##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938658_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2408897 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.773117322990565 31.0 31.0 34.0 30.0 34.0 2 31.996134330359496 31.0 31.0 34.0 30.0 34.0 3 32.048025299545806 33.0 31.0 34.0 30.0 34.0 4 35.72990501461872 37.0 35.0 37.0 33.0 37.0 5 35.600943917485885 37.0 35.0 37.0 33.0 37.0 6 35.637445685722554 37.0 35.0 37.0 33.0 37.0 7 35.574755998284694 37.0 35.0 37.0 33.0 37.0 8 35.5720568376315 37.0 35.0 37.0 33.0 37.0 9 37.13908855380699 39.0 37.0 39.0 33.0 39.0 10-11 37.05209977844632 39.0 37.0 39.0 33.0 39.0 12-13 36.94258118964821 39.0 37.0 39.0 33.0 39.0 14-15 37.953786940662056 40.0 37.0 41.0 33.0 41.0 16-17 37.88220666969156 40.0 37.0 41.0 33.0 41.0 18-19 38.029984677634616 40.0 37.0 41.0 33.0 41.0 20-21 38.101693430644815 40.0 37.0 41.0 33.0 41.0 22-23 37.99694216896779 40.0 37.0 41.0 33.0 41.0 24-25 37.89391721605365 40.0 37.0 41.0 32.5 41.0 26-27 37.698656065410844 40.0 37.0 41.0 32.0 41.0 28-29 37.56620208336014 39.5 36.5 41.0 32.0 41.0 30-31 37.311342078968096 39.0 36.0 41.0 31.5 41.0 32-33 37.062348244860615 39.0 35.5 41.0 31.0 41.0 34-35 36.88731813772029 39.0 35.0 40.5 31.0 41.0 36-37 36.86322370777995 39.0 35.0 41.0 31.0 41.0 38-39 36.72749519800971 39.0 35.0 41.0 30.0 41.0 40-41 36.53712508255853 39.0 35.0 41.0 30.0 41.0 42-43 36.288383230997425 39.0 35.0 40.0 30.0 41.0 44-45 35.96394117307631 38.5 35.0 40.0 29.5 41.0 46-47 35.51900890739621 38.0 35.0 40.0 27.5 41.0 48-49 35.11153071301928 38.0 34.0 40.0 26.0 41.0 50-51 34.733074515016625 38.0 34.0 40.0 25.0 41.0 52-53 34.24653503242355 38.0 33.0 40.0 24.0 41.0 54-55 33.93760380788386 37.5 33.0 40.0 23.0 41.0 56-57 33.60489136729383 37.0 33.0 40.0 22.5 41.0 58-59 33.17424987452763 36.5 32.0 39.5 21.5 41.0 60-61 32.80943373668529 36.0 31.5 39.0 20.0 40.5 62-63 32.36163439117571 35.5 31.0 39.0 18.5 40.0 64-65 31.865403751177404 35.0 31.0 38.5 17.0 40.0 66-67 31.377003873557065 35.0 30.0 38.0 13.5 40.0 68-69 30.84410230076255 34.5 29.0 37.5 9.5 40.0 70-71 30.283897360493206 34.0 29.0 37.0 7.5 39.0 72-73 29.854617486758464 34.0 29.0 36.0 7.0 39.0 74-75 29.34590623841534 34.0 28.0 36.0 4.0 39.0 76-77 28.07875596175345 31.5 26.5 34.5 2.0 36.5 78-79 28.784440347594767 33.0 28.0 35.0 2.0 37.0 80-81 28.513354037138157 33.0 27.0 35.0 2.0 37.0 82-83 28.056484980470316 33.0 26.5 35.0 2.0 36.0 84-85 27.536529789360024 33.0 26.0 35.0 2.0 36.0 86-87 26.970438752673942 32.5 25.5 35.0 2.0 35.5 88-89 26.339641130359663 32.0 24.5 34.0 2.0 35.0 90-91 25.810095865452112 32.0 23.5 34.0 2.0 35.0 92-93 25.078708429625674 31.0 20.0 34.0 2.0 35.0 94-95 24.312571687373932 31.0 16.0 34.0 2.0 35.0 96-97 23.32637406248586 31.0 2.0 34.0 2.0 35.0 98-99 22.100475860943828 30.0 2.0 34.0 2.0 35.0 100-101 18.49566917971171 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.345930141960924 1101 2 0.3811009366843834 1101 3 0.34266066634641845 1101 4 0.2561210315686324 1101 5 0.22816951865816293 1101 6 0.2141604777337207 1101 7 0.26579482689968614 1101 8 0.31889886971273995 1101 9 0.28672922113077703 1101 10-11 0.35981720088044966 1101 12-13 0.45002696043781043 1101 14-15 0.6325903953362015 1101 16-17 0.4823395888600146 1101 18-19 0.4752293554488034 1101 20-21 0.5093033086404688 1101 22-23 0.49702419164001554 1101 24-25 0.4807182746741958 1101 26-27 0.4274714454486528 1101 28-29 0.4291517376958822 1101 30-31 0.29985085712534243 1101 32-33 0.41348117984533417 1101 34-35 0.3236937072460222 1101 36-37 0.5004714482975601 1101 38-39 0.6650783017230921 1101 40-41 0.8750784668604084 1101 42-43 0.7995812400871998 1101 44-45 0.7590301508922863 1101 46-47 0.65830938192844 1101 48-49 0.7999054413019024 1101 50-51 0.8716142200889081 1101 52-53 0.8069293517942597 1101 54-55 0.8127966459248626 1101 56-57 0.7312717639904562 1101 58-59 0.7463535624404116 1101 60-61 0.837869807382674 1101 62-63 0.7889834300224479 1101 64-65 0.9679161470994693 1101 66-67 0.7534382527875714 1101 68-69 0.7477795287821252 1101 70-71 0.7526513816400531 1101 72-73 0.5356185302166452 1101 74-75 0.6106353810696952 1101 76-77 0.6073049371687027 1101 78-79 0.48945823365794894 1101 80-81 0.6328577592294273 1101 82-83 0.565085105926336 1101 84-85 0.745126746695167 1101 86-87 0.6191065822960553 1101 88-89 0.6336147193881239 1101 90-91 0.6813278707002475 1101 92-93 0.7818709845170808 1101 94-95 0.8829865227779514 1101 96-97 0.8682777518662732 1101 98-99 0.60249515584146 1101 100-101 0.4193674092566191 1102 1 0.3760660495426471 1102 2 0.3401527390585102 1102 3 0.30611613127209836 1102 4 0.22415608215350602 1102 5 0.20164980828903367 1102 6 0.24550271519640887 1102 7 0.3531197066157574 1102 8 0.30139591671771626 1102 9 0.3568192211587018 1102 10-11 0.3682377743897831 1102 12-13 0.373631652837652 1102 14-15 0.4924564703799703 1102 16-17 0.45695081275427185 1102 18-19 0.4027937139856803 1102 20-21 0.4463730998504545 1102 22-23 0.477327784660055 1102 24-25 0.4243363148907733 1102 26-27 0.41160625281284524 1102 28-29 0.4529348663906134 1102 30-31 0.38143170775545343 1102 32-33 0.4698114796685715 1102 34-35 0.49084998002178537 1102 36-37 0.45116131819823835 1102 38-39 0.4654614231575067 1102 40-41 0.5314392041737008 1102 42-43 0.5602560029400578 1102 44-45 0.4508687149129358 1102 46-47 0.449593369295485 1102 48-49 0.6826889916536061 1102 50-51 0.7834446302131397 1102 52-53 0.6732543180756565 1102 54-55 0.7086822791503238 1102 56-57 0.7212385619944257 1102 58-59 0.8834926059213402 1102 60-61 0.8462610603740686 1102 62-63 0.8267482571291325 1102 64-65 0.7938011380659553 1102 66-67 0.7563381916333434 1102 68-69 0.728976699491767 1102 70-71 0.5806530347587788 1102 72-73 0.35240438711576516 1102 74-75 0.5160850880041359 1102 76-77 0.32717950120052564 1102 78-79 0.4022073533443056 1102 80-81 0.6669714837450123 1102 82-83 0.834465270177084 1102 84-85 0.7338313662125806 1102 86-87 0.5437598322660371 1102 88-89 0.4367148754849772 1102 90-91 0.23858386116130603 1102 92-93 0.31353295524682423 1102 94-95 0.4926550333794104 1102 96-97 0.508503827021741 1102 98-99 0.7079959642500278 1102 100-101 0.22987169424088094 1103 1 0.36672991196737925 1103 2 0.33957462574829833 1103 3 0.31067040852583006 1103 4 0.28106231269777027 1103 5 0.26766982513541393 1103 6 0.24329751374615682 1103 7 0.3107683288226326 1103 8 0.27284862914166297 1103 9 0.31574963305789794 1103 10-11 0.43254045463819324 1103 12-13 0.42864135781675117 1103 14-15 0.5605953640646746 1103 16-17 0.5198749151091135 1103 18-19 0.483809362283516 1103 20-21 0.4695277150781223 1103 22-23 0.5096602756744559 1103 24-25 0.5701241970079778 1103 26-27 0.5745452657824757 1103 28-29 0.5400246282916399 1103 30-31 0.564333941208595 1103 32-33 0.5352638467940736 1103 34-35 0.5277289005979142 1103 36-37 0.6100221472284133 1103 38-39 0.5500516951585084 1103 40-41 0.5195133282404782 1103 42-43 0.6271104910351113 1103 44-45 0.7115546555111365 1103 46-47 0.793179712218695 1103 48-49 0.7894228345637444 1103 50-51 0.795553618959552 1103 52-53 0.8898924714614154 1103 54-55 0.8042030781500031 1103 56-57 0.9060085461420826 1103 58-59 0.890049352582146 1103 60-61 1.0032685261450496 1103 62-63 0.9618881291594263 1103 64-65 0.9140303953080711 1103 66-67 0.9087272906659827 1103 68-69 0.8101205267588334 1103 70-71 0.5859711540332313 1103 72-73 0.5223016869865376 1103 74-75 0.8349577408902569 1103 76-77 0.7047267312268417 1103 78-79 0.7602295256900824 1103 80-81 0.7683518927667166 1103 82-83 0.8906900594665537 1103 84-85 0.6712264033321631 1103 86-87 0.8091740072904976 1103 88-89 0.753384942818176 1103 90-91 0.6810851298715157 1103 92-93 0.6865036728920977 1103 94-95 0.7109307384945396 1103 96-97 0.5063077812121506 1103 98-99 0.09819498171898644 1103 100-101 0.043664274509691126 1104 1 0.2596402833316027 1104 2 0.2086866664042617 1104 3 0.27486138995818976 1104 4 0.13192183666252788 1104 5 0.05682709245657236 1104 6 0.10370050460817026 1104 7 0.1305605423785039 1104 8 0.15647918424922125 1104 9 0.18400781166090496 1104 10-11 0.2610179837112483 1104 12-13 0.173122137627864 1104 14-15 0.35403395176654584 1104 16-17 0.34462644868653314 1104 18-19 0.3064385367996465 1104 20-21 0.2513310740215928 1104 22-23 0.22126650858148622 1104 24-25 0.26377444870569633 1104 26-27 0.35260404214610475 1104 28-29 0.41945650405442336 1104 30-31 0.34896368077208706 1104 32-33 0.3728001487993069 1104 34-35 0.40225578781605265 1104 36-37 0.4263022597346051 1104 38-39 0.2528390714972133 1104 40-41 0.22582595956537688 1104 42-43 0.3323290579880762 1104 44-45 0.3940898308531118 1104 46-47 0.21214660204476132 1104 48-49 0.16954472008331578 1104 50-51 0.20225675062588522 1104 52-53 0.20177345006853642 1104 54-55 0.2524431958262596 1104 56-57 0.11075900452817677 1104 58-59 0.07599883231537063 1104 60-61 -0.01843918652264165 1104 62-63 0.08742586780329731 1104 64-65 0.08396896070543747 1104 66-67 0.17599126043252156 1104 68-69 0.10670433506365384 1104 70-71 0.11930437298450869 1104 72-73 -0.06567012469941957 1104 74-75 -0.04245557752765805 1104 76-77 -0.048642678207162504 1104 78-79 0.007154650051973022 1104 80-81 -0.045747239874149415 1104 82-83 0.0018485438127946452 1104 84-85 0.045227419044827855 1104 86-87 0.009287131144532168 1104 88-89 0.0913507494461605 1104 90-91 -0.1042103132617278 1104 92-93 -0.05095776605704927 1104 94-95 0.12812613621810343 1104 96-97 0.06610471874696344 1104 98-99 -0.01491257287270642 1104 100-101 0.016483666386047702 1105 1 0.2801818398495861 1105 2 0.30841232228464577 1105 3 0.19069193622351577 1105 4 0.26185076462564894 1105 5 0.18576846812160852 1105 6 0.2501162197777518 1105 7 0.2492750370070027 1105 8 0.3303583358267801 1105 9 0.3464938763208778 1105 10-11 0.29459980957610554 1105 12-13 0.3518071787524377 1105 14-15 0.45499431843364135 1105 16-17 0.3367597419264712 1105 18-19 0.4016533249175893 1105 20-21 0.34253832515769744 1105 22-23 0.3807175355060721 1105 24-25 0.4288582914991892 1105 26-27 0.5045009610117788 1105 28-29 0.4794779785395846 1105 30-31 0.4692973249013619 1105 32-33 0.49036322018429956 1105 34-35 0.4857330715893369 1105 36-37 0.4722857269769989 1105 38-39 0.5938182734078481 1105 40-41 0.5059672166808724 1105 42-43 0.56137412844717 1105 44-45 0.5211090129478038 1105 46-47 0.24644827024737737 1105 48-49 0.32056351422390605 1105 50-51 0.3081819201935474 1105 52-53 0.25921021643508624 1105 54-55 0.35341645223219587 1105 56-57 0.3911169534768817 1105 58-59 0.31931789138966593 1105 60-61 0.34770448124442055 1105 62-63 0.46389674023850347 1105 64-65 0.28915052754964066 1105 66-67 0.10212097843748325 1105 68-69 0.28192068437233075 1105 70-71 0.23558144504157497 1105 72-73 0.295934101548486 1105 74-75 0.35006076630062566 1105 76-77 0.3286575542768553 1105 78-79 0.3478756064958368 1105 80-81 0.55554483475726 1105 82-83 0.6300511481529831 1105 84-85 0.6406719690108638 1105 86-87 0.4930075712777331 1105 88-89 0.4149435865732194 1105 90-91 0.5401363734804789 1105 92-93 0.6184992135832914 1105 94-95 0.6662415446299299 1105 96-97 0.7165295933829867 1105 98-99 0.6500488624709213 1105 100-101 0.4131478019767272 1106 1 0.10299881186672621 1106 2 0.09339353148652663 1106 3 0.12182155767307279 1106 4 0.09370432260718076 1106 5 0.047638573223871106 1106 6 3.3725705785769833E-4 1106 7 0.09825644253065491 1106 8 0.17161745953053043 1106 9 0.04103087245754722 1106 10-11 0.024580948663064817 1106 12-13 0.04789520452668938 1106 14-15 0.14137098979801266 1106 16-17 -0.057725193883321424 1106 18-19 0.21762287400854774 1106 20-21 0.1530890427838827 1106 22-23 0.28564635133521676 1106 24-25 0.23926404653169442 1106 26-27 0.05945203832749968 1106 28-29 0.18094934010118635 1106 30-31 0.20479098651274796 1106 32-33 0.30127099803218726 1106 34-35 0.09150782390839396 1106 36-37 -0.3180789932995296 1106 38-39 -0.04583662096403174 1106 40-41 0.12859417726927092 1106 42-43 0.232704138333375 1106 44-45 0.08695489685152324 1106 46-47 -0.21998473845388133 1106 48-49 -0.21264913380620953 1106 50-51 -0.22009402407910983 1106 52-53 -0.043632154711225724 1106 54-55 -0.35551837945694587 1106 56-57 -0.15491015996204993 1106 58-59 -0.04893573881022917 1106 60-61 -0.2666409434065784 1106 62-63 -0.22608488965288842 1106 64-65 -0.4732124854286326 1106 66-67 -0.24524620637842887 1106 68-69 -0.8702305289663315 1106 70-71 -0.7659809782702354 1106 72-73 -0.6287028784617483 1106 74-75 -0.361424933761775 1106 76-77 -0.40361730672031726 1106 78-79 -0.42043043460553164 1106 80-81 -1.0312118594645696 1106 82-83 -0.5077025802078019 1106 84-85 -0.4987803808906257 1106 86-87 -0.31519859112243864 1106 88-89 -0.36827563473049807 1106 90-91 -0.4425848566156496 1106 92-93 -0.5564974559580875 1106 94-95 -0.17419513108155726 1106 96-97 -0.11005693193200727 1106 98-99 -0.2516042720165039 1106 100-101 -0.40463772349952976 1107 1 0.20520530872290976 1107 2 0.20127574547724691 1107 3 0.2230154616628397 1107 4 0.19525967530886845 1107 5 0.17833933275234415 1107 6 0.21303322688795134 1107 7 0.21423470531359357 1107 8 0.2169221049679564 1107 9 0.2765041464603186 1107 10-11 0.28380145333331797 1107 12-13 0.23993629165577346 1107 14-15 0.3209101594550887 1107 16-17 0.27668527672926757 1107 18-19 0.32225515107675307 1107 20-21 0.38421780721102294 1107 22-23 0.39335136157431094 1107 24-25 0.38224081808721877 1107 26-27 0.37864519963740406 1107 28-29 0.3467594940270402 1107 30-31 0.2859643871734505 1107 32-33 0.34621285357508924 1107 34-35 0.23496289471025733 1107 36-37 0.2281356445350653 1107 38-39 0.17189473513085574 1107 40-41 0.2533700949944304 1107 42-43 0.2597630003231757 1107 44-45 0.10101275379260244 1107 46-47 0.18365950615434912 1107 48-49 0.20273213506988697 1107 50-51 0.20152491744281065 1107 52-53 0.3577946121082576 1107 54-55 0.13146200658569995 1107 56-57 0.0854357142668789 1107 58-59 -0.03208126466697081 1107 60-61 0.06520652724876896 1107 62-63 0.19009622699898188 1107 64-65 0.23389838091665283 1107 66-67 0.29706072829005237 1107 68-69 0.2879462841658622 1107 70-71 0.09073511935780232 1107 72-73 0.19384650849206153 1107 74-75 0.14393635987590514 1107 76-77 0.16928326051609943 1107 78-79 0.24652643044719014 1107 80-81 0.37157293856590456 1107 82-83 0.3172989914905635 1107 84-85 0.36682323091412883 1107 86-87 0.43347795503058606 1107 88-89 0.14605599110973344 1107 90-91 0.11362838135960018 1107 92-93 0.12532558002667926 1107 94-95 1.4343537865002531E-4 1107 96-97 0.23444048922531735 1107 98-99 0.09285785927353629 1107 100-101 -0.3079572342696437 1108 1 0.15090570936359526 1108 2 0.18541448103131586 1108 3 0.12959244610267007 1108 4 0.12508122921577325 1108 5 0.1629499643536505 1108 6 0.12722867944897587 1108 7 0.09619852755854197 1108 8 0.08937642123908773 1108 9 0.1573097354928592 1108 10-11 0.1375647772145996 1108 12-13 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-0.373578819238773 2316 70-71 -0.3240802917297856 2316 72-73 -0.3405445844462207 2316 74-75 -0.12541313765057893 2316 76-77 -0.1394106021298782 2316 78-79 0.047217983363129434 2316 80-81 -0.26392319362046024 2316 82-83 -0.08714356653398525 2316 84-85 -0.059103826624355094 2316 86-87 0.10280919387964715 2316 88-89 0.16886340010792722 2316 90-91 0.2856356989322073 2316 92-93 0.30384763189659836 2316 94-95 0.17055484465769766 2316 96-97 0.20592419552237118 2316 98-99 0.37690933787386527 2316 100-101 0.44611189487516967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 10.0 4 39.0 5 86.0 6 218.0 7 545.0 8 1217.0 9 2209.0 10 3534.0 11 5517.0 12 7733.0 13 10942.0 14 14295.0 15 19087.0 16 23896.0 17 30380.0 18 27934.0 19 21457.0 20 20512.0 21 21525.0 22 23478.0 23 26357.0 24 30283.0 25 35845.0 26 42747.0 27 51855.0 28 61756.0 29 73276.0 30 87640.0 31 103727.0 32 125408.0 33 155013.0 34 196518.0 35 257025.0 36 336516.0 37 386703.0 38 195045.0 39 8564.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45967318435406 19.80172536542901 15.59873650877731 22.139864941439612 2 15.837438771450238 20.959455631780617 39.601218050471346 23.601887546297796 3 19.18527417675571 25.446970464331205 30.004059971339426 25.363695387573664 4 13.24236777247014 17.396302124997458 36.357303778451296 33.0040263240811 5 13.542792802150547 38.09828695836432 33.5064794771546 14.852440762330534 6 31.543814451178275 36.35684713792246 17.280190892346166 14.819147518553097 7 28.48112642425143 33.47295463442397 20.056814384342708 17.98910455698189 8 26.752742022593743 34.993027929380126 19.21194638043885 19.04228366758728 9 27.019918244740225 16.60199668146874 19.926339731420644 36.45174534237039 10-11 25.132996554024515 27.07043929234002 26.49938955463849 21.297174598996968 12-13 26.213615609135633 24.524087165204655 27.285537737811122 21.97675948784859 14-15 23.024832527086048 25.653753564390673 26.20967189547747 25.111742013045806 16-17 21.87102644903456 28.952254911687795 26.705791073673968 22.470927565603677 18-19 21.850560650787475 26.963938267182037 29.774872068004566 21.410629014025922 20-21 22.995649046015664 25.8143249794408 29.95451860332758 21.235507371215956 22-23 22.60515912469483 25.90889108168593 29.77626274597876 21.70968704764048 24-25 22.110019876358095 26.19289283205723 29.972140718637448 21.724946572947232 26-27 21.7615988757386 26.56478566691265 29.899582449814577 21.77403300753417 28-29 21.732394535756406 26.58557007626312 29.913088853529228 21.768946534451246 30-31 21.92254380324273 26.325181192886205 29.821304107232482 21.930970896638584 32-33 21.771914697888704 26.351873907435643 29.705940104537472 22.17027129013818 34-35 22.258076971300795 26.513054265522673 29.58946093186274 21.639407831313797 36-37 22.256449533914928 26.165420244551964 29.51068851070719 22.067441710825918 38-39 21.87382526819951 26.433388153285804 29.50320170525132 22.189584873263367 40-41 22.28530649032224 26.18486771884916 29.54896916311264 21.98085662771596 42-43 22.003963241937715 26.339078242167073 29.337496806096926 22.319461709798283 44-45 22.01034789165911 26.552137046983958 29.270580948579568 22.166934112777366 46-47 22.278536608248505 26.765465688238226 28.581317507556363 22.374680195956905 48-49 22.21240675711747 26.758948182508423 28.624449281144027 22.40419577923008 50-51 22.450565549294968 27.022969433728385 28.172105324553105 22.354359692423547 52-53 22.473667408776716 26.996048398914525 28.089577927159194 22.44070626514957 54-55 22.25587063290792 27.07195450864026 28.20969514263167 22.462479715820145 56-57 22.229676071662674 27.221234448795446 28.00846196412715 22.540627515414734 58-59 22.346534534270248 26.860405405461503 28.006884478663885 22.78617558160436 60-61 22.18355537824988 27.041214298494292 28.218661902107062 22.556568421148768 62-63 22.08797221300869 27.010785434163438 28.30249695192447 22.5987454009034 64-65 21.987843398866787 26.97427494824395 28.15153574436765 22.88634590852162 66-67 22.069611971625175 27.01621406652674 28.00616963123356 22.908004330614524 68-69 22.160051725336803 26.913323736885747 28.013109840579276 22.913514697198174 70-71 22.14503611923551 26.89885181866469 27.900991179986356 23.05512088211345 72-73 22.225026640823582 26.968463159695084 27.68501517499503 23.12149502448631 74-75 22.017877891831823 27.29404785675768 27.555433046742973 23.132641204667532 76-77 22.05544695352271 26.97161813062161 27.90625751121779 23.06667740463789 78-79 22.131145499371705 26.989219547369604 27.8688545006283 23.010780452630396 80-81 21.927962473683195 27.253572682123295 27.691890324486234 23.126574519707273 82-83 22.101779123353023 27.155241661799266 27.564020336396577 23.178958878451134 84-85 22.118048139137166 26.973580287351922 27.56713592745897 23.341235646051945 86-87 21.95970601690602 27.073295677681347 27.554397416854375 23.412600888558256 88-89 22.03958415753626 27.116438961796973 27.401403881367912 23.442572999298847 90-91 22.063919691782637 26.94892664228266 27.511712687480323 23.475440978454383 92-93 21.840743849616047 26.84002982306256 27.474683192230064 23.844543135091335 94-95 21.81368295989778 26.87266438456819 27.498428739496216 23.815223916037816 96-97 21.939273451708395 26.71278597632028 27.36038527176546 23.987555300205862 98-99 21.768874381536584 26.6909267678293 27.27681817398735 24.26338067664677 100-101 21.902742209401232 26.614193134866287 27.40484545416429 24.07821920156819 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1540.0 1 1014.5 2 693.5 3 870.5 4 763.5 5 775.5 6 1310.0 7 2729.0 8 4254.0 9 4746.5 10 3920.0 11 3060.0 12 3046.5 13 3238.0 14 3412.0 15 3612.0 16 3872.5 17 4094.5 18 4395.0 19 4747.5 20 5118.5 21 5719.0 22 6537.0 23 7669.0 24 9257.5 25 11396.5 26 14454.0 27 17686.0 28 20202.0 29 23176.5 30 26851.5 31 31251.5 32 36591.0 33 41191.0 34 45128.0 35 48809.0 36 53493.0 37 58085.5 38 61098.0 39 63569.0 40 66762.5 41 71920.0 42 76402.0 43 80484.5 44 83926.0 45 86261.5 46 88066.5 47 90080.0 48 91709.5 49 90802.0 50 89601.5 51 86326.0 52 81599.0 53 80439.0 54 80589.0 55 75422.0 56 67823.0 57 61851.0 58 57182.5 59 51914.5 60 43578.5 61 35589.0 62 29290.5 63 22563.0 64 17153.5 65 13764.0 66 10348.0 67 7948.5 68 6163.0 69 4547.0 70 3516.0 71 2680.5 72 2259.5 73 2097.0 74 1695.0 75 1237.0 76 905.0 77 653.0 78 471.5 79 302.5 80 174.0 81 95.5 82 47.0 83 16.5 84 8.5 85 9.0 86 7.5 87 3.0 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.642044055847967E-4 2 0.04628674451419052 3 5.396660795376473E-4 4 0.0 5 0.08580690664648592 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.0756387674524898E-4 26-27 0.008489362558880683 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010751808815403897 36-37 8.302555069809959E-5 38-39 0.004255059473277604 40-41 0.005230609693980274 42-43 4.358841411650228E-4 44-45 2.0756387674524897E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.1512775349049794E-5 68-69 8.094991193064709E-4 70-71 2.0756387674524897E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0011416013220988694 82-83 5.81178854886697E-4 84-85 0.004504136125371903 86-87 0.0021171515428015392 88-89 1.6605110139619918E-4 90-91 0.0032172400895513586 92-93 9.547938330281453E-4 94-95 1.2453832604714937E-4 96-97 0.0 98-99 1.4529471372167426E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2408897.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.19582709107588 #Duplication Level Percentage of deduplicated Percentage of total 1 72.27655343043018 35.5570482530235 2 14.570691659765423 14.33634454982402 3 5.397516542971929 7.96605871707806 4 2.6300464358845304 5.175492388051029 5 1.399776525122932 3.4431581948047394 6 0.8413977219491081 2.4835954106300075 7 0.5479472901971651 1.8869704100494318 8 0.37886611508326756 1.4910905510643269 9 0.27443397562637406 1.2150905771548468 >10 1.4914841116553987 13.555342300292903 >50 0.12202540444987767 4.180064998714104 >100 0.06461469539470101 5.658232002902939 >500 0.003801347565711022 1.263899652752093 >1k 5.913207324438473E-4 0.5097073370270133 >5k 8.447439034912106E-5 0.31043732634258375 >10k+ 1.6894878069824212E-4 0.9674673302885342 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12596 0.5228949182966313 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10684 0.443522491829248 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7470 0.310100431857402 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2644 0.10975977802288765 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.1512775349049794E-5 0.0 10-11 0.0 0.0 0.0 8.302555069809959E-5 0.0 12-13 0.0 0.0 0.0 8.302555069809959E-5 0.0 14-15 0.0 0.0 0.0 1.0378193837262448E-4 0.0 16-17 0.0 0.0 0.0 2.0756387674524896E-4 0.0 18-19 0.0 0.0 0.0 2.490766520942988E-4 0.0 20-21 0.0 0.0 0.0 4.358841411650228E-4 0.0 22-23 0.0 0.0 0.0 7.472299562828963E-4 0.0 24-25 0.0 0.0 0.0 0.0015567290755893672 0.0 26-27 0.0 0.0 0.0 0.002636061234664662 0.0 28-29 0.0 0.0 0.0 0.006040108813286745 0.0 30-31 0.0 0.0 0.0 0.017933518950789512 0.0 32-33 0.0 0.0 0.0 0.039167303541828485 0.0 34-35 0.0 0.0 0.0 0.0716095374771109 0.0 36-37 0.0 0.0 0.0 0.1240609291306353 0.0 38-39 0.0 0.0 0.0 0.20177284458405653 0.0 40-41 0.0 0.0 0.0 0.28562865078913713 0.0 42-43 0.0 0.0 0.0 0.37263942792074545 0.0 44-45 0.0 0.0 0.0 0.47694027598523303 0.0 46-47 0.0 0.0 0.0 0.6018314606228494 0.0 48-49 0.0 0.0 0.0 0.7362705835907472 0.0 50-51 0.0 0.0 0.0 0.8874601114119864 0.0 52-53 0.0 0.0 0.0 1.0301395202866706 0.0 54-55 0.0 0.0 0.0 1.1883447071418993 0.0 56-57 0.0 0.0 0.0 1.3729727755067982 0.0 58-59 0.0 0.0 0.0 1.573687044319454 0.0 60-61 0.0 0.0 0.0 1.796195520190361 0.0 62-63 0.0 0.0 0.0 2.0159226401128816 0.0 64-65 0.0 0.0 0.0 2.2367498485821518 0.0 66-67 0.0 0.0 0.0 2.495519733720454 0.0 68-69 0.0 0.0 0.0 2.785569495084265 0.0 70-71 0.0 0.0 0.0 3.095130260862129 0.0 72-73 0.0 0.0 0.0 3.4127860178330582 0.0 74-75 0.0 0.0 0.0 3.734987423704708 0.0 76-77 0.0 0.0 0.0 4.087202566153721 0.0 78-79 0.0 0.0 0.0 4.479975690118756 0.0 80-81 0.0 0.0 0.0 4.907847865641411 0.0 82-83 0.0 0.0 0.0 5.347385131037151 0.0 84-85 0.0 0.0 0.0 5.786361973965677 0.0 86-87 0.0 0.0 0.0 6.238187020864736 0.0 88-89 0.0 0.0 0.0 6.727684911393057 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6355 0.0 60.097347 1 GTATCAA 11340 0.0 42.402687 1 TATCAAC 15000 0.0 32.18326 2 ATCAACG 14990 0.0 31.968676 3 TCAACGC 15420 0.0 31.077206 4 CAACGCA 15790 0.0 30.379093 5 AACGCAG 16440 0.0 29.092335 6 TGGTATC 2460 0.0 28.61422 2 ACGCAGA 18960 0.0 25.17553 7 CGCAGAG 19200 0.0 24.860836 8 GTGGTAT 3040 0.0 24.562996 1 GTACATG 15470 0.0 23.181223 1 GAGTACT 10505 0.0 22.967756 12-13 TACATGG 15365 0.0 22.906273 2 GCAGAGT 20765 0.0 22.712671 9 ACATGGG 15435 0.0 21.960825 3 GTACTTT 11230 0.0 21.336948 14-15 CAGAGTA 19860 0.0 21.045263 10-11 GGACCGA 975 0.0 20.459528 6 AGTACTT 10900 0.0 20.305342 12-13 >>END_MODULE