##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938660_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3103173 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83258587258912 31.0 31.0 34.0 30.0 34.0 2 32.08542739963257 33.0 31.0 34.0 30.0 34.0 3 32.13413013067593 33.0 31.0 34.0 30.0 34.0 4 35.79029399907772 37.0 35.0 37.0 35.0 37.0 5 35.6872871734834 37.0 35.0 37.0 33.0 37.0 6 35.725174200729384 37.0 35.0 37.0 33.0 37.0 7 35.6500088780097 37.0 35.0 37.0 33.0 37.0 8 35.61858265717058 37.0 35.0 37.0 33.0 37.0 9 37.16358127632588 39.0 37.0 39.0 33.0 39.0 10-11 37.09771321160632 39.0 37.0 39.0 33.0 39.0 12-13 36.993357444138624 39.0 37.0 39.0 33.0 39.0 14-15 38.00474079273054 40.0 37.0 41.0 33.0 41.0 16-17 38.0908652208562 40.0 37.0 41.0 33.0 41.0 18-19 38.25393266827212 40.0 38.0 41.0 33.0 41.0 20-21 38.214548463782066 40.0 38.0 41.0 33.0 41.0 22-23 38.09800372070781 40.0 37.0 41.0 33.0 41.0 24-25 37.97604774210139 40.0 37.0 41.0 33.0 41.0 26-27 37.762834041157234 40.0 37.0 41.0 32.0 41.0 28-29 37.62223843143776 40.0 37.0 41.0 32.0 41.0 30-31 37.34368580159727 39.0 36.0 41.0 31.5 41.0 32-33 37.19929133825281 39.0 36.0 41.0 31.0 41.0 34-35 37.05629673240905 39.0 36.0 41.0 31.0 41.0 36-37 36.90730262218703 39.0 35.5 41.0 31.0 41.0 38-39 36.67544155611047 39.0 35.0 41.0 30.0 41.0 40-41 36.442378494528015 39.0 35.0 40.0 30.0 41.0 42-43 36.14743602757565 39.0 35.0 40.0 30.0 41.0 44-45 35.821445339979434 38.0 35.0 40.0 29.0 41.0 46-47 35.35589298437438 38.0 34.5 40.0 27.0 41.0 48-49 34.896496102537625 38.0 34.0 40.0 26.0 41.0 50-51 34.48038491569758 38.0 33.5 40.0 24.0 41.0 52-53 33.90215837144755 37.5 33.0 40.0 23.0 41.0 54-55 33.49228708808694 37.0 32.5 40.0 22.5 41.0 56-57 33.113851531964215 36.5 32.0 39.5 21.0 41.0 58-59 32.601506425842196 36.0 31.0 39.0 19.0 40.0 60-61 32.14771106863846 35.5 31.0 39.0 17.5 40.0 62-63 31.7025684678231 35.0 30.5 38.5 13.5 40.0 64-65 31.103255925467256 35.0 29.5 38.0 8.5 40.0 66-67 30.499668726171567 34.0 29.0 38.0 7.5 40.0 68-69 29.822817483910825 34.0 28.0 37.0 4.0 39.0 70-71 29.096702471953705 34.0 26.5 36.0 2.0 39.0 72-73 28.57431151920953 33.0 26.0 36.0 2.0 39.0 74-75 27.923865024605462 33.0 26.0 35.0 2.0 38.0 76-77 26.767303015333013 31.5 25.0 34.0 2.0 36.0 78-79 27.307364107640794 32.0 26.0 35.0 2.0 37.0 80-81 26.9872775381843 32.0 25.0 35.0 2.0 36.0 82-83 26.375946813148992 32.0 24.0 35.0 2.0 36.0 84-85 25.673427971949998 31.5 22.0 34.0 2.0 35.5 86-87 24.85795200589848 31.0 19.0 34.0 2.0 35.0 88-89 23.9454687186309 30.5 14.0 34.0 2.0 35.0 90-91 23.165212348779782 30.0 4.5 34.0 2.0 35.0 92-93 22.188322243071852 29.0 2.0 34.0 2.0 35.0 94-95 21.098567981868882 29.0 2.0 33.5 2.0 35.0 96-97 19.79703194117763 27.0 2.0 33.0 2.0 34.5 98-99 18.07422515599356 24.5 2.0 32.5 2.0 34.0 100-101 13.648488498707614 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.253543371433107 1101 2 0.14824569256793296 1101 3 0.2545600531054859 1101 4 0.1578474248203392 1101 5 0.1638683075174896 1101 6 0.15623891343668106 1101 7 0.17287022748143244 1101 8 0.20444118002621536 1101 9 0.229032731926047 1101 10-11 0.21018311335713236 1101 12-13 0.26515121921337226 1101 14-15 0.3250953925255615 1101 16-17 0.32251475394779305 1101 18-19 0.3463358133698975 1101 20-21 0.3287365012088088 1101 22-23 0.21815399110399625 1101 24-25 0.24160433099302026 1101 26-27 0.29249432346656334 1101 28-29 0.1885778529349622 1101 30-31 0.3906562267401199 1101 32-33 0.30965243116305174 1101 34-35 0.23401147012595658 1101 36-37 0.2582599328203301 1101 38-39 0.30710942403158015 1101 40-41 0.3657901666683543 1101 42-43 0.3998654320590447 1101 44-45 0.36423705753372104 1101 46-47 0.43940795702474134 1101 48-49 0.5048581708399951 1101 50-51 0.638097323558533 1101 52-53 0.6936475191717406 1101 54-55 0.7073188612113981 1101 56-57 0.9538176464326327 1101 58-59 0.7457116166244759 1101 60-61 0.8541234862023117 1101 62-63 0.907178023828223 1101 64-65 0.8547406181485577 1101 66-67 1.0230367289982603 1101 68-69 1.1100660334119148 1101 70-71 1.2782502218420646 1101 72-73 1.4197189918557278 1101 74-75 1.2582727133764955 1101 76-77 1.2835934691123256 1101 78-79 1.3143822181411515 1101 80-81 1.4358238064193003 1101 82-83 1.5753526167969945 1101 84-85 1.7420582952230461 1101 86-87 1.7614136141503778 1101 88-89 1.401098253442843 1101 90-91 1.745398741611865 1101 92-93 2.0862133202591053 1101 94-95 1.991204727015603 1101 96-97 1.9141338396421936 1101 98-99 2.207745508505596 1101 100-101 1.8939448789183686 1102 1 0.23537157314794044 1102 2 0.22516211708710188 1102 3 0.2052233941207433 1102 4 0.09500835867470414 1102 5 0.18849578207411355 1102 6 0.2231008488035755 1102 7 0.17998733621639218 1102 8 0.25553967083892104 1102 9 0.41430539239946995 1102 10-11 0.29563821944875457 1102 12-13 0.31908301473114165 1102 14-15 0.37281306285187554 1102 16-17 0.2674616842808817 1102 18-19 0.26774523111847515 1102 20-21 0.2095435680987805 1102 22-23 0.29018422494929297 1102 24-25 0.20246291685160145 1102 26-27 0.28149957436954764 1102 28-29 0.27914250989664424 1102 30-31 0.21747960936180988 1102 32-33 0.19644400061286404 1102 34-35 0.2784079622078437 1102 36-37 0.3193983004360277 1102 38-39 0.2893378251900671 1102 40-41 0.27588425826930063 1102 42-43 0.3112946152466165 1102 44-45 0.20656265168972965 1102 46-47 0.26921613564609714 1102 48-49 0.4194925281113413 1102 50-51 0.4302274104453474 1102 52-53 0.2769883077853237 1102 54-55 0.15906202501762579 1102 56-57 0.3257064088799666 1102 58-59 0.5020220640367157 1102 60-61 0.41780887576157966 1102 62-63 0.5721618846375449 1102 64-65 0.5589055551902042 1102 66-67 0.5927465814194974 1102 68-69 0.8565280556179182 1102 70-71 0.9604861975652881 1102 72-73 1.0074926901041579 1102 74-75 1.0171704412456428 1102 76-77 1.00797175838197 1102 78-79 0.9713128852277002 1102 80-81 1.2035518664241671 1102 82-83 1.3203551933418503 1102 84-85 1.3050428884391003 1102 86-87 1.3521011341305282 1102 88-89 1.3296212013918627 1102 90-91 1.4927819382451801 1102 92-93 1.9300260937187836 1102 94-95 1.7098657109694564 1102 96-97 1.8882094445338105 1102 98-99 1.7074595239553219 1102 100-101 1.5885202380839498 1103 1 0.18313203502357922 1103 2 0.2685280867942481 1103 3 0.2823065958110931 1103 4 0.13647007800410904 1103 5 0.21680595178547435 1103 6 0.2988787063985754 1103 7 0.20669229297652691 1103 8 0.2619106368822415 1103 9 0.36452134231859645 1103 10-11 0.3024357087537979 1103 12-13 0.37472282179518857 1103 14-15 0.46685659205888186 1103 16-17 0.3880559401017152 1103 18-19 0.36759643297732936 1103 20-21 0.376469225011121 1103 22-23 0.38496273771063017 1103 24-25 0.45820864801612515 1103 26-27 0.48819776329468567 1103 28-29 0.4322373394037342 1103 30-31 0.3997221350890072 1103 32-33 0.5139434546409163 1103 34-35 0.5585924508399884 1103 36-37 0.5348790162634529 1103 38-39 0.39573819611841543 1103 40-41 0.5752435865187167 1103 42-43 0.4590077410773503 1103 44-45 0.6901251546690261 1103 46-47 0.8233863913161699 1103 48-49 0.936763255922429 1103 50-51 0.9116208527956999 1103 52-53 0.9825021255870041 1103 54-55 0.902660988945776 1103 56-57 1.0962966184112517 1103 58-59 0.9595694194816389 1103 60-61 0.9782458916618353 1103 62-63 1.127848528888876 1103 64-65 1.2661323946168075 1103 66-67 1.1641813291804581 1103 68-69 1.150029548310762 1103 70-71 1.283347262393498 1103 72-73 1.3583179114583679 1103 74-75 1.1743868046623085 1103 76-77 1.2148103202974063 1103 78-79 1.3690228239125553 1103 80-81 1.4666788888760856 1103 82-83 1.6446263623647859 1103 84-85 1.71685206764095 1103 86-87 1.8033904593535475 1103 88-89 1.7619662700734686 1103 90-91 1.905319726709596 1103 92-93 2.073634037084034 1103 94-95 1.8557175436060511 1103 96-97 2.2701297082410328 1103 98-99 2.122890963736001 1103 100-101 2.152142182719624 1104 1 0.17877181602129255 1104 2 0.21591656300109463 1104 3 0.22526694474031217 1104 4 0.1344681166607984 1104 5 0.04944442056682874 1104 6 0.13468855510401312 1104 7 0.11668933039867824 1104 8 0.1399859615028376 1104 9 0.16214351184456177 1104 10-11 0.1846932946158759 1104 12-13 0.26359549872925214 1104 14-15 0.3374000004247222 1104 16-17 0.3153171650857658 1104 18-19 0.34289876442708334 1104 20-21 0.32681133040121324 1104 22-23 0.3164559810276302 1104 24-25 0.20714902505810073 1104 26-27 0.3927884769459311 1104 28-29 0.3975000987340209 1104 30-31 0.31921750932960435 1104 32-33 0.4196842298411738 1104 34-35 0.35485245652143504 1104 36-37 0.2896224410288184 1104 38-39 0.4382728934391764 1104 40-41 0.3760130824768453 1104 42-43 0.4040585225048545 1104 44-45 0.36108969588705975 1104 46-47 0.4384598833018529 1104 48-49 0.4944035461687477 1104 50-51 0.49104243676193704 1104 52-53 0.3643044086759133 1104 54-55 0.47547293988833417 1104 56-57 0.6603246022056766 1104 58-59 0.7066006220013819 1104 60-61 0.7673027967656694 1104 62-63 0.6312731368677333 1104 64-65 0.8129458844751163 1104 66-67 0.8112749057684283 1104 68-69 0.9175800930112885 1104 70-71 0.9678056221814373 1104 72-73 1.1871519936443882 1104 74-75 1.2297233542669552 1104 76-77 1.2711838990787498 1104 78-79 1.27020155925484 1104 80-81 1.5445281106726334 1104 82-83 1.5991747190226029 1104 84-85 1.9416095462460348 1104 86-87 1.821961468688837 1104 88-89 1.6951383709676513 1104 90-91 1.643192324184625 1104 92-93 1.8354757171271494 1104 94-95 1.89080397243551 1104 96-97 1.9332584595385889 1104 98-99 2.090109862190154 1104 100-101 2.1632852960462223 1105 1 0.1034519880741307 1105 2 0.19516786633512595 1105 3 0.17851377148279823 1105 4 0.11797420477152087 1105 5 0.08588012951658897 1105 6 0.05444045422952115 1105 7 0.12357960526486522 1105 8 0.11779616388953684 1105 9 0.2517715721671152 1105 10-11 0.2786721936241108 1105 12-13 0.22858124732243112 1105 14-15 0.296713774461729 1105 16-17 0.21944314525364916 1105 18-19 0.2857350545409858 1105 20-21 0.2290324849680232 1105 22-23 0.2582202440392507 1105 24-25 0.24005283820536505 1105 26-27 0.2510934686272961 1105 28-29 0.22417470772796122 1105 30-31 0.3687415718569014 1105 32-33 0.40526877721679 1105 34-35 0.3287436243440425 1105 36-37 0.22426228680938465 1105 38-39 0.3488941757348911 1105 40-41 0.2892638332232025 1105 42-43 0.42497488965832275 1105 44-45 0.4707912108495478 1105 46-47 0.6021390181127799 1105 48-49 0.6439683687601558 1105 50-51 0.5287467237829375 1105 52-53 0.45017087398209554 1105 54-55 0.49535212359730707 1105 56-57 0.6501393216399833 1105 58-59 0.5835242286847659 1105 60-61 0.5681022482469871 1105 62-63 0.6490135279983065 1105 64-65 0.8413223021472156 1105 66-67 0.8985673886935714 1105 68-69 0.9764930597511459 1105 70-71 0.9339381129686259 1105 72-73 1.0578972819458414 1105 74-75 1.1106531631214303 1105 76-77 1.1186045739255661 1105 78-79 1.148661677706908 1105 80-81 1.2875528605436948 1105 82-83 1.3653530794975488 1105 84-85 1.5905354320320306 1105 86-87 1.7625378318972587 1105 88-89 1.6474718378683413 1105 90-91 1.8031517231385479 1105 92-93 2.025039246527772 1105 94-95 1.8329481248571078 1105 96-97 1.9854960011478227 1105 98-99 2.119123170479707 1105 100-101 2.016027696533378 1106 1 0.020634039385843295 1106 2 0.009229536310400022 1106 3 -0.010119233125521987 1106 4 0.03432439612489446 1106 5 0.07615429738968515 1106 6 0.025300885946926144 1106 7 0.022490434860259256 1106 8 0.016082125287852023 1106 9 -0.17448185693336882 1106 10-11 0.04497332314733882 1106 12-13 0.03123212107013984 1106 14-15 -0.0026716680001257487 1106 16-17 0.00861313832204047 1106 18-19 0.12194167302747871 1106 20-21 0.13938275151024015 1106 22-23 0.1783360504239866 1106 24-25 0.026328227785874958 1106 26-27 -0.22709815302246028 1106 28-29 0.025839728989943467 1106 30-31 0.1546422407261403 1106 32-33 -0.11410269302310638 1106 34-35 -0.16046043727698844 1106 36-37 -0.26416591719172544 1106 38-39 -0.14330653931541804 1106 40-41 -0.09142287890676215 1106 42-43 0.04361392494546834 1106 44-45 0.0880132813099479 1106 46-47 -0.3303050885503751 1106 48-49 -0.10698472764083533 1106 50-51 0.09695312293157343 1106 52-53 -0.11308023583187321 1106 54-55 -0.2795843218124787 1106 56-57 -0.24700523757990567 1106 58-59 0.05376563157503966 1106 60-61 0.05108815946008605 1106 62-63 0.11258253311204314 1106 64-65 0.3605128631449368 1106 66-67 0.2834358126732077 1106 68-69 0.14759316145628532 1106 70-71 0.3015954111355299 1106 72-73 0.4449028597120126 1106 74-75 0.38830647272059693 1106 76-77 0.32235643028908 1106 78-79 0.5827840749050779 1106 80-81 0.6258256149555912 1106 82-83 0.9902459131558068 1106 84-85 0.992917349961413 1106 86-87 0.9964479763399119 1106 88-89 1.047333483729794 1106 90-91 1.1810449919132893 1106 92-93 1.17870488042308 1106 94-95 0.8615778883370417 1106 96-97 1.0630065186872883 1106 98-99 1.352051633415165 1106 100-101 1.5716184984023336 1107 1 0.09958724672866026 1107 2 0.1108896176409715 1107 3 0.14489230792669616 1107 4 0.1126042361379973 1107 5 0.12377471794864192 1107 6 0.03939145771789754 1107 7 0.04898429495361967 1107 8 0.14123192496995074 1107 9 0.25557924128006704 1107 10-11 0.12112598678208286 1107 12-13 0.18801451582594098 1107 14-15 0.2989581400055741 1107 16-17 0.18630679567396413 1107 18-19 0.15552385768765475 1107 20-21 0.2430208363139883 1107 22-23 0.23842880922853027 1107 24-25 0.24378826866844605 1107 26-27 0.2896828586456124 1107 28-29 0.23538263730167586 1107 30-31 0.2855328990758963 1107 32-33 0.2594239633811313 1107 34-35 0.28333167168525364 1107 36-37 0.2974714288509759 1107 38-39 0.3066254762821288 1107 40-41 0.3673296637182517 1107 42-43 0.34783796259219457 1107 44-45 0.4700221096716817 1107 46-47 0.6469898133297889 1107 48-49 0.6640171042191554 1107 50-51 0.5531244937678892 1107 52-53 0.4789474334438566 1107 54-55 0.6527496784114888 1107 56-57 0.5920100373913044 1107 58-59 0.736637719778436 1107 60-61 0.7505606431179217 1107 62-63 0.8224445753350835 1107 64-65 1.0282526763487034 1107 66-67 0.8980029020382219 1107 68-69 1.0423479161859461 1107 70-71 0.953754490458742 1107 72-73 1.2988532883438495 1107 74-75 1.322343321353138 1107 76-77 1.1876342846729244 1107 78-79 1.2761703458164426 1107 80-81 1.565228718587722 1107 82-83 1.7646295198474462 1107 84-85 1.7844654513287885 1107 86-87 2.052472012816658 1107 88-89 2.1020461984426717 1107 90-91 2.194290772868964 1107 92-93 2.2481369286130324 1107 94-95 2.134510297550495 1107 96-97 2.332083832037302 1107 98-99 2.2512715468698126 1107 100-101 1.9334931630766867 1108 1 0.04516055973851962 1108 2 0.03609033878651502 1108 3 0.0490471454578838 1108 4 0.07061723302884815 1108 5 0.041535843476012246 1108 6 -0.010237953599848026 1108 7 0.028146020821608886 1108 8 -0.011846873765513521 1108 9 0.03195389619166633 1108 10-11 0.07462011960763704 1108 12-13 0.025265566729018474 1108 14-15 0.04247427713374208 1108 16-17 0.03855676823574328 1108 18-19 -0.20837277658272058 1108 20-21 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0.48875039582410196 2316 96-97 0.2825078249907591 2316 98-99 0.22180477182448044 2316 100-101 -0.2868495073099524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 21.0 4 53.0 5 102.0 6 248.0 7 661.0 8 1611.0 9 2875.0 10 5001.0 11 7551.0 12 10719.0 13 14912.0 14 19576.0 15 26472.0 16 33781.0 17 42683.0 18 40969.0 19 34869.0 20 34328.0 21 36415.0 22 39900.0 23 44800.0 24 50581.0 25 58056.0 26 67786.0 27 79835.0 28 94602.0 29 112164.0 30 131593.0 31 156175.0 32 185770.0 33 225530.0 34 278806.0 35 348327.0 36 410952.0 37 372404.0 38 130041.0 39 2995.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62841092527553 19.295000232079587 15.179971273704352 22.89661756894053 2 15.837373712632669 20.903629057728974 38.931734885801546 24.327262343836818 3 19.062917019940258 24.570408031403243 30.35613523039239 26.01053971826411 4 13.026408775791745 17.224692274649207 36.52016822781069 33.22873072174835 5 13.31154696031114 38.08356183674528 33.94236783565672 14.662523367286862 6 32.0458446886461 36.49371143664887 17.020481938970207 14.43996193573481 7 28.696563162930328 33.436936967420124 20.03001444005861 17.836485429590937 8 27.28959271352023 34.045228559680226 19.64970037110415 19.015478355695397 9 27.12370942330851 16.180179796962264 19.964378327044596 36.73173245268463 10-11 24.99744616236349 26.62900199247673 27.070485596516857 21.30306624864292 12-13 26.345292382989925 24.11038636904871 27.316024598048514 22.228296649912846 14-15 22.674678466202174 25.611930111534225 26.018642853621117 25.694748568642485 16-17 21.79420870186741 28.713046291650514 27.227695652159902 22.265049354322173 18-19 21.97212337178752 26.548842104516897 29.914719546734908 21.564314976960677 20-21 22.548775076349273 25.69449076799779 30.14949537135055 21.60723878430239 22-23 22.345128679580544 25.92159702343376 30.132287178317164 21.600987118668538 24-25 22.01378545376501 26.193635063481146 30.121819094680653 21.670760388073187 26-27 21.35123973001503 26.52676266998742 29.91283836948726 22.20915923051029 28-29 21.604030595869247 26.973501883883678 29.739485247959824 21.68298227228725 30-31 21.608576125146744 26.40951696859956 29.52761576618513 22.45429114006857 32-33 21.1234785898464 26.585481824930056 29.510414446151838 22.7806251390717 34-35 22.40084592910316 26.61234689732265 29.264544764419796 21.722262409154393 36-37 22.549328276947953 25.850920632301676 29.277160965074394 22.32259012567597 38-39 21.72776826481259 26.336759612257772 29.270416268234033 22.665055854695606 40-41 22.382777765781455 26.16385893658089 29.428842243610053 22.024521054027602 42-43 22.02155873415229 26.332016494299165 29.017907281361992 22.62851749018655 44-45 21.925791033386655 26.590944831648812 29.35603301928746 22.12723111567707 46-47 22.636730387548987 26.970832075210854 28.186089768233703 22.206347769006456 48-49 22.07356792547499 27.071677924498573 28.02183442560244 22.832919724424 50-51 22.441916709123213 27.211164185818838 28.07131603684358 22.275603068214373 52-53 22.918831789268598 26.700912259806337 28.113788693057074 22.266467257867994 54-55 22.017012909045032 27.153771317293625 28.354510045040993 22.47470572862035 56-57 22.320899015443064 27.265193926538085 27.846953400109083 22.566953657909767 58-59 22.10916052698319 26.78548698380657 27.83684635049351 23.268506138716727 60-61 21.478499587357845 27.175700484632987 28.43681290085986 22.90898702714931 62-63 21.82253454770327 27.121368998763522 28.482250264487348 22.573846189045856 64-65 21.43542156219507 27.257351510003314 28.116762461120427 23.190464466681192 66-67 21.419472408770414 27.202190670155584 28.18230448961704 23.196032431456967 68-69 22.31277015943022 26.570854177109705 28.22831808186825 22.888057581591834 70-71 21.893839593809172 26.87725656199527 27.723632463814447 23.505271380381107 72-73 21.74894229024735 27.010051344320175 27.709933110270896 23.53107325516158 74-75 21.834844528487455 27.46127914879383 27.402452264182497 23.301424058536213 76-77 21.826498232615453 26.83532307093417 28.077342126913322 23.26083656953705 78-79 21.895379342369893 27.101631136904064 27.82551923466723 23.177470286058817 80-81 22.068968517510886 27.193744975902863 27.566347890439115 23.170938616147136 82-83 21.9367377493679 27.151493999960042 27.660122984867613 23.251645265804445 84-85 21.74862822688748 27.27150851455659 27.620508412881108 23.359354845674822 86-87 21.98986320727735 27.363555724564765 27.111030187210822 23.535550880947067 88-89 21.70555750517293 27.31834480385077 26.924119280491293 24.05197841048501 90-91 21.51143005748382 27.196279632139635 27.270720503851916 24.021569806524628 92-93 21.68828040471681 26.77435805304758 27.49689630642338 24.040465235812235 94-95 21.288856026441344 26.530897996097863 27.69203303524573 24.488212942215068 96-97 21.592921694317944 26.05382492494776 27.71902427580553 24.634229104928764 98-99 21.23747209710835 26.403265302965707 27.28326780363196 25.075994796293987 100-101 21.2887593511217 25.953665775051675 27.685169087550943 25.07240578627568 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 643.0 1 469.0 2 387.0 3 513.0 4 516.5 5 499.0 6 842.0 7 1842.0 8 2917.5 9 3308.0 10 2929.0 11 2540.5 12 2606.0 13 2770.0 14 3040.5 15 3490.5 16 3877.5 17 4201.0 18 4669.0 19 5307.0 20 6078.0 21 7046.0 22 8382.0 23 10109.0 24 12218.0 25 15221.5 26 19254.0 27 23709.5 28 28171.5 29 32486.0 30 37455.0 31 46101.0 32 59667.0 33 64609.5 34 62364.0 35 64875.0 36 70284.5 37 75753.5 38 79686.0 39 82378.0 40 86327.0 41 92505.5 42 96724.0 43 99358.0 44 101376.5 45 106677.5 46 109793.5 47 111425.0 48 113326.0 49 110626.5 50 108761.0 51 106188.5 52 102337.5 53 103822.0 54 107408.5 55 102595.0 56 94015.0 57 86572.0 58 81541.0 59 73083.5 60 58215.0 61 47255.5 62 40289.5 63 29773.5 64 19667.5 65 14878.0 66 11431.0 67 8819.5 68 6799.0 69 4895.0 70 3501.0 71 2459.5 72 1870.5 73 1650.0 74 1337.5 75 988.0 76 678.0 77 460.5 78 321.5 79 202.0 80 125.0 81 70.0 82 42.0 83 27.5 84 13.5 85 9.5 86 6.0 87 5.5 88 6.0 89 7.5 90 6.5 91 2.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025425588583040646 2 0.01060205151308032 3 0.0017401543516909952 4 0.0 5 3.2225080586870277E-4 6 0.0 7 0.0 8 3.222508058687028E-5 9 1.9335048352122166E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015145787875829031 26-27 0.008362408412292837 28-29 2.900257252818325E-4 30-31 0.0 32-33 2.4168810440152708E-4 34-35 0.010070337683396961 36-37 0.0010956527399535894 38-39 0.01256778142887941 40-41 0.011923279817142004 42-43 0.00520435051477955 44-45 5.478263699767947E-4 46-47 1.289003223474811E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.611254029343514E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.222508058687028E-5 66-67 4.189260476293136E-4 68-69 9.18414796725803E-4 70-71 3.222508058687028E-5 72-73 1.9335048352122166E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1278778205404598E-4 82-83 8.700771758454976E-4 84-85 0.0052688006759532904 86-87 0.0038831222107178686 88-89 0.0 90-91 0.001256778142887941 92-93 4.994887490964892E-4 94-95 8.056270146717569E-5 96-97 3.222508058687028E-5 98-99 0.0 100-101 8.056270146717569E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3103173.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.669044825533035 #Duplication Level Percentage of deduplicated Percentage of total 1 70.7884524910101 30.91264104960036 2 14.49288844148552 12.657811900053616 3 5.833989845461564 7.642942922194967 4 2.819770484439138 4.925467347307508 5 1.604430353659339 3.503197051669775 6 0.995095736465246 2.6072928192838583 7 0.6629378658445708 2.0264904366076837 8 0.4872245049900778 1.702130299880788 9 0.34389099111109017 1.3515651995334463 >10 1.7530828834061443 13.69441931255736 >50 0.12756768902604107 3.8558030825016543 >100 0.07748742587912263 6.4208320791947004 >500 0.007775478417607939 2.2819546758003044 >1k 0.004887443576598396 4.668490047854624 >5k 3.7026087701503E-4 0.9799993828202926 >10k+ 1.48104350806012E-4 0.7689623931392093 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13050 0.42053730165865716 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10729 0.3457428896165312 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7412 0.23885229730988253 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6031 0.19434946101941467 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6026 0.1941883356164803 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5434 0.17511108790905308 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5402 0.17407988533027324 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4850 0.15629164084632086 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4692 0.15120007811359534 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4587 0.14781644465197397 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3850 0.12406656025945056 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3841 0.12377653453416873 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3811 0.12280978211656263 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3808 0.12271310687480201 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3762 0.12123075316780597 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3554 0.11452793640573697 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3480 0.11214328044230858 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3463 0.11159545407233176 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3346 0.10782511964366795 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.667524176061083E-5 2 0.0 0.0 0.0 0.0 9.667524176061083E-5 3 0.0 0.0 0.0 0.0 9.667524176061083E-5 4 0.0 0.0 0.0 3.222508058687028E-5 9.667524176061083E-5 5 0.0 0.0 0.0 3.222508058687028E-5 9.667524176061083E-5 6 0.0 0.0 0.0 3.222508058687028E-5 9.667524176061083E-5 7 0.0 0.0 0.0 3.222508058687028E-5 9.667524176061083E-5 8 0.0 0.0 0.0 3.222508058687028E-5 9.667524176061083E-5 9 0.0 0.0 0.0 3.222508058687028E-5 9.667524176061083E-5 10-11 0.0 0.0 0.0 3.222508058687028E-5 9.667524176061083E-5 12-13 0.0 0.0 0.0 6.445016117374055E-5 2.900257252818325E-4 14-15 0.0 0.0 0.0 6.445016117374055E-5 3.0613826557526763E-4 16-17 0.0 0.0 0.0 6.445016117374055E-5 3.705884267490082E-4 18-19 0.0 0.0 0.0 9.667524176061083E-5 3.867009670424433E-4 20-21 0.0 3.222508058687028E-5 0.0 1.9335048352122166E-4 4.189260476293136E-4 22-23 1.611254029343514E-5 6.445016117374055E-5 0.0 5.639389102702298E-4 4.511511282161839E-4 24-25 3.222508058687028E-5 6.445016117374055E-5 0.0 0.0014501286264091626 4.511511282161839E-4 26-27 3.222508058687028E-5 6.445016117374055E-5 0.0 0.0027069067692971037 4.994887490964892E-4 28-29 3.222508058687028E-5 6.445016117374055E-5 0.0 0.006896167245590239 5.156012893899244E-4 30-31 3.222508058687028E-5 6.445016117374055E-5 0.0 0.021671366694670263 5.156012893899244E-4 32-33 3.222508058687028E-5 6.445016117374055E-5 0.0 0.051189540512243434 5.156012893899244E-4 34-35 3.222508058687028E-5 6.445016117374055E-5 0.0 0.09528956329537541 5.478263699767947E-4 36-37 3.222508058687028E-5 6.445016117374055E-5 0.0 0.16861773417079873 5.478263699767947E-4 38-39 3.222508058687028E-5 6.445016117374055E-5 0.0 0.28238838118274423 5.80051450563665E-4 40-41 3.222508058687028E-5 6.445016117374055E-5 0.0 0.3998487999218864 5.80051450563665E-4 42-43 3.222508058687028E-5 6.445016117374055E-5 0.0 0.5235931093754682 5.80051450563665E-4 44-45 3.222508058687028E-5 6.445016117374055E-5 0.0 0.6800297630844301 6.122765311505353E-4 46-47 3.222508058687028E-5 6.445016117374055E-5 0.0 0.8611991661438148 6.767266923242758E-4 48-49 3.222508058687028E-5 6.445016117374055E-5 0.0 1.049425861851724 7.411768534980164E-4 50-51 3.222508058687028E-5 6.445016117374055E-5 0.0 1.2599039757048671 8.056270146717569E-4 52-53 3.222508058687028E-5 6.445016117374055E-5 0.0 1.4560258161565598 8.056270146717569E-4 54-55 3.222508058687028E-5 6.445016117374055E-5 0.0 1.671466592420081 8.056270146717569E-4 56-57 3.222508058687028E-5 6.445016117374055E-5 0.0 1.9186168479810828 8.21739554965192E-4 58-59 3.222508058687028E-5 6.445016117374055E-5 0.0 2.183893711372199 8.378520952586272E-4 60-61 3.222508058687028E-5 6.445016117374055E-5 0.0 2.475997954351884 8.378520952586272E-4 62-63 3.222508058687028E-5 6.445016117374055E-5 0.0 2.7583863355346283 9.667524176061083E-4 64-65 3.222508058687028E-5 6.445016117374055E-5 0.0 3.0564522184228853 9.828649578995435E-4 66-67 3.222508058687028E-5 6.445016117374055E-5 0.0 3.4050792527519413 9.989774981929787E-4 68-69 3.222508058687028E-5 6.445016117374055E-5 0.0 3.782531621665953 9.989774981929787E-4 70-71 3.222508058687028E-5 6.445016117374055E-5 0.0 4.179576839576782 9.989774981929787E-4 72-73 6.445016117374055E-5 8.056270146717569E-5 0.0 4.593991375923933 0.0010312025787798489 74-75 6.445016117374055E-5 9.667524176061083E-5 0.0 5.002653735386328 0.0010634276593667192 76-77 6.445016117374055E-5 9.667524176061083E-5 0.0 5.450405117600598 0.0010956527399535894 78-79 6.445016117374055E-5 9.667524176061083E-5 0.0 5.95197238439494 0.0011117652802470246 80-81 6.445016117374055E-5 9.667524176061083E-5 0.0 6.472874699541405 0.0011278778205404598 82-83 6.445016117374055E-5 9.667524176061083E-5 0.0 7.010260143408054 0.00116010290112733 84-85 6.445016117374055E-5 9.667524176061083E-5 0.0 7.560906207936199 0.00116010290112733 86-87 6.445016117374055E-5 9.667524176061083E-5 0.0 8.127825938160715 0.0012084405220076353 88-89 6.445016117374055E-5 9.667524176061083E-5 0.0 8.720380719992086 0.001289003223474811 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7075 0.0 53.184456 1 GTATCAA 12960 0.0 43.147682 1 ATCAACG 17255 0.0 32.179077 3 TATCAAC 17470 0.0 31.94773 2 TCAACGC 17605 0.0 31.620274 4 CAACGCA 17945 0.0 30.96823 5 AACGCAG 18675 0.0 29.73226 6 TAAGGTG 2255 0.0 29.699324 5 GTAAGGT 2410 0.0 27.000856 4 AGGTAAG 2305 0.0 26.789675 2 GGTAAGG 2440 0.0 26.668879 3 ACGCAGA 21100 0.0 26.270142 7 CGCAGAG 21320 0.0 25.909948 8 AAGGTAA 2285 0.0 25.574368 1 GTACATG 15490 0.0 24.629189 1 TACATGG 15340 0.0 24.46227 2 GCAGAGT 22980 0.0 23.645582 9 GGACCGA 1070 0.0 23.526976 6 ACATGGG 15615 0.0 23.117752 3 ACCGAGT 1135 0.0 22.179617 8 >>END_MODULE