FastQCFastQC Report
Thu 26 May 2016
SRR938658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938658_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408897
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125960.5228949182966313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106840.443522491829248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74700.310100431857402No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26440.10975977802288765No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63550.060.0973471
GTATCAA113400.042.4026871
TATCAAC150000.032.183262
ATCAACG149900.031.9686763
TCAACGC154200.031.0772064
CAACGCA157900.030.3790935
AACGCAG164400.029.0923356
TGGTATC24600.028.614222
ACGCAGA189600.025.175537
CGCAGAG192000.024.8608368
GTGGTAT30400.024.5629961
GTACATG154700.023.1812231
GAGTACT105050.022.96775612-13
TACATGG153650.022.9062732
GCAGAGT207650.022.7126719
ACATGGG154350.021.9608253
GTACTTT112300.021.33694814-15
CAGAGTA198600.021.04526310-11
GGACCGA9750.020.4595286
AGTACTT109000.020.30534212-13
Produced by FastQC (version 0.11.5)