##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938657_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2406201 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.784394986121274 31.0 31.0 34.0 30.0 34.0 2 32.01077840130563 31.0 31.0 34.0 30.0 34.0 3 32.06657132966032 33.0 31.0 34.0 30.0 34.0 4 35.749424092168525 37.0 35.0 37.0 33.0 37.0 5 35.622318750594815 37.0 35.0 37.0 33.0 37.0 6 35.67077978938584 37.0 35.0 37.0 33.0 37.0 7 35.60369561811336 37.0 35.0 37.0 33.0 37.0 8 35.59214878557527 37.0 35.0 37.0 33.0 37.0 9 37.14539724653094 39.0 37.0 39.0 33.0 39.0 10-11 37.087683655687954 39.0 37.0 39.0 33.0 39.0 12-13 36.976157020963754 39.0 37.0 39.0 33.0 39.0 14-15 37.97973340548026 40.0 37.0 41.0 33.0 41.0 16-17 37.90576099004198 40.0 37.0 41.0 33.0 41.0 18-19 37.946445247092825 40.0 37.0 41.0 33.0 41.0 20-21 37.957500017662696 40.0 37.0 41.0 33.0 41.0 22-23 37.97337462664174 40.0 37.0 41.0 33.0 41.0 24-25 37.89646334616269 40.0 37.0 41.0 33.0 41.0 26-27 37.71727798301139 40.0 37.0 41.0 32.0 41.0 28-29 37.57830517899377 39.5 36.5 41.0 32.0 41.0 30-31 37.30991779157269 39.0 36.0 41.0 31.5 41.0 32-33 37.05930967529313 39.0 35.5 41.0 31.0 41.0 34-35 36.867748995200316 39.0 35.0 40.5 31.0 41.0 36-37 36.85775149291352 39.0 35.0 41.0 31.0 41.0 38-39 36.6955474625769 39.0 35.0 41.0 30.0 41.0 40-41 36.49910335836449 39.0 35.0 40.5 30.0 41.0 42-43 36.235835659614466 39.0 35.0 40.0 30.0 41.0 44-45 35.898913266181836 38.0 35.0 40.0 29.0 41.0 46-47 35.45425776982056 38.0 34.5 40.0 27.5 41.0 48-49 35.00568302481796 38.0 34.0 40.0 26.0 41.0 50-51 34.617135684009774 38.0 33.5 40.0 24.5 41.0 52-53 34.105075802063084 38.0 33.0 40.0 23.5 41.0 54-55 33.78437171292008 37.0 33.0 40.0 23.0 41.0 56-57 33.42417736506634 37.0 32.0 40.0 22.0 41.0 58-59 32.97088917343148 36.5 31.5 39.0 20.5 41.0 60-61 32.56719243321734 36.0 31.0 39.0 18.5 40.0 62-63 32.090640599018954 35.5 31.0 39.0 17.5 40.0 64-65 31.59464795335053 35.0 30.0 38.5 13.5 40.0 66-67 31.10011923359686 35.0 29.5 38.0 9.5 40.0 68-69 30.540216299469577 34.0 29.0 37.5 7.5 40.0 70-71 29.986723054308428 34.0 28.5 37.0 6.5 39.0 72-73 29.411546250708064 34.0 28.0 36.0 2.0 39.0 74-75 28.799803507687017 33.5 26.5 36.0 2.0 38.5 76-77 27.52192855044113 31.5 26.0 34.5 2.0 36.5 78-79 28.14821621302626 33.0 26.0 35.0 2.0 37.0 80-81 27.856744303572313 33.0 26.0 35.0 2.0 37.0 82-83 27.36360158606866 33.0 26.0 35.0 2.0 36.0 84-85 26.770652368609273 32.0 25.0 35.0 2.0 36.0 86-87 26.130794975149623 32.0 23.5 34.5 2.0 35.0 88-89 25.39387046219331 31.0 20.5 34.0 2.0 35.0 90-91 24.846398326656832 31.0 19.0 34.0 2.0 35.0 92-93 24.066974039159653 31.0 13.0 34.0 2.0 35.0 94-95 23.28440475255392 30.5 4.0 34.0 2.0 35.0 96-97 22.313676829159327 30.0 2.0 34.0 2.0 35.0 98-99 21.146285991901756 29.5 2.0 34.0 2.0 35.0 100-101 18.05469846450899 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3033909610870076 1101 2 0.3460684022203111 1101 3 0.2912795349387167 1101 4 0.24173526064738127 1101 5 0.16496444422750045 1101 6 0.21372739065056834 1101 7 0.27601329595445634 1101 8 0.22232736518958518 1101 9 0.3087729354857771 1101 10-11 0.3439829345871104 1101 12-13 0.3369879656148882 1101 14-15 0.4350734193071233 1101 16-17 0.3813317676479784 1101 18-19 0.5048525689700512 1101 20-21 0.4017426058583311 1101 22-23 0.6271798842594549 1101 24-25 0.5524555790427925 1101 26-27 0.5598781301749653 1101 28-29 0.5593700178224736 1101 30-31 0.5727272958051088 1101 32-33 0.5508770453576943 1101 34-35 0.45810995986862224 1101 36-37 0.5994920481686208 1101 38-39 0.6601136406595671 1101 40-41 0.7604148679239486 1101 42-43 0.6123480469850477 1101 44-45 0.522352089525782 1101 46-47 0.7993476437119327 1101 48-49 0.8453960067093931 1101 50-51 0.8718541410912692 1101 52-53 0.8351375902859672 1101 54-55 0.9533018922102272 1101 56-57 1.1637738080325732 1101 58-59 1.227856304680465 1101 60-61 1.1973212358783911 1101 62-63 1.4129686437221807 1101 64-65 1.2984281712197863 1101 66-67 1.4769970424603898 1101 68-69 1.300080329628475 1101 70-71 1.5027917393705899 1101 72-73 1.509172339188325 1101 74-75 1.5220297540251622 1101 76-77 1.415714433044446 1101 78-79 1.61930303692521 1101 80-81 2.0406542042088773 1101 82-83 2.2490537639056782 1101 84-85 2.3191692083213837 1101 86-87 2.3809662136871665 1101 88-89 2.6461145016243925 1101 90-91 2.7430477142278065 1101 92-93 3.05676101347208 1101 94-95 3.127984250027705 1101 96-97 3.5280408912813996 1101 98-99 3.8317056557700937 1101 100-101 4.419908624069965 1102 1 0.36704179603693277 1102 2 0.34529261072466966 1102 3 0.31327703353064607 1102 4 0.23712315423060915 1102 5 0.18184570180629578 1102 6 0.22562964265380003 1102 7 0.3092573901767608 1102 8 0.3246848708640755 1102 9 0.42529491328653535 1102 10-11 0.37483027899481414 1102 12-13 0.43416634873899795 1102 14-15 0.6508397796544827 1102 16-17 0.5744416179054994 1102 18-19 0.6766130009196729 1102 20-21 0.6887934682553407 1102 22-23 0.4595198480414169 1102 24-25 0.4947994867201402 1102 26-27 0.5249978172262146 1102 28-29 0.4901796549537991 1102 30-31 0.6640089925373047 1102 32-33 0.40900937062386333 1102 34-35 0.6114609288936776 1102 36-37 0.8122069763970856 1102 38-39 1.0231765927626597 1102 40-41 0.857444867936934 1102 42-43 0.7890886750384851 1102 44-45 0.8166092193829755 1102 46-47 0.8580352746429796 1102 48-49 0.8420610124087773 1102 50-51 0.9659073875752071 1102 52-53 1.073995869370897 1102 54-55 0.9445954509329297 1102 56-57 1.1803511562196576 1102 58-59 1.346804146122885 1102 60-61 1.266941595902793 1102 62-63 1.4070124309180727 1102 64-65 1.3487104562893855 1102 66-67 1.3022955728290384 1102 68-69 1.518547132722464 1102 70-71 1.5832273991115606 1102 72-73 1.655602983991411 1102 74-75 1.8609223852614178 1102 76-77 1.9500179891739577 1102 78-79 2.2372347083965636 1102 80-81 2.346901793389179 1102 82-83 2.2380547981500314 1102 84-85 2.401878235858952 1102 86-87 2.545876346121524 1102 88-89 2.6382319058918497 1102 90-91 2.8299669907849214 1102 92-93 3.180027766693758 1102 94-95 3.359675116165139 1102 96-97 3.8296970459444424 1102 98-99 4.065900311034241 1102 100-101 4.683390490593133 1103 1 0.25585491761097146 1103 2 0.31075555005347155 1103 3 0.30635074167204834 1103 4 0.15444528062580787 1103 5 0.15551055647485867 1103 6 0.1512162027742292 1103 7 0.14052668522399614 1103 8 0.21845122889278912 1103 9 0.2588463364182161 1103 10-11 0.2815556425588923 1103 12-13 0.3533436357681552 1103 14-15 0.5412070627482848 1103 16-17 0.5708818997019449 1103 18-19 0.46417852877875276 1103 20-21 0.39740562427225257 1103 22-23 0.3725589474951292 1103 24-25 0.30753249450243914 1103 26-27 0.36398502766206775 1103 28-29 0.3674354663171968 1103 30-31 0.5000724333794935 1103 32-33 0.6071264079396812 1103 34-35 0.74798534967222 1103 36-37 0.7940514923443445 1103 38-39 0.8954939692096673 1103 40-41 0.9407355045252146 1103 42-43 0.9198160681729348 1103 44-45 1.0761064763370598 1103 46-47 1.3117923708420065 1103 48-49 1.4314551616401658 1103 50-51 1.3928257756193787 1103 52-53 1.6039834754115034 1103 54-55 1.6614913965328242 1103 56-57 1.6130008447973552 1103 58-59 1.822404069030675 1103 60-61 1.9418838750788439 1103 62-63 1.9302688562865455 1103 64-65 1.841029700007379 1103 66-67 1.8815801679055255 1103 68-69 1.9448353685633357 1103 70-71 2.062154769929837 1103 72-73 2.0006090381310884 1103 74-75 2.202508766521305 1103 76-77 1.882479087522622 1103 78-79 2.0543415189880037 1103 80-81 2.3337761411640514 1103 82-83 2.492941427682439 1103 84-85 2.6345039728565283 1103 86-87 2.764006408791033 1103 88-89 3.076575528881971 1103 90-91 3.2552980949858856 1103 92-93 3.3775589529138763 1103 94-95 3.394946974880213 1103 96-97 3.743868514830467 1103 98-99 4.028112501332753 1103 100-101 4.401885933892995 1104 1 0.2738638224665415 1104 2 0.243171773699288 1104 3 0.2975178063363302 1104 4 0.21613491109223304 1104 5 0.20807352858539474 1104 6 0.1880919877572822 1104 7 0.243209485412045 1104 8 0.24252501710504504 1104 9 0.2838993313015479 1104 10-11 0.3555955756360376 1104 12-13 0.4065857570915483 1104 14-15 0.5841255118283684 1104 16-17 0.5809772398164483 1104 18-19 0.5690727300197551 1104 20-21 0.4954923511102294 1104 22-23 0.3028482617464121 1104 24-25 0.4186317163605793 1104 26-27 0.4609099211070742 1104 28-29 0.45236644238198664 1104 30-31 0.5117933852057988 1104 32-33 0.5199791581772715 1104 34-35 0.4473072139694594 1104 36-37 0.567137205672239 1104 38-39 0.6607572362516478 1104 40-41 0.6796366919692289 1104 42-43 0.6898854195498672 1104 44-45 0.6568295339254817 1104 46-47 0.5302386832635122 1104 48-49 0.6373827728514598 1104 50-51 0.7320282094469093 1104 52-53 0.8583280228441552 1104 54-55 0.934037025926493 1104 56-57 1.0077587367275527 1104 58-59 1.1299056614109517 1104 60-61 1.0920157093527934 1104 62-63 1.3425788913617893 1104 64-65 1.339459733897602 1104 66-67 1.2026092938466277 1104 68-69 1.4020066735979135 1104 70-71 1.3216242675640721 1104 72-73 1.5369551666394905 1104 74-75 1.5581648544815039 1104 76-77 1.2803580058111024 1104 78-79 1.667805240394351 1104 80-81 1.7930753301220967 1104 82-83 2.231926132255939 1104 84-85 2.263421968975564 1104 86-87 2.3999441317145234 1104 88-89 2.49734466999454 1104 90-91 2.522310933819849 1104 92-93 2.775175917825379 1104 94-95 2.9563194936320976 1104 96-97 3.3668281436051117 1104 98-99 3.8152477059175602 1104 100-101 4.041008125008251 1105 1 0.23962918798689614 1105 2 0.23373428414628705 1105 3 0.261205501896832 1105 4 0.12916675501332975 1105 5 0.0038108594906276494 1105 6 0.19664972997372132 1105 7 0.19798094171645886 1105 8 0.24629180195110223 1105 9 0.3885614056871134 1105 10-11 0.326562447283834 1105 12-13 0.3483519818317973 1105 14-15 0.5084727393046364 1105 16-17 0.4764049729714799 1105 18-19 0.4529438822248437 1105 20-21 0.3819217352577766 1105 22-23 0.3558185820037636 1105 24-25 0.34631171999686217 1105 26-27 0.42563315259499035 1105 28-29 0.4440051770707214 1105 30-31 0.5564640224654411 1105 32-33 0.5903046940167087 1105 34-35 0.5406344770461686 1105 36-37 0.6109955428911249 1105 38-39 0.635180415247973 1105 40-41 0.5903181442561376 1105 42-43 0.6202471863171155 1105 44-45 0.6614207153381741 1105 46-47 0.9591400694520615 1105 48-49 1.0873878313986438 1105 50-51 0.9937885985298323 1105 52-53 1.0042414498412668 1105 54-55 1.0791682109168264 1105 56-57 1.0919461581001428 1105 58-59 1.2602592905153358 1105 60-61 1.2015589152107395 1105 62-63 1.2884465714973885 1105 64-65 1.4452104956121623 1105 66-67 1.5573732722986833 1105 68-69 1.4912713367866424 1105 70-71 1.3999254461322543 1105 72-73 1.4571609835288797 1105 74-75 1.7455686321165693 1105 76-77 1.6755463743783174 1105 78-79 1.9225547763276296 1105 80-81 2.1682083919067523 1105 82-83 2.2536899816783844 1105 84-85 2.4290124114004996 1105 86-87 2.724894939175954 1105 88-89 2.6105934449463746 1105 90-91 2.7926921136783314 1105 92-93 3.0873066072247184 1105 94-95 3.2854930883090425 1105 96-97 3.8109432937339918 1105 98-99 4.10764786192609 1105 100-101 4.289863720347938 1106 1 0.15525212858712933 1106 2 0.1331433143359746 1106 3 0.0659656764448826 1106 4 0.08421226218226252 1106 5 0.018143035107335947 1106 6 0.11299757929244691 1106 7 0.18412182692440382 1106 8 -5.681143370779296E-4 1106 9 0.20920171203674442 1106 10-11 0.2161557325274046 1106 12-13 0.12320958268672655 1106 14-15 0.02762181758409099 1106 16-17 0.002176546575540783 1106 18-19 0.2064079484818535 1106 20-21 0.4101109773986593 1106 22-23 0.2527118939638697 1106 24-25 0.05501427587896046 1106 26-27 0.11226393004623247 1106 28-29 0.3078151334337065 1106 30-31 0.3706527302755731 1106 32-33 0.38466127073770195 1106 34-35 0.2377577293393358 1106 36-37 0.3253299448859579 1106 38-39 0.33230188643863556 1106 40-41 0.40244130749417906 1106 42-43 0.5140706790591238 1106 44-45 -0.11940589433320525 1106 46-47 -0.31329264940089985 1106 48-49 0.25031436092042014 1106 50-51 0.09575155159777893 1106 52-53 0.38187641288337915 1106 54-55 0.4595786659885164 1106 56-57 0.2758510472265101 1106 58-59 0.8833451396559511 1106 60-61 0.6205595385966163 1106 62-63 0.7127825764844715 1106 64-65 0.28732929514907823 1106 66-67 0.4817097225613942 1106 68-69 0.47511157189217457 1106 70-71 0.7339172530607421 1106 72-73 1.1722563615678858 1106 74-75 1.5108002644970142 1106 76-77 1.527870000703011 1106 78-79 1.6902245599236778 1106 80-81 1.6204931901968926 1106 82-83 1.941051189265881 1106 84-85 2.066734292244739 1106 86-87 2.2486757745130284 1106 88-89 2.472989384032669 1106 90-91 2.3571352519809388 1106 92-93 2.219316937619233 1106 94-95 2.6652303756899265 1106 96-97 3.3919710789330324 1106 98-99 3.9553279759166493 1106 100-101 4.297171583915489 1107 1 0.11617318121870923 1107 2 0.1274361836059228 1107 3 0.09105990394912311 1107 4 0.15416811956766452 1107 5 0.08671094682210878 1107 6 0.01640165399533089 1107 7 0.09837734475122772 1107 8 0.15435972946597332 1107 9 0.2139427986241813 1107 10-11 0.19804155595694084 1107 12-13 0.28679659457127826 1107 14-15 0.3241095085857921 1107 16-17 0.3244829981884436 1107 18-19 0.41274411146997636 1107 20-21 0.24292379573482492 1107 22-23 0.2338833710436674 1107 24-25 0.29011869014041736 1107 26-27 0.27786112868800217 1107 28-29 0.2814004475253853 1107 30-31 0.2317202175421258 1107 32-33 0.4291880109753947 1107 34-35 0.3678689347722752 1107 36-37 0.4611835102509829 1107 38-39 0.5731006979836337 1107 40-41 0.5105868932327198 1107 42-43 0.6400188963766098 1107 44-45 0.5415991990454003 1107 46-47 0.511954803909795 1107 48-49 0.5092265001394338 1107 50-51 0.5214569845349644 1107 52-53 0.47478515651665987 1107 54-55 0.5742408052075305 1107 56-57 0.588254697481176 1107 58-59 0.5822589766678377 1107 60-61 0.6267301667799785 1107 62-63 0.7301463286067218 1107 64-65 0.9931111287653813 1107 66-67 1.0895467344459462 1107 68-69 1.1185801627240934 1107 70-71 1.1838786282729679 1107 72-73 1.2242898929770512 1107 74-75 1.380312573495317 1107 76-77 1.3286497120782244 1107 78-79 1.6535857653566133 1107 80-81 1.790915940621339 1107 82-83 1.8398707987735214 1107 84-85 2.0438539866420236 1107 86-87 2.3572857066013135 1107 88-89 2.321666803387508 1107 90-91 2.481679819043759 1107 92-93 2.6515788221693164 1107 94-95 2.8973377610889095 1107 96-97 3.2181153913269185 1107 98-99 3.5158542917061233 1107 100-101 3.8868511255114093 1108 1 0.22011797370108965 1108 2 0.18434028091059673 1108 3 0.1880592345374481 1108 4 0.10539958444249464 1108 5 0.09807989369860337 1108 6 0.09559494977425231 1108 7 0.10009792221352853 1108 8 0.22769219972136057 1108 9 0.12259494453066822 1108 10-11 0.13100360789318444 1108 12-13 0.24219025284293139 1108 14-15 0.2794744326770271 1108 16-17 0.2895829678754609 1108 18-19 0.4278374501922855 1108 20-21 0.2558590712040427 1108 22-23 0.27633922776430353 1108 24-25 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2.5410179540375673 1108 92-93 2.71817660048038 1108 94-95 2.9391540454752025 1108 96-97 3.1629148204324338 1108 98-99 3.3052043308603913 1108 100-101 3.7621771479430173 1109 1 0.22429607684160047 1109 2 0.29553246904092134 1109 3 0.24982904699245978 1109 4 0.12594502844134325 1109 5 0.18159120097655546 1109 6 0.1686390820392134 1109 7 0.18547004595683347 1109 8 0.21887721616088385 1109 9 0.3521460678758359 1109 10-11 0.3462827039964793 1109 12-13 0.34243836170497843 1109 14-15 0.48321060014655615 1109 16-17 0.5045267149257171 1109 18-19 0.47633024158835724 1109 20-21 0.5086394197165731 1109 22-23 0.49271632423145917 1109 24-25 0.4681177669298151 1109 26-27 0.49301060734553914 1109 28-29 0.6365588867564043 1109 30-31 0.5111387306300017 1109 32-33 0.6108955432877607 1109 34-35 0.5661935790646595 1109 36-37 0.6737262766244001 1109 38-39 0.7735704492598359 1109 40-41 0.6981391632446687 1109 42-43 0.6848312673986641 1109 44-45 0.7497831894874523 1109 46-47 0.8446683642443205 1109 48-49 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-0.27281509691269434 2316 58-59 -0.13617666933412664 2316 60-61 0.14096616947014695 2316 62-63 0.13575110726787898 2316 64-65 0.11262239386757855 2316 66-67 0.19850237059995735 2316 68-69 0.1628113014842114 2316 70-71 0.2703219244562831 2316 72-73 0.5669623222999469 2316 74-75 0.7297590762695556 2316 76-77 0.6763076697079882 2316 78-79 0.8300030605311157 2316 80-81 0.932315191215821 2316 82-83 1.1434714581062622 2316 84-85 1.3615517864642825 2316 86-87 1.6305807790198301 2316 88-89 1.6935418784481548 2316 90-91 1.725065024289595 2316 92-93 1.701616502615117 2316 94-95 2.280453168337967 2316 96-97 2.355517812483214 2316 98-99 2.773858759315953 2316 100-101 3.147295814820371 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 18.0 4 47.0 5 98.0 6 223.0 7 572.0 8 1281.0 9 2137.0 10 3464.0 11 5557.0 12 7959.0 13 11213.0 14 14373.0 15 19473.0 16 25006.0 17 31576.0 18 29708.0 19 23328.0 20 22989.0 21 24300.0 22 26567.0 23 29679.0 24 34445.0 25 39900.0 26 47124.0 27 55747.0 28 66854.0 29 78632.0 30 93287.0 31 109705.0 32 130356.0 33 158282.0 34 196118.0 35 251477.0 36 312333.0 37 348548.0 38 192689.0 39 11130.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.530837004405285 19.749646745906407 15.583409525392735 22.13610672429557 2 15.848795364820786 20.95639031468742 39.61219665833016 23.582617662161628 3 19.19377341090526 25.39663211283199 30.018822232759828 25.390772243502923 4 13.206876732243067 17.400666029147192 36.36196643588794 33.0304908027218 5 13.50109637673593 38.113350087173494 33.540475250506795 14.84507828558378 6 31.5998538775439 36.327056634088336 17.26002939904023 14.813060089327534 7 28.52666921840694 33.4307067447815 20.032200136231346 18.010423900580207 8 26.74086661920596 34.98277990907659 19.2477270186489 19.02862645306855 9 26.980081880108937 16.54566680007198 19.964707852752117 36.50954346706697 10-11 25.11298931386031 27.107606554897117 26.478939207489315 21.30046492375325 12-13 26.229936734296093 24.52825013371701 27.25489267106115 21.98692046092575 14-15 23.04620436946041 25.62647509497336 26.216513084318393 25.11080745124784 16-17 21.866938796883552 28.97008604019365 26.698060552713592 22.464914610209206 18-19 21.891853589953627 26.967447856600508 29.745291436584058 21.395407116861808 20-21 23.013102396682573 25.858999310531416 29.87125763807762 21.2566406547084 22-23 22.573155775431893 25.937255449565516 29.753063023413258 21.73652575158933 24-25 22.11405590043268 26.173409952991705 29.974033433201548 21.73850071337407 26-27 21.714911079346724 26.570913529979727 29.874945394859076 21.83922999581447 28-29 21.72320599983127 26.617788788218437 29.863922423770916 21.795082788179375 30-31 21.916228112281562 26.30422396133989 29.8613457479238 21.918202178454752 32-33 21.727744937688797 26.338127706952303 29.700062983427937 22.234064371930963 34-35 22.239040102018464 26.505555858060326 29.557297890673485 21.698106149247725 36-37 22.307539492052964 26.125953143591936 29.475852587384573 22.09065477697053 38-39 21.847253130011115 26.444198475249614 29.546610792958315 22.161937601780952 40-41 22.294069752508396 26.206358963616694 29.552318953159595 21.947252330715315 42-43 22.02434091747878 26.27891019055124 29.347812534288252 22.34893635768173 44-45 21.975695051255425 26.554447993473353 29.302069646534868 22.167787308736354 46-47 22.264524977142383 26.728326822375532 28.59660460477101 22.41054359571108 48-49 22.196129915996213 26.768607443850286 28.584104154224853 22.451158485928648 50-51 22.46262884937709 27.02043594861776 28.19571182956037 22.32122337244478 52-53 22.526297678373503 27.02486201277449 28.025256410416254 22.42358389843575 54-55 22.28916038186336 27.067481062471504 28.187337633057254 22.456020922607877 56-57 22.26035979537869 27.188564047641904 28.016113367087787 22.53496278989162 58-59 22.329285874288974 26.821761773018967 28.055594690551622 22.793357662140444 60-61 22.217886203189178 27.038784374206475 28.20175870594352 22.54157071666083 62-63 22.113613949956797 27.0130591750232 28.25634683054325 22.61698004447675 64-65 22.030121340652755 26.950408548579276 28.11955443456303 22.899915676204937 66-67 22.10839564299561 26.993142075588654 27.967710008480196 22.930752272935543 68-69 22.188358265835728 26.922489497428824 28.005110970778986 22.88404126595646 70-71 22.141708028547907 26.906168686655853 27.88102490190969 23.07109838288655 72-73 22.206810652975374 26.999012135727646 27.692802887206845 23.10137432409013 74-75 22.051295797815726 27.293314232684633 27.493796237305197 23.161593732194444 76-77 22.085956243888187 26.954813833092082 27.86635447329629 23.092875449723444 78-79 22.137739116557594 26.987853466938134 27.84877489453292 23.025632521971357 80-81 21.90251809855413 27.240726614428016 27.698211350217903 23.158543936799948 82-83 22.120424124722227 27.162443026890355 27.521560116682924 23.1955727317045 84-85 22.15149903713258 26.96342558448972 27.54010700743466 23.344968370943036 86-87 22.009100386653184 27.109900977137237 27.508772790188857 23.37222584602073 88-89 22.071553516562844 27.08876115400264 27.399520987250014 23.4401643421845 90-91 22.0803881345338 26.96265776069581 27.472512401420346 23.484441703350043 92-93 21.879142305055684 26.83506127001008 27.49336244154928 23.79243398338496 94-95 21.828621133479707 26.879840877798657 27.462107280314484 23.829430708407155 96-97 21.932632366386834 26.782125342864266 27.326843155182445 23.958399135566452 98-99 21.819124836204455 26.73116252549143 27.236087093306004 24.21362554499811 100-101 21.919248003683844 26.596554209116352 27.36349536319364 24.12070242400616 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1550.0 1 1013.0 2 679.0 3 894.5 4 774.5 5 738.5 6 1303.0 7 2813.5 8 4314.0 9 4765.0 10 4017.5 11 3183.5 12 3124.0 13 3289.5 14 3568.0 15 3689.5 16 3907.5 17 4199.0 18 4371.5 19 4697.5 20 5174.0 21 5831.5 22 6610.5 23 7711.5 24 9220.0 25 11250.5 26 14254.0 27 17638.0 28 20409.0 29 23149.5 30 26830.0 31 31238.5 32 36336.0 33 40720.5 34 44621.0 35 48511.0 36 53268.0 37 57896.0 38 61011.0 39 63591.0 40 66623.5 41 72087.0 42 76556.0 43 79891.5 44 83007.0 45 85867.5 46 88082.5 47 90060.0 48 91216.0 49 90286.0 50 89417.0 51 86372.0 52 81972.5 53 80264.5 54 80120.5 55 74981.0 56 67269.5 57 61442.0 58 57040.5 59 51942.0 60 44055.0 61 36112.5 62 29472.5 63 22849.0 64 17343.0 65 13819.5 66 10601.5 67 8260.0 68 6323.5 69 4722.0 70 3758.5 71 2850.5 72 2306.5 73 2081.5 74 1679.5 75 1261.0 76 944.0 77 663.5 78 475.5 79 328.0 80 190.0 81 100.0 82 53.5 83 22.5 84 13.0 85 8.0 86 8.0 87 5.5 88 3.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.1559287856667E-5 2 0.046380165248040374 3 1.66237151426668E-4 4 0.0 5 0.09866174937172748 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00124677863570001 26-27 0.012135312054146766 28-29 0.0 30-31 0.0 32-33 5.194910982083376E-4 34-35 0.015044462204113453 36-37 0.003490980179960028 38-39 0.012426227069143434 40-41 0.007480671814200061 42-43 0.005361148133510043 44-45 0.002722133354611689 46-47 4.1559287856667E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.701353710683355E-4 68-69 1.870167953550015E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.07796439283335E-5 82-83 9.143043328466741E-4 84-85 0.004426064156735036 86-87 0.0016415918703383467 88-89 8.3118575713334E-5 90-91 0.0010389821964166752 92-93 3.116946589250025E-4 94-95 0.0 96-97 4.1559287856667E-5 98-99 0.0 100-101 7.896264692766731E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2406201.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.95578448804939 #Duplication Level Percentage of deduplicated Percentage of total 1 71.83245882036249 35.16614373256349 2 15.010715532618596 14.69722709252583 3 5.423554702053345 7.965431254586114 4 2.561957446156671 5.016905464063976 5 1.415144023813209 3.4639742924675248 6 0.8410958013806779 2.4705902871717393 7 0.544541408918667 1.8660916301888726 8 0.382831299552151 1.499344527692399 9 0.29107583653924785 1.2824861330964696 >10 1.4999622864748157 13.503689500342878 >50 0.1276588169852281 4.331135240479565 >100 0.06423819378377466 5.626766731078305 >500 0.0038296859151650113 1.2589428122193502 >1k 6.80833051584682E-4 0.5534148782045102 >5k 8.510413144808525E-5 0.3197248946278259 >10k+ 1.702082628961705E-4 0.9781315286913563 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12623 0.5246028906147076 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10854 0.4510845103962636 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7674 0.3189259750120626 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2668 0.11088018000158756 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.1559287856667E-5 0.0 20-21 0.0 0.0 0.0 1.66237151426668E-4 0.0 22-23 0.0 0.0 0.0 3.9481323463833653E-4 0.0 24-25 0.0 0.0 0.0 9.97422908560008E-4 0.0 26-27 0.0 0.0 0.0 0.0023480997639016856 0.0 28-29 0.0 0.0 0.0 0.0063793506859983855 0.0 30-31 0.0 0.0 0.0 0.017953612354080144 0.0 32-33 0.0 0.0 0.0 0.03896183236562532 0.0 34-35 0.0 0.0 0.0 0.0696533664477739 0.0 36-37 0.0 0.0 0.0 0.12085440908718764 0.0 38-39 0.0 0.0 0.0 0.1969286855088166 0.0 40-41 0.0 0.0 0.0 0.2817719716682023 0.0 42-43 0.0 0.0 0.0 0.363186616579413 0.0 44-45 0.0 0.0 0.0 0.46826927592499545 0.0 46-47 0.0 0.0 0.0 0.5923029705332181 0.0 48-49 0.0 0.0 0.0 0.7277239100141676 0.0 50-51 0.0 0.0 0.0 0.8732229768003588 0.0 52-53 0.0 0.0 0.0 1.0191376364651166 0.0 54-55 0.0 0.0 0.0 1.1758577109726078 0.0 56-57 0.0 0.0 0.0 1.3616485073358375 0.0 58-59 0.0 0.0 0.0 1.5624006473274676 0.0 60-61 0.0 0.0 0.0 1.7827687711874445 0.0 62-63 0.0 0.0 0.0 2.008414924605218 0.0 64-65 0.0 0.0 0.0 2.2313597243123082 0.0 66-67 2.07796439283335E-5 2.07796439283335E-5 0.0 2.4894636815461384 0.0 68-69 4.1559287856667E-5 4.1559287856667E-5 0.0 2.7771370720899875 0.0 70-71 4.1559287856667E-5 4.1559287856667E-5 0.0 3.090016170718905 0.0 72-73 4.1559287856667E-5 4.1559287856667E-5 0.0 3.408588891784186 0.0 74-75 4.1559287856667E-5 4.1559287856667E-5 0.0 3.731650015937987 2.07796439283335E-5 76-77 4.1559287856667E-5 4.1559287856667E-5 0.0 4.080498678206849 4.1559287856667E-5 78-79 4.1559287856667E-5 4.1559287856667E-5 0.0 4.470200120438816 4.1559287856667E-5 80-81 4.1559287856667E-5 4.1559287856667E-5 0.0 4.889055403102235 4.1559287856667E-5 82-83 4.1559287856667E-5 4.1559287856667E-5 0.0 5.327048737823648 4.1559287856667E-5 84-85 4.1559287856667E-5 4.1559287856667E-5 0.0 5.761218618062248 4.1559287856667E-5 86-87 4.1559287856667E-5 4.1559287856667E-5 0.0 6.204780897356455 6.23389317850005E-5 88-89 4.1559287856667E-5 4.1559287856667E-5 0.0 6.6900271423709 8.3118575713334E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5830 0.0 60.13161 1 GTATCAA 11340 0.0 45.34366 1 TATCAAC 15235 0.0 33.62615 2 ATCAACG 15375 0.0 33.273785 3 TCAACGC 15655 0.0 32.678665 4 CAACGCA 16135 0.0 31.794907 5 TGGTATC 2385 0.0 31.711197 2 AACGCAG 16685 0.0 30.828106 6 CTATACG 70 0.00835466 27.168589 4 GTGGTAT 2865 0.0 26.896406 1 ACGCAGA 19395 0.0 26.471619 7 CGCAGAG 19505 0.0 26.200579 8 GCAGAGT 20985 0.0 24.171682 9 GTACATG 15615 0.0 21.963278 1 TACATGG 15375 0.0 21.842054 2 GAGTACT 11070 0.0 21.687931 12-13 ACATGGG 15430 0.0 21.168783 3 AGTACTT 11650 0.0 21.036251 12-13 AGAGTAC 20145 0.0 20.853317 10-11 CAGAGTA 20165 0.0 20.703093 10-11 >>END_MODULE