FastQCFastQC Report
Thu 26 May 2016
SRR938661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938661_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096214
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137120.44286344548535733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106720.34467901766479964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74490.24058414566951766No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA60650.19588439300384275No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA60510.19543222787572176No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC54080.17466492949130777No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA52630.16998179066434038No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48070.15525412649125672No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46760.15102315279241035No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA46180.1491498972616234No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38800.12531433550781698No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG38540.12447460026987799No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG38520.12441000525157499No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT37640.12156782444624305No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA35540.11478534752442822No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT35040.11317047206685325No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA34830.11249222437467177No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33950.10965004356933986No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA32870.10616191258097793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72850.053.2766151
GTATCAA130950.041.8430181
TATCAAC172900.031.6082942
ATCAACG174000.031.342143
TCAACGC175650.031.1018644
TAAGGTG22850.030.379545
CAACGCA181050.030.1216935
AACGCAG188250.029.0396736
AGGTAAG23550.026.4579622
GTAAGGT25650.026.3217964
GGTAAGG24650.025.6536523
ACGCAGA213350.025.4674117
CGCAGAG215200.025.2043348
GTACATG155650.024.2020451
TACATGG153700.024.0449122
AAGGTAA25050.023.354651
GGACCGA10550.022.9598546
GCAGAGT234700.022.8673959
TAAGGTA12350.022.714314
ACATGGG159050.022.6295913
Produced by FastQC (version 0.11.5)