##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938659_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2410116 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.120482167663297 31.0 31.0 34.0 28.0 34.0 2 31.143285219466616 31.0 31.0 34.0 28.0 34.0 3 31.170768958838497 31.0 31.0 34.0 27.0 34.0 4 34.846186241658074 37.0 35.0 37.0 32.0 37.0 5 34.835283861855615 37.0 35.0 37.0 32.0 37.0 6 34.8656973357299 37.0 35.0 37.0 32.0 37.0 7 34.77226573326761 37.0 35.0 37.0 32.0 37.0 8 34.74886934902718 37.0 35.0 37.0 31.0 37.0 9 36.22212457823607 39.0 35.0 39.0 32.0 39.0 10-11 36.057751784561404 39.0 35.0 39.0 30.0 39.0 12-13 35.9104999510397 38.0 35.0 39.0 30.0 39.0 14-15 36.82905988757388 39.0 36.0 41.0 30.5 41.0 16-17 36.66582645814558 39.0 36.0 40.5 30.0 41.0 18-19 36.86134941222746 39.0 36.0 41.0 30.5 41.0 20-21 36.86459427679 39.0 36.0 41.0 30.0 41.0 22-23 36.763943519731 39.0 36.0 41.0 30.0 41.0 24-25 36.62776957623616 39.0 36.0 41.0 30.0 41.0 26-27 36.411825198455176 39.0 35.5 41.0 29.5 41.0 28-29 36.19963852362293 39.0 35.0 40.5 29.0 41.0 30-31 35.91875764486025 38.5 35.0 40.0 27.5 41.0 32-33 35.61937786397003 38.0 34.5 40.0 27.0 41.0 34-35 35.35465014961936 38.0 34.0 40.0 26.5 41.0 36-37 35.06110100094767 38.0 34.0 40.0 25.5 41.0 38-39 34.897616961175316 38.0 34.0 40.0 25.0 41.0 40-41 34.77085210836324 38.0 34.0 40.0 24.5 41.0 42-43 34.43695801363918 38.0 33.0 40.0 24.0 41.0 44-45 34.03063649218544 38.0 33.0 40.0 23.0 41.0 46-47 33.557908830944235 38.0 33.0 40.0 19.5 41.0 48-49 33.14525358945379 37.5 32.0 40.0 16.5 41.0 50-51 32.426709959188685 36.5 30.5 39.5 15.0 40.5 52-53 32.445525028670815 37.0 31.0 39.0 13.5 40.0 54-55 32.546913509557214 37.0 31.0 40.0 11.0 41.0 56-57 32.23054222286396 37.0 31.0 40.0 8.5 41.0 58-59 31.86572990677627 36.0 31.0 39.0 7.5 41.0 60-61 31.396995414328607 36.0 30.0 39.0 4.0 40.5 62-63 30.827991889187075 35.0 29.0 39.0 2.0 40.0 64-65 30.314215996242503 35.0 28.0 38.0 2.0 40.0 66-67 29.780649977013553 34.5 28.0 38.0 2.0 40.0 68-69 29.185359957777965 34.0 27.0 37.5 2.0 40.0 70-71 28.5688394251563 34.0 26.0 37.0 2.0 39.0 72-73 27.931032365247148 33.5 25.5 36.0 2.0 39.0 74-75 27.25328677125914 33.0 24.5 36.0 2.0 38.5 76-77 26.5012190284617 32.0 22.5 35.0 2.0 37.5 78-79 25.723836321571245 32.0 20.0 35.0 2.0 37.0 80-81 25.026613864228942 31.0 18.0 35.0 2.0 36.0 82-83 24.288909330505255 31.0 10.5 34.0 2.0 36.0 84-85 23.570811736862456 30.0 7.0 34.0 2.0 35.0 86-87 22.79414828995783 29.5 2.0 34.0 2.0 35.0 88-89 21.97312930166017 29.0 2.0 34.0 2.0 35.0 90-91 21.04576314998946 29.0 2.0 34.0 2.0 35.0 92-93 20.083715265157362 26.5 2.0 33.5 2.0 35.0 94-95 19.07456300858548 25.5 2.0 33.0 2.0 35.0 96-97 17.815673602432412 24.0 2.0 32.5 2.0 34.0 98-99 16.302051436528366 10.0 2.0 32.0 2.0 34.0 100-101 12.849252276653903 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4573856691358351 1101 2 0.32147430223918505 1101 3 0.3329158565430248 1101 4 0.2617421053501303 1101 5 0.29579903419739395 1101 6 0.4296752698174089 1101 7 0.34450056664695694 1101 8 0.5131630578013784 1101 9 0.6530771502093202 1101 10-11 0.6024192551977094 1101 12-13 0.7461297655144463 1101 14-15 0.8470497933636736 1101 16-17 0.6775914696975676 1101 18-19 0.747808486544379 1101 20-21 0.8006908609194383 1101 22-23 0.863593986549354 1101 24-25 0.8720705106478519 1101 26-27 0.7697652540757645 1101 28-29 0.9198914700029377 1101 30-31 0.8179766306252958 1101 32-33 0.8318457662644647 1101 34-35 0.8211267887481881 1101 36-37 0.7224597646646416 1101 38-39 0.7401755567267614 1101 40-41 0.6499554308934137 1101 42-43 0.9640397766902709 1101 44-45 1.002226870102973 1101 46-47 0.8782606647842854 1101 48-49 0.5732321598358112 1101 50-51 0.6397329543692152 1101 52-53 0.5773829458564776 1101 54-55 0.8197517964218548 1101 56-57 0.8041615360859993 1101 58-59 0.7489816668031644 1101 60-61 0.7266736721593894 1101 62-63 0.6404601076997629 1101 64-65 0.4868345033193542 1101 66-67 0.6588052573934426 1101 68-69 0.7096087011059815 1101 70-71 0.7875077704408255 1101 72-73 0.8212123746394333 1101 74-75 0.5959120063895327 1101 76-77 0.6464037681231716 1101 78-79 0.9041419057994666 1101 80-81 1.1636375599331465 1101 82-83 1.0551817184947332 1101 84-85 0.984160267310493 1101 86-87 0.94343902841036 1101 88-89 0.9220598958460791 1101 90-91 0.8774484635657593 1101 92-93 0.8703510007978394 1101 94-95 0.9049313504458958 1101 96-97 0.6780656151218842 1101 98-99 0.6948364236087485 1101 100-101 0.7594374870185323 1102 1 0.3332142574117398 1102 2 0.38514148406852655 1102 3 0.32739658620642587 1102 4 0.1589999920080416 1102 5 0.18157918039447196 1102 6 0.38747620718265097 1102 7 0.33237294479944524 1102 8 0.4046737854607869 1102 9 0.5288186539597248 1102 10-11 0.6275049495184319 1102 12-13 0.5670750627926893 1102 14-15 0.6114624397155097 1102 16-17 0.6778457984804689 1102 18-19 0.5899088184098744 1102 20-21 0.5040223161648143 1102 22-23 0.5429781415724051 1102 24-25 0.5571682499125856 1102 26-27 0.5341594343113556 1102 28-29 0.44005069828715904 1102 30-31 0.6729022048323259 1102 32-33 0.7186389258274417 1102 34-35 0.5802913858458609 1102 36-37 0.6260310695790423 1102 38-39 0.560484717284389 1102 40-41 0.6489522801123258 1102 42-43 0.654599604283888 1102 44-45 0.6346632328236623 1102 46-47 0.8737989992874873 1102 48-49 0.7913534003811051 1102 50-51 0.8334625537699125 1102 52-53 0.8169706268585202 1102 54-55 0.6680740553314877 1102 56-57 0.6839923402958519 1102 58-59 0.7222620359576162 1102 60-61 0.6068072787073042 1102 62-63 0.8421471343088065 1102 64-65 0.9238426987076522 1102 66-67 0.6074588571844401 1102 68-69 0.5274651207568688 1102 70-71 0.5896376690765379 1102 72-73 0.6127478636819994 1102 74-75 0.7014529953969451 1102 76-77 0.7482232051794178 1102 78-79 0.9796652785516606 1102 80-81 0.9864063861911241 1102 82-83 1.0277569128348674 1102 84-85 0.975796375702398 1102 86-87 1.2196372537746214 1102 88-89 1.0514919473581479 1102 90-91 1.1187185546477814 1102 92-93 1.2433604636332092 1102 94-95 1.4005352606460129 1102 96-97 1.074811171974968 1102 98-99 1.3984259847971714 1102 100-101 1.1241717165880303 1103 1 0.38433985570710405 1103 2 0.2926763997624562 1103 3 0.2520779130488293 1103 4 0.2631362729253013 1103 5 0.354366165251804 1103 6 0.5334440109898466 1103 7 0.4137969670423729 1103 8 0.32599862288914494 1103 9 0.4912231491922867 1103 10-11 0.5730814765973307 1103 12-13 0.6738081936509701 1103 14-15 0.6252718997127076 1103 16-17 0.6305552235952163 1103 18-19 0.7611518713299219 1103 20-21 0.713032933318182 1103 22-23 0.6894148636861388 1103 24-25 0.6365496541919811 1103 26-27 0.7911411921207758 1103 28-29 0.8240804700986573 1103 30-31 0.8488989886910616 1103 32-33 0.7142788243921459 1103 34-35 0.7468509841964632 1103 36-37 0.8767942382147069 1103 38-39 0.8545060088760792 1103 40-41 0.8767384517278316 1103 42-43 0.8628887378386594 1103 44-45 0.8650915708744833 1103 46-47 0.6746476350359671 1103 48-49 0.6086942735061811 1103 50-51 0.7343634620433903 1103 52-53 0.7935148912531602 1103 54-55 0.8806270311316666 1103 56-57 1.0220981926704695 1103 58-59 1.0197221640917924 1103 60-61 1.1713705985343488 1103 62-63 0.9793542265958983 1103 64-65 1.0729233262643767 1103 66-67 1.0655167538648804 1103 68-69 1.2273681455049932 1103 70-71 1.425484345884005 1103 72-73 1.3782399159054215 1103 74-75 1.5742306714484577 1103 76-77 1.4383825698892245 1103 78-79 1.304794553313851 1103 80-81 1.3811156087626237 1103 82-83 1.3211965575044502 1103 84-85 1.2759119685659215 1103 86-87 1.4854165491491642 1103 88-89 1.5136229069119018 1103 90-91 1.7216380416609773 1103 92-93 1.6224115587477215 1103 94-95 1.5086522324403298 1103 96-97 1.714474459170038 1103 98-99 1.8096351168379812 1103 100-101 1.5892184805504712 1104 1 0.17866632504589575 1104 2 0.22756440736536732 1104 3 0.2824531669675956 1104 4 0.22112490303378962 1104 5 0.24617733051437796 1104 6 0.3169548719904114 1104 7 0.1743715368983345 1104 8 0.3091671077348437 1104 9 0.2935830854363175 1104 10-11 0.38827754995883623 1104 12-13 0.5003732966415342 1104 14-15 0.5171408107856976 1104 16-17 0.6217884902383659 1104 18-19 0.600191888889249 1104 20-21 0.5590158551144597 1104 22-23 0.6044380252146695 1104 24-25 0.38841581108667356 1104 26-27 0.4908231400717966 1104 28-29 0.53716126558502 1104 30-31 0.5935651144815495 1104 32-33 0.5488431107056471 1104 34-35 0.5667373963678628 1104 36-37 0.8326681075747189 1104 38-39 0.8182018977957455 1104 40-41 0.834525019381978 1104 42-43 1.0830525405812708 1104 44-45 0.9684477612062068 1104 46-47 0.8358916540830776 1104 48-49 0.5708244178151531 1104 50-51 0.782542908059007 1104 52-53 0.7667423821660933 1104 54-55 0.8668140034470042 1104 56-57 1.1556329372616077 1104 58-59 1.0259197355883778 1104 60-61 0.9753417673823286 1104 62-63 1.103665059556711 1104 64-65 1.0579596917603062 1104 66-67 1.1105128064647047 1104 68-69 1.0861072413147745 1104 70-71 1.1731157142509439 1104 72-73 1.2211450721945063 1104 74-75 1.087437320241694 1104 76-77 1.1097442678114113 1104 78-79 1.351693440495584 1104 80-81 1.1802648587948958 1104 82-83 1.2848572051998453 1104 84-85 1.3556158388567638 1104 86-87 1.4145131212122983 1104 88-89 1.419840742112445 1104 90-91 1.3464713640771926 1104 92-93 1.3540622768451271 1104 94-95 1.671412198716009 1104 96-97 1.7280895422608005 1104 98-99 1.8105185415495058 1104 100-101 1.3835780843012095 1105 1 0.29108422961391156 1105 2 0.11980660470495863 1105 3 0.22531309289480106 1105 4 0.25589289357915845 1105 5 0.25806966616536187 1105 6 0.2790245539029641 1105 7 0.22272337405320286 1105 8 0.17707253839798653 1105 9 0.29658833896886705 1105 10-11 0.2979294423232446 1105 12-13 0.4551158534182136 1105 14-15 0.44835551406318785 1105 16-17 0.48996655393636956 1105 18-19 0.46743020430823634 1105 20-21 0.4134932551478059 1105 22-23 0.40231450681437053 1105 24-25 0.5165260202503106 1105 26-27 0.5606482883036463 1105 28-29 0.3601417323356557 1105 30-31 0.6199389908811952 1105 32-33 0.4519212580806098 1105 34-35 0.6083282218079589 1105 36-37 0.7183773560348925 1105 38-39 0.5683246455629174 1105 40-41 0.6374289475980817 1105 42-43 0.8130083513850863 1105 44-45 0.7853970532589045 1105 46-47 0.6111681690385353 1105 48-49 0.5692752439453699 1105 50-51 0.719769915587058 1105 52-53 0.933068122750278 1105 54-55 0.8789568667516363 1105 56-57 0.5937853155849666 1105 58-59 0.7416802972879175 1105 60-61 0.8018794033214398 1105 62-63 0.9508406747692391 1105 64-65 1.0834337173982362 1105 66-67 1.0504314586618726 1105 68-69 0.9922014253350646 1105 70-71 1.0231125512045303 1105 72-73 1.0091343379651683 1105 74-75 0.9955799362536091 1105 76-77 1.1216051527167785 1105 78-79 1.5358815871229154 1105 80-81 1.6239696416086282 1105 82-83 1.585077935518509 1105 84-85 1.6746126643083592 1105 86-87 1.700947821556781 1105 88-89 1.8642509202207158 1105 90-91 1.9151535023540767 1105 92-93 1.9245888665635 1105 94-95 2.079490116051229 1105 96-97 2.270711952157111 1105 98-99 2.1818506657364622 1105 100-101 1.9885892484173127 1106 1 0.07145818973484452 1106 2 0.11946102135748404 1106 3 0.07744253298024262 1106 4 0.0016890494685384283 1106 5 0.1445010348422997 1106 6 -0.07587268198652453 1106 7 0.24962550790868931 1106 8 0.04451594477897203 1106 9 -0.16759389401411084 1106 10-11 0.036986201432874566 1106 12-13 -0.004430730104481029 1106 14-15 0.18708902758195478 1106 16-17 -0.1364445490933619 1106 18-19 -0.22862124519487992 1106 20-21 0.017928873039714688 1106 22-23 0.11301543954173354 1106 24-25 -0.07387375084418579 1106 26-27 -0.06294321618685217 1106 28-29 0.11478537819129286 1106 30-31 -0.22524010403810735 1106 32-33 0.08932488413299922 1106 34-35 -0.4716915327652842 1106 36-37 -0.3460891488032729 1106 38-39 -0.09000267888490754 1106 40-41 0.26241738759173927 1106 42-43 -0.15090139573412387 1106 44-45 0.04083451518911119 1106 46-47 0.04841115446145494 1106 48-49 0.24977787649779515 1106 50-51 0.05471518895252103 1106 52-53 -0.1321293569249704 1106 54-55 0.10544589986923114 1106 56-57 -0.584583670082715 1106 58-59 -0.35822049525916455 1106 60-61 0.15532346818819676 1106 62-63 0.13706055617226198 1106 64-65 0.3635641351299306 1106 66-67 -0.10792880745816547 1106 68-69 -0.1321053219538122 1106 70-71 0.01105607301174416 1106 72-73 0.30413509588438004 1106 74-75 0.5982781634179943 1106 76-77 0.44647722777441956 1106 78-79 0.43297614901779724 1106 80-81 0.6400127574770913 1106 82-83 0.4397463180493766 1106 84-85 0.6603078083408249 1106 86-87 0.6036549838238088 1106 88-89 0.40213610310870607 1106 90-91 0.5155166951548189 1106 92-93 0.6967037671161549 1106 94-95 0.5559398625307317 1106 96-97 0.643488085072498 1106 98-99 0.5800022294141911 1106 100-101 0.0372115911803661 1107 1 0.12214673062142722 1107 2 -0.06545972916751097 1107 3 -0.011449135680006606 1107 4 0.06039992202070721 1107 5 -0.0029210055666553103 1107 6 0.1800786186312564 1107 7 0.17014742067304667 1107 8 0.13560580796666244 1107 9 0.3485308997288854 1107 10-11 0.3884461509921948 1107 12-13 0.41898359190430057 1107 14-15 0.47010798315255187 1107 16-17 0.5605137635161199 1107 18-19 0.4569842636630952 1107 20-21 0.3694974880643471 1107 22-23 0.4003271786968341 1107 24-25 0.5340524575074923 1107 26-27 0.32408941032878147 1107 28-29 0.443178967492905 1107 30-31 0.5042180584305385 1107 32-33 0.5665683448775596 1107 34-35 0.4856646547126715 1107 36-37 0.4991457627065401 1107 38-39 0.7243667611345757 1107 40-41 0.7013638203284813 1107 42-43 0.7064648951435828 1107 44-45 0.5994931287770413 1107 46-47 0.6782656389607808 1107 48-49 0.6968397116269003 1107 50-51 0.6317604140260755 1107 52-53 0.7554963673593917 1107 54-55 0.7742324832179648 1107 56-57 0.9486678793541046 1107 58-59 0.9065858794717805 1107 60-61 0.8700525660126708 1107 62-63 1.1367582007363204 1107 64-65 1.116092058836486 1107 66-67 1.242839747709219 1107 68-69 1.2213841003341237 1107 70-71 1.254207494273075 1107 72-73 1.2516165787913849 1107 74-75 1.2070447456850495 1107 76-77 1.2570646948471271 1107 78-79 1.4857458409420765 1107 80-81 1.549767830495309 1107 82-83 1.2406146131827889 1107 84-85 1.2234309135386177 1107 86-87 1.3314314030528536 1107 88-89 1.2813436548110708 1107 90-91 1.2024606971031666 1107 92-93 1.3541519801416086 1107 94-95 1.3335312684362286 1107 96-97 1.2876358939951444 1107 98-99 1.1676438304695864 1107 100-101 0.9581805254657958 1108 1 0.3126165566814798 1108 2 0.1644134123736123 1108 3 0.2614407299973962 1108 4 0.13965950498587887 1108 5 0.17272580670388038 1108 6 0.21489148884774778 1108 7 0.25365167427513313 1108 8 0.34253150788914866 1108 9 0.32348711968492694 1108 10-11 0.39103754681801917 1108 12-13 0.36787688980956545 1108 14-15 0.5576570662437206 1108 16-17 0.5527735474388678 1108 18-19 0.48923827404271236 1108 20-21 0.36810785045784655 1108 22-23 0.4170293053383958 1108 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-2.0274856737265132 2316 76-77 -2.2414267847956744 2316 78-79 -2.264188212020784 2316 80-81 -2.4054986274212062 2316 82-83 -2.467888467341009 2316 84-85 -2.4877654149941577 2316 86-87 -2.665449084682738 2316 88-89 -2.959588555113278 2316 90-91 -2.971052479839546 2316 92-93 -2.9094355734639095 2316 94-95 -3.1717585535734454 2316 96-97 -3.334829638169907 2316 98-99 -3.6059515846025114 2316 100-101 -3.008381717811808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8809.0 3 2908.0 4 3082.0 5 3663.0 6 4393.0 7 5400.0 8 6651.0 9 8470.0 10 10613.0 11 13739.0 12 17147.0 13 20571.0 14 23918.0 15 27730.0 16 33009.0 17 38712.0 18 35411.0 19 31160.0 20 32784.0 21 35178.0 22 38549.0 23 42685.0 24 47738.0 25 53303.0 26 59906.0 27 67588.0 28 76865.0 29 87682.0 30 100484.0 31 116622.0 32 135656.0 33 160532.0 34 192960.0 35 232894.0 36 265243.0 37 252408.0 38 110621.0 39 5032.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99926471137432 18.88762283511028 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29.28845971462185 22.359747805354147 44-45 22.063635714807365 26.5287127956925 29.2026842206598 22.204967268840335 46-47 22.34864423645917 26.772190559659652 28.463917542885554 22.415247660995625 48-49 22.296593557253733 26.740463520538576 28.514911797316405 22.448031124891283 50-51 22.56233774074745 26.97122493663162 28.075682290237026 22.3907550323839 52-53 22.514003212175144 26.95372222880389 28.068460286730513 22.463814272290456 54-55 22.290287777247546 27.067659812851424 28.189493121978547 22.452559287922487 56-57 22.313023635179448 27.163929932921167 28.024175686002415 22.498870745896973 58-59 22.405157584799483 26.805559302057762 28.05874699905173 22.73053611409102 60-61 22.295646327532378 26.95398560085162 28.216375270152632 22.533992801463363 62-63 22.14657641455332 26.923590841225277 28.323350659503816 22.606482084717587 64-65 22.14932628021697 26.794884775493134 28.258781262656107 22.79700768163379 66-67 22.188721023261063 26.912535313196095 28.0807132684666 22.818030395076235 68-69 22.309525805297746 26.80390596276787 28.131144044410988 22.755424187523392 70-71 22.295161778335228 26.748055617697027 28.042415113948543 22.914367490019206 72-73 22.347093162363187 26.85238697130131 27.850990188487863 22.94952967784764 74-75 22.171124457263502 27.130464484701488 27.755217149591388 22.943193908443625 76-77 22.353071295337344 26.80597693812754 27.83915247649832 23.001799290036796 78-79 22.43175030627689 26.835000523860554 27.823883269346272 22.90936590051629 80-81 22.266489321642837 27.081941990251835 27.633396217372553 23.018172470732782 82-83 22.43451336558676 27.001591094352996 27.496492185712185 23.067403354348063 84-85 22.53417128943635 26.747153827545418 27.565621614354573 23.15305326866366 86-87 22.406367981488966 26.88664564699399 27.515721182535025 23.19126518898202 88-89 22.551980267370077 26.768076224235564 27.465297198352573 23.214646310041786 90-91 22.59012866095154 26.72977132568346 27.370461192108174 23.309638821256826 92-93 22.52827227043099 26.448372808816035 27.48521640820975 23.538138512543224 94-95 22.518063616808067 26.498597335144332 27.476622944259532 23.506716103788065 96-97 22.721496327981743 26.304580864825937 27.333202578974074 23.640720228218242 98-99 22.657891443971113 26.252126208984684 27.14353096052872 23.946451386515484 100-101 23.380597901142604 25.721528942125715 27.349671435891175 23.548201720840506 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1444.0 1 1000.0 2 730.5 3 919.0 4 788.0 5 721.5 6 1195.5 7 2510.0 8 3992.0 9 4547.5 10 3893.5 11 3057.0 12 2973.5 13 3143.0 14 3339.5 15 3490.5 16 3692.0 17 4031.0 18 4397.5 19 4722.0 20 5119.5 21 5732.0 22 6575.0 23 7718.0 24 9274.5 25 11366.5 26 14242.0 27 17665.0 28 20380.0 29 23128.5 30 26743.5 31 31074.5 32 36161.5 33 40603.0 34 44797.5 35 48613.0 36 52732.5 37 57485.5 38 61013.0 39 63455.5 40 66450.0 41 71724.5 42 76053.0 43 79498.0 44 82844.5 45 85142.0 46 87080.5 47 89119.0 48 90220.5 49 89707.0 50 89168.5 51 86060.0 52 81922.0 53 80339.0 54 79468.5 55 74758.5 56 67679.0 57 61894.0 58 57609.0 59 52840.5 60 45232.0 61 37292.5 62 30617.5 63 24004.5 64 18745.5 65 15166.5 66 11709.0 67 9021.0 68 6961.0 69 5322.0 70 4207.0 71 3195.5 72 2533.0 73 2232.5 74 1808.0 75 1358.0 76 1030.5 77 771.0 78 565.0 79 382.0 80 239.5 81 154.0 82 85.0 83 43.5 84 28.5 85 20.0 86 14.5 87 13.5 88 11.5 89 7.0 90 6.0 91 4.0 92 2.0 93 3.0 94 2.5 95 1.0 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007385536629772177 2 0.10543060997893877 3 0.11364598218508985 4 0.11617698069304547 5 0.15381002408182842 6 0.09236069965097116 7 0.02547595219483212 8 0.02767501647223619 9 0.021949150995221805 10-11 0.0509726502790737 12-13 0.05369036179171459 14-15 0.026782943227628878 16-17 0.030869883441294942 18-19 0.035765913341930425 20-21 0.056428819193764954 22-23 0.03033049031664866 24-25 0.045703194369067716 26-27 0.055370778833881855 28-29 0.05190621530249996 30-31 0.0663868461103117 32-33 0.0484209058817086 34-35 0.061345594983809905 36-37 0.05198919886013786 38-39 0.03138853067653175 40-41 0.0363882900242146 42-43 0.032633284041100094 44-45 0.007468520187410067 46-47 0.013692287010251788 48-49 0.0087962571096163 50-51 0.00827760987437949 52-53 0.009231920787215221 54-55 0.011036813165839321 56-57 0.014086458909031763 58-59 0.019127710035533558 60-61 0.024832829623138472 62-63 0.011368747396390879 64-65 0.0087962571096163 66-67 0.010455928262374094 68-69 0.010870846050563541 70-71 0.030662424547200216 72-73 0.026990402121723604 74-75 0.028318139043929838 76-77 0.0230071913551049 78-79 0.005124234684139684 80-81 0.00516572646295863 82-83 0.0057881031452428015 84-85 0.005186472352368102 86-87 0.012862451433872893 88-89 0.037695281057011365 90-91 0.03477011065027575 92-93 0.028546343827434033 94-95 0.04688571006540764 96-97 0.04225937672709529 98-99 0.02508178029605214 100-101 0.07294254716370499 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2410116.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.031496703349625 #Duplication Level Percentage of deduplicated Percentage of total 1 74.82182940930616 38.93091770248901 2 13.369055168243662 13.912238998267384 3 4.915347920508723 7.672587273652981 4 2.273145132668653 4.73100573906737 5 1.280189828812993 3.330509642877248 6 0.7543436456407693 2.3549777346810252 7 0.4916555927904855 1.7907103448823087 8 0.35126614313933774 1.4621522535002232 9 0.2508212425236753 1.17455441881506 >10 1.320539629362481 12.605128847705647 >50 0.11036898458426277 3.9464445937963846 >100 0.0578371834292978 5.3604419639561485 >500 0.0027200899032043513 0.9689833706166412 >1k 6.40021153694704E-4 0.5095508823658479 >5k 8.0002644211838E-5 0.2944663787154651 >10k+ 1.60005288423676E-4 0.9553298546112418 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12841 0.5327959318140704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10109 0.41944039208071315 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7074 0.2935128433652156 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2508 0.1040613812779136 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2447533645683446E-4 0.0 12-13 0.0 0.0 0.0 1.2447533645683446E-4 0.0 14-15 0.0 0.0 0.0 1.6596711527577926E-4 0.0 16-17 0.0 0.0 0.0 1.8671300468525167E-4 0.0 18-19 0.0 0.0 0.0 2.0745889409472408E-4 0.0 20-21 0.0 0.0 0.0 3.1118834114208615E-4 0.0 22-23 0.0 0.0 0.0 5.60139014055755E-4 0.0 24-25 0.0 0.0 0.0 0.0012447533645683444 0.0 26-27 0.0 0.0 0.0 0.0021160807197661852 0.0 28-29 0.0 0.0 0.0 0.006182275044022778 0.0 30-31 0.0 0.0 0.0 0.01636850674407373 0.0 32-33 0.0 0.0 0.0 0.03506055310200837 0.0 34-35 0.0 0.0 0.0 0.063150487362434 0.0 36-37 0.0 0.0 0.0 0.11406089997327928 0.0 38-39 0.0 0.0 0.0 0.18860088062151364 0.0 40-41 0.0 0.0 0.0 0.27141847114412754 0.0 42-43 0.0 0.0 0.0 0.35496216779607287 0.0 44-45 0.0 0.0 0.0 0.459749655203318 0.0 46-47 0.0 0.0 2.0745889409472407E-5 0.5857186956976345 0.0 48-49 0.0 0.0 4.1491778818944814E-5 0.7194052070522747 0.0 50-51 0.0 0.0 4.1491778818944814E-5 0.8630912371022805 4.1491778818944814E-5 52-53 0.0 0.0 4.1491778818944814E-5 1.0049308829948433 4.1491778818944814E-5 54-55 0.0 0.0 4.1491778818944814E-5 1.1579940550579306 4.1491778818944814E-5 56-57 0.0 0.0 4.1491778818944814E-5 1.3416366681105805 4.1491778818944814E-5 58-59 0.0 0.0 4.1491778818944814E-5 1.5416685337967135 4.1491778818944814E-5 60-61 0.0 0.0 4.1491778818944814E-5 1.7601434951678674 4.1491778818944814E-5 62-63 0.0 0.0 4.1491778818944814E-5 1.977332211395634 4.1491778818944814E-5 64-65 0.0 0.0 4.1491778818944814E-5 2.2002260472110056 4.1491778818944814E-5 66-67 0.0 0.0 4.1491778818944814E-5 2.456313306081533 4.1491778818944814E-5 68-69 0.0 0.0 4.1491778818944814E-5 2.748747363197456 4.1491778818944814E-5 70-71 0.0 0.0 4.1491778818944814E-5 3.0614916460452526 4.1491778818944814E-5 72-73 0.0 0.0 4.1491778818944814E-5 3.376289771944587 4.1491778818944814E-5 74-75 0.0 0.0 4.1491778818944814E-5 3.6871254329667122 4.1491778818944814E-5 76-77 0.0 0.0 4.1491778818944814E-5 4.031403467716906 4.1491778818944814E-5 78-79 0.0 0.0 4.1491778818944814E-5 4.410534596675015 4.1491778818944814E-5 80-81 0.0 0.0 4.1491778818944814E-5 4.81889668381107 4.1491778818944814E-5 82-83 0.0 0.0 4.1491778818944814E-5 5.242921917451277 4.1491778818944814E-5 84-85 0.0 0.0 4.1491778818944814E-5 5.658814762442969 4.1491778818944814E-5 86-87 0.0 0.0 4.1491778818944814E-5 6.091802220308068 4.1491778818944814E-5 88-89 0.0 0.0 6.223766822841721E-5 6.550120409142132 6.223766822841721E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6290 0.0 57.15826 1 GTATCAA 11505 0.0 43.56742 1 TATCAAC 15150 0.0 32.89565 2 ATCAACG 15520 0.0 32.14205 3 TCAACGC 15615 0.0 31.85514 4 CAACGCA 15970 0.0 31.087471 5 TGGTATC 2720 0.0 30.245935 2 AACGCAG 16495 0.0 30.104958 6 GTGGTAT 3375 0.0 26.067886 1 ACGCAGA 18930 0.0 26.059488 7 CGCAGAG 19070 0.0 25.893099 8 GCAGAGT 20115 0.0 23.744125 9 TATACCG 265 3.092282E-11 23.328527 5 GTACATG 15090 0.0 23.19511 1 TACATGG 14975 0.0 23.18173 2 GAGTACT 10725 0.0 22.419569 12-13 ACATGGG 15375 0.0 21.496092 3 CAGAGTA 19410 0.0 21.117338 10-11 GTACTTT 11365 0.0 20.941032 14-15 AGTACTT 11165 0.0 20.812498 12-13 >>END_MODULE