FastQCFastQC Report
Thu 26 May 2016
SRR938661_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938661_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096214
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140300.45313405339553403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106740.34474361268310266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72970.2356749242784898No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA59980.1937204598906923No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA59270.19142733674093584No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA54480.17595682985736774No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA47680.1539945236343483No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47370.1529933008506518No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC46450.15002193000871386No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG41320.13345330781399478No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT40830.1318707298655713No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA39130.12638015330981642No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG38610.12470068283393848No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA37850.12224607213842453No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA36750.11869334613175964No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG35890.1159157603447307No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT34860.11258911690212627No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT33920.10955315104188536No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76550.051.3619651
GTATCAA139000.042.2404751
ATCAACG180300.032.3651283
TATCAAC180200.032.3567162
TCAACGC182850.031.887784
CAACGCA186350.031.2378675
AACGCAG193350.030.129086
TAAGGTG22450.028.9987245
AGGTAAG22150.026.8170452
CGCAGAG217250.026.6343988
ACGCAGA217300.026.628277
GTAAGGT23250.026.5702134
GGTAAGG24550.025.5503643
GTACATG159900.025.4233881
TACATGG159450.025.153162
GCAGAGT231000.024.246179
TGGTATC35950.024.1894972
ACATGGG163750.023.854223
GAGTACT128950.022.76603512-13
GTGGTAT39850.022.6030161
Produced by FastQC (version 0.11.5)