FastQCFastQC Report
Thu 26 May 2016
SRR938659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938659_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410116
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123480.5123404848563305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108090.4484846372539745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74070.30732960571192425No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25320.10505718396956827No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61800.058.912571
GTATCAA114650.043.5294271
ATCAACG152250.032.576433
TATCAAC152750.032.547542
TCAACGC154850.032.0294574
CAACGCA158600.031.2421885
AACGCAG164000.030.1990666
TGGTATC25250.029.7415332
ACGCAGA189350.026.0306537
CGCAGAG190100.025.803068
GTGGTAT32100.025.3197141
GCAGAGT204050.023.7830379
TAGATCG1201.7097939E-423.7536475
TACATGG154800.023.4886572
GTACATG159800.022.9321781
GAGTACT110100.022.21124812-13
ACATGGG159250.021.866783
ACCGAGT10450.021.3567228
GTACTTT114150.021.17360914-15
CAGAGTA198500.020.93150910-11
Produced by FastQC (version 0.11.5)