FastQCFastQC Report
Thu 26 May 2016
SRR938656_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938656_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405651
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124550.5177392730699507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98880.4110321904548914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67650.28121286088464204No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25180.10467021193015945No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57600.060.043911
GTATCAA111250.045.7956961
TATCAAC153000.033.0212142
TGGTATC23500.032.9663542
ATCAACG153050.032.9483153
TCAACGC156050.032.2844434
CAACGCA159000.031.6847955
AACGCAG164400.030.666586
GTGGTAT29950.027.077311
ACGCAGA187850.026.7293957
CGCAGAG190000.026.4269318
TACATGG153150.025.1683672
GTACATG157400.025.0036071
GCAGAGT202150.024.3680829
ACATGGG157650.023.4852223
AGTACTT112450.021.96646712-13
GAGTACT107500.021.91742712-13
AGAGTAC194000.021.44935210-11
CAGAGTA196000.021.0123610-11
GTACTTT112900.020.70275314-15
Produced by FastQC (version 0.11.5)