FastQCFastQC Report
Thu 26 May 2016
SRR938657_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938657_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2406201
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126230.5246028906147076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108540.4510845103962636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76740.3189259750120626No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26680.11088018000158756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58300.060.131611
GTATCAA113400.045.343661
TATCAAC152350.033.626152
ATCAACG153750.033.2737853
TCAACGC156550.032.6786654
CAACGCA161350.031.7949075
TGGTATC23850.031.7111972
AACGCAG166850.030.8281066
CTATACG700.0083546627.1685894
GTGGTAT28650.026.8964061
ACGCAGA193950.026.4716197
CGCAGAG195050.026.2005798
GCAGAGT209850.024.1716829
GTACATG156150.021.9632781
TACATGG153750.021.8420542
GAGTACT110700.021.68793112-13
ACATGGG154300.021.1687833
AGTACTT116500.021.03625112-13
AGAGTAC201450.020.85331710-11
CAGAGTA201650.020.70309310-11
Produced by FastQC (version 0.11.5)