##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938662_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3098694 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.813041881515243 31.0 31.0 34.0 30.0 34.0 2 32.0365625002017 31.0 31.0 34.0 30.0 34.0 3 32.09377466765031 33.0 31.0 34.0 30.0 34.0 4 35.7669301970443 37.0 35.0 37.0 35.0 37.0 5 35.63886689037381 37.0 35.0 37.0 33.0 37.0 6 35.67313713454765 37.0 35.0 37.0 33.0 37.0 7 35.612615508339964 37.0 35.0 37.0 33.0 37.0 8 35.597349399456675 37.0 35.0 37.0 33.0 37.0 9 37.182507533819084 39.0 37.0 39.0 33.0 39.0 10-11 37.09590169277767 39.0 37.0 39.0 33.0 39.0 12-13 36.99712491778794 39.0 37.0 39.0 33.0 39.0 14-15 38.04527100772132 40.0 37.0 41.0 33.0 41.0 16-17 37.98529283627231 40.0 37.0 41.0 33.0 41.0 18-19 38.113876039389496 40.0 37.5 41.0 33.0 41.0 20-21 38.16482105041673 40.0 37.0 41.0 33.0 41.0 22-23 38.07489542368494 40.0 37.0 41.0 33.0 41.0 24-25 37.96693784542778 40.0 37.0 41.0 33.0 41.0 26-27 37.77824125260513 40.0 37.0 41.0 32.0 41.0 28-29 37.65619531970566 40.0 37.0 41.0 32.0 41.0 30-31 37.402168300580826 39.0 36.0 41.0 32.0 41.0 32-33 37.15469710787835 39.0 36.0 41.0 31.0 41.0 34-35 36.98791200421856 39.0 35.5 41.0 31.0 41.0 36-37 36.948282728142885 39.0 35.5 41.0 31.0 41.0 38-39 36.800509182255496 39.0 35.0 41.0 30.0 41.0 40-41 36.60853023886837 39.0 35.0 41.0 30.0 41.0 42-43 36.36286512963203 39.0 35.0 41.0 30.0 41.0 44-45 36.05927577876357 39.0 35.0 40.0 29.5 41.0 46-47 35.63734915419206 38.0 35.0 40.0 28.0 41.0 48-49 35.23032219380165 38.0 34.5 40.0 26.5 41.0 50-51 34.84977558287459 38.0 34.0 40.0 26.0 41.0 52-53 34.371395658945346 38.0 33.0 40.0 24.0 41.0 54-55 34.060130332327105 38.0 33.0 40.0 23.5 41.0 56-57 33.72134486335211 37.0 33.0 40.0 23.0 41.0 58-59 33.28441901652761 36.5 32.0 39.5 22.0 41.0 60-61 32.92588264604379 36.0 32.0 39.0 20.5 40.5 62-63 32.486939336378484 35.5 31.0 39.0 19.5 40.0 64-65 32.00483961953003 35.0 31.0 38.5 18.0 40.0 66-67 31.53989551727276 35.0 30.5 38.0 15.0 40.0 68-69 31.02062159090249 35.0 30.0 37.5 12.0 40.0 70-71 30.463946907955417 34.0 29.0 37.0 8.0 39.0 72-73 30.06768222354321 34.0 29.0 36.0 7.0 39.0 74-75 29.606315112108522 34.0 29.0 36.0 6.5 39.0 76-77 28.324464919737153 32.5 27.0 34.5 2.0 36.5 78-79 29.037385911613086 33.5 28.5 35.0 2.0 37.0 80-81 28.807648964370152 34.0 28.0 35.0 2.0 37.0 82-83 28.385298774257798 33.0 27.5 35.0 2.0 36.0 84-85 27.91220123703728 33.0 26.5 35.0 2.0 36.0 86-87 27.401554493602788 33.0 26.0 35.0 2.0 35.5 88-89 26.79654428607665 32.0 25.0 35.0 2.0 35.0 90-91 26.313941938119736 32.0 24.5 34.0 2.0 35.0 92-93 25.650750767904157 32.0 23.0 34.0 2.0 35.0 94-95 24.95947050596154 31.0 19.5 34.0 2.0 35.0 96-97 24.065854518064707 31.0 9.5 34.0 2.0 35.0 98-99 22.92308630668275 31.0 2.0 34.0 2.0 35.0 100-101 19.306563184360897 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30944216421618265 1101 2 0.3157805359322481 1101 3 0.3038360378044942 1101 4 0.21567869361661707 1101 5 0.1957480469307029 1101 6 0.21493754862949288 1101 7 0.23348809906348578 1101 8 0.23577070753673723 1101 9 0.2293374406082691 1101 10-11 0.4022912816843842 1101 12-13 0.38076415157881627 1101 14-15 0.39509798476427704 1101 16-17 0.4913422877060114 1101 18-19 0.5100655178452627 1101 20-21 0.47031782908265996 1101 22-23 0.47677669108679765 1101 24-25 0.4207496624013132 1101 26-27 0.4935364328167182 1101 28-29 0.4917236354891372 1101 30-31 0.4278595548901123 1101 32-33 0.4607250758836088 1101 34-35 0.33227498706273195 1101 36-37 0.4278649563498149 1101 38-39 0.47496549332731064 1101 40-41 0.5840815614773476 1101 42-43 0.6865647282469851 1101 44-45 0.8008128286134522 1101 46-47 0.6844395763973026 1101 48-49 0.7031591626219864 1101 50-51 0.6788987103982649 1101 52-53 0.8102000535414859 1101 54-55 0.5703158209715511 1101 56-57 0.6906906002603108 1101 58-59 0.6457180359932622 1101 60-61 0.5044792006818781 1101 62-63 0.5620466524727163 1101 64-65 0.5736903779577815 1101 66-67 0.5305671666512488 1101 68-69 0.41594734713955717 1101 70-71 0.32787923462651136 1101 72-73 0.3152292920524715 1101 74-75 0.5006435954761521 1101 76-77 0.4846004144250351 1101 78-79 0.41090451331275446 1101 80-81 0.48306637265744357 1101 82-83 0.34882345979274376 1101 84-85 0.5081961286127097 1101 86-87 0.4531278529889775 1101 88-89 0.4920868188559808 1101 90-91 0.3495974235032264 1101 92-93 0.33003345117224825 1101 94-95 0.3352645278133579 1101 96-97 0.5283018577136289 1101 98-99 0.2730585213452912 1101 100-101 0.17715880588391641 1102 1 0.3450021491546984 1102 2 0.30816186915681243 1102 3 0.33844832591378804 1102 4 0.2992709330539256 1102 5 0.17327656354650856 1102 6 0.24426531489047676 1102 7 0.351727649867307 1102 8 0.3361673126747817 1102 9 0.34218237535861107 1102 10-11 0.39785832742978045 1102 12-13 0.4334191065864843 1102 14-15 0.49639558905525405 1102 16-17 0.579967975092849 1102 18-19 0.5936310095629267 1102 20-21 0.5745542836532067 1102 22-23 0.5347722494875669 1102 24-25 0.4337215675423707 1102 26-27 0.4495206218110823 1102 28-29 0.4549429348703029 1102 30-31 0.30724189536582713 1102 32-33 0.4812656251310372 1102 34-35 0.4970321473238357 1102 36-37 0.5458027455872383 1102 38-39 0.6437354765338767 1102 40-41 0.5727750343835325 1102 42-43 0.6590719885534284 1102 44-45 0.7219580388800821 1102 46-47 0.6416972376239869 1102 48-49 0.9042374356118685 1102 50-51 0.97387817819844 1102 52-53 0.8699055448926671 1102 54-55 0.7381047096437001 1102 56-57 0.8059589375215666 1102 58-59 0.8684915912765092 1102 60-61 0.9759146310955416 1102 62-63 1.0146195466587429 1102 64-65 0.9432132281947858 1102 66-67 0.9457375126810312 1102 68-69 1.0771953100273208 1102 70-71 0.9349587796847452 1102 72-73 0.8177506710171194 1102 74-75 0.6852817741958432 1102 76-77 0.7861615206988972 1102 78-79 0.9319616925484802 1102 80-81 0.8647632775472793 1102 82-83 0.8422058375083772 1102 84-85 0.733876983688539 1102 86-87 0.6180661884118877 1102 88-89 0.7265143999222587 1102 90-91 0.5914701806108802 1102 92-93 0.7873636711301302 1102 94-95 0.8745225697147703 1102 96-97 0.7449445696545816 1102 98-99 0.5384527206663421 1102 100-101 0.41075137043163323 1103 1 0.30487512406736883 1103 2 0.2561757894930139 1103 3 0.2751249187656768 1103 4 0.2049849867745479 1103 5 0.19487712365800292 1103 6 0.15547783734347576 1103 7 0.29353279496551465 1103 8 0.16932508888059772 1103 9 0.3020872444261471 1103 10-11 0.31144278614628007 1103 12-13 0.3482975766252707 1103 14-15 0.5213452443407292 1103 16-17 0.44748097892473737 1103 18-19 0.38145409978898925 1103 20-21 0.3972926294384891 1103 22-23 0.48688812025579153 1103 24-25 0.4898180238232186 1103 26-27 0.509398833493286 1103 28-29 0.5220793648335871 1103 30-31 0.5761557907577313 1103 32-33 0.6678203864896446 1103 34-35 0.591547620097586 1103 36-37 0.7079154422603224 1103 38-39 0.6973404282894435 1103 40-41 0.6351069372152764 1103 42-43 0.7042663541793885 1103 44-45 0.740920576340045 1103 46-47 0.8785539558491564 1103 48-49 0.9406416885929687 1103 50-51 0.9747715627239799 1103 52-53 1.0414691481745066 1103 54-55 0.9635967326927286 1103 56-57 0.7528170777525034 1103 58-59 0.7790282083428863 1103 60-61 0.798694620340676 1103 62-63 0.7513531450896025 1103 64-65 0.637917015757985 1103 66-67 0.740792985529982 1103 68-69 0.5319748998294855 1103 70-71 0.4183452676215822 1103 72-73 0.5506013998264123 1103 74-75 0.6929499507513022 1103 76-77 0.7174226531865209 1103 78-79 0.7137877067303791 1103 80-81 0.7630590154714803 1103 82-83 0.8703218581373484 1103 84-85 0.6955175772317972 1103 86-87 0.7506142570118968 1103 88-89 0.8074739515879372 1103 90-91 0.9141095013127334 1103 92-93 0.7960079620276623 1103 94-95 0.7263322287411853 1103 96-97 0.7708284869086057 1103 98-99 0.773642864843243 1103 100-101 0.4799820903802008 1104 1 0.2689813906175438 1104 2 0.2599049681375263 1104 3 0.19154035860259455 1104 4 0.11012445883384459 1104 5 0.14486995315803597 1104 6 0.11593544750012086 1104 7 0.07443486201448479 1104 8 0.18660521580752487 1104 9 0.2755567835458237 1104 10-11 0.30032390807408404 1104 12-13 0.2937779184338041 1104 14-15 0.3823654599640207 1104 16-17 0.366942001098316 1104 18-19 0.38492394751246906 1104 20-21 0.3993185874263645 1104 22-23 0.3689188084396662 1104 24-25 0.32282894398897355 1104 26-27 0.42407176403902014 1104 28-29 0.5117640236202305 1104 30-31 0.4094970985168871 1104 32-33 0.38656932485368856 1104 34-35 0.4416133734526113 1104 36-37 0.4832931123639028 1104 38-39 0.43020312786728 1104 40-41 0.45261252644495187 1104 42-43 0.4884186804944406 1104 44-45 0.41562980802810756 1104 46-47 0.3036138677751907 1104 48-49 0.2890107693971089 1104 50-51 0.44565959219020357 1104 52-53 0.4536538633656164 1104 54-55 0.33897999137766277 1104 56-57 0.5016519687570167 1104 58-59 0.39765090441456863 1104 60-61 0.32540978966950007 1104 62-63 0.5148469109039269 1104 64-65 0.43318822014524727 1104 66-67 0.3591986864534853 1104 68-69 0.3207617290344018 1104 70-71 0.15249794403338868 1104 72-73 0.06839584480248462 1104 74-75 -0.08517636778435289 1104 76-77 0.008023631849550128 1104 78-79 0.07535186896054213 1104 80-81 0.18027040673778671 1104 82-83 0.11529349641117292 1104 84-85 0.13969089119829903 1104 86-87 0.21042691116792156 1104 88-89 0.18421234400074127 1104 90-91 0.15655506191746227 1104 92-93 0.07334552556697815 1104 94-95 0.12172157132952321 1104 96-97 0.10682028634622043 1104 98-99 0.0772708784954581 1104 100-101 0.16912107800933995 1105 1 0.31832527722375303 1105 2 0.3014167555773497 1105 3 0.1864744220138661 1105 4 0.13668605334382988 1105 5 0.15533906479762294 1105 6 0.19205245939403426 1105 7 0.16912622294083235 1105 8 0.23458056238206382 1105 9 0.216601778824959 1105 10-11 0.30013463432263165 1105 12-13 0.32135621390406754 1105 14-15 0.3669179560390603 1105 16-17 0.25393043670356974 1105 18-19 0.32177738089914243 1105 20-21 0.40420510270727306 1105 22-23 0.34208069263522844 1105 24-25 0.38506487179120796 1105 26-27 0.3520073708415268 1105 28-29 0.35429995476992815 1105 30-31 0.4275347267796832 1105 32-33 0.46421471190396346 1105 34-35 0.5125024176065125 1105 36-37 0.4692051863080309 1105 38-39 0.527796330203465 1105 40-41 0.43469069407086636 1105 42-43 0.3809719068373596 1105 44-45 0.39786828559527976 1105 46-47 0.2925358632487658 1105 48-49 0.341084139001687 1105 50-51 0.312903968166097 1105 52-53 0.25936866052516194 1105 54-55 0.2987120452702854 1105 56-57 0.2761461526102593 1105 58-59 0.24189748092782537 1105 60-61 0.18237290330758782 1105 62-63 0.22941562433859986 1105 64-65 0.18638023382565194 1105 66-67 0.3389018216434927 1105 68-69 0.20748895617928298 1105 70-71 0.31779019481345117 1105 72-73 0.3650857442434621 1105 74-75 0.28278794880148794 1105 76-77 0.18731121945617346 1105 78-79 0.2805062757449086 1105 80-81 0.28769070937161345 1105 82-83 0.23084414236349104 1105 84-85 0.3051584086412795 1105 86-87 0.37002344054375413 1105 88-89 0.1917928497421073 1105 90-91 0.31922537565013087 1105 92-93 0.48932163089838454 1105 94-95 0.4595474409026359 1105 96-97 0.6006224464292025 1105 98-99 0.37800069528986313 1105 100-101 0.2807502139880178 1106 1 0.12683186023385318 1106 2 0.12840600125783652 1106 3 0.18774039629992245 1106 4 0.07950391094222908 1106 5 0.06011745748789821 1106 6 -0.046995626838310045 1106 7 0.11711215506146289 1106 8 0.07429810345880128 1106 9 0.036796456788188436 1106 10-11 0.03507462556813579 1106 12-13 0.028585359745093797 1106 14-15 0.1602899372691553 1106 16-17 0.057747408176162196 1106 18-19 0.32565200393043625 1106 20-21 0.22784639987857958 1106 22-23 0.24465079462678574 1106 24-25 0.10229189924650228 1106 26-27 0.07606936705764866 1106 28-29 0.15976713187203018 1106 30-31 0.27663160331263015 1106 32-33 0.14022098727051002 1106 34-35 0.1127884277758966 1106 36-37 -0.21717348104191814 1106 38-39 0.009885687871829418 1106 40-41 0.16340072077826306 1106 42-43 0.15596714468375694 1106 44-45 0.1325486200642203 1106 46-47 0.031278553553697463 1106 48-49 0.07895767305908663 1106 50-51 0.1878222810769543 1106 52-53 0.17421882547268552 1106 54-55 -0.08121919990785642 1106 56-57 0.22633447541628726 1106 58-59 0.2652559146035216 1106 60-61 -0.02552873167039138 1106 62-63 0.07294614735511118 1106 64-65 -0.38744786900539196 1106 66-67 0.06580745570668611 1106 68-69 -0.5818068463264794 1106 70-71 -0.4444544399672985 1106 72-73 -0.12197799893851169 1106 74-75 0.15005107871921908 1106 76-77 0.2538971964169008 1106 78-79 0.19425797059397354 1106 80-81 -0.5898944657492073 1106 82-83 0.02876424719267945 1106 84-85 0.04985921334690602 1106 86-87 0.23291657107396446 1106 88-89 0.30111621935770927 1106 90-91 0.37591922171148084 1106 92-93 0.1838527680781894 1106 94-95 0.325889438265051 1106 96-97 0.19795305564209897 1106 98-99 0.18470507708927997 1106 100-101 -0.0012082757659079846 1107 1 0.1093019623115552 1107 2 0.1735726484348561 1107 3 0.17703176868624126 1107 4 0.14916322654051584 1107 5 0.08481837147233051 1107 6 0.1086023609755884 1107 7 0.06290444666505124 1107 8 0.12833814747870775 1107 9 0.21117093250328622 1107 10-11 0.16004508406612672 1107 12-13 0.13553568890657886 1107 14-15 0.29324858877767923 1107 16-17 0.25786819060279953 1107 18-19 0.2440165393906497 1107 20-21 0.2278269102297159 1107 22-23 0.22148726686151576 1107 24-25 0.24973952949208922 1107 26-27 0.2535621331941442 1107 28-29 0.2785783807633919 1107 30-31 0.23232047162614577 1107 32-33 0.27415113102936317 1107 34-35 0.2904099323874618 1107 36-37 0.2512349728736112 1107 38-39 0.2172871089609103 1107 40-41 0.33006135366322553 1107 42-43 0.40856853223996126 1107 44-45 0.4016333914933625 1107 46-47 0.38106958714050165 1107 48-49 0.3666013424740626 1107 50-51 0.3421087490132848 1107 52-53 0.513841708776674 1107 54-55 0.5402817273188631 1107 56-57 0.5444514122415214 1107 58-59 0.45938937010758707 1107 60-61 0.29940547074109247 1107 62-63 0.3700295347521987 1107 64-65 0.5492432905432807 1107 66-67 0.5327875052477715 1107 68-69 0.5763538434255437 1107 70-71 0.5743381202196751 1107 72-73 0.5869455904247296 1107 74-75 0.6021814041322813 1107 76-77 0.5321013080386621 1107 78-79 0.7221395418761638 1107 80-81 0.8095019424953911 1107 82-83 0.7202827791789304 1107 84-85 0.726052313584038 1107 86-87 0.8662630932929645 1107 88-89 0.7955161757863323 1107 90-91 0.6957042054976945 1107 92-93 0.48703993972432613 1107 94-95 0.269303138366876 1107 96-97 0.5965557813095401 1107 98-99 0.5985906242598347 1107 100-101 0.2746304866360525 1108 1 0.1512113333570433 1108 2 0.12365031186467235 1108 3 0.11188182080028497 1108 4 0.1535970109506195 1108 5 0.07039519124014504 1108 6 0.06215836824533483 1108 7 0.059564988181918466 1108 8 0.09766695762449729 1108 9 0.20772127258019424 1108 10-11 0.14002765291886732 1108 12-13 0.1513258401309514 1108 14-15 0.21018380984787655 1108 16-17 0.15498918291255137 1108 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-0.68792033558573 2316 66-67 -0.6025413097454582 2316 68-69 -0.6036119604644128 2316 70-71 -0.6495533384400183 2316 72-73 -0.5947309252829918 2316 74-75 -0.3503838520299851 2316 76-77 -0.37251577652050116 2316 78-79 -0.47877632295527306 2316 80-81 -0.6516651958535924 2316 82-83 -0.4586839353933776 2316 84-85 -0.5154975090953222 2316 86-87 -0.30356384080710086 2316 88-89 -0.21649938515749767 2316 90-91 -0.2641783541318965 2316 92-93 -0.36375444174537463 2316 94-95 -0.40350466761370285 2316 96-97 -0.2279961094995393 2316 98-99 -0.06428602297899388 2316 100-101 0.07735595055221012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 16.0 4 63.0 5 112.0 6 273.0 7 631.0 8 1485.0 9 2757.0 10 4499.0 11 7127.0 12 10016.0 13 13815.0 14 17635.0 15 23571.0 16 29815.0 17 37483.0 18 34513.0 19 27283.0 20 25547.0 21 26199.0 22 27917.0 23 31218.0 24 36437.0 25 42551.0 26 50962.0 27 62184.0 28 74464.0 29 89300.0 30 107349.0 31 128187.0 32 156455.0 33 194080.0 34 248843.0 35 333232.0 36 446866.0 37 517031.0 38 274702.0 39 14069.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65874456581898 19.28990248406334 15.17698059782365 22.87437235229403 2 15.85199415596471 20.873828569583573 38.91907948372307 24.355097790728646 3 19.0399060760144 24.586436271491436 30.353092740254223 26.020564912239934 4 12.999831541933474 17.236326013475352 36.56327472154398 33.20056772304719 5 13.313034094517107 38.050704600209755 33.954562822935706 14.681698482337433 6 32.01342242893296 36.53742512168029 17.00267919323431 14.446473256152432 7 28.727037906937568 33.42466213185297 20.038732446637198 17.80956751457227 8 27.24977038713729 34.05324953028598 19.641081048983864 19.055899033592862 9 27.10893686178758 16.20360061367789 19.9540838817902 36.73337864274433 10-11 24.96979372600199 26.640287811574815 27.070985389328538 21.318933073094666 12-13 26.386600290315855 24.108785830417588 27.313829632742053 22.190784246524505 14-15 22.66446767573694 25.625505454878734 25.988755262700998 25.72127160668333 16-17 21.756262477030646 28.75206457946477 27.225001887892127 22.26667105561246 18-19 21.94714934743476 26.563739433451637 29.950553362158384 21.538557856955222 20-21 22.551791173959092 25.64457800608902 30.220021725281683 21.583609094670205 22-23 22.31102845263198 25.920887961186228 30.14687800731534 21.62120557886645 24-25 22.025287778821408 26.220382448650486 30.113075184501326 21.641254588026783 26-27 21.32863956210116 26.51137308945733 29.945028655618604 22.214958692822904 28-29 21.592532208730518 26.939720411244224 29.77485030790391 21.69289707212135 30-31 21.602810732521508 26.420356446941838 29.51979124108415 22.457041579452504 32-33 21.119106952800117 26.534969248334946 29.559275617405266 22.78664818145967 34-35 22.352954276360965 26.59443393441927 29.320533462089433 21.73207832713033 36-37 22.528059447075844 25.900808374122086 29.27052376020956 22.300608418592507 38-39 21.719573620331815 26.337648573382477 29.29344761893697 22.649330187348742 40-41 22.339152787706368 26.200631592875943 29.446977019636826 22.013238599780863 42-43 22.021850552137263 26.31181410443007 29.023528037368095 22.642807306064572 44-45 21.88140290115865 26.592227755379444 29.383598181555904 22.142771161906 46-47 22.629404516870654 26.93287881927031 28.224116353534747 22.213600310324285 48-49 22.08165762737463 27.065047403841746 28.06688882477586 22.786406144007763 50-51 22.425366944912923 27.24886678065017 28.059062947164193 22.266703327272715 52-53 22.92864348657854 26.671478371210583 28.164074929631646 22.235803212579235 54-55 22.02510154277899 27.158167279505495 28.403449969567824 22.41328120814769 56-57 22.292214074703733 27.270876052943592 27.871387106955382 22.565522765397294 58-59 22.07266996999381 26.745945227247347 27.870854624561186 23.310530178197652 60-61 21.472997979148634 27.16029720908228 28.44470928720293 22.921995524566157 62-63 21.8212091932924 27.13843315926 28.49427855735352 22.546079090094086 64-65 21.4247679828986 27.27105354707499 28.16049277534342 23.143685694682986 66-67 21.43473416610393 27.231775982934735 28.201781880583503 23.13170797037783 68-69 22.30958605218024 26.595788797331494 28.2600708337643 22.834554316723967 70-71 21.88521601063675 26.86499778938842 27.793583740225706 23.45620245974912 72-73 21.77377336609768 27.00125068839496 27.75652061102526 23.468455334482098 74-75 21.81260879583463 27.46578074504937 27.473687301811665 23.247923157304335 76-77 21.785823317823574 26.841743650712203 28.11715193562191 23.25528109584231 78-79 21.89737031149252 27.07953737929592 27.89100827638999 23.13208403282157 80-81 22.059336322476323 27.18348924219949 27.59753938680238 23.159635048521803 82-83 21.963455288882006 27.14553340626785 27.672243252750373 23.218768052099772 84-85 21.762564445986396 27.263923963821497 27.679912216296465 23.293599373895642 86-87 22.02239260675425 27.375434549404705 27.116076488440633 23.486096355400417 88-89 21.716202099531706 27.380642342201917 26.99147801442609 23.911677543840288 90-91 21.544573761619425 27.247503132883644 27.295121673358437 23.912801432138497 92-93 21.715532610903825 26.82573411868242 27.532006127155746 23.926727143258006 94-95 21.32571807348943 26.593120545598214 27.772021762095957 24.3091396188164 96-97 21.627405227396643 26.03340730536918 27.855110549010416 24.48407691822376 98-99 21.266608007536078 26.410872840440657 27.415392971263536 24.907126180759732 100-101 21.248600418208515 26.101274617834903 27.786291222413574 24.863833741543008 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 732.0 1 509.0 2 390.0 3 553.0 4 529.5 5 501.0 6 861.0 7 1915.5 8 3081.5 9 3505.0 10 3014.5 11 2504.5 12 2598.0 13 2816.5 14 3129.5 15 3460.0 16 3763.0 17 4174.0 18 4672.0 19 5268.0 20 6012.5 21 6970.5 22 8255.5 23 9910.5 24 12012.0 25 15015.0 26 18904.0 27 23319.5 28 27696.0 29 32367.0 30 37658.0 31 45909.5 32 60100.5 33 65268.5 34 62193.5 35 64717.0 36 70458.5 37 76081.0 38 79629.0 39 82467.0 40 86410.0 41 92444.5 42 96825.5 43 99837.0 44 101636.0 45 106709.0 46 109863.0 47 111521.0 48 113547.0 49 110743.0 50 109346.0 51 106737.5 52 102616.0 53 103836.5 54 107626.0 55 102696.0 56 93879.5 57 86284.0 58 81339.5 59 72908.0 60 57432.0 61 46216.5 62 39565.0 63 28907.5 64 18640.5 65 14392.5 66 11235.5 67 8595.0 68 6572.5 69 4696.5 70 3345.5 71 2296.5 72 1700.5 73 1592.0 74 1269.0 75 961.0 76 678.5 77 436.5 78 299.5 79 177.0 80 116.0 81 79.0 82 51.5 83 26.5 84 12.0 85 8.5 86 6.5 87 8.0 88 7.0 89 3.5 90 1.0 91 1.5 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.777048653400433E-4 2 0.04902065192626313 3 5.808898845771799E-4 4 0.0 5 0.08658486446225411 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.1295081089000722E-4 26-27 0.008439039156496252 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010633512053787821 36-37 1.6135830127143888E-4 38-39 0.004550304095854576 40-41 0.005776627185517511 42-43 2.581732820343022E-4 44-45 3.227166025428778E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.8407490381431655E-5 68-69 9.842856377557771E-4 70-71 1.290866410171511E-4 72-73 1.613583012714389E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001145643939027216 82-83 7.745198461029065E-4 84-85 0.005453910582974634 86-87 0.0026462761408515972 88-89 9.681498076286331E-5 90-91 0.0035821542882259433 92-93 9.520139775014893E-4 94-95 1.1295081089000722E-4 96-97 1.613583012714389E-5 98-99 1.613583012714389E-5 100-101 1.613583012714389E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3098694.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.90993481998355 #Duplication Level Percentage of deduplicated Percentage of total 1 70.92664138454488 31.14384200195713 2 14.635985235510388 12.853303154350055 3 5.662717450013849 7.4594866240227455 4 2.7985347775383747 4.915339186926688 5 1.5686714025584643 3.4440129520157168 6 1.0042147662319152 2.6457002958305047 7 0.6718891852930644 2.0651827232729216 8 0.4419195406388339 1.5523726580102613 9 0.3257102661722041 1.2871724902038877 >10 1.7468655662177839 13.636869917623917 >50 0.12661134556160517 3.853593796920755 >100 0.07736363439297715 6.489530736571984 >500 0.007504545275598401 2.229133576402318 >1k 0.004855882237005101 4.667062664350905 >5k 3.678698664397804E-4 0.976974325831775 >10k+ 1.4714794657591214E-4 0.7804228957084154 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13354 0.4309557510357589 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10803 0.34863074572707087 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7452 0.24048841221495248 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6179 0.19940658871124414 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5998 0.19356541820521805 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5357 0.1728792839822196 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5255 0.16958757463628227 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4871 0.15719525709863574 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4617 0.14899825539404665 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4591 0.14815919222743518 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3829 0.12356818711366788 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3820 0.1232777421713793 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3796 0.1225032223252764 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3795 0.12247095066502209 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3618 0.11675886680001318 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3597 0.11608116193467313 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3533 0.1140157756783987 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3480 0.11230537768492145 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3438 0.11094996795424138 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.681498076286332E-5 2 0.0 0.0 0.0 0.0 9.681498076286332E-5 3 0.0 0.0 0.0 0.0 9.681498076286332E-5 4 0.0 0.0 0.0 0.0 9.681498076286332E-5 5 0.0 0.0 0.0 0.0 9.681498076286332E-5 6 0.0 0.0 0.0 0.0 1.290866410171511E-4 7 0.0 0.0 0.0 0.0 1.290866410171511E-4 8 0.0 0.0 0.0 0.0 1.6135830127143888E-4 9 0.0 0.0 0.0 0.0 1.6135830127143888E-4 10-11 0.0 0.0 0.0 0.0 1.6135830127143888E-4 12-13 0.0 0.0 0.0 0.0 2.420374519071583E-4 14-15 0.0 0.0 0.0 0.0 2.7430911216144607E-4 16-17 0.0 0.0 0.0 3.227166025428778E-5 3.2271660254287775E-4 18-19 0.0 0.0 0.0 6.454332050857555E-5 3.2271660254287775E-4 20-21 0.0 1.613583012714389E-5 0.0 1.7749413139858276E-4 3.2271660254287775E-4 22-23 0.0 3.227166025428778E-5 0.0 5.486182243228921E-4 3.2271660254287775E-4 24-25 0.0 3.227166025428778E-5 0.0 0.0011133722787729282 3.2271660254287775E-4 26-27 0.0 3.227166025428778E-5 0.0 0.0027430911216144607 3.2271660254287775E-4 28-29 0.0 3.227166025428778E-5 0.0 0.007019086105307591 3.2271660254287775E-4 30-31 0.0 3.227166025428778E-5 0.0 0.02246107553698429 3.2271660254287775E-4 32-33 0.0 3.227166025428778E-5 0.0 0.05339346189071913 3.549882627971655E-4 34-35 0.0 3.227166025428778E-5 0.0 0.0980574396826534 4.1953158330574106E-4 36-37 0.0 3.227166025428778E-5 0.0 0.1747510402769683 4.1953158330574106E-4 38-39 0.0 3.227166025428778E-5 0.0 0.2896058791219785 4.5180324356002883E-4 40-41 0.0 3.227166025428778E-5 0.0 0.40944668947627616 4.840749038143166E-4 42-43 0.0 3.227166025428778E-5 0.0 0.5333698648527412 4.840749038143166E-4 44-45 0.0 3.227166025428778E-5 0.0 0.6879511174707796 5.486182243228921E-4 46-47 0.0 3.227166025428778E-5 0.0 0.8686401432345369 6.292973749586116E-4 48-49 0.0 3.227166025428778E-5 0.0 1.0606887934078033 6.777048653400432E-4 50-51 0.0 3.227166025428778E-5 0.0 1.268437606294781 7.422481858486188E-4 52-53 0.0 3.227166025428778E-5 0.0 1.464907473922885 7.745198461029066E-4 54-55 0.0 3.227166025428778E-5 0.0 1.6826282298284374 7.745198461029066E-4 56-57 0.0 3.227166025428778E-5 0.0 1.9330563134017105 7.745198461029066E-4 58-59 0.0 3.227166025428778E-5 0.0 2.198974793897042 7.745198461029066E-4 60-61 0.0 3.227166025428778E-5 0.0 2.4948413751083525 7.906556762300504E-4 62-63 0.0 3.227166025428778E-5 0.0 2.7828336712176163 8.067915063571943E-4 64-65 0.0 3.227166025428778E-5 0.0 3.0820565050953723 8.874706569929139E-4 66-67 0.0 3.227166025428778E-5 0.0 3.4291866186206192 9.197423172472016E-4 68-69 0.0 3.227166025428778E-5 0.0 3.812961202364609 9.358781473743455E-4 70-71 0.0 3.227166025428778E-5 0.0 4.209434684418662 9.681498076286332E-4 72-73 0.0 3.227166025428778E-5 0.0 4.623012146407486 0.0010326931281372086 74-75 0.0 4.840749038143167E-5 0.0 5.039607008630087 0.0010811006185186404 76-77 0.0 6.454332050857555E-5 0.0 5.493991985010459 0.0010972364486457843 78-79 0.0 6.454332050857555E-5 0.0 5.995913116945397 0.0010972364486457843 80-81 0.0 6.454332050857555E-5 0.0 6.516471132677186 0.0010972364486457843 82-83 0.0 6.454332050857555E-5 0.0 7.062668982481007 0.001129508108900072 84-85 0.0 6.454332050857555E-5 0.0 7.621710953066033 0.001129508108900072 86-87 0.0 8.067915063571944E-5 0.0 8.20042250057605 0.00116177976915436 88-89 0.0 9.681498076286332E-5 0.0 8.802756903392204 0.0011940514294086475 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7195 0.0 54.27176 1 GTATCAA 13315 0.0 42.150463 1 ATCAACG 17665 0.0 31.565115 3 TATCAAC 17680 0.0 31.55566 2 TCAACGC 17845 0.0 31.246218 4 CAACGCA 18375 0.0 30.344965 5 AACGCAG 19055 0.0 29.31229 6 ACGCAGA 21445 0.0 26.001205 7 TAAGGTG 2205 0.0 25.869535 5 CGCAGAG 21595 0.0 25.776611 8 TACATGG 15275 0.0 24.598433 2 GTACATG 15595 0.0 24.429167 1 TAAGGTA 1235 0.0 24.248762 4 ACATGGG 15425 0.0 23.575409 3 ATGGGCG 510 0.0 23.301603 5 GTGTAAG 1290 0.0 23.22803 1 GCAGAGT 23575 0.0 23.128187 9 CGTATAG 125 2.237206E-4 22.829832 1 TGGTATC 3235 0.0 21.906527 2 AGGTAAG 2270 0.0 21.79059 2 >>END_MODULE