239 39.879550009217944 24.189861937434838 3 19.096035019271834 24.504094901761746 29.947831410024193 26.452038668942222 4 12.753429646716038 16.572955708414806 37.00460299788965 33.669011646979506 5 13.450267282980914 37.88340294584432 33.82454418201398 14.841785589160791 6 31.909327782623308 36.242633883377614 17.379207635283215 14.468830698715859 7 28.814415100796264 33.335162715470275 20.174297073365217 17.676125110368236 8 27.178904598784293 33.779352748865826 19.859033846275032 19.182708806074853 9 27.39097756983518 16.329183234133005 20.058913243082344 36.22092595294947 10-11 25.002017810975342 26.43963548971142 27.151147924592628 21.407198774720605 12-13 26.52942432696419 24.101347742310082 27.276442862960888 22.09278506776484 14-15 22.887026296832005 25.52313371294313 26.062364282394956 25.52747570782991 16-17 21.866795837626164 28.65070850066832 27.178666389472074 22.303829272233447 18-19 22.078454351454575 26.500531078589944 29.92367964822904 21.49733492172644 20-21 22.701812887909227 25.637613557737232 30.1422762980783 21.518297256275236 22-23 22.438525135985266 25.84568346165736 30.090942037466473 21.624849364890903 24-25 21.989337322970222 26.07723506021511 30.193534095039237 21.73989352177543 26-27 21.352623750020584 26.414566160965354 29.921644117448583 22.31116597156548 28-29 21.59007829062693 26.94544515919073 29.756524044031558 21.707952506150786 30-31 21.59140258504926 26.365988110878604 29.529042654830285 22.51356664924186 32-33 21.159803101099563 26.508931869312647 29.548266447631814 22.782998581955972 34-35 22.456724117083844 26.534760427030363 29.30003562837426 21.70847982751153 36-37 22.60784933492224 25.84328902494974 29.204208688386394 22.344652951741626 38-39 21.79909986902344 26.33983051326188 29.17491221826522 22.686157399449463 40-41 22.4891899922513 26.165504523248433 29.38035103655188 21.964954447948386 42-43 22.056324307469634 26.263830191060556 29.029432641397435 22.650412860072375 44-45 21.94251761257988 26.57748349115631 29.337227392085985 22.14277150417782 46-47 22.684330183460357 26.91916538156411 28.18872190983131 22.207782525144225 48-49 22.12307092981542 27.024774303367455 28.036860891993694 22.815293874823432 50-51 22.490522631755265 27.312022783122053 27.975874264225087 22.221580320897587 52-53 22.931123683154805 26.66634714784265 28.143831237257572 22.25869793174497 54-55 22.03797289411716 27.155650974964885 28.456577785466415 22.34979834545154 56-57 22.27931924867098 27.343417044021816 27.917288419826885 22.45997528748032 58-59 22.20855375154335 26.628472408812176 27.94558367193863 23.217390167705844 60-61 21.481961304132145 27.063191674840326 28.572163981865284 22.882683039162245 62-63 21.890537389134483 26.982137589742894 28.658473220261964 22.468851800860655 64-65 21.558443779889636 27.121151583259156 28.289062192389693 23.031342444461515 66-67 21.55860229411722 27.05018673891487 28.410101086708334 22.98110988025957 68-69 22.43381521755794 26.477092008768967 28.484303731489604 22.604789042183494 70-71 22.04972437662959 26.724080468628326 27.95509328263359 23.271101872108495 72-73 21.968368560162176 26.91633889735558 27.98330325295743 23.131989289524814 74-75 22.01031447779224 27.350731785650595 27.796363706788657 22.84259002976851 76-77 22.199510960147386 26.7612148018472 28.138834127958546 22.900440110046876 78-79 22.32308991422947 27.10174747386567 27.82000081650871 22.755161795396152 80-81 22.594398491909114 27.15812588531112 27.520754484366076 22.726721138413684 82-83 22.4291555074754 27.133048112015718 27.689473345340105 22.74832303516878 84-85 22.336354975381923 27.253338350756618 27.723979969895463 22.686326703966003 86-87 22.662708124424913 27.31398987944606 27.150182010536245 22.873119985592783 88-89 22.49401184073756 27.231097753875353 27.079634447894946 23.19525595749214 90-91 22.401803847558156 27.179727150139737 27.1618262003402 23.256642801961906 92-93 22.726888316406882 26.699646967037882 27.503259349305086 23.07020536725015 94-95 22.405950037261174 26.48627857476858 27.74955907207491 23.35821231589534 96-97 22.85139374006192 25.928305890476828 27.708205513364543 23.512094856096706 98-99 22.5382713562746 26.253166718392894 27.282591275810862 23.92597064952164 100-101 23.223743389320553 25.615978975066817 27.55632324736219 23.60395438825044 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 752.0 1 514.5 2 409.5 3 584.0 4 543.0 5 528.0 6 899.5 7 1883.5 8 3104.5 9 3569.5 10 3099.0 11 2659.5 12 2720.0 13 2932.5 14 3229.5 15 3574.0 16 3882.5 17 4157.5 18 4618.5 19 5248.0 20 6015.0 21 7038.0 22 8337.0 23 10035.5 24 12244.5 25 15269.0 26 19134.0 27 23698.5 28 28197.0 29 32274.0 30 37089.0 31 45469.5 32 59203.0 33 64518.5 34 62156.0 35 64885.5 36 70715.0 37 76197.5 38 80145.5 39 83118.0 40 86318.5 41 91354.0 42 95738.5 43 99185.5 44 101095.0 45 106021.0 46 109178.5 47 110303.0 48 112225.5 49 110029.0 50 108274.0 51 105599.5 52 101951.5 53 103010.5 54 105625.5 55 101427.5 56 93601.0 57 86589.5 58 82495.5 59 73812.5 60 58388.5 61 47221.0 62 40164.5 63 30139.5 64 20361.5 65 15588.0 66 12121.0 67 9283.0 68 7070.0 69 5138.5 70 3758.5 71 2701.5 72 2050.5 73 1777.0 74 1406.5 75 1066.5 76 762.5 77 517.0 78 368.5 79 251.5 80 157.5 81 107.0 82 66.0 83 28.5 84 14.0 85 14.0 86 14.0 87 11.0 88 9.5 89 6.5 90 5.0 91 4.0 92 3.5 93 2.5 94 2.0 95 2.0 96 1.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18511024321859465 2 0.04760069887507447 3 0.04608393084312294 4 0.05105376652228326 5 0.05773400019492082 6 0.06173568606645251 7 0.03578927122200514 8 0.0345629481323422 9 0.04059774859989402 10-11 0.041243181804979774 12-13 0.03809669493018671 14-15 0.03354639083433214 16-17 0.031965079481872036 18-19 0.03950051215124824 20-21 0.04779432883660019 22-23 0.044906015243841434 24-25 0.06468854297971983 26-27 0.04979517177236604 28-29 0.04274381400680416 30-31 0.05089240822101182 32-33 0.04743934057380303 34-35 0.046584141577064406 36-37 0.04476079277269714 38-39 0.02618845229635453 40-41 0.024348967661860125 42-43 0.021654284030627094 44-45 0.004114636682421691 46-47 0.011875970973577902 48-49 0.011053043637093562 50-51 0.00839063166611482 52-53 0.009197423172472016 54-55 0.010520561242897814 56-57 0.008535854137259116 58-59 0.010407610432007807 60-61 0.010552832903152102 62-63 0.003872599230514533 64-65 0.006841591973909008 66-67 0.0037596484196245256 68-69 0.008600397457767693 70-71 0.02312264457219719 72-73 0.01954049028397125 74-75 0.02005683684803985 76-77 0.012198687576120778 78-79 0.0043728099644559934 80-81 0.00668023367263757 82-83 0.005308688111830339 84-85 0.005098922320177468 86-87 0.00797110008280908 88-89 0.029496297472419025 90-91 0.035272924657936536 92-93 0.02204154395367855 94-95 0.03115828797551485 96-97 0.0347081706034865 98-99 0.021057258315922773 100-101 0.11946968626137333 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3098694.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.97025693636123 #Duplication Level Percentage of deduplicated Percentage of total 1 72.76509373245284 33.450300548792654 2 13.726408508045113 12.620130518565773 3 5.3947023451745 7.439875587085868 4 2.596629473551219 4.774708962707117 5 1.4592584316985517 3.354124252086698 6 0.8999395140600126 2.4822270413113707 7 0.6147928025028536 1.9783528165577238 8 0.42335606069758314 1.5569429508666908 9 0.3157904469571677 1.3065271186202416 >10 1.6065826249941975 13.03490699035195 >50 0.11740719084366077 3.742492919974976 >100 0.06838551993549054 6.013444058608833 >500 0.006497624945527222 1.9606892732047576 >1k 0.004731965992630214 4.705719189684895 >5k 2.825054323958336E-4 0.8021975585335411 >10k+ 1.412527161979168E-4 0.7773602130471335 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13616 0.43941092602238235 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10327 0.3332694354460298 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7258 0.2342277101256207 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6050 0.19524354453844103 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5963 0.192435910096318 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5437 0.17546101680256262 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4839 0.15616256397049855 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4768 0.1538712760924441 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4618 0.14903052705430095 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4236 0.13670275283716302 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4040 0.1303775074273226 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4029 0.13002251916452545 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3863 0.12466542356231367 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3791 0.12234186402400496 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3695 0.11924378463959333 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3573 0.11530664208857022 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3459 0.1116276728195814 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3347 0.10801324687110117 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3124 0.10081666663439501 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.454332050857555E-5 2 0.0 0.0 0.0 0.0 6.454332050857555E-5 3 0.0 0.0 0.0 0.0 6.454332050857555E-5 4 0.0 0.0 0.0 0.0 6.454332050857555E-5 5 0.0 0.0 0.0 0.0 6.454332050857555E-5 6 0.0 0.0 0.0 0.0 1.290866410171511E-4 7 0.0 0.0 0.0 0.0 1.290866410171511E-4 8 0.0 0.0 0.0 0.0 1.290866410171511E-4 9 0.0 0.0 0.0 0.0 1.290866410171511E-4 10-11 0.0 0.0 0.0 0.0 1.290866410171511E-4 12-13 0.0 0.0 0.0 0.0 2.581732820343022E-4 14-15 0.0 0.0 0.0 0.0 2.9044494228859E-4 16-17 0.0 0.0 0.0 3.227166025428778E-5 3.2271660254287775E-4 18-19 0.0 0.0 0.0 6.454332050857555E-5 3.2271660254287775E-4 20-21 0.0 0.0 0.0 1.7749413139858276E-4 4.0339575317859715E-4 22-23 0.0 0.0 0.0 5.486182243228921E-4 4.1953158330574106E-4 24-25 0.0 0.0 0.0 0.001129508108900072 4.1953158330574106E-4 26-27 0.0 0.0 0.0 0.0027914986119958922 4.5180324356002883E-4 28-29 0.0 0.0 0.0 0.007019086105307591 4.5180324356002883E-4 30-31 0.0 0.0 0.0 0.022138358934441413 4.679390736871727E-4 32-33 0.0 0.0 0.0 0.052715757025379076 4.840749038143166E-4 34-35 0.0 0.0 0.0 0.09678270910260903 5.163465640686044E-4 36-37 0.0 0.0 0.0 0.17263724653031245 5.163465640686044E-4 38-39 0.0 0.0 0.0 0.28689505966061835 5.163465640686044E-4 40-41 1.613583012714389E-5 0.0 0.0 0.40604202931944877 5.163465640686044E-4 42-43 3.227166025428778E-5 0.0 0.0 0.5289002399075223 5.163465640686044E-4 44-45 3.227166025428778E-5 0.0 0.0 0.6823197127564065 5.324823941957482E-4 46-47 3.227166025428778E-5 0.0 0.0 0.8620083170522808 5.64754054450036E-4 48-49 3.227166025428778E-5 0.0 0.0 1.0533631265300802 6.131615448314677E-4 50-51 3.227166025428778E-5 0.0 0.0 1.260256740420319 6.131615448314677E-4 52-53 3.227166025428778E-5 0.0 0.0 1.4560650390132102 6.454332050857555E-4 54-55 3.227166025428778E-5 0.0 0.0 1.6726724226399896 7.09976525594331E-4 56-57 3.227166025428778E-5 3.227166025428778E-5 0.0 1.9223098505370326 7.09976525594331E-4 58-59 3.227166025428778E-5 3.227166025428778E-5 0.0 2.1871633662439725 7.09976525594331E-4 60-61 3.227166025428778E-5 3.227166025428778E-5 0.0 2.48123887031117 7.09976525594331E-4 62-63 3.227166025428778E-5 3.227166025428778E-5 0.0 2.766649433600091 7.422481858486188E-4 64-65 3.227166025428778E-5 3.227166025428778E-5 0.0 3.064742759368947 7.745198461029066E-4 66-67 3.227166025428778E-5 3.227166025428778E-5 0.0 3.409597720846266 7.745198461029066E-4 68-69 3.227166025428778E-5 4.840749038143167E-5 0.0 3.790984201731439 7.745198461029066E-4 70-71 3.227166025428778E-5 6.454332050857555E-5 0.0 4.184827543474767 7.745198461029066E-4 72-73 3.227166025428778E-5 6.454332050857555E-5 0.0 4.591950673412734 8.390631666114821E-4 74-75 3.227166025428778E-5 6.454332050857555E-5 0.0 5.002155746904986 9.036064871200577E-4 76-77 3.227166025428778E-5 6.454332050857555E-5 0.0 5.448424400731405 9.036064871200577E-4 78-79 3.227166025428778E-5 6.454332050857555E-5 0.0 5.9391472665581055 9.197423172472016E-4 80-81 3.227166025428778E-5 6.454332050857555E-5 0.0 6.4471516064509755 9.842856377557771E-4 82-83 3.227166025428778E-5 6.454332050857555E-5 0.0 6.978972431611512 0.0010649647883914965 84-85 3.227166025428778E-5 6.454332050857555E-5 0.0 7.524460304889738 0.0010649647883914965 86-87 4.840749038143167E-5 6.454332050857555E-5 0.0 8.08668103400981 0.0010972364486457843 88-89 6.454332050857555E-5 6.454332050857555E-5 0.0 8.667506375266484 0.001129508108900072 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7080 0.0 54.460953 1 GTATCAA 13615 0.0 45.697395 1 TATCAAC 17820 0.0 34.827698 2 ATCAACG 17820 0.0 34.614357 3 TCAACGC 18160 0.0 33.966293 4 CAACGCA 18485 0.0 33.36857 5 AACGCAG 19165 0.0 32.30284 6 ACGCAGA 21780 0.0 28.312151 7 CGCAGAG 21920 0.0 28.066305 8 TAAGGTG 1875 0.0 27.878775 5 GCAGAGT 23305 0.0 25.54177 9 TGGTATC 3085 0.0 25.108557 2 GTACATG 15945 0.0 24.988167 1 TACATGG 16050 0.0 24.160418 2 GAGTACT 13310 0.0 23.09068 12-13 ACATGGG 16285 0.0 23.023882 3 GTGGTAT 3495 0.0 22.882084 1 GTAAGGT 2205 0.0 22.413727 4 TAAGGTA 1095 0.0 22.133257 4 GTACTTT 13920 0.0 21.807388 14-15 >>END_MODULE