##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938661_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3096214 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.822046538126887 31.0 31.0 34.0 30.0 34.0 2 32.05154811650616 33.0 31.0 34.0 30.0 34.0 3 32.110699066666584 33.0 31.0 34.0 30.0 34.0 4 35.784740008281084 37.0 35.0 37.0 35.0 37.0 5 35.65935526420331 37.0 35.0 37.0 33.0 37.0 6 35.70401690580819 37.0 35.0 37.0 33.0 37.0 7 35.63892612073972 37.0 35.0 37.0 33.0 37.0 8 35.619497554109635 37.0 35.0 37.0 33.0 37.0 9 37.18967358199401 39.0 37.0 39.0 33.0 39.0 10-11 37.12795433390586 39.0 37.0 39.0 33.0 39.0 12-13 37.03144210962162 39.0 37.0 39.0 33.0 39.0 14-15 38.07509429257797 40.0 37.0 41.0 33.0 41.0 16-17 38.01251496182111 40.0 37.0 41.0 33.0 41.0 18-19 38.04884998259164 40.0 37.0 41.0 33.0 41.0 20-21 38.049335252666644 40.0 37.0 41.0 33.0 41.0 22-23 38.06665414599895 40.0 37.0 41.0 33.0 41.0 24-25 37.98691644053027 40.0 37.0 41.0 33.0 41.0 26-27 37.81161718795923 40.0 37.0 41.0 32.5 41.0 28-29 37.681267670774695 40.0 37.0 41.0 32.0 41.0 30-31 37.42081861912646 39.0 36.0 41.0 32.0 41.0 32-33 37.17243010334557 39.0 36.0 41.0 31.0 41.0 34-35 36.98859914075707 39.0 35.5 41.0 31.0 41.0 36-37 36.96395501086165 39.0 36.0 41.0 31.0 41.0 38-39 36.797106078585 39.0 35.0 41.0 30.0 41.0 40-41 36.606884246373156 39.0 35.0 41.0 30.0 41.0 42-43 36.34730351325845 39.0 35.0 40.5 30.0 41.0 44-45 36.031352161058635 39.0 35.0 40.0 29.5 41.0 46-47 35.61509136642364 38.0 35.0 40.0 28.0 41.0 48-49 35.17296268927148 38.0 34.0 40.0 26.5 41.0 50-51 34.790185045348935 38.0 34.0 40.0 25.5 41.0 52-53 34.29047426954338 38.0 33.0 40.0 24.0 41.0 54-55 33.97548183039028 37.5 33.0 40.0 23.0 41.0 56-57 33.61864360796767 37.0 33.0 40.0 23.0 41.0 58-59 33.17388995075922 36.5 32.0 39.5 21.5 41.0 60-61 32.778902394989494 36.0 31.5 39.0 20.0 40.5 62-63 32.32553305423979 35.5 31.0 39.0 18.5 40.0 64-65 31.851266094656246 35.0 31.0 38.5 17.0 40.0 66-67 31.382730005096548 35.0 30.0 38.0 13.0 40.0 68-69 30.846125138637056 34.5 29.5 37.5 9.0 40.0 70-71 30.30262992157519 34.0 29.0 37.0 7.5 39.0 72-73 29.765309342312904 34.0 28.5 36.0 6.5 39.0 74-75 29.20215479291806 34.0 28.0 36.0 2.0 38.5 76-77 27.919177259711375 31.5 26.5 34.5 2.0 36.5 78-79 28.57548476946361 33.0 27.5 35.0 2.0 37.0 80-81 28.335916541944453 33.0 27.0 35.0 2.0 37.0 82-83 27.885321557230863 33.0 26.0 35.0 2.0 36.0 84-85 27.348901916986357 33.0 26.0 35.0 2.0 36.0 86-87 26.774729072344485 32.5 25.0 35.0 2.0 35.0 88-89 26.064185970349598 32.0 23.5 34.0 2.0 35.0 90-91 25.572498864742553 32.0 22.0 34.0 2.0 35.0 92-93 24.870633618994034 31.0 19.0 34.0 2.0 35.0 94-95 24.15288413526972 31.0 12.0 34.0 2.0 35.0 96-97 23.245108542239006 31.0 2.0 34.0 2.0 35.0 98-99 22.125286559649947 30.0 2.0 34.0 2.0 35.0 100-101 18.948880794415373 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30250231813224104 1101 2 0.37579346612020004 1101 3 0.32580494304603747 1101 4 0.2018525271112992 1101 5 0.09198739630286212 1101 6 0.22015246535043786 1101 7 0.2322251183374604 1101 8 0.26980711170759264 1101 9 0.31017926753299463 1101 10-11 0.3242712908922556 1101 12-13 0.31630761265508056 1101 14-15 0.53191720220061 1101 16-17 0.5127232029288322 1101 18-19 0.5772935070173233 1101 20-21 0.4733996174091004 1101 22-23 0.4593644323562316 1101 24-25 0.47157447079347037 1101 26-27 0.4587230742789714 1101 28-29 0.344698333129287 1101 30-31 0.4494929828771319 1101 32-33 0.4080130957219268 1101 34-35 0.19223489260740223 1101 36-37 0.3567566527263253 1101 38-39 0.42361391908927004 1101 40-41 0.5533099776859842 1101 42-43 0.334056658486638 1101 44-45 0.3819075482899379 1101 46-47 0.4480137593794211 1101 48-49 0.4947855009706146 1101 50-51 0.5793659779557601 1101 52-53 0.5408503914383189 1101 54-55 0.5498845203951461 1101 56-57 0.5119023052736082 1101 58-59 0.6937791399918964 1101 60-61 0.7890542905955513 1101 62-63 0.745614932349028 1101 64-65 0.7528615730737869 1101 66-67 0.7997262744807934 1101 68-69 0.8723956992901947 1101 70-71 0.8714554781207795 1101 72-73 1.0460290675707213 1101 74-75 1.0524835851145546 1101 76-77 0.8749574743132449 1101 78-79 1.0470688681615208 1101 80-81 1.3712313843287873 1101 82-83 1.4788121239198944 1101 84-85 1.7904674122749746 1101 86-87 1.86399771644205 1101 88-89 2.133062916269516 1101 90-91 2.3637642248061823 1101 92-93 2.5436260354830935 1101 94-95 2.40912620418678 1101 96-97 3.0226608890532702 1101 98-99 3.130966005409725 1101 100-101 3.7243004577118732 1102 1 0.32001404639652975 1102 2 0.2938771577406243 1102 3 0.30369528209114804 1102 4 0.21853798063479957 1102 5 0.13062974638864233 1102 6 0.1461603123582762 1102 7 0.25467321824230993 1102 8 0.3311231876648648 1102 9 0.37574519378728155 1102 10-11 0.29784289790019614 1102 12-13 0.3395914776738138 1102 14-15 0.5160129441457357 1102 16-17 0.47753749163454984 1102 18-19 0.47344127273859016 1102 20-21 0.39937440118166734 1102 22-23 0.4596341426365811 1102 24-25 0.3971670374434382 1102 26-27 0.4779649587854138 1102 28-29 0.4320524722177481 1102 30-31 0.4284156296013606 1102 32-33 0.3492862078418071 1102 34-35 0.5374456064426596 1102 36-37 0.6245601334278135 1102 38-39 0.766544800395927 1102 40-41 0.7158700425633953 1102 42-43 0.7224077765750465 1102 44-45 0.5809561077737655 1102 46-47 0.6325236795745681 1102 48-49 0.9097281852399774 1102 50-51 0.9759638475812977 1102 52-53 1.0125412207989726 1102 54-55 0.8989395241484388 1102 56-57 0.973277252158141 1102 58-59 1.0011029068250608 1102 60-61 1.09527564344517 1102 62-63 1.3622401373104793 1102 64-65 1.260018385875867 1102 66-67 1.1907487029876656 1102 68-69 1.3249928929562635 1102 70-71 1.572147862065762 1102 72-73 1.5046664244956958 1102 74-75 1.5199778327238995 1102 76-77 1.422358826477108 1102 78-79 1.7961754719917131 1102 80-81 1.9545207353954446 1102 82-83 1.8708550736747966 1102 84-85 2.0446137103559607 1102 86-87 2.077258262695679 1102 88-89 2.2906861502947713 1102 90-91 2.620856365307791 1102 92-93 2.875199159726158 1102 94-95 2.779195496155964 1102 96-97 3.1801532477722283 1102 98-99 3.396996470119621 1102 100-101 4.130480236604544 1103 1 0.27349000123049194 1103 2 0.24381785207496165 1103 3 0.21419703619939412 1103 4 0.1365265352147773 1103 5 0.10678160514020618 1103 6 0.18450857204813786 1103 7 0.1636731096415076 1103 8 0.21784887067823888 1103 9 0.23661156765184188 1103 10-11 0.30174700466709936 1103 12-13 0.3488696249979455 1103 14-15 0.40910395090914875 1103 16-17 0.4883127583472131 1103 18-19 0.3821804938966906 1103 20-21 0.4071806324322793 1103 22-23 0.33852725835108544 1103 24-25 0.36732029483739836 1103 26-27 0.3172961619194652 1103 28-29 0.39654666504831226 1103 30-31 0.4400672070810927 1103 32-33 0.5308591722174825 1103 34-35 0.5363301473663995 1103 36-37 0.6248093347446684 1103 38-39 0.5501006426588546 1103 40-41 0.6391357890069997 1103 42-43 0.5034947559053577 1103 44-45 0.6083873675019973 1103 46-47 0.5020392506215074 1103 48-49 0.6873967171879443 1103 50-51 0.5884410035372838 1103 52-53 0.7280219181038987 1103 54-55 0.6760598839951442 1103 56-57 0.6216200704806027 1103 58-59 0.7544471247810662 1103 60-61 0.6764946003592556 1103 62-63 0.70871010862259 1103 64-65 0.6627616251220161 1103 66-67 0.7255518615577223 1103 68-69 0.8482719773339831 1103 70-71 0.7995822226512885 1103 72-73 0.8413735094448853 1103 74-75 0.8865208285173516 1103 76-77 0.7303061679733922 1103 78-79 0.9478238183498284 1103 80-81 1.1117868723488407 1103 82-83 1.1819487233880785 1103 84-85 1.353020375188546 1103 86-87 1.3363042350997496 1103 88-89 1.5388856353825489 1103 90-91 1.7834871269732098 1103 92-93 1.8516214585431747 1103 94-95 1.8787312454299894 1103 96-97 2.2355910541323247 1103 98-99 2.517879710382399 1103 100-101 3.073837910015744 1104 1 0.27714028347035935 1104 2 0.3204728656933824 1104 3 0.33618423868097125 1104 4 0.20836235158994754 1104 5 0.15386507869418864 1104 6 0.15358373688433602 1104 7 0.244909309678647 1104 8 0.2644897276111635 1104 9 0.305160381466699 1104 10-11 0.35597908896990305 1104 12-13 0.37059926949407185 1104 14-15 0.4876736859276676 1104 16-17 0.5493569238393761 1104 18-19 0.3491196942664416 1104 20-21 0.47242202225368857 1104 22-23 0.3077491573825597 1104 24-25 0.4216066623107082 1104 26-27 0.4860079948807581 1104 28-29 0.4744390348310148 1104 30-31 0.48232339311160644 1104 32-33 0.3621503404891868 1104 34-35 0.4418374359911894 1104 36-37 0.48407382127219734 1104 38-39 0.5635697848294825 1104 40-41 0.6008650446477191 1104 42-43 0.6426470537341444 1104 44-45 0.6675121140119771 1104 46-47 0.6468795373984548 1104 48-49 0.8504176904564886 1104 50-51 0.7115898136723686 1104 52-53 0.9542975302021048 1104 54-55 0.9150821892891727 1104 56-57 1.1247677114397163 1104 58-59 1.2471478651852408 1104 60-61 1.193924195674306 1104 62-63 1.2164833020532342 1104 64-65 1.244866029666298 1104 66-67 1.391867862422604 1104 68-69 1.5767307906960504 1104 70-71 1.6596434060478913 1104 72-73 1.8068581557241323 1104 74-75 1.9214494389712762 1104 76-77 1.855555384488472 1104 78-79 2.1034718060818314 1104 80-81 2.2982325343079673 1104 82-83 2.273943234524417 1104 84-85 2.3806463333484835 1104 86-87 2.582898129260876 1104 88-89 2.618984629095227 1104 90-91 2.7266912067710365 1104 92-93 2.9798888062896474 1104 94-95 3.2957303856419813 1104 96-97 3.461630808464445 1104 98-99 3.756555242778294 1104 100-101 4.2712128228658095 1105 1 0.27265832292241043 1105 2 0.28498774039637453 1105 3 0.21194381908524207 1105 4 0.1760345665653631 1105 5 0.04615869245905202 1105 6 0.15507553296188092 1105 7 0.18266111897730042 1105 8 0.21014181839610302 1105 9 0.2703274688412094 1105 10-11 0.33053407152578984 1105 12-13 0.41555504139697064 1105 14-15 0.4685887577523218 1105 16-17 0.4235570730667817 1105 18-19 0.4127383919537948 1105 20-21 0.36299601959321137 1105 22-23 0.2807754791468611 1105 24-25 0.16568554395598056 1105 26-27 0.3629882932304085 1105 28-29 0.5081493960024446 1105 30-31 0.6018873116263705 1105 32-33 0.48789826997087005 1105 34-35 0.5175214706184477 1105 36-37 0.6214260099009934 1105 38-39 0.6268435554599776 1105 40-41 0.5618346332303474 1105 42-43 0.7232039821733451 1105 44-45 0.8817451558850422 1105 46-47 0.9551490276592389 1105 48-49 0.9665189547787563 1105 50-51 0.9380059564259255 1105 52-53 0.9974983744219728 1105 54-55 1.0894969186364492 1105 56-57 1.1537549080915142 1105 58-59 1.3397536997760753 1105 60-61 1.3866315904610929 1105 62-63 1.4070859427378224 1105 64-65 1.4145498272122268 1105 66-67 1.397571616385708 1105 68-69 1.2942346946513403 1105 70-71 1.3102357031031637 1105 72-73 1.4773257317443118 1105 74-75 1.6524529980592568 1105 76-77 1.4554198035985664 1105 78-79 1.7218979373206942 1105 80-81 1.8909200448535444 1105 82-83 2.01794707227857 1105 84-85 2.0891886463924045 1105 86-87 2.211302110602194 1105 88-89 2.3657858234880607 1105 90-91 2.530095928991532 1105 92-93 2.834682402130017 1105 94-95 2.7707840874328546 1105 96-97 3.1081390090409933 1105 98-99 3.536352029179003 1105 100-101 3.9822836251041274 1106 1 0.16875061043593576 1106 2 0.17511745020937042 1106 3 0.15779310050238138 1106 4 0.13016311272578918 1106 5 0.11160204728586365 1106 6 0.07773921955754304 1106 7 0.17120361672922968 1106 8 0.04038680653808768 1106 9 0.18580155907543627 1106 10-11 0.2503876147976314 1106 12-13 0.1029175484014857 1106 14-15 0.03993885961759247 1106 16-17 0.11208363224934459 1106 18-19 0.17140253432860675 1106 20-21 0.3868785843053999 1106 22-23 0.195309296543833 1106 24-25 0.16369407908271683 1106 26-27 0.1564128455689726 1106 28-29 0.2377532791676984 1106 30-31 0.26319922029517073 1106 32-33 0.2899433505474178 1106 34-35 0.24248490096393027 1106 36-37 0.3654109125592626 1106 38-39 0.34574033929416004 1106 40-41 0.3783544694421366 1106 42-43 0.4894959744808105 1106 44-45 0.06924284387909552 1106 46-47 -0.05532150494016008 1106 48-49 0.36340394635505646 1106 50-51 0.2400211807281778 1106 52-53 0.41950744935927986 1106 54-55 0.3684489988024282 1106 56-57 0.41310540225566683 1106 58-59 0.8845951977823887 1106 60-61 0.7175053871333574 1106 62-63 0.8591012583785869 1106 64-65 0.5619048695773046 1106 66-67 0.5992119303709593 1106 68-69 0.4302462366417039 1106 70-71 0.5782756289911006 1106 72-73 0.994083493348068 1106 74-75 1.3140953808616764 1106 76-77 1.3293138655452559 1106 78-79 1.477473639359868 1106 80-81 1.4956390145369234 1106 82-83 1.7968925304028325 1106 84-85 1.7693146889643678 1106 86-87 2.0995331782990974 1106 88-89 2.179416223135128 1106 90-91 2.1163775931973845 1106 92-93 1.7074017937972954 1106 94-95 2.337024085295532 1106 96-97 2.8773913965153746 1106 98-99 3.2524957562558896 1106 100-101 3.833259771139783 1107 1 0.1846826870483369 1107 2 0.18436133513791475 1107 3 0.15222313195904746 1107 4 0.16338379718096974 1107 5 0.16360817201846345 1107 6 0.07711618345236815 1107 7 0.09654094075695951 1107 8 0.13580472163857138 1107 9 0.23703726166350236 1107 10-11 0.20114462942846245 1107 12-13 0.25927824894998963 1107 14-15 0.31978587441158624 1107 16-17 0.30183263801081495 1107 18-19 0.26623750704984417 1107 20-21 0.24852125485026733 1107 22-23 0.25231200123331377 1107 24-25 0.16771509871500712 1107 26-27 0.28617015132211776 1107 28-29 0.33552878049493273 1107 30-31 0.36594269387389033 1107 32-33 0.2726083692385046 1107 34-35 0.2857661586123328 1107 36-37 0.3430193448870682 1107 38-39 0.3935256735137429 1107 40-41 0.4160456311747538 1107 42-43 0.48203516005970215 1107 44-45 0.5719469966229909 1107 46-47 0.4700959850702233 1107 48-49 0.5154740278263716 1107 50-51 0.6214741773558004 1107 52-53 0.6815052418964243 1107 54-55 0.5667816580029665 1107 56-57 0.47966533480803264 1107 58-59 0.5639761377725492 1107 60-61 0.5618752833684297 1107 62-63 0.7502756685553251 1107 64-65 0.9044963111823776 1107 66-67 0.9681153760912444 1107 68-69 0.93188524883535 1107 70-71 0.8568793088973905 1107 72-73 1.0107140702940995 1107 74-75 0.9743322913067836 1107 76-77 0.7980880989016903 1107 78-79 1.070880642096764 1107 80-81 1.3915368091351006 1107 82-83 1.432359730192605 1107 84-85 1.624590830073675 1107 86-87 1.891158838798951 1107 88-89 2.0979028873285444 1107 90-91 2.1582167648642283 1107 92-93 2.4298569210280547 1107 94-95 2.6700522339894768 1107 96-97 2.8437999926960913 1107 98-99 2.980057332060621 1107 100-101 3.379873423917598 1108 1 0.16022684556332223 1108 2 0.17412261519773864 1108 3 0.09408951090060924 1108 4 0.1380616908304262 1108 5 0.02305857904808306 1108 6 0.04647567822229348 1108 7 0.0978080561226804 1108 8 0.09717572541259045 1108 9 0.24225109084731855 1108 10-11 0.20863265144513576 1108 12-13 0.2611878341885472 1108 14-15 0.32924745138597444 1108 16-17 0.3044134101788032 1108 18-19 0.30475024870882805 1108 20-21 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2316 44-45 -0.6295904411552655 2316 46-47 -0.5701436242197175 2316 48-49 -0.42416561136820974 2316 50-51 -0.3958908516036175 2316 52-53 -0.37752509517630983 2316 54-55 -0.07942223104949875 2316 56-57 -0.039538557867231816 2316 58-59 -0.02491919532497633 2316 60-61 0.08828119671479584 2316 62-63 0.07708675191352654 2316 64-65 0.05723990556214886 2316 66-67 0.09105362728891109 2316 68-69 0.1326835100846644 2316 70-71 0.12950963780233593 2316 72-73 0.3423083729988896 2316 74-75 0.6375262897614924 2316 76-77 0.53025918572461 2316 78-79 0.629579615656187 2316 80-81 0.7890951347123298 2316 82-83 0.9490872271148625 2316 84-85 1.0877320425553698 2316 86-87 1.2144117885887944 2316 88-89 1.3843944125903924 2316 90-91 1.4340337247478772 2316 92-93 1.2964210457343555 2316 94-95 1.825038223599261 2316 96-97 2.0244477789292894 2316 98-99 2.3222147576003707 2316 100-101 2.733575547674068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 23.0 4 48.0 5 107.0 6 256.0 7 630.0 8 1507.0 9 2568.0 10 4421.0 11 7095.0 12 9951.0 13 13655.0 14 17478.0 15 23603.0 16 30418.0 17 38660.0 18 36031.0 19 28583.0 20 27585.0 21 28149.0 22 30560.0 23 34436.0 24 40124.0 25 46401.0 26 55023.0 27 65714.0 28 78962.0 29 94998.0 30 113139.0 31 135168.0 32 162895.0 33 200764.0 34 254199.0 35 329483.0 36 423395.0 37 476044.0 38 266847.0 39 17288.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60496851961783 19.27399075128528 15.197915906329474 22.92312482276742 2 15.864980402674153 20.85814638055325 38.92293872644848 24.35393449032412 3 19.022302406197504 24.572211278746003 30.350657399862346 26.05482891519414 4 13.016509840728062 17.21986916924993 36.523670521482046 33.239950468539966 5 13.27813668138563 38.05584886432926 33.91005417932133 14.755960274963773 6 32.019427597704805 36.56129711964354 17.002636122696945 14.416639159954705 7 28.695335658323355 33.45585931721774 20.042187006453688 17.806618018005217 8 27.23800745038941 34.0615345063358 19.647059279494247 19.053398763780542 9 27.083593059136092 16.18883578460662 19.9778503682239 36.749720788033386 10-11 24.953653074367597 26.641989216507646 27.078958366572852 21.325399342551904 12-13 26.35333022846612 24.11703454606174 27.332978276049396 22.196656949422746 14-15 22.66681179014112 25.60880804750576 26.01693552189868 25.70744464045444 16-17 21.772235381662895 28.729603315533097 27.207470155486668 22.290691147317336 18-19 21.934837191486118 26.586954260913494 29.925127914284992 21.553080633315396 20-21 22.56906337869411 25.64646694317641 30.175772734055208 21.60869694407428 22-23 22.326767465039563 25.884467288113804 30.143523671167433 21.6452415756792 24-25 22.016215481220875 26.19785625950971 30.11213506204587 21.673793197223542 26-27 21.314966820505134 26.521227512106382 29.94200694598771 22.221798721400774 28-29 21.6366504382449 26.950527321431917 29.747087895087354 21.665734345235826 30-31 21.625959962715754 26.41256386024997 29.54072296036385 22.420753216670423 32-33 21.16935369917455 26.54362326692661 29.515252176258134 22.771770857640703 34-35 22.38737653266179 26.56183073741552 29.301676681446033 21.749116048476658 36-37 22.53178171388706 25.867521284704726 29.27956603749209 22.32113096391613 38-39 21.726700039326875 26.325117758545925 29.266751673007146 22.681430529120057 40-41 22.337379903368753 26.197171088533207 29.441603050907844 22.023845957190197 42-43 21.995935133319392 26.28468439951971 29.062633991619947 22.656746475540952 44-45 21.915818552397692 26.531963172776624 29.40517584270833 22.14704243211735 46-47 22.626881695078275 26.95438069304708 28.197109305535058 22.22162830633959 48-49 22.082646742117955 27.06020966251041 28.036547215405655 22.820596379965984 50-51 22.424822702823512 27.255108981485133 28.071993731699425 22.248074583991933 52-53 22.930811629945474 26.685913828953684 28.170807960948434 22.212466580152405 54-55 22.03938422860952 27.15195073725524 28.41291008954807 22.39575494458716 56-57 22.298523293286575 27.256497774378648 27.894761150230572 22.55021778210421 58-59 22.079368544939076 26.756919902823256 27.90383675030214 23.259874801935528 60-61 21.472998959374255 27.166710698937475 28.441105815037332 22.919184526650934 62-63 21.812768755648026 27.149350787768544 28.478603223162224 22.559277233421206 64-65 21.44452870505721 27.2458072988495 28.151978513113114 23.15768548298018 66-67 21.415263673381062 27.198548546891644 28.235995165052728 23.150192614674566 68-69 22.314446099335335 26.61756253896902 28.253175374599167 22.81481598709648 70-71 21.886746200359536 26.882718700968343 27.77642307669948 23.45411202197264 72-73 21.78979611544813 27.026144519127076 27.73039193362989 23.453667431794905 74-75 21.822571049675506 27.481643710673747 27.44393636873937 23.251848870911378 76-77 21.834165855460896 26.81293993244653 28.08021344777848 23.272680764314092 78-79 21.89596713922229 27.092636361698514 27.872378976388585 23.139017522690615 80-81 22.06947292232916 27.184284933839347 27.609481710482818 23.13676043334867 82-83 21.937355891387767 27.198614167287595 27.70131280745458 23.16271713387006 84-85 21.773961856759772 27.29731104610085 27.659606423257703 23.269120673881673 86-87 22.016929292041915 27.393697766144776 27.14968677450736 23.43968616730595 88-89 21.752741737322896 27.390227154988096 26.940710132251926 23.916320975437085 90-91 21.53431549790977 27.229608274819185 27.33480212848798 23.90127409878307 92-93 21.682972644585018 26.828415163590535 27.586552703534583 23.90205948828986 94-95 21.33153586929069 26.585759253074887 27.81832909482355 24.26437578281088 96-97 21.65751812657622 26.07423551379774 27.83333578256151 24.434910577064535 98-99 21.270380535712324 26.45500278727504 27.40526010153045 24.869356575482186 100-101 21.233157326721336 26.06913237412233 27.84925539949819 24.848454899658144 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 719.0 1 511.5 2 418.5 3 556.5 4 515.0 5 529.0 6 921.0 7 2007.5 8 3189.0 9 3553.5 10 3117.0 11 2689.0 12 2740.0 13 2921.0 14 3182.0 15 3498.5 16 3804.5 17 4261.0 18 4772.0 19 5291.5 20 6014.5 21 7069.5 22 8426.0 23 10128.0 24 12276.5 25 15138.0 26 19164.5 27 23816.5 28 27938.5 29 32170.5 30 37187.5 31 45546.5 32 59602.5 33 64774.5 34 62009.5 35 64507.0 36 70192.0 37 75820.5 38 79445.5 39 82084.0 40 86021.5 41 91890.0 42 95916.5 43 99136.5 44 101519.0 45 106882.5 46 109972.5 47 111661.0 48 113357.5 49 110137.0 50 108752.5 51 106395.5 52 102538.5 53 104008.0 54 107684.0 55 102738.0 56 93957.5 57 86188.0 58 81097.0 59 72956.5 60 57677.0 61 46404.5 62 39760.0 63 29167.0 64 18959.0 65 14501.0 66 11354.0 67 8669.0 68 6619.5 69 4795.0 70 3355.5 71 2367.5 72 1800.0 73 1595.5 74 1294.0 75 941.0 76 676.0 77 457.0 78 308.5 79 215.5 80 134.0 81 84.0 82 50.5 83 24.0 84 12.0 85 8.0 86 9.0 87 7.5 88 2.5 89 1.5 90 2.5 91 1.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0453457028487049 3 3.229750915149922E-5 4 0.0 5 0.10180174884552554 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011950078386054712 26-27 0.010528987983388746 28-29 0.0 30-31 0.0 32-33 5.329089009997371E-4 34-35 0.014727664173083644 36-37 0.0030359658602409265 38-39 0.011837037104024464 40-41 0.007024708240451079 42-43 0.005539022819482115 44-45 0.002599949486695687 46-47 9.689252745449765E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0993380948474492E-4 68-69 1.4533879118174648E-4 70-71 0.0 72-73 3.229750915149922E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.614875457574961E-5 82-83 8.074377287874805E-4 84-85 0.0039241473619071555 86-87 0.0013564953843629671 88-89 6.459501830299844E-5 90-91 8.2358648336323E-4 92-93 2.5838007321199376E-4 94-95 0.0 96-97 1.614875457574961E-5 98-99 0.0 100-101 7.589914650602316E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3096214.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.672554877438444 #Duplication Level Percentage of deduplicated Percentage of total 1 70.52841893255788 30.80156246251103 2 14.791718360149666 12.919842636305 3 5.780695975951134 7.573732867185404 4 2.8435731164458016 4.967444119039517 5 1.5988878368695714 3.491375839927761 6 0.9956320654502044 2.6089077609666864 7 0.6763377272111842 2.0676177555118707 8 0.4846434438162318 1.6932493916844111 9 0.34148974396189413 1.3422356624932401 >10 1.7383852969493698 13.516577855672221 >50 0.130682260483932 3.945077919403571 >100 0.07630412966279382 6.362476596295082 >500 0.007835182748717097 2.283874155991917 >1k 0.004878510013138593 4.662816926071634 >5k 3.695840919044388E-4 0.9760592938162591 >10k+ 1.4783363676177554E-4 0.7871487571244762 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13712 0.44286344548535733 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10672 0.34467901766479964 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7449 0.24058414566951766 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6065 0.19588439300384275 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6051 0.19543222787572176 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5408 0.17466492949130777 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5263 0.16998179066434038 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4807 0.15525412649125672 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4676 0.15102315279241035 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4618 0.1491498972616234 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3880 0.12531433550781698 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3854 0.12447460026987799 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3852 0.12441000525157499 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3764 0.12156782444624305 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3554 0.11478534752442822 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3504 0.11317047206685325 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3483 0.11249222437467177 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3395 0.10965004356933986 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3287 0.10616191258097793 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.459501830299844E-5 2 0.0 0.0 0.0 0.0 6.459501830299844E-5 3 0.0 0.0 0.0 0.0 6.459501830299844E-5 4 0.0 0.0 0.0 0.0 6.459501830299844E-5 5 0.0 0.0 0.0 0.0 9.689252745449766E-5 6 0.0 0.0 0.0 0.0 9.689252745449766E-5 7 0.0 0.0 0.0 0.0 9.689252745449766E-5 8 0.0 0.0 0.0 0.0 9.689252745449766E-5 9 0.0 0.0 0.0 0.0 9.689252745449766E-5 10-11 0.0 0.0 0.0 0.0 9.689252745449766E-5 12-13 0.0 0.0 0.0 0.0 2.0993380948474492E-4 14-15 0.0 0.0 0.0 3.229750915149922E-5 2.4223131863624416E-4 16-17 0.0 0.0 0.0 9.689252745449766E-5 2.5838007321199376E-4 18-19 0.0 0.0 0.0 1.614875457574961E-4 2.5838007321199376E-4 20-21 0.0 1.614875457574961E-5 0.0 2.4223131863624416E-4 2.7452882778774337E-4 22-23 0.0 4.844626372724883E-5 0.0 5.006113918482379E-4 2.9067758236349297E-4 24-25 0.0 6.459501830299844E-5 0.0 9.204790108177277E-4 3.229750915149922E-4 26-27 0.0 6.459501830299844E-5 0.0 0.0018409580216354553 3.391238460907418E-4 28-29 0.0 6.459501830299844E-5 0.0 0.006669435639784589 3.552726006664914E-4 30-31 0.0 6.459501830299844E-5 0.0 0.022333727578261707 3.552726006664914E-4 32-33 0.0 6.459501830299844E-5 0.0 0.05224122105254998 3.552726006664914E-4 34-35 0.0 6.459501830299844E-5 0.0 0.09794219650192137 4.1986761896948985E-4 36-37 0.0 6.459501830299844E-5 0.0 0.17363140919845982 4.6831388269673866E-4 38-39 0.0 6.459501830299844E-5 0.0 0.28416963426946584 4.8446263727248826E-4 40-41 0.0 1.2919003660599688E-4 0.0 0.4058020537340119 4.8446263727248826E-4 42-43 0.0 1.2919003660599688E-4 0.0 0.5322952483258586 5.006113918482379E-4 44-45 0.0 1.2919003660599688E-4 0.0 0.688792828919448 5.490576555754867E-4 46-47 0.0 1.4533879118174648E-4 0.0 0.8692228637942985 6.136526738784851E-4 48-49 0.0 1.614875457574961E-4 0.0 1.0580018047848114 6.62098937605734E-4 50-51 0.0 1.614875457574961E-4 0.0 1.2691144733535862 7.105452013329828E-4 52-53 0.0 1.614875457574961E-4 0.0 1.4701018728033657 7.42842710484482E-4 54-55 0.0 1.614875457574961E-4 0.0 1.6888206047773182 7.42842710484482E-4 56-57 0.0 1.9378505490899532E-4 0.0 1.9413871263420421 7.42842710484482E-4 58-59 0.0 2.0993380948474492E-4 0.0 2.2088105021164557 7.42842710484482E-4 60-61 0.0 2.2608256406049453E-4 0.0 2.500957621146342 7.42842710484482E-4 62-63 0.0 2.2608256406049453E-4 0.0 2.782511157174536 8.074377287874805E-4 64-65 0.0 2.5838007321199376E-4 0.0 3.0772259281819667 8.235864833632302E-4 66-67 0.0 2.5838007321199376E-4 0.0 3.4221633259199784 8.558839925147292E-4 68-69 0.0 2.5838007321199376E-4 0.0 3.7986553900990048 8.720327470904789E-4 70-71 0.0 2.5838007321199376E-4 0.0 4.194719744823839 8.881815016662286E-4 72-73 0.0 2.5838007321199376E-4 0.0 4.61147065416021 9.366277653934773E-4 74-75 0.0 2.5838007321199376E-4 0.0 5.034228900198759 9.689252745449765E-4 76-77 0.0 2.5838007321199376E-4 0.0 5.490447365718261 0.0010012227836964757 78-79 0.0 2.5838007321199376E-4 0.0 5.986569403794441 0.001033520292847975 80-81 0.0 2.5838007321199376E-4 0.0 6.50723754882576 0.0010658178019994741 82-83 0.0 2.5838007321199376E-4 0.0 7.05540702289958 0.0010981153111509735 84-85 0.0 2.5838007321199376E-4 0.0 7.614073187447637 0.0010981153111509735 86-87 0.0 2.5838007321199376E-4 3.229750915149922E-5 8.186853363494901 0.001114264065726723 88-89 0.0 2.5838007321199376E-4 3.229750915149922E-5 8.790025495653724 0.0011304128203024726 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7285 0.0 53.276615 1 GTATCAA 13095 0.0 41.843018 1 TATCAAC 17290 0.0 31.608294 2 ATCAACG 17400 0.0 31.34214 3 TCAACGC 17565 0.0 31.101864 4 TAAGGTG 2285 0.0 30.37954 5 CAACGCA 18105 0.0 30.121693 5 AACGCAG 18825 0.0 29.039673 6 AGGTAAG 2355 0.0 26.457962 2 GTAAGGT 2565 0.0 26.321796 4 GGTAAGG 2465 0.0 25.653652 3 ACGCAGA 21335 0.0 25.467411 7 CGCAGAG 21520 0.0 25.204334 8 GTACATG 15565 0.0 24.202045 1 TACATGG 15370 0.0 24.044912 2 AAGGTAA 2505 0.0 23.35465 1 GGACCGA 1055 0.0 22.959854 6 GCAGAGT 23470 0.0 22.867395 9 TAAGGTA 1235 0.0 22.71431 4 ACATGGG 15905 0.0 22.629591 3 >>END_MODULE