FastQCFastQC Report
Thu 26 May 2016
SRR938658_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938658_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408897
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130570.5420323077325431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101970.4233057702342608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72880.30254510674387486No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25050.10398950224936973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61000.056.809141
GTATCAA115250.044.8957561
TATCAAC152150.033.735112
ATCAACG154750.033.1690063
TCAACGC157200.032.6822934
CAACGCA160200.032.185595
AACGCAG165750.031.1307336
TGGTATC25350.029.8091432
ACGCAGA189950.027.0356187
CGCAGAG191450.026.6997288
GTGGTAT31200.025.1736371
TACATGG153400.025.064182
GTACATG156200.024.8976121
GCAGAGT202300.024.5152579
ACATGGG157500.023.2353673
GAGTACT106800.022.66069612-13
CAGAGTA196050.021.28551910-11
AGTACTT111800.021.05242512-13
AGAGTAC193700.020.84484510-11
GTACTTT116700.020.59757414-15
Produced by FastQC (version 0.11.5)