##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938658_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2408897 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.063132628750836 31.0 31.0 34.0 28.0 34.0 2 31.14428470789743 31.0 31.0 34.0 27.0 34.0 3 31.19284137096771 31.0 31.0 34.0 27.0 34.0 4 34.84312737323348 37.0 35.0 37.0 32.0 37.0 5 34.87450106833128 37.0 35.0 37.0 32.0 37.0 6 34.914303932463696 37.0 35.0 37.0 32.0 37.0 7 34.83931608532868 37.0 35.0 37.0 32.0 37.0 8 34.80990552937714 37.0 35.0 37.0 32.0 37.0 9 36.29589683577172 39.0 35.0 39.0 32.0 39.0 10-11 36.1416760450945 39.0 35.0 39.0 31.0 39.0 12-13 35.99232075925205 38.0 35.0 39.0 30.0 39.0 14-15 36.93589119833683 39.0 36.0 41.0 31.0 41.0 16-17 36.79846274041605 39.0 36.0 41.0 30.5 41.0 18-19 36.83468076883321 39.0 36.0 41.0 30.5 41.0 20-21 36.94323231752956 39.0 36.0 41.0 30.5 41.0 22-23 36.856520017252706 39.0 36.0 41.0 30.0 41.0 24-25 36.73939691070228 39.0 36.0 41.0 30.0 41.0 26-27 36.553683283262004 39.0 36.0 41.0 30.0 41.0 28-29 36.35972106735987 39.0 35.0 41.0 29.5 41.0 30-31 36.107593641405174 39.0 35.0 40.0 29.0 41.0 32-33 35.80613969796135 38.0 35.0 40.0 27.5 41.0 34-35 35.54034875712826 38.0 34.5 40.0 27.0 41.0 36-37 35.25380952361184 38.0 34.0 40.0 26.0 41.0 38-39 34.97885629813147 38.0 34.0 40.0 25.0 41.0 40-41 34.8766867159534 38.0 34.0 40.0 25.0 41.0 42-43 34.65519364256753 38.0 33.5 40.0 24.0 41.0 44-45 34.304869199471796 38.0 33.0 40.0 23.5 41.0 46-47 33.860758471615846 38.0 33.0 40.0 21.5 41.0 48-49 33.44537665994021 38.0 32.5 40.0 18.5 41.0 50-51 32.68751154574065 37.0 31.5 39.5 16.0 40.5 52-53 32.74463499269583 37.0 31.5 39.5 15.5 40.0 54-55 32.86804956791428 37.0 32.0 40.0 14.5 41.0 56-57 32.57224945690912 37.0 31.0 40.0 11.0 41.0 58-59 32.24928982019571 36.5 31.0 40.0 8.5 41.0 60-61 31.80922741819181 36.0 31.0 39.0 7.5 41.0 62-63 31.295177419374923 35.5 30.0 39.0 4.0 40.0 64-65 30.80337245635658 35.0 29.0 39.0 2.0 40.0 66-67 30.269646024715875 35.0 28.5 38.0 2.0 40.0 68-69 29.7105401351739 34.0 28.0 37.5 2.0 40.0 70-71 29.103780692989364 34.0 27.0 37.0 2.0 39.0 72-73 28.552263338781195 34.0 26.0 36.0 2.0 39.0 74-75 27.944822464389304 33.5 26.0 36.0 2.0 39.0 76-77 27.21455774157218 33.0 24.5 35.0 2.0 38.0 78-79 26.44505804938941 32.0 22.5 35.0 2.0 37.0 80-81 25.795282654260433 32.0 20.5 35.0 2.0 36.5 82-83 25.148638982903794 31.0 19.0 35.0 2.0 36.0 84-85 24.489049344990676 31.0 15.0 34.0 2.0 35.5 86-87 23.821915382849497 31.0 7.5 34.0 2.0 35.0 88-89 23.038272703233055 30.0 2.0 34.0 2.0 35.0 90-91 22.205266352193554 29.0 2.0 34.0 2.0 35.0 92-93 21.35437276894778 29.0 2.0 34.0 2.0 35.0 94-95 20.395726965494994 28.0 2.0 34.0 2.0 35.0 96-97 19.158415449062375 26.0 2.0 33.0 2.0 35.0 98-99 17.710224222953492 24.0 2.0 33.0 2.0 34.0 100-101 14.33963614882662 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6328136349312992 1101 2 0.5268155000540489 1101 3 0.5983514588168788 1101 4 0.5014343944078092 1101 5 0.5197771768357313 1101 6 0.6650303100842905 1101 7 0.5105293945265927 1101 8 0.4649031160937085 1101 9 0.5452825562982397 1101 10-11 0.629523278581388 1101 12-13 0.6141230129797819 1101 14-15 0.6547325488200286 1101 16-17 0.7575918260210699 1101 18-19 0.6536906965007603 1101 20-21 0.837495021886383 1101 22-23 0.8943225377772066 1101 24-25 0.9232154164170296 1101 26-27 0.8513044405252472 1101 28-29 0.8608763724308872 1101 30-31 1.0197193322809923 1101 32-33 1.0243759722861583 1101 34-35 1.0368814572595468 1101 36-37 0.9727154572685848 1101 38-39 1.077236097087031 1101 40-41 0.8366133182857638 1101 42-43 0.9301368582171392 1101 44-45 1.0185734712608152 1101 46-47 1.0035007813223586 1101 48-49 1.0946912560122257 1101 50-51 1.0335976931741868 1101 52-53 1.0928235548710816 1101 54-55 1.135277525720923 1101 56-57 1.0818109121935535 1101 58-59 1.2258828454322028 1101 60-61 1.2484669894851557 1101 62-63 1.1095712180931372 1101 64-65 1.2816671638580992 1101 66-67 1.163632186105712 1101 68-69 1.1421455092027628 1101 70-71 1.2629525761953495 1101 72-73 1.2069517957740956 1101 74-75 1.276773650267188 1101 76-77 1.3425850097731775 1101 78-79 1.165877767368535 1101 80-81 1.178639612390441 1101 82-83 1.1777021446982339 1101 84-85 1.445237685498558 1101 86-87 1.4324051979222538 1101 88-89 1.6420968428835714 1101 90-91 1.6071712005157828 1101 92-93 1.4528444247039154 1101 94-95 1.1288399729192555 1101 96-97 1.4256303292676584 1101 98-99 1.167814481082683 1101 100-101 0.9697250088632057 1102 1 0.5291223213441718 1102 2 0.6003820757805727 1102 3 0.576897216975663 1102 4 0.49830687249421146 1102 5 0.6683819390463341 1102 6 0.60571629850687 1102 7 0.4413357646185503 1102 8 0.48982834877296 1102 9 0.6816983603366538 1102 10-11 0.6688057915452461 1102 12-13 0.7124975393137944 1102 14-15 0.7959946156093736 1102 16-17 0.7961538919726863 1102 18-19 0.8769413087411024 1102 20-21 0.783347006326963 1102 22-23 0.8240845389444402 1102 24-25 0.6823864555641066 1102 26-27 0.6761576285032689 1102 28-29 0.5614091681283071 1102 30-31 0.5172354655469391 1102 32-33 0.5578613999887452 1102 34-35 0.6424897742339013 1102 36-37 0.6570898551508009 1102 38-39 0.7068397674573887 1102 40-41 0.6937735283882205 1102 42-43 0.8163760410403427 1102 44-45 0.7799561151896555 1102 46-47 0.6880846114755812 1102 48-49 0.4807601005883413 1102 50-51 0.6181329350405349 1102 52-53 0.6635195122697723 1102 54-55 0.5857942649442336 1102 56-57 0.6674184472874174 1102 58-59 0.7784615151742713 1102 60-61 0.6180200051702336 1102 62-63 0.6228394505685344 1102 64-65 0.6715756000692181 1102 66-67 0.6441494000421741 1102 68-69 0.5996503052017665 1102 70-71 0.5859934769216615 1102 72-73 0.639143211284928 1102 74-75 0.7476555835225227 1102 76-77 0.7552657149176731 1102 78-79 0.6528552687577722 1102 80-81 0.9509420549292074 1102 82-83 0.9124575696921617 1102 84-85 0.7663559979770902 1102 86-87 0.9780753951516061 1102 88-89 1.1312440626003628 1102 90-91 1.1320545467408358 1102 92-93 1.319695764093101 1102 94-95 1.0981623338632112 1102 96-97 1.0698481835083804 1102 98-99 0.777783177939245 1102 100-101 0.8035851807093639 1103 1 0.5729176744641507 1103 2 0.6276763485349548 1103 3 0.5696537972653992 1103 4 0.5729358074577675 1103 5 0.5741782443529431 1103 6 0.5929376400436581 1103 7 0.49703083469843534 1103 8 0.4154165332878961 1103 9 0.5087927785349251 1103 10-11 0.48774907365930886 1103 12-13 0.4796120476035739 1103 14-15 0.6528448659507475 1103 16-17 0.7305825015186187 1103 18-19 0.888865876725653 1103 20-21 0.7913268011359733 1103 22-23 0.8182510878957245 1103 24-25 0.8710776698113349 1103 26-27 0.8782147701645755 1103 28-29 0.8135934423999132 1103 30-31 0.9574936022955853 1103 32-33 0.9821377119920456 1103 34-35 1.1211532076514672 1103 36-37 0.9729568420033061 1103 38-39 0.9934335552244065 1103 40-41 1.0394198904598042 1103 42-43 0.816280560787753 1103 44-45 1.06851705774978 1103 46-47 1.0584917497362127 1103 48-49 0.9481856390708145 1103 50-51 1.1874411348323122 1103 52-53 1.247444038399962 1103 54-55 1.1645759369211746 1103 56-57 1.1701455202552609 1103 58-59 1.108705473759379 1103 60-61 1.1281448357908808 1103 62-63 1.1006671047887693 1103 64-65 0.9269970472882569 1103 66-67 1.1411712009305326 1103 68-69 1.1501044249784762 1103 70-71 1.0316992198626629 1103 72-73 1.186488337083432 1103 74-75 0.9431311274983685 1103 76-77 1.0367166485314279 1103 78-79 1.0046476816091463 1103 80-81 0.8898268455789413 1103 82-83 0.920395510143873 1103 84-85 0.9626607814069494 1103 86-87 0.8850069998999821 1103 88-89 1.046622399831385 1103 90-91 1.2039407133525657 1103 92-93 1.2537725354479754 1103 94-95 1.1739043331412233 1103 96-97 1.1685799964821904 1103 98-99 1.4551423249474453 1103 100-101 0.9006415114424957 1104 1 0.44456956868820185 1104 2 0.46305654877218316 1104 3 0.45030469979701593 1104 4 0.4281698731794137 1104 5 0.5308834920873196 1104 6 0.4493395465637846 1104 7 0.40691664709127906 1104 8 0.35215639303261526 1104 9 0.5129471348327428 1104 10-11 0.512484193411602 1104 12-13 0.4576993050993181 1104 14-15 0.45682204444280927 1104 16-17 0.6362816446826329 1104 18-19 0.5902809543395975 1104 20-21 0.6298865365931476 1104 22-23 0.7038747503405531 1104 24-25 0.791962746727549 1104 26-27 0.6468644785475206 1104 28-29 0.7583792219651002 1104 30-31 0.7146176027393878 1104 32-33 0.5256723806215078 1104 34-35 0.499963988846595 1104 36-37 0.5942372804983549 1104 38-39 0.6543076285867713 1104 40-41 0.6311766044005722 1104 42-43 0.532836391241517 1104 44-45 0.45838801191346334 1104 46-47 0.5883303109014477 1104 48-49 0.4497826985369002 1104 50-51 0.1414624076072073 1104 52-53 0.21961231041879614 1104 54-55 0.3642060825409672 1104 56-57 0.38511887495516106 1104 58-59 0.19260185276662156 1104 60-61 0.3133136579874112 1104 62-63 0.38317534315639534 1104 64-65 0.44520895834934393 1104 66-67 0.29138543852265286 1104 68-69 0.30483503807773715 1104 70-71 0.3617637999718539 1104 72-73 0.4610064168832153 1104 74-75 0.32078865160724845 1104 76-77 0.49980298525161615 1104 78-79 0.5160890327815082 1104 80-81 0.5923883173557414 1104 82-83 0.573747819460408 1104 84-85 0.4102943882277792 1104 86-87 0.45179067941061035 1104 88-89 0.5384286412026889 1104 90-91 0.5952780454826012 1104 92-93 0.6056483439861395 1104 94-95 0.4871965003723666 1104 96-97 0.36176728680558057 1104 98-99 0.11770582128664486 1104 100-101 -0.03764908303536707 1105 1 0.4815450915248505 1105 2 0.48801540977356694 1105 3 0.488204137479908 1105 4 0.4884540098950154 1105 5 0.4257957048534635 1105 6 0.4515708234519593 1105 7 0.5044405913585663 1105 8 0.4635078722338051 1105 9 0.5795506688205521 1105 10-11 0.6382243168069692 1105 12-13 0.5875556719452462 1105 14-15 0.6848264713019176 1105 16-17 0.562889905508932 1105 18-19 0.6927523950049803 1105 20-21 0.7405589158478492 1105 22-23 0.7630118805763075 1105 24-25 0.7068256612002557 1105 26-27 0.7598364593199989 1105 28-29 0.7628399729807711 1105 30-31 0.8107520692159724 1105 32-33 0.834048295161999 1105 34-35 0.9220508357604871 1105 36-37 0.9697877534706478 1105 38-39 0.7815995683716821 1105 40-41 0.8359374970411224 1105 42-43 0.8691988132000077 1105 44-45 0.7294438059141655 1105 46-47 0.6770406483638212 1105 48-49 0.6306136558316595 1105 50-51 0.4938960804364356 1105 52-53 0.5004809814668931 1105 54-55 0.5856322356948667 1105 56-57 0.7405814561334765 1105 58-59 0.8754663241034351 1105 60-61 0.8463674552503768 1105 62-63 0.8837250950244169 1105 64-65 0.8575245719874438 1105 66-67 0.6802237900206052 1105 68-69 0.6106794259395336 1105 70-71 0.6942958061848863 1105 72-73 0.7276816827183055 1105 74-75 0.7286326075275866 1105 76-77 0.814808371900515 1105 78-79 1.2445205336446712 1105 80-81 1.0582882879346904 1105 82-83 1.1302112753382012 1105 84-85 1.1261487489616364 1105 86-87 1.124997581532245 1105 88-89 0.9563934758318453 1105 90-91 0.9942282618859934 1105 92-93 1.117624263430141 1105 94-95 1.2087330726176404 1105 96-97 1.0324293218195173 1105 98-99 0.8417711259315688 1105 100-101 0.6989130487507662 1106 1 0.2663920306580927 1106 2 0.31758287084225856 1106 3 0.12585830265663134 1106 4 0.30661780535577066 1106 5 0.367129325657956 1106 6 -0.07607180782069634 1106 7 0.27692784797216063 1106 8 0.2764623045483674 1106 9 0.19346655195300855 1106 10-11 0.012041039916141472 1106 12-13 0.01758089578712685 1106 14-15 0.2793889717401399 1106 16-17 0.1646323488736101 1106 18-19 -0.12809662654133547 1106 20-21 0.08541090325520884 1106 22-23 0.19969679465047108 1106 24-25 0.28741513484756354 1106 26-27 0.08071825959064682 1106 28-29 0.08186635722454838 1106 30-31 0.20233731493370755 1106 32-33 0.35228518542513854 1106 34-35 0.31482970031949975 1106 36-37 0.13961739174778387 1106 38-39 0.045518869606404166 1106 40-41 0.16132514489596161 1106 42-43 -0.1889624756524242 1106 44-45 0.24176080597726468 1106 46-47 -0.0620308022437186 1106 48-49 0.31914110231645054 1106 50-51 -0.0946312784047052 1106 52-53 0.1287148567885481 1106 54-55 0.09119261165105996 1106 56-57 -0.18991292415581995 1106 58-59 -0.5574473714435584 1106 60-61 0.18084629106227723 1106 62-63 0.2862039203190747 1106 64-65 0.5291502038981513 1106 66-67 0.029800120626465088 1106 68-69 -0.2661234915422206 1106 70-71 -0.17396755245993134 1106 72-73 0.5004771820416956 1106 74-75 0.6313204629225808 1106 76-77 0.5379697834540522 1106 78-79 0.5549474107963199 1106 80-81 0.3891061609285238 1106 82-83 0.41661274985493435 1106 84-85 0.525499923035941 1106 86-87 0.5861263966602372 1106 88-89 0.4524801869513695 1106 90-91 0.5368539253484101 1106 92-93 0.5684475878588806 1106 94-95 0.3668979548596347 1106 96-97 0.3463700081043406 1106 98-99 0.18340834433252695 1106 100-101 -0.19368115064204083 1107 1 0.5294839427402174 1107 2 0.5730382568220378 1107 3 0.5182626826237211 1107 4 0.47680432194410827 1107 5 0.5639807318460086 1107 6 0.45394796624032807 1107 7 0.431632590812967 1107 8 0.3633365695174362 1107 9 0.47104306851619526 1107 10-11 0.6098491284207839 1107 12-13 0.4610058738043037 1107 14-15 0.6128301026253098 1107 16-17 0.5171668067885591 1107 18-19 0.5780848840829265 1107 20-21 0.6068440071624934 1107 22-23 0.519121226135745 1107 24-25 0.5463217189644354 1107 26-27 0.5451773396902624 1107 28-29 0.5896410251351583 1107 30-31 0.6721527430601455 1107 32-33 0.6597207017846358 1107 34-35 0.6241552782644177 1107 36-37 0.7102765913802997 1107 38-39 0.7836683896725916 1107 40-41 0.7423235212267727 1107 42-43 0.8314367441145905 1107 44-45 0.7370985199482831 1107 46-47 0.7964334430853981 1107 48-49 0.904330590462493 1107 50-51 0.9450307603437409 1107 52-53 0.7951163045951688 1107 54-55 0.967365077675133 1107 56-57 0.9071703389749857 1107 58-59 0.8520419146030278 1107 60-61 0.9146663898571425 1107 62-63 1.0262733432836413 1107 64-65 0.9190338311600321 1107 66-67 0.7870033190116814 1107 68-69 0.9292703006264453 1107 70-71 0.8441835099662534 1107 72-73 0.8448472746755016 1107 74-75 1.11149384430378 1107 76-77 1.224149986425278 1107 78-79 1.1118522798759436 1107 80-81 1.169868321473114 1107 82-83 1.2336922073800984 1107 84-85 1.2703580384899702 1107 86-87 1.2590381124770609 1107 88-89 1.5225280587879588 1107 90-91 1.5750427588296922 1107 92-93 1.5521198631835524 1107 94-95 1.4953993425645074 1107 96-97 1.416784693383267 1107 98-99 1.280782979384167 1107 100-101 0.9796722333712307 1108 1 0.2850510523706511 1108 2 0.29875346740054454 1108 3 0.30626440380411424 1108 4 0.3567752232577064 1108 5 0.2361324443843955 1108 6 0.2870798096945393 1108 7 0.2807473618656786 1108 8 0.18357463055529166 1108 9 0.1715999089916238 1108 10-11 0.20282709231963736 1108 12-13 0.3286194094658157 1108 14-15 0.4404834150733947 1108 16-17 0.3768650162366143 1108 18-19 0.4198465696717406 1108 20-21 0.3630190400939597 1108 22-23 0.5127176627083472 1108 24-25 0.34994004391557354 1108 26-27 0.4414237881073575 1108 28-29 0.32450475533998 1108 30-31 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62-63 -0.6888559613501286 2316 64-65 -0.8252692558344172 2316 66-67 -0.6683146272851843 2316 68-69 -0.5510065085617093 2316 70-71 -0.9366123274665945 2316 72-73 -0.7037341744494796 2316 74-75 -0.5265094407892335 2316 76-77 -0.4110596328864453 2316 78-79 -0.4827007145030855 2316 80-81 -0.7263872101129287 2316 82-83 -0.5395053468090971 2316 84-85 -0.47300846501883953 2316 86-87 -0.4720651846317274 2316 88-89 -0.6080311062308219 2316 90-91 -0.6159338262893854 2316 92-93 -0.5640697373906214 2316 94-95 -0.8567214549651219 2316 96-97 -0.8475967693145314 2316 98-99 -0.8538346651083693 2316 100-101 -0.8175263008814895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7791.0 3 2629.0 4 2893.0 5 3127.0 6 3893.0 7 4869.0 8 6299.0 9 7891.0 10 9887.0 11 12544.0 12 15773.0 13 18829.0 14 21977.0 15 26470.0 16 30924.0 17 36721.0 18 33410.0 19 28600.0 20 29784.0 21 32073.0 22 35148.0 23 39226.0 24 43719.0 25 48983.0 26 55088.0 27 62720.0 28 71602.0 29 81896.0 30 95247.0 31 110865.0 32 131475.0 33 157620.0 34 192291.0 35 237163.0 36 281110.0 37 282062.0 38 138480.0 39 7818.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.05274819966763 18.876707383816388 14.924917531552603 22.14562688496338 2 15.567344236640446 20.28743597293929 40.56021108319687 23.585008707223405 3 19.253871618706036 25.35260812270567 29.526385233588737 25.867135024999556 4 12.840099747640462 16.6740458433776 36.7501690426785 33.73568536630344 5 13.68355075506753 37.960891917161675 33.360290388542964 14.995266939227832 6 31.612232978897513 35.85935663210925 17.634172054927443 14.894238334065799 7 28.637135464227566 33.384078797490304 20.06858527708809 17.910200461194048 8 26.800677042109992 34.58279937644355 19.41903275664605 19.197490824800404 9 27.434479831594615 16.58626104080608 19.939709277287907 36.039549850311396 10-11 25.16124182733747 26.916717384050305 26.54602885481705 21.376011933795176 12-13 26.430354598913926 24.465429320766493 27.174323581034376 21.929892499285202 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26.423483536266225 29.350288802285917 22.238180864287113 40-41 22.358786784612164 26.209856233162704 29.449198287287864 21.98215869493727 42-43 22.05941093135228 26.318462123590148 29.28790838112416 22.334218563933415 44-45 22.040900880480272 26.54322755862984 29.211798078627567 22.204073482262324 46-47 22.335241185697168 26.750488449508065 28.53375470493332 22.380515659861448 48-49 22.258247324238372 26.709954912690044 28.58780400720733 22.443993755864255 50-51 22.522959937211112 27.05369043509452 28.127316466327468 22.2960331613669 52-53 22.47879391377074 26.995659662217967 28.119219482343777 22.406326941667512 54-55 22.29463487623145 27.072250875905958 28.246809726116172 22.38630452174642 56-57 22.28161794957701 27.20555419244299 28.031206005887814 22.481621852092186 58-59 22.37612166350064 26.80060374231388 28.09753705768413 22.725737536501352 60-61 22.2112086601334 26.981252749209983 28.29198888255292 22.5155497081037 62-63 22.133153161007222 26.989025678707907 28.350343842693782 22.52747731759109 64-65 22.15168971156906 26.86706055179483 28.22405660720057 22.757193129435542 66-67 22.150005625200567 26.950335913729766 28.11682815074541 22.782830310324258 68-69 22.311297509568444 26.805632418164326 28.126594481087093 22.756475591180134 70-71 22.266216765256473 26.794386017652116 28.09256182424738 22.846835392844024 72-73 22.343687978834538 26.8253425851958 27.86939494746003 22.96157448850963 74-75 22.130372267389454 27.18005760534649 27.777846978376612 22.911723148887443 76-77 22.296045038237665 26.832771462497195 27.911584226652607 22.959599272612532 78-79 22.436202808427733 26.870968793194745 27.85372545625171 22.839102942125812 80-81 22.242121823337623 27.09850323309705 27.705703996477855 22.953670947087474 82-83 22.37924552427544 26.99871552807821 27.56574719400591 23.056291753640444 84-85 22.517266989567776 26.761996805861205 27.651988324413907 23.068747880157108 86-87 22.370683400931743 26.890716007589955 27.573882373992227 23.164718217486076 88-89 22.511422815567542 26.786930725219804 27.560009359576966 23.141637099635687 90-91 22.534197069887455 26.754931190588955 27.483337759432942 23.22753398009065 92-93 22.486912924103784 26.491521816983354 27.557068785650152 23.46449647326271 94-95 22.470560315822716 26.553923460662205 27.5682759620154 23.40724026149968 96-97 22.700840957988643 26.355733825231397 27.41171726769008 23.531707949089878 98-99 22.5882267456948 26.29355284054932 27.27450512264239 23.843715291113487 100-101 23.266152386030925 25.83286592828098 27.356318924927507 23.544662760760584 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1436.0 1 968.0 2 741.5 3 963.5 4 772.5 5 733.0 6 1239.0 7 2631.5 8 4187.5 9 4678.5 10 4030.0 11 3224.0 12 3087.5 13 3277.0 14 3445.5 15 3628.5 16 3870.0 17 4096.0 18 4387.0 19 4748.5 20 5162.0 21 5798.5 22 6705.5 23 7857.0 24 9390.0 25 11490.5 26 14355.5 27 17562.5 28 20272.5 29 22976.5 30 26548.0 31 30982.0 32 36060.0 33 40515.5 34 44627.0 35 48344.5 36 52695.5 37 57851.0 38 61502.0 39 64033.0 40 66883.0 41 71605.0 42 75844.5 43 79730.5 44 82944.0 45 84937.0 46 87098.0 47 88998.5 48 90316.0 49 89941.0 50 88715.5 51 85554.0 52 81643.0 53 80081.5 54 79511.5 55 74819.0 56 67622.5 57 62021.5 58 57749.0 59 52585.5 60 44766.5 61 37267.0 62 30615.5 63 23703.5 64 18435.5 65 14866.5 66 11498.0 67 8858.0 68 6824.5 69 5193.5 70 4059.0 71 3164.0 72 2571.5 73 2177.5 74 1679.5 75 1277.0 76 963.5 77 716.5 78 545.0 79 364.5 80 219.5 81 142.5 82 83.5 83 35.5 84 29.0 85 24.5 86 15.5 87 12.5 88 11.0 89 6.0 90 3.5 91 2.5 92 1.0 93 2.0 94 2.5 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18020695779022516 2 0.04674338504303007 3 0.04375446521789848 4 0.0506870987011898 5 0.05662342557610392 6 0.06239370134962184 7 0.03321022027923984 8 0.03217240089551359 9 0.037278472263446716 10-11 0.03925032909252658 12-13 0.035970819839951644 14-15 0.03188181146807024 16-17 0.030076005740386574 18-19 0.03775586917996079 20-21 0.04832087050629396 22-23 0.04493757931534641 24-25 0.06550715950080058 26-27 0.0503549964983974 28-29 0.042343030856030785 30-31 0.05195323834933581 32-33 0.047718935263732734 34-35 0.04699246169512437 36-37 0.044231862134412554 38-39 0.024430268292915804 40-41 0.022956564768024535 42-43 0.02013369604428915 44-45 0.00406825198420688 46-47 0.010585757714007696 48-49 0.009838527757724801 50-51 0.007908183703993986 52-53 0.008862977537022131 54-55 0.010295168286564349 56-57 0.00805347841771566 58-59 0.010523488550984122 60-61 0.010398950224936974 62-63 0.003819175332112581 64-65 0.006538262117475343 66-67 0.0039022008828106802 68-69 0.008219529519111859 70-71 0.021482861243133266 72-73 0.018473185030327157 74-75 0.01826562115358191 76-77 0.011499038771686792 78-79 0.0046286744514190525 80-81 0.005749519385843396 82-83 0.005189096918631224 84-85 0.004566405288395478 86-87 0.0077421326025977864 88-89 0.027751290320839787 90-91 0.03455938547808395 92-93 0.021150759040340868 94-95 0.029494826885499877 96-97 0.03362534803273033 98-99 0.019594029964751503 100-101 0.11966057494363604 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2408897.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.547285883863516 #Duplication Level Percentage of deduplicated Percentage of total 1 74.1182556142258 38.20594911359769 2 13.814997758444642 14.24251277878959 3 5.05356306564606 7.814923802309735 4 2.3342992637859554 4.81307165955467 5 1.2821800507474426 3.3046450815232524 6 0.7551376910543925 2.3355179065476825 7 0.5098300456627286 1.8396248581171903 8 0.3558884391612418 1.4676066492965196 9 0.25576785926455053 1.18657250652722 >10 1.347072702591307 12.739531059624762 >50 0.11178398228917337 3.9684828303499016 >100 0.057502708889330775 5.294485459665409 >500 0.002750170001427902 0.9636602733104859 >1k 7.27986176848345E-4 0.5499601507858416 >5k 8.088735298314944E-5 0.30387487330759766 >10k+ 1.6177470596629887E-4 0.9695809966924913 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13057 0.5420323077325431 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10197 0.4233057702342608 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7288 0.30254510674387486 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2505 0.10398950224936973 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.1512775349049794E-5 0.0 8 0.0 0.0 0.0 4.1512775349049794E-5 0.0 9 0.0 0.0 0.0 4.1512775349049794E-5 0.0 10-11 0.0 0.0 0.0 8.302555069809959E-5 0.0 12-13 0.0 0.0 0.0 8.302555069809959E-5 0.0 14-15 0.0 0.0 0.0 1.0378193837262448E-4 0.0 16-17 0.0 0.0 0.0 2.0756387674524896E-4 0.0 18-19 0.0 0.0 0.0 2.2832026441977385E-4 0.0 20-21 0.0 0.0 0.0 3.94371365815973E-4 0.0 22-23 0.0 0.0 0.0 7.057171809338465E-4 0.0 24-25 0.0 0.0 0.0 0.0015152163002403174 0.0 26-27 0.0 0.0 0.0 0.0025737920716410873 0.0 28-29 0.0 0.0 0.0 0.005894814099565071 0.0 30-31 0.0 0.0 0.0 0.017393852871251864 0.0 32-33 0.0 0.0 0.0 0.03821250970880034 0.0 34-35 0.0 0.0 0.0 0.06992827007547438 0.0 36-37 0.0 0.0 0.0 0.12173621371108852 0.0 38-39 0.0 0.0 0.0 0.1984933353314816 0.0 40-41 0.0 2.0756387674524897E-5 0.0 0.28116602743911423 0.0 42-43 0.0 8.302555069809959E-5 0.0 0.366848395759553 0.0 44-45 0.0 8.302555069809959E-5 0.0 0.4703605010924087 0.0 46-47 0.0 8.302555069809959E-5 0.0 0.5951479037916524 0.0 48-49 0.0 8.302555069809959E-5 0.0 0.7297945906362954 0.0 50-51 0.0 8.302555069809959E-5 0.0 0.8807765545807895 0.0 52-53 0.0 8.302555069809959E-5 0.0 1.022833271825238 0.0 54-55 0.0 8.302555069809959E-5 0.0 1.180104421235113 0.0 56-57 0.0 8.302555069809959E-5 0.0 1.364109797969776 0.0 58-59 0.0 8.302555069809959E-5 0.0 1.5637862473987056 0.0 60-61 0.0 8.302555069809959E-5 0.0 1.7867306074107776 0.0 62-63 0.0 8.302555069809959E-5 0.0 2.0060633559674823 0.0 64-65 0.0 8.302555069809959E-5 0.0 2.226122578092795 0.0 66-67 0.0 8.302555069809959E-5 0.0 2.483231952217135 0.0 68-69 0.0 8.302555069809959E-5 0.0 2.771455151465588 0.0 70-71 0.0 8.302555069809959E-5 0.0 3.079106329577396 0.0 72-73 0.0 8.302555069809959E-5 0.0 3.393918461436915 0.0 74-75 0.0 8.302555069809959E-5 0.0 3.7113666545310986 0.0 76-77 0.0 1.0378193837262448E-4 0.0 4.056088741029608 0.0 78-79 0.0 1.2453832604714937E-4 0.0 4.441244270718093 0.0 80-81 0.0 1.2453832604714937E-4 0.0 4.857907996896505 0.0 82-83 0.0 1.2453832604714937E-4 0.0 5.2863613512740475 0.0 84-85 0.0 1.2453832604714937E-4 0.0 5.71288436159786 0.0 86-87 0.0 1.2453832604714937E-4 0.0 6.1488307719259065 0.0 88-89 4.1512775349049794E-5 1.2453832604714937E-4 0.0 6.6212876681734425 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6100 0.0 56.80914 1 GTATCAA 11525 0.0 44.895756 1 TATCAAC 15215 0.0 33.73511 2 ATCAACG 15475 0.0 33.169006 3 TCAACGC 15720 0.0 32.682293 4 CAACGCA 16020 0.0 32.18559 5 AACGCAG 16575 0.0 31.130733 6 TGGTATC 2535 0.0 29.809143 2 ACGCAGA 18995 0.0 27.035618 7 CGCAGAG 19145 0.0 26.699728 8 GTGGTAT 3120 0.0 25.173637 1 TACATGG 15340 0.0 25.06418 2 GTACATG 15620 0.0 24.897612 1 GCAGAGT 20230 0.0 24.515257 9 ACATGGG 15750 0.0 23.235367 3 GAGTACT 10680 0.0 22.660696 12-13 CAGAGTA 19605 0.0 21.285519 10-11 AGTACTT 11180 0.0 21.052425 12-13 AGAGTAC 19370 0.0 20.844845 10-11 GTACTTT 11670 0.0 20.597574 14-15 >>END_MODULE