FastQCFastQC Report
Thu 26 May 2016
SRR938662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938662_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3098694
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133540.4309557510357589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108030.34863074572707087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74520.24048841221495248No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA61790.19940658871124414No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA59980.19356541820521805No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC53570.1728792839822196No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA52550.16958757463628227No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48710.15719525709863574No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46170.14899825539404665No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA45910.14815919222743518No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT38290.12356818711366788No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG38200.1232777421713793No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG37960.1225032223252764No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA37950.12247095066502209No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36180.11675886680001318No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA35970.11608116193467313No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT35330.1140157756783987No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT34800.11230537768492145No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA34380.11094996795424138No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71950.054.271761
GTATCAA133150.042.1504631
ATCAACG176650.031.5651153
TATCAAC176800.031.555662
TCAACGC178450.031.2462184
CAACGCA183750.030.3449655
AACGCAG190550.029.312296
ACGCAGA214450.026.0012057
TAAGGTG22050.025.8695355
CGCAGAG215950.025.7766118
TACATGG152750.024.5984332
GTACATG155950.024.4291671
TAAGGTA12350.024.2487624
ACATGGG154250.023.5754093
ATGGGCG5100.023.3016035
GTGTAAG12900.023.228031
GCAGAGT235750.023.1281879
CGTATAG1252.237206E-422.8298321
TGGTATC32350.021.9065272
AGGTAAG22700.021.790592
Produced by FastQC (version 0.11.5)