FastQCFastQC Report
Thu 26 May 2016
SRR938657_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938657_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2406201
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131800.5477514139508711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102640.4265645305608301No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71870.29868660182586576No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24850.1032748303238175No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59400.057.4406971
GTATCAA113700.045.9349441
TATCAAC152250.034.2106672
ATCAACG154450.033.78493
TGGTATC24200.033.580642
TCAACGC156900.033.257354
CAACGCA160350.032.5121655
AACGCAG165350.031.7569836
ACGCAGA187000.027.8688937
CGCAGAG188900.027.4879888
GTGGTAT30600.027.4084451
GCAGAGT198700.025.2226839
GTACATG151950.024.4930821
TACATGG151150.024.1154542
TACCGTG1852.5406007E-723.1113597
ACATGGG155200.022.6900143
GAGTACT109150.022.23787112-13
GCGTATC1100.00282494621.6605991
CAGAGTA193400.021.47872210-11
AGAGTAC191450.021.36253710-11
Produced by FastQC (version 0.11.5)