FastQCFastQC Report
Thu 26 May 2016
SRR938659_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938659_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410116
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128410.5327959318140704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101090.41944039208071315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70740.2935128433652156No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.1040613812779136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62900.057.158261
GTATCAA115050.043.567421
TATCAAC151500.032.895652
ATCAACG155200.032.142053
TCAACGC156150.031.855144
CAACGCA159700.031.0874715
TGGTATC27200.030.2459352
AACGCAG164950.030.1049586
GTGGTAT33750.026.0678861
ACGCAGA189300.026.0594887
CGCAGAG190700.025.8930998
GCAGAGT201150.023.7441259
TATACCG2653.092282E-1123.3285275
GTACATG150900.023.195111
TACATGG149750.023.181732
GAGTACT107250.022.41956912-13
ACATGGG153750.021.4960923
CAGAGTA194100.021.11733810-11
GTACTTT113650.020.94103214-15
AGTACTT111650.020.81249812-13
Produced by FastQC (version 0.11.5)