FastQCFastQC Report
Thu 26 May 2016
SRR938660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938660_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103173
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130500.42053730165865716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107290.3457428896165312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74120.23885229730988253No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA60310.19434946101941467No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA60260.1941883356164803No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA54340.17511108790905308No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC54020.17407988533027324No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48500.15629164084632086No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46920.15120007811359534No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA45870.14781644465197397No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38500.12406656025945056No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG38410.12377653453416873No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT38110.12280978211656263No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG38080.12271310687480201No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA37620.12123075316780597No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT35540.11452793640573697No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA34800.11214328044230858No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT34630.11159545407233176No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA33460.10782511964366795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70750.053.1844561
GTATCAA129600.043.1476821
ATCAACG172550.032.1790773
TATCAAC174700.031.947732
TCAACGC176050.031.6202744
CAACGCA179450.030.968235
AACGCAG186750.029.732266
TAAGGTG22550.029.6993245
GTAAGGT24100.027.0008564
AGGTAAG23050.026.7896752
GGTAAGG24400.026.6688793
ACGCAGA211000.026.2701427
CGCAGAG213200.025.9099488
AAGGTAA22850.025.5743681
GTACATG154900.024.6291891
TACATGG153400.024.462272
GCAGAGT229800.023.6455829
GGACCGA10700.023.5269766
ACATGGG156150.023.1177523
ACCGAGT11350.022.1796178
Produced by FastQC (version 0.11.5)