FastQCFastQC Report
Thu 26 May 2016
SRR938660_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938660_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103173
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132260.42620891584194626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101750.3278901949714051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69810.22496328757694142No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA60360.194510586422349No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA60040.19347938384356916No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA54080.17427323581379445No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48020.15474483697815106No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA47950.15451926141404299No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC47100.151780129564159No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT41160.13263843169555806No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG40190.12951259887863165No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA40060.12909367283100234No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG38610.12442103614590613No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA37540.12097295252311102No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG36850.11874942196261698No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT35490.11436681100280262No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34700.11182102963643986No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA34630.11159545407233176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73150.053.7993351
GTATCAA135100.044.0299721
TATCAAC178950.033.223782
ATCAACG181300.032.7931373
TCAACGC184700.032.2152024
CAACGCA188450.031.5741465
AACGCAG195450.030.4374336
TAAGGTG22050.027.3727595
ACGCAGA220400.026.9654757
CGCAGAG222100.026.6949068
GTGTAAG11100.026.2146551
GCAGAGT232250.024.9334499
GTACATG159300.024.914081
TAAGGTA11900.024.7609984
TGGTATC32450.024.7511522
TACATGG158200.024.6638072
GTGGTAT36850.023.6892281
ACATGGG161700.023.571533
AGGTAAG21900.023.0030382
CGACGAG5350.022.6583894-95
Produced by FastQC (version 0.11.5)